Chris Mulligan
@chrismulligan.bsky.social
Scientist interested in how stuff gets across biological membranes. Also running, woodwork and gardening.
Reposted by Chris Mulligan
Kat Blow has developed CCD2MD, a suite of packages for preparing co-folded outputs for MD simulations.
Created for protein–lipid interactions in integral membrane proteins, it also works for peripheral and lipid-anchored proteins too.
pubs.acs.org/doi/full/10....
github.com/keb721/ccd2md
Created for protein–lipid interactions in integral membrane proteins, it also works for peripheral and lipid-anchored proteins too.
pubs.acs.org/doi/full/10....
github.com/keb721/ccd2md
CCD2MD: A Suite of Packages for Preparing Co-Folded Outputs for Molecular Dynamics Simulations
Protein–lipid interactions play a crucial role in the stability and function of membrane proteins. While experimental approaches to characterize these interactions in a native-like membrane environmen...
pubs.acs.org
November 12, 2025 at 11:53 AM
Kat Blow has developed CCD2MD, a suite of packages for preparing co-folded outputs for MD simulations.
Created for protein–lipid interactions in integral membrane proteins, it also works for peripheral and lipid-anchored proteins too.
pubs.acs.org/doi/full/10....
github.com/keb721/ccd2md
Created for protein–lipid interactions in integral membrane proteins, it also works for peripheral and lipid-anchored proteins too.
pubs.acs.org/doi/full/10....
github.com/keb721/ccd2md
Reposted by Chris Mulligan
🧪How does an ABC transporter 'know' nucleotides & substrates bound to far apart binding sites? Using 1H15N, 19F NMR, HDX-MS, PET-FCS & activity assays with Neuweiler, Marcoux, Orelle & Jault labs, we found a communication hinge! 😍🥳
@microverse.bsky.social @lifeprofile.bsky.social
rdcu.be/ePm4C
@microverse.bsky.social @lifeprofile.bsky.social
rdcu.be/ePm4C
Bidirectional communication between nucleotide and substrate binding sites in a type IV multidrug ABC transporter
Nature Communications - The communication hinge, found in type IV ABC transporters, forms a bidirectional communication between ATP- and substrate-binding sites. This is an unexplored allosteric...
rdcu.be
November 12, 2025 at 11:15 AM
🧪How does an ABC transporter 'know' nucleotides & substrates bound to far apart binding sites? Using 1H15N, 19F NMR, HDX-MS, PET-FCS & activity assays with Neuweiler, Marcoux, Orelle & Jault labs, we found a communication hinge! 😍🥳
@microverse.bsky.social @lifeprofile.bsky.social
rdcu.be/ePm4C
@microverse.bsky.social @lifeprofile.bsky.social
rdcu.be/ePm4C
Reposted by Chris Mulligan
Check out our new paper:
🔗 www.cell.com/structure/fu...
We explored how sulfate-reducing bacteria import isethionate, a sulfur-containing molecule found in the environment and produced by microbes in the human gut. We captured a structure of the IseQM TRAP transporter in a substrate-bound state.
🔗 www.cell.com/structure/fu...
We explored how sulfate-reducing bacteria import isethionate, a sulfur-containing molecule found in the environment and produced by microbes in the human gut. We captured a structure of the IseQM TRAP transporter in a substrate-bound state.
Structural basis of isethionate transport by a TRAP transporter from a sulfate-reducing bacterium
Newton-Vesty et al. used a megabody fiducial to determine the cryo-EM structure of
an isethionate TRAP transporter, revealing the substrate and Na+-binding sites. Transport
is dependent on Na+ and the...
www.cell.com
November 9, 2025 at 8:45 PM
Check out our new paper:
🔗 www.cell.com/structure/fu...
We explored how sulfate-reducing bacteria import isethionate, a sulfur-containing molecule found in the environment and produced by microbes in the human gut. We captured a structure of the IseQM TRAP transporter in a substrate-bound state.
🔗 www.cell.com/structure/fu...
We explored how sulfate-reducing bacteria import isethionate, a sulfur-containing molecule found in the environment and produced by microbes in the human gut. We captured a structure of the IseQM TRAP transporter in a substrate-bound state.
Reposted by Chris Mulligan
If you’re interested in metals, microbes and maths 🤘 - please check out this PhD on the impact of metals on microbial communities with @thecopperdoctor.bsky.social @blindmath.bsky.social Denis Patterson and myself, you’d be based in Durham & be part of a vibrant interdisciplinary research group
We're advertising a PhD at the intersection of mathematical ecology, metallobiology, and microbiology to study the impact of metals on microbial communities with @thecopperdoctor.bsky.social @kateduncan.bsky.social and Denis Patterson. Come join us in lovely Durham!
iapetus.ac.uk/studentships...
iapetus.ac.uk/studentships...
When nutrients turn toxic: how metals shape microbial coexistence
iapetus.ac.uk
October 29, 2025 at 2:49 PM
If you’re interested in metals, microbes and maths 🤘 - please check out this PhD on the impact of metals on microbial communities with @thecopperdoctor.bsky.social @blindmath.bsky.social Denis Patterson and myself, you’d be based in Durham & be part of a vibrant interdisciplinary research group
Reposted by Chris Mulligan
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
October 29, 2025 at 5:11 PM
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
Reposted by Chris Mulligan
Biochemical Society's Membrane Protein Conference
7–9 April 2026
Birmingham, UK | Leonardo Royal Hotel
- Abstracts welcome for talks & posters
- Bursaries available!
- Register & submit here:
www.eventsforce.net/biochemsoc/f...
#MembraneProteins #CallForAbstracts @biochemsoc.bsky.social
7–9 April 2026
Birmingham, UK | Leonardo Royal Hotel
- Abstracts welcome for talks & posters
- Bursaries available!
- Register & submit here:
www.eventsforce.net/biochemsoc/f...
#MembraneProteins #CallForAbstracts @biochemsoc.bsky.social
October 24, 2025 at 8:07 AM
Biochemical Society's Membrane Protein Conference
7–9 April 2026
Birmingham, UK | Leonardo Royal Hotel
- Abstracts welcome for talks & posters
- Bursaries available!
- Register & submit here:
www.eventsforce.net/biochemsoc/f...
#MembraneProteins #CallForAbstracts @biochemsoc.bsky.social
7–9 April 2026
Birmingham, UK | Leonardo Royal Hotel
- Abstracts welcome for talks & posters
- Bursaries available!
- Register & submit here:
www.eventsforce.net/biochemsoc/f...
#MembraneProteins #CallForAbstracts @biochemsoc.bsky.social
Reposted by Chris Mulligan
Check it out! A proper team effort from past and present group members and a great collaboration with @sergemostowylab.bsky.social
The Staphylococcus aureus LXG-domain toxins EsxX and SAR0287 do not promote virulence in a zebrafish larval infection model https://www.biorxiv.org/content/10.1101/2025.10.04.680436v1
October 5, 2025 at 8:11 AM
Check it out! A proper team effort from past and present group members and a great collaboration with @sergemostowylab.bsky.social
Reposted by Chris Mulligan
Mechanism of phosphoinositide regulation of lysosomal pH via inhibition of CLC-7 https://www.biorxiv.org/content/10.1101/2025.10.01.679551v1
October 2, 2025 at 1:45 PM
Mechanism of phosphoinositide regulation of lysosomal pH via inhibition of CLC-7 https://www.biorxiv.org/content/10.1101/2025.10.01.679551v1
Reposted by Chris Mulligan
Our work deciphering the substrate preferences of a di-/tripeptide transporter from S. aureus is now available as a proof at @plos.org Pathogens! Big thanks to the reviewers and to all the brilliant co-authors, particularly Callum who helped get those last few crucial experiments across the line
Defined roles for the Staphylococcus aureus POT transporter DtpT in di/tripeptide uptake and glutathione utilisation inside human macrophages
Author summary The environments where bacterial pathogens thrive are often rich in proteins and their degradation products, including oligopeptides, which can be taken up by the bacterium and used as ...
journals.plos.org
September 30, 2025 at 7:30 AM
Our work deciphering the substrate preferences of a di-/tripeptide transporter from S. aureus is now available as a proof at @plos.org Pathogens! Big thanks to the reviewers and to all the brilliant co-authors, particularly Callum who helped get those last few crucial experiments across the line
Reposted by Chris Mulligan
The outer membrane of Gram-negative bacteria blocks many antibiotics. Our latest work reveals that L-type pyocins bypass this barrier by inactivating the BAM complex, killing Pseudomonas aeruginosa without entering the cell, providing a new blueprint for beating antibiotic resistance.
A Protein Antibiotic Inhibits the BAM Complex to Kill Without Cell Entry
Many antibiotics are ineffective against Gram-negative pathogens such as Pseudomonas aeruginosa because they cannot penetrate the bacterial outer membrane. Here, we show that protein antibiotics calle...
www.biorxiv.org
September 20, 2025 at 9:29 AM
The outer membrane of Gram-negative bacteria blocks many antibiotics. Our latest work reveals that L-type pyocins bypass this barrier by inactivating the BAM complex, killing Pseudomonas aeruginosa without entering the cell, providing a new blueprint for beating antibiotic resistance.
Reposted by Chris Mulligan
New preprint from the lab, in collaboration with Ed Tate, Nate Traaseth and many others!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Small molecule inhibitors of the NorA multidrug efflux pump potentiate antibiotic activity by binding the outward-open conformation
Antibiotic resistance is among the greatest threats of the modern era. Multidrug efflux pumps expel antibiotics from bacterial cells and present a particular challenge by conferring resistance to a br...
www.biorxiv.org
August 23, 2025 at 11:24 AM
New preprint from the lab, in collaboration with Ed Tate, Nate Traaseth and many others!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Chris Mulligan
We are hiring a Postdoc in membrane protein cryo-EM at the University of Georgia. Looking for candidates with expertise in membrane protein purification and biochemistry. Fully funded for up to 5 years. Join us in Athens, GA. Apply: camilo.perez@uga.edu
August 21, 2025 at 2:26 PM
We are hiring a Postdoc in membrane protein cryo-EM at the University of Georgia. Looking for candidates with expertise in membrane protein purification and biochemistry. Fully funded for up to 5 years. Join us in Athens, GA. Apply: camilo.perez@uga.edu
Really pleased to share some recent work from our lab now published in Comms Bio. Here, we’ve identified and characterised a peculiar TRAP transporter binding protein from B. pertussis that we think uses a membrane component from a completely different transporter family.
rdcu.be/eAr8v
rdcu.be/eAr8v
A new class of binding-protein dependent solute transporter exemplified by the TAXI-GltS system from Bordetella pertussis
Communications Biology - Identification and characterization of an unusual tri-domain TRAP transporter binding protein from Bordetella pertussis reveals insight into the diversity of binding...
rdcu.be
August 18, 2025 at 3:24 PM
Really pleased to share some recent work from our lab now published in Comms Bio. Here, we’ve identified and characterised a peculiar TRAP transporter binding protein from B. pertussis that we think uses a membrane component from a completely different transporter family.
rdcu.be/eAr8v
rdcu.be/eAr8v
Reposted by Chris Mulligan
Di-Gembodies (dimerized nanobodies) for improved and duplexed CryoEM of small and challenging targets!
A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social
Out now in Nature Chemical Biology @natchembio.nature.com
www.nature.com/articles/s41...
A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social
Out now in Nature Chemical Biology @natchembio.nature.com
www.nature.com/articles/s41...
Covalently constrained ‘Di-Gembodies’ enable parallel structure solutions by cryo-EM - Nature Chemical Biology
Disulfide-based dimerization of modified identical and heterologous nanobody scaffolds enables higher-order assembly for high-resolution cryo-electron microscopy structure determination that is widely...
www.nature.com
August 15, 2025 at 10:00 AM
Di-Gembodies (dimerized nanobodies) for improved and duplexed CryoEM of small and challenging targets!
A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social
Out now in Nature Chemical Biology @natchembio.nature.com
www.nature.com/articles/s41...
A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social
Out now in Nature Chemical Biology @natchembio.nature.com
www.nature.com/articles/s41...
Reposted by Chris Mulligan
Exciting new paper from Wonsik Lee's group in Korea, using TnSeq to study gene essentiality in various intracellular and extracellular infection niches of S aureus. Loads of cool insights into metabolism and nutrient acquisition in the host
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Environmental cues in different host niches shape the survival fitness of Staphylococcus aureus - Nature Communications
Staphylococcus aureus adapts to diverse host environments during infection. Here, the authors use transposon sequencing to reveal niche-specific genes and identify 27 core genes required for S. aureus...
www.nature.com
August 1, 2025 at 2:46 PM
Exciting new paper from Wonsik Lee's group in Korea, using TnSeq to study gene essentiality in various intracellular and extracellular infection niches of S aureus. Loads of cool insights into metabolism and nutrient acquisition in the host
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Chris Mulligan
Our review on the peculiar properties of mitochondrial carriers of the SLC25 family out: portlandpress.com/biochemj/art...
July 25, 2025 at 11:29 AM
Our review on the peculiar properties of mitochondrial carriers of the SLC25 family out: portlandpress.com/biochemj/art...
Reposted by Chris Mulligan
Just seen this very clever paper from Alex O'Neill's group at Leeds. Systematic approaches to identify routes of antibiotic uptake in S. aureus and E. coli! As someone who never believed the dogma of passive diffusion, I feel very vindicated.. pubmed.ncbi.nlm.nih.gov/40631918/
Carrier-mediated transport as a common route of antibiotic ingress into bacteria - PubMed
Increasing antibiotic resistance among pathogenic bacteria is undermining our ability to treat infection, and new antibacterial drugs are urgently needed to address the problem. One of the most signif...
pubmed.ncbi.nlm.nih.gov
July 13, 2025 at 2:36 PM
Just seen this very clever paper from Alex O'Neill's group at Leeds. Systematic approaches to identify routes of antibiotic uptake in S. aureus and E. coli! As someone who never believed the dogma of passive diffusion, I feel very vindicated.. pubmed.ncbi.nlm.nih.gov/40631918/
Reposted by Chris Mulligan
Super excited to share our latest work revealing impt of protein-lipid interactions for the pri fn of the Tol-Pal complex in maintaining outer memb lipid homeostasis in E. coli! Huge discovery by Nadege Lim in collab @pstansfeld.bsky.social @robincorey.bsky.social 1/7
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org
June 17, 2025 at 11:03 AM
Super excited to share our latest work revealing impt of protein-lipid interactions for the pri fn of the Tol-Pal complex in maintaining outer memb lipid homeostasis in E. coli! Huge discovery by Nadege Lim in collab @pstansfeld.bsky.social @robincorey.bsky.social 1/7
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Chris Mulligan
Join our group in the Centre for Medicines Discovery at Oxford Univ. @cmd.ox.ac.uk @ox.ac.uk !!!!
Happy to chat about any questions about the role and science. Oxford will assist with a skilled worker visa.
Please RT!
my.corehr.com/pls/uoxrecru...
Happy to chat about any questions about the role and science. Oxford will assist with a skilled worker visa.
Please RT!
my.corehr.com/pls/uoxrecru...
June 3, 2025 at 11:18 AM
Join our group in the Centre for Medicines Discovery at Oxford Univ. @cmd.ox.ac.uk @ox.ac.uk !!!!
Happy to chat about any questions about the role and science. Oxford will assist with a skilled worker visa.
Please RT!
my.corehr.com/pls/uoxrecru...
Happy to chat about any questions about the role and science. Oxford will assist with a skilled worker visa.
Please RT!
my.corehr.com/pls/uoxrecru...
Reposted by Chris Mulligan
Really excited that our group was able to contribute to this awesome work from Bing Zhang and Zihe Rao solving the structure and assembly of the MmpS5L5 efflux pump from Mtb!!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structure and assembly of the MmpL5/MmpS5 efflux transporter from Mycobacterium tuberculosis - Nature Communications
The MmpL5/MmpS5 efflux system is crucial for drug extrusion and siderophore export in M. tuberculosis. Here, the authors report its high-resolution cryo-EM structures, revealing a unique trimeric asse...
www.nature.com
May 30, 2025 at 5:07 AM
Really excited that our group was able to contribute to this awesome work from Bing Zhang and Zihe Rao solving the structure and assembly of the MmpS5L5 efflux pump from Mtb!!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Chris Mulligan
Beta-lactams covalently bind to Penicillin Binding Proteins and have been the mainstay of antimicrobial chemotherapy for decades.
In this new paper with colleagues @Bicycle_tx we show a new way to hit old targets: rdcu.be/eoaRI
In this new paper with colleagues @Bicycle_tx we show a new way to hit old targets: rdcu.be/eoaRI
Discovery and chemical optimisation of a potent, Bi-cyclic antimicrobial inhibitor of Escherichia coli PBP3
Communications Biology - Discovery of a bicyclic peptide inhibitor of cell wall biosynthesis in the Enterobacterales demonstrates applicability of a modified phage display platform to antibiotic...
rdcu.be
May 28, 2025 at 10:30 AM
Beta-lactams covalently bind to Penicillin Binding Proteins and have been the mainstay of antimicrobial chemotherapy for decades.
In this new paper with colleagues @Bicycle_tx we show a new way to hit old targets: rdcu.be/eoaRI
In this new paper with colleagues @Bicycle_tx we show a new way to hit old targets: rdcu.be/eoaRI
Reposted by Chris Mulligan
Are you wondering how sugar is used to fuel your body? Work with @mitocarriers.bsky.social and Vanessa Leone describes an important step along the way... how the break-down product pyruvate gets into the mitochondria!
A very satisfying application of #alphafold3
www.linkedin.com/posts/nation...
A very satisfying application of #alphafold3
www.linkedin.com/posts/nation...
How sugar fuels ATP production in the mitochondrion | National Institute of Neurological Disorders and Stroke (NINDS) posted on the topic | LinkedIn
Ever wonder why sugar gives us such an energy buzz? Its breakdown product, pyruvate, fuels ATP production in the mitochondrion. Dr. Lucy Forrest, an #NINDSIntramural investigator, and collaborators re...
www.linkedin.com
May 28, 2025 at 12:13 AM
Are you wondering how sugar is used to fuel your body? Work with @mitocarriers.bsky.social and Vanessa Leone describes an important step along the way... how the break-down product pyruvate gets into the mitochondria!
A very satisfying application of #alphafold3
www.linkedin.com/posts/nation...
A very satisfying application of #alphafold3
www.linkedin.com/posts/nation...
Reposted by Chris Mulligan
The Role of Chloride Ions in Serotonin Transport https://www.biorxiv.org/content/10.1101/2025.05.20.654092v1
May 21, 2025 at 5:46 AM
The Role of Chloride Ions in Serotonin Transport https://www.biorxiv.org/content/10.1101/2025.05.20.654092v1
Reposted by Chris Mulligan
New Type VII ABC Transporter just dropped!
We are super excited to present our characterisation of the YbbAP-TesA complex, a novel Type VII ABC transporter linked to a periplasmic thioesterase.
doi.org/10.1101/2025...
We are super excited to present our characterisation of the YbbAP-TesA complex, a novel Type VII ABC transporter linked to a periplasmic thioesterase.
doi.org/10.1101/2025...
May 19, 2025 at 2:34 PM
New Type VII ABC Transporter just dropped!
We are super excited to present our characterisation of the YbbAP-TesA complex, a novel Type VII ABC transporter linked to a periplasmic thioesterase.
doi.org/10.1101/2025...
We are super excited to present our characterisation of the YbbAP-TesA complex, a novel Type VII ABC transporter linked to a periplasmic thioesterase.
doi.org/10.1101/2025...
Reposted by Chris Mulligan
Structure of YbbAP-TesA: a Type VII ABC transporter lipid-hydrolase complex https://www.biorxiv.org/content/10.1101/2025.05.16.654284v1
May 17, 2025 at 3:17 AM
Structure of YbbAP-TesA: a Type VII ABC transporter lipid-hydrolase complex https://www.biorxiv.org/content/10.1101/2025.05.16.654284v1