Johnathan Canton
@cantonjohnathan.bsky.social
Assistant Professor at the University of Calgary.
https://vet.ucalgary.ca/labs/cantonlab/home
https://vet.ucalgary.ca/labs/cantonlab/home
Reposted by Johnathan Canton
Delighted that this study is finally out @embojournal.org. A detailed analysis of how DNGR-1/CLEC9A signals to promote cross-presentation of dead cell-associated antigens yet does not activate dendritic cells.
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
DNGR-1 signalling limits dendritic cell activation for optimal antigen cross-presentation | The EMBO Journal
imageimageDNGR-1 (CLEC9A) is a C-type lectin receptor of type-1 conventional dendritic cells (cDC1s) with an important role in cross-presentation of dead cell antigens. This study explores how it equips cDC1s with the ability to cross-present such ...
www.embopress.org
November 3, 2025 at 5:23 PM
Delighted that this study is finally out @embojournal.org. A detailed analysis of how DNGR-1/CLEC9A signals to promote cross-presentation of dead cell-associated antigens yet does not activate dendritic cells.
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
Reposted by Johnathan Canton
@kirstin-rollins.bsky.social, @mmorrissey.bsky.social et al show that #macrophages attack adherent cells using #trogocytosis instead of #phagocytosis. Reducing adhesion increased phagocytosis. Mitotic cells, which disassemble adhesions, are more vulnerable to phagocytosis rupress.org/jcb/article/...
October 3, 2025 at 5:34 PM
@kirstin-rollins.bsky.social, @mmorrissey.bsky.social et al show that #macrophages attack adherent cells using #trogocytosis instead of #phagocytosis. Reducing adhesion increased phagocytosis. Mitotic cells, which disassemble adhesions, are more vulnerable to phagocytosis rupress.org/jcb/article/...
Reposted by Johnathan Canton
Next week's episode features Dr. Johnathan Canton (@cantonjohnathan.bsky.social) from @ucalgary.bsky.social!
His lab identified a specialized set of proteins enabling cytosolic delivery of engulfed antigens for cross-presentation by #DendriticCells.
Read it in #ScienceImmunology: bit.ly/44ExZEp
His lab identified a specialized set of proteins enabling cytosolic delivery of engulfed antigens for cross-presentation by #DendriticCells.
Read it in #ScienceImmunology: bit.ly/44ExZEp
July 14, 2025 at 9:29 PM
Next week's episode features Dr. Johnathan Canton (@cantonjohnathan.bsky.social) from @ucalgary.bsky.social!
His lab identified a specialized set of proteins enabling cytosolic delivery of engulfed antigens for cross-presentation by #DendriticCells.
Read it in #ScienceImmunology: bit.ly/44ExZEp
His lab identified a specialized set of proteins enabling cytosolic delivery of engulfed antigens for cross-presentation by #DendriticCells.
Read it in #ScienceImmunology: bit.ly/44ExZEp
Reposted by Johnathan Canton
We’ve engineered stable cell systems to induce lipid scrambling of the plasma membrane with active forms of TMEM16F. Scrambling alone causes vesiculation (🔵TMEM16F-active; 🟠PS/Annexin V). Let us know if you want to try the cells out. Preprint: www.biorxiv.org/content/10.1...
May 14, 2025 at 10:46 PM
We’ve engineered stable cell systems to induce lipid scrambling of the plasma membrane with active forms of TMEM16F. Scrambling alone causes vesiculation (🔵TMEM16F-active; 🟠PS/Annexin V). Let us know if you want to try the cells out. Preprint: www.biorxiv.org/content/10.1...
Reposted by Johnathan Canton
Wu et al @sickkidsto.bsky.social show that Snx10 regulates PI(3,5)P2 accumulation on #lysosomes by controlling PI(3)P delivery. Deleting Snx10 inhibits ClC-7, thereby impairing (phago)lysosome resolution. This mechanism may account for Snx10-related #osteopetrosis. rupress.org/jcb/article/...
March 26, 2025 at 7:53 PM
Wu et al @sickkidsto.bsky.social show that Snx10 regulates PI(3,5)P2 accumulation on #lysosomes by controlling PI(3)P delivery. Deleting Snx10 inhibits ClC-7, thereby impairing (phago)lysosome resolution. This mechanism may account for Snx10-related #osteopetrosis. rupress.org/jcb/article/...
As usual, a thoughtful and very beautifully illustrated review from the Freeman lab! 🤩
We've been studying mechanisms of resilience that protect lysosomes from damage and wrote a review on the topic. We consider lysosomal stress and damage on a spectrum. Please help me share as this is my first post 😀 and I don't have many followers! 🙏 Open Access: www.embopress.org/doi/full/10....
February 27, 2025 at 7:08 PM
As usual, a thoughtful and very beautifully illustrated review from the Freeman lab! 🤩
Reposted by Johnathan Canton
Wyatt Miller's thesis project is on bioRxiv! We describe the signaling cascade that allows the 'Don't Eat Me' signal CD47 to inhibit phagocytosis. www.biorxiv.org/content/10.1...
CD47 prevents Rac-mediated phagocytosis through Vav1 dephosphorylation
CD47 is expressed by viable cells to protect against phagocytosis. CD47 is recognized by SIRPα, an inhibitory receptor expressed by macrophages and other myeloid cells. Activated SIRPα recruits SHP-1 ...
www.biorxiv.org
February 12, 2025 at 6:42 PM
Wyatt Miller's thesis project is on bioRxiv! We describe the signaling cascade that allows the 'Don't Eat Me' signal CD47 to inhibit phagocytosis. www.biorxiv.org/content/10.1...
Reposted by Johnathan Canton
Always a pleasure to discuss and build a review with Nacho Melero & team!!! #immunosky #cancer #cancerimmunology
February 4, 2025 at 8:07 AM
Always a pleasure to discuss and build a review with Nacho Melero & team!!! #immunosky #cancer #cancerimmunology
Reposted by Johnathan Canton
‘Cholesterol mobilization regulates dendritic cell maturation and the immunogenic response to cancer’
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Cholesterol mobilization regulates dendritic cell maturation and the immunogenic response to cancer - Nature Immunology
To mature, dendritic cells collect and synthesize cholesterol to construct lipid nanodomains on their membranes. Obstructing this process impairs their ability to prime T cells.
www.nature.com
February 3, 2025 at 9:04 PM
‘Cholesterol mobilization regulates dendritic cell maturation and the immunogenic response to cancer’
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Johnathan Canton
Extremely grateful to Damon Runyon for this funding, especially with todays news on NIH grants. See a green macrophage nibbling on a pink cancer cell 👇
January 28, 2025 at 5:25 PM
Extremely grateful to Damon Runyon for this funding, especially with todays news on NIH grants. See a green macrophage nibbling on a pink cancer cell 👇