Shu-Sin Chng
@figlegend.bsky.social
Assoc Professor @ National University of Singapore. 🇸🇬 NUS Assoc Dir for SCELSE. Membrane/lipid biochemist. Educator who teaches chemistry in biology. Advocate (Vice Dean) for students. Horn player. 📯📯
https://chemistry.nus.edu.sg/people/chng-shu-sin/
https://chemistry.nus.edu.sg/people/chng-shu-sin/
Pinned
Shu-Sin Chng
@figlegend.bsky.social
· Jun 17
www.biorxiv.org
Super excited to share our latest work revealing impt of protein-lipid interactions for the pri fn of the Tol-Pal complex in maintaining outer memb lipid homeostasis in E. coli! Huge discovery by Nadege Lim in collab @pstansfeld.bsky.social @robincorey.bsky.social 1/7
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Shu-Sin Chng
An Asgard archaeon with internal membrane compartments
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
November 7, 2025 at 10:44 AM
An Asgard archaeon with internal membrane compartments
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
Reposted by Shu-Sin Chng
New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
October 30, 2025 at 3:21 AM
New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
Reposted by Shu-Sin Chng
🚨Our paper is out! 🥳
Hijacking a bacterial ABC transporter for efficient genetic code expansion.
Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social
www.nature.com/articles/s41...
it all started years ago with a failed experiment
🧵👇 1/9
Hijacking a bacterial ABC transporter for efficient genetic code expansion.
Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social
www.nature.com/articles/s41...
it all started years ago with a failed experiment
🧵👇 1/9
Hijacking a bacterial ABC transporter for genetic code expansion - Nature
Bacterial ATP-binding cassette (ABC) transporters can be utilized and engineered to transport non-canonical amino acids into Escherichia coli for highly efficient synthesis of proteins with novel func...
www.nature.com
October 16, 2025 at 2:06 PM
🚨Our paper is out! 🥳
Hijacking a bacterial ABC transporter for efficient genetic code expansion.
Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social
www.nature.com/articles/s41...
it all started years ago with a failed experiment
🧵👇 1/9
Hijacking a bacterial ABC transporter for efficient genetic code expansion.
Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social
www.nature.com/articles/s41...
it all started years ago with a failed experiment
🧵👇 1/9
Reposted by Shu-Sin Chng
PhD opportunity in my lab: exploring how commensals and pathogens build biofilm communities on endotracheal (ventilator) tubes. Microbial ecology, medical microbiology and fancy imaging! Co-supervised with the fabulous Saskia Bakker and Jeremy Webb warwick.ac.uk/fac/cross_fa... #MicroSky
Dr Freya Harrison
Dr Freya Harrison
warwick.ac.uk
October 15, 2025 at 8:09 PM
PhD opportunity in my lab: exploring how commensals and pathogens build biofilm communities on endotracheal (ventilator) tubes. Microbial ecology, medical microbiology and fancy imaging! Co-supervised with the fabulous Saskia Bakker and Jeremy Webb warwick.ac.uk/fac/cross_fa... #MicroSky
Reposted by Shu-Sin Chng
Attention membrane traffickers! I'm recruiting a post-doc to my lab in Dundee. We're dissecting the functions of intrinsically disordered domains of COPII coat proteins. We think they control timing of coat assembly and morphology of carriers.
www.dundee.ac.uk/work-for-us/...
www.dundee.ac.uk/work-for-us/...
Postdoctoral Research Assistant - MCDB - UOD2016
Closing date: Thursday 6 November 2025, 23:59
www.dundee.ac.uk
October 15, 2025 at 12:53 PM
Attention membrane traffickers! I'm recruiting a post-doc to my lab in Dundee. We're dissecting the functions of intrinsically disordered domains of COPII coat proteins. We think they control timing of coat assembly and morphology of carriers.
www.dundee.ac.uk/work-for-us/...
www.dundee.ac.uk/work-for-us/...
Reposted by Shu-Sin Chng
Persistent LPS insertion, spatial segregation and vesicle biogenesis drive growth-independent adaptation of the Escherichia coli outer membrane https://www.biorxiv.org/content/10.1101/2025.10.09.681453v1
October 10, 2025 at 3:16 AM
Persistent LPS insertion, spatial segregation and vesicle biogenesis drive growth-independent adaptation of the Escherichia coli outer membrane https://www.biorxiv.org/content/10.1101/2025.10.09.681453v1
We are back for #SGBUG for Oct, with Shaun Tan from Benson Chen's grp at GIS A*STAR, and Jason Chen from Chris Sham's grp at NUS ID-TRP, sharing about genetic manipulation in gut commensals, and glycoengineering in S. pneumoniae, respectively!
October 9, 2025 at 9:50 AM
We are back for #SGBUG for Oct, with Shaun Tan from Benson Chen's grp at GIS A*STAR, and Jason Chen from Chris Sham's grp at NUS ID-TRP, sharing about genetic manipulation in gut commensals, and glycoengineering in S. pneumoniae, respectively!
Reposted by Shu-Sin Chng
Come join us in Oxford! Two positions open right now in @oxfordbiochemistry.bsky.social
It’s a great place to work. Really supportive colleagues, great students, colleges provide a unique social environment - in summary, it’s a lot of fun.
It’s a great place to work. Really supportive colleagues, great students, colleges provide a unique social environment - in summary, it’s a lot of fun.
Become my colleague @oxfordbiochemistry.bsky.social !
2 Associate Professorships in the areas of
- prokaryotic/eukaryotic microbiology
- metabolism
my.corehr.com/pls/uoxrecru...
2 Associate Professorships in the areas of
- prokaryotic/eukaryotic microbiology
- metabolism
my.corehr.com/pls/uoxrecru...
Job Details
my.corehr.com
October 1, 2025 at 7:12 AM
Come join us in Oxford! Two positions open right now in @oxfordbiochemistry.bsky.social
It’s a great place to work. Really supportive colleagues, great students, colleges provide a unique social environment - in summary, it’s a lot of fun.
It’s a great place to work. Really supportive colleagues, great students, colleges provide a unique social environment - in summary, it’s a lot of fun.
Reposted by Shu-Sin Chng
Come and work with me and @ariannebabina.bsky.social on #Streptomyces evolution and antibiotic production
Origins of a tangled bank: Adaptation and evolution in antibiotic-producing Streptomyces
www.gla.ac.uk/postgraduate...
please repost
Origins of a tangled bank: Adaptation and evolution in antibiotic-producing Streptomyces
www.gla.ac.uk/postgraduate...
please repost
University of Glasgow - Postgraduate study - Centres for Doctoral Training - NorthWest Biosciences - Our Projects - Underpinning Bioscience - Paul A Hoskisson
www.gla.ac.uk
September 30, 2025 at 11:02 AM
Come and work with me and @ariannebabina.bsky.social on #Streptomyces evolution and antibiotic production
Origins of a tangled bank: Adaptation and evolution in antibiotic-producing Streptomyces
www.gla.ac.uk/postgraduate...
please repost
Origins of a tangled bank: Adaptation and evolution in antibiotic-producing Streptomyces
www.gla.ac.uk/postgraduate...
please repost
Reposted by Shu-Sin Chng
Now published! www.nature.com/articles/s41...
Very nice collaboration with Hoogenboom ( @ucl.ac.uk ) and Bonev ( @uniofnottingham.bsky.social ) labs.
Very nice collaboration with Hoogenboom ( @ucl.ac.uk ) and Bonev ( @uniofnottingham.bsky.social ) labs.
September 29, 2025 at 2:24 PM
Now published! www.nature.com/articles/s41...
Very nice collaboration with Hoogenboom ( @ucl.ac.uk ) and Bonev ( @uniofnottingham.bsky.social ) labs.
Very nice collaboration with Hoogenboom ( @ucl.ac.uk ) and Bonev ( @uniofnottingham.bsky.social ) labs.
Reposted by Shu-Sin Chng
📝New preprint! #MicroSky
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
Dynamics and interplay of gene expression and chromosome organization across a predatory lifecycle
The obligate predatory bacterium Bdellovibrio bacteriovorus alternates between a motile attack phase and a growth phase inside another bacterium, during which it undergoes multiple rounds of DNA repli...
www.biorxiv.org
September 25, 2025 at 7:12 AM
📝New preprint! #MicroSky
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
Reposted by Shu-Sin Chng
Nice work on tackling genetic interactions in the pneumococcus! If you like this, you will also like our work doing the same thing using dual CRISPRi. Coming soon in @cp-cellsystems.bsky.social www.biorxiv.org/content/10.1...
September 26, 2025 at 7:07 AM
Nice work on tackling genetic interactions in the pneumococcus! If you like this, you will also like our work doing the same thing using dual CRISPRi. Coming soon in @cp-cellsystems.bsky.social www.biorxiv.org/content/10.1...
Reposted by Shu-Sin Chng
Dual transposon sequencing profiles the genetic interaction landscape in bacteria | Science www.science.org/doi/10.1126/...
Dual transposon sequencing profiles the genetic interaction landscape in bacteria
Gene redundancy complicates systematic characterization of gene function as single-gene deletions may not produce discernible phenotypes. We report dual transposon sequencing (dual Tn-seq), a platform...
www.science.org
September 25, 2025 at 11:19 PM
Dual transposon sequencing profiles the genetic interaction landscape in bacteria | Science www.science.org/doi/10.1126/...
Reposted by Shu-Sin Chng
The Palmer group are interested in the mechanisms bacteria use to compete with one another during colonisation. Her group are focused on the type vii secretion system and the toxins it exports.
Follow @proftracypalmer.bsky.social for more insights.
www.ncl.ac.uk/medical-scie...
Follow @proftracypalmer.bsky.social for more insights.
www.ncl.ac.uk/medical-scie...
September 22, 2025 at 11:38 AM
The Palmer group are interested in the mechanisms bacteria use to compete with one another during colonisation. Her group are focused on the type vii secretion system and the toxins it exports.
Follow @proftracypalmer.bsky.social for more insights.
www.ncl.ac.uk/medical-scie...
Follow @proftracypalmer.bsky.social for more insights.
www.ncl.ac.uk/medical-scie...
Reposted by Shu-Sin Chng
@jmarlesw.bsky.social is a structural biochemist. His group are interested in how bacteria sense and respond to their environment. Jon is Academic Lead for our Electron Microscopy Facility and is excited for our new Tundra microscope to be up and running.
www.marles-wright-lab.org
www.marles-wright-lab.org
September 22, 2025 at 12:10 PM
@jmarlesw.bsky.social is a structural biochemist. His group are interested in how bacteria sense and respond to their environment. Jon is Academic Lead for our Electron Microscopy Facility and is excited for our new Tundra microscope to be up and running.
www.marles-wright-lab.org
www.marles-wright-lab.org
Reposted by Shu-Sin Chng
The @henrikstrahl.bsky.social group focuses on bacterial cell envelopes and envelope-targeting antibiotics.
Using cell biology, high-end fluorescence microscopy, genetics, and biochemistry, they study the function, biosynthesis and organisation of bacterial cell membranes.
strahllab.weebly.com
Using cell biology, high-end fluorescence microscopy, genetics, and biochemistry, they study the function, biosynthesis and organisation of bacterial cell membranes.
strahllab.weebly.com
September 23, 2025 at 8:58 AM
The @henrikstrahl.bsky.social group focuses on bacterial cell envelopes and envelope-targeting antibiotics.
Using cell biology, high-end fluorescence microscopy, genetics, and biochemistry, they study the function, biosynthesis and organisation of bacterial cell membranes.
strahllab.weebly.com
Using cell biology, high-end fluorescence microscopy, genetics, and biochemistry, they study the function, biosynthesis and organisation of bacterial cell membranes.
strahllab.weebly.com
Reposted by Shu-Sin Chng
Dr Alistair Brown’s group is interested in mycobacterial biology, focusing on antimicrobial drug discovery and elucidation of drug mechanisms of action. They aim to understand how existing and novel compounds affect mycobacterial growth, survival, and persistence.
@mycobacterium-ncl.bsky.social
@mycobacterium-ncl.bsky.social
September 23, 2025 at 9:10 AM
Dr Alistair Brown’s group is interested in mycobacterial biology, focusing on antimicrobial drug discovery and elucidation of drug mechanisms of action. They aim to understand how existing and novel compounds affect mycobacterial growth, survival, and persistence.
@mycobacterium-ncl.bsky.social
@mycobacterium-ncl.bsky.social
Reposted by Shu-Sin Chng
Dr Chien-Yi Chang's lab studies how Gram-negative bacteria sense and respond to different environments using molecular and bioimaging approaches.
👉 @davychang.bsky.social
chienyichang.wixsite.com/changlab
👉 @davychang.bsky.social
chienyichang.wixsite.com/changlab
September 23, 2025 at 9:14 AM
Dr Chien-Yi Chang's lab studies how Gram-negative bacteria sense and respond to different environments using molecular and bioimaging approaches.
👉 @davychang.bsky.social
chienyichang.wixsite.com/changlab
👉 @davychang.bsky.social
chienyichang.wixsite.com/changlab
Reposted by Shu-Sin Chng
The Salgado group focuses on structural and functional characterisation of proteins involved in pathogenicity pathways in C. difficile. They are particularly interested in S-layer and sporulation proteins and their role in infection.
Follow @pssalgado.bsky.social
salgadolab.org
Follow @pssalgado.bsky.social
salgadolab.org
September 23, 2025 at 10:00 AM
The Salgado group focuses on structural and functional characterisation of proteins involved in pathogenicity pathways in C. difficile. They are particularly interested in S-layer and sporulation proteins and their role in infection.
Follow @pssalgado.bsky.social
salgadolab.org
Follow @pssalgado.bsky.social
salgadolab.org
Reposted by Shu-Sin Chng
Next up,
Dr Kate Beckham @kshbeckham.bsky.social
The Beckham lab is interested mycobacterial membrane transport processes. They focusing on understanding the steps occurring at the mycobacterial outer membrane.
www.ncl.ac.uk/cbcb/staff/p...
Dr Kate Beckham @kshbeckham.bsky.social
The Beckham lab is interested mycobacterial membrane transport processes. They focusing on understanding the steps occurring at the mycobacterial outer membrane.
www.ncl.ac.uk/cbcb/staff/p...
September 23, 2025 at 8:53 AM
Next up,
Dr Kate Beckham @kshbeckham.bsky.social
The Beckham lab is interested mycobacterial membrane transport processes. They focusing on understanding the steps occurring at the mycobacterial outer membrane.
www.ncl.ac.uk/cbcb/staff/p...
Dr Kate Beckham @kshbeckham.bsky.social
The Beckham lab is interested mycobacterial membrane transport processes. They focusing on understanding the steps occurring at the mycobacterial outer membrane.
www.ncl.ac.uk/cbcb/staff/p...
Reposted by Shu-Sin Chng
Day 2 of MHD introductions 😃
Dr Lis Lowe @lislowe.bsky.social
The Lowe lab is focussed on microbial glycobiology. They work on bacterial Cazymes from commensals and pathogens, and are particularly interested in enzymes that target microbial glycans such as the cell walls of Mycobacteria.
Dr Lis Lowe @lislowe.bsky.social
The Lowe lab is focussed on microbial glycobiology. They work on bacterial Cazymes from commensals and pathogens, and are particularly interested in enzymes that target microbial glycans such as the cell walls of Mycobacteria.
September 23, 2025 at 8:41 AM
Day 2 of MHD introductions 😃
Dr Lis Lowe @lislowe.bsky.social
The Lowe lab is focussed on microbial glycobiology. They work on bacterial Cazymes from commensals and pathogens, and are particularly interested in enzymes that target microbial glycans such as the cell walls of Mycobacteria.
Dr Lis Lowe @lislowe.bsky.social
The Lowe lab is focussed on microbial glycobiology. They work on bacterial Cazymes from commensals and pathogens, and are particularly interested in enzymes that target microbial glycans such as the cell walls of Mycobacteria.
Reposted by Shu-Sin Chng
Check out this talk on 30 Sep 2025 - Tackling the global spread of AMR using genome-resolved metagenomics and Al - by Dr Niranjan Nagarajan, and hosted by @fillouxlab.bsky.social
September 24, 2025 at 9:13 AM
Check out this talk on 30 Sep 2025 - Tackling the global spread of AMR using genome-resolved metagenomics and Al - by Dr Niranjan Nagarajan, and hosted by @fillouxlab.bsky.social
Reposted by Shu-Sin Chng
A colleague received proofs of an accepted manuscript, and the "proofing process" clearly involved running the text through ChatGPT. Their voice has been erased, and errors introduced. This is from a supposedly reputable publisher. Anyone else been put through this particular form of hell? #MicroSky
September 24, 2025 at 12:19 PM
A colleague received proofs of an accepted manuscript, and the "proofing process" clearly involved running the text through ChatGPT. Their voice has been erased, and errors introduced. This is from a supposedly reputable publisher. Anyone else been put through this particular form of hell? #MicroSky
Reposted by Shu-Sin Chng
PPE51 is one of the best candidates to form a MOM transport channel. However, our data indicate that it is not that simple. PPE51 mutations in Mmarinum (4 close homologs) indeed reduce the growth on glycerol & glucose, but also highly increase permeability.. and no, we do not fully understand this
PPE51 modulates membrane integrity in Mycobacterium marinum | mBio
The impermeable outer membrane of pathogenic mycobacteria presents a major obstacle
to nutrient acquisition and antibiotic penetration. PPE51, a substrate of the ESX-5
secretion system, has previously...
journals.asm.org
September 23, 2025 at 7:48 AM
PPE51 is one of the best candidates to form a MOM transport channel. However, our data indicate that it is not that simple. PPE51 mutations in Mmarinum (4 close homologs) indeed reduce the growth on glycerol & glucose, but also highly increase permeability.. and no, we do not fully understand this
Reposted by Shu-Sin Chng
The outer membrane of Gram-negative bacteria blocks many antibiotics. Our latest work reveals that L-type pyocins bypass this barrier by inactivating the BAM complex, killing Pseudomonas aeruginosa without entering the cell, providing a new blueprint for beating antibiotic resistance.
A Protein Antibiotic Inhibits the BAM Complex to Kill Without Cell Entry
Many antibiotics are ineffective against Gram-negative pathogens such as Pseudomonas aeruginosa because they cannot penetrate the bacterial outer membrane. Here, we show that protein antibiotics calle...
www.biorxiv.org
September 20, 2025 at 9:29 AM
The outer membrane of Gram-negative bacteria blocks many antibiotics. Our latest work reveals that L-type pyocins bypass this barrier by inactivating the BAM complex, killing Pseudomonas aeruginosa without entering the cell, providing a new blueprint for beating antibiotic resistance.