Adrien Leger
@adrienleger.bsky.social
Director of Modified Bases Research at @nanoporetech.com
EMBL-EBI alumni, keen cyclist and father of two.
Views are my own. Reposts are not endorsement.
EMBL-EBI alumni, keen cyclist and father of two.
Views are my own. Reposts are not endorsement.
Reposted by Adrien Leger
Our abstract submissions for London Calling 2026 are now open! If you’ve been using Oxford Nanopore technology to power the bigger and bolder research questions, we want to hear about it. Submit your abstract here: nanoporetech.com/about/events... #nanoporeconf
October 20, 2025 at 10:11 AM
Our abstract submissions for London Calling 2026 are now open! If you’ve been using Oxford Nanopore technology to power the bigger and bolder research questions, we want to hear about it. Submit your abstract here: nanoporetech.com/about/events... #nanoporeconf
Reposted by Adrien Leger
Excited to welcome @adrienleger.bsky.social from @nanoporetech.com to our RNA Symposium! 🎤 His talk will focus on how nanopore direct RNA sequencing can reveal multiple RNA modifications at once. Secure your spot at our symposium now 👉 zurl.co/mrhKq
#RNA #ribosome #nanopore
#RNA #ribosome #nanopore
August 28, 2025 at 9:00 AM
Excited to welcome @adrienleger.bsky.social from @nanoporetech.com to our RNA Symposium! 🎤 His talk will focus on how nanopore direct RNA sequencing can reveal multiple RNA modifications at once. Secure your spot at our symposium now 👉 zurl.co/mrhKq
#RNA #ribosome #nanopore
#RNA #ribosome #nanopore
Reposted by Adrien Leger
Our paper using Oxford #Nanopore direct RNA sequencing to identify m6A modifications on RNA isoforms in human brain is now out in Science Advances. 🧪
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Isoform-level profiling of m6A epitranscriptomic signatures in human brain
Direct RNA-seq in brain reveals RNA isoform and region-specific m6A modifications, highlighting their role in gene regulation.
www.science.org
August 12, 2025 at 2:30 AM
Our paper using Oxford #Nanopore direct RNA sequencing to identify m6A modifications on RNA isoforms in human brain is now out in Science Advances. 🧪
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
🚨 We’re hiring ! 🚨
Join our cutting-edge research team as a molecular biologist at @nanoporetech.com HQ in Oxford.
Perfect for a fresh PhD or MSc with a couple of years’ experience. Work at the interface of chemistry, molecular/synthetic biology and AI
👉 ejnh.fa.em2.oraclecloud.com/hcmUI/Candid...
Join our cutting-edge research team as a molecular biologist at @nanoporetech.com HQ in Oxford.
Perfect for a fresh PhD or MSc with a couple of years’ experience. Work at the interface of chemistry, molecular/synthetic biology and AI
👉 ejnh.fa.em2.oraclecloud.com/hcmUI/Candid...
June 2, 2025 at 9:54 AM
🚨 We’re hiring ! 🚨
Join our cutting-edge research team as a molecular biologist at @nanoporetech.com HQ in Oxford.
Perfect for a fresh PhD or MSc with a couple of years’ experience. Work at the interface of chemistry, molecular/synthetic biology and AI
👉 ejnh.fa.em2.oraclecloud.com/hcmUI/Candid...
Join our cutting-edge research team as a molecular biologist at @nanoporetech.com HQ in Oxford.
Perfect for a fresh PhD or MSc with a couple of years’ experience. Work at the interface of chemistry, molecular/synthetic biology and AI
👉 ejnh.fa.em2.oraclecloud.com/hcmUI/Candid...
Reposted by Adrien Leger
The world prefers its energy more in the form of electricity than anything else.
May 27, 2025 at 1:25 PM
The world prefers its energy more in the form of electricity than anything else.
Reposted by Adrien Leger
At London Calling, Rosemary Sinclair Dokos and Lakmal Jayasinghe shared how we’re doubling down on what matters: delivering a future-ready platform with proven performance across DNA, RNA, and now proteins. Click here to discover what’s new – and why it matters: nanoporetech.com/news/london-...
May 21, 2025 at 8:36 PM
At London Calling, Rosemary Sinclair Dokos and Lakmal Jayasinghe shared how we’re doubling down on what matters: delivering a future-ready platform with proven performance across DNA, RNA, and now proteins. Click here to discover what’s new – and why it matters: nanoporetech.com/news/london-...
Reposted by Adrien Leger
#NanoporeConf Lakmal Jayasinghe
ONT is the *only* sequencing technology that detects RNA modifications directly. They're now up to 8 modifications that can be detected simultaneously.
[This is something I think ONT should be shouting from the rooftops every day]
RNA barcoding is in beta testing.
ONT is the *only* sequencing technology that detects RNA modifications directly. They're now up to 8 modifications that can be detected simultaneously.
[This is something I think ONT should be shouting from the rooftops every day]
RNA barcoding is in beta testing.
May 22, 2025 at 9:57 AM
#NanoporeConf Lakmal Jayasinghe
ONT is the *only* sequencing technology that detects RNA modifications directly. They're now up to 8 modifications that can be detected simultaneously.
[This is something I think ONT should be shouting from the rooftops every day]
RNA barcoding is in beta testing.
ONT is the *only* sequencing technology that detects RNA modifications directly. They're now up to 8 modifications that can be detected simultaneously.
[This is something I think ONT should be shouting from the rooftops every day]
RNA barcoding is in beta testing.
Reposted by Adrien Leger
New HAC models are 1.4Q more accurate - a 25% error reduction
May 22, 2025 at 11:39 AM
New HAC models are 1.4Q more accurate - a 25% error reduction
Reposted by Adrien Leger
Big improvements in modification calling accuracy - 6mA increases from Q16 to Q24
May 22, 2025 at 11:40 AM
Big improvements in modification calling accuracy - 6mA increases from Q16 to Q24
Reposted by Adrien Leger
Dorado v1.0.0 introduces dramatic improvement to modified base calling speed
May 22, 2025 at 11:40 AM
Dorado v1.0.0 introduces dramatic improvement to modified base calling speed
Reposted by Adrien Leger
Base calling obeys scaling laws in deep learning - a Q28 base caller using standard chemistry is now available on demand.
May 22, 2025 at 11:43 AM
Base calling obeys scaling laws in deep learning - a Q28 base caller using standard chemistry is now available on demand.
Reposted by Adrien Leger
Oxford Nanopore sequencing will be ubiquitous for multiomics….and the next step is proteomics.
First panel-based proteomics assays, before driving towards full protein sensing in the future. #nanoporeconf
First panel-based proteomics assays, before driving towards full protein sensing in the future. #nanoporeconf
May 21, 2025 at 6:27 PM
Oxford Nanopore sequencing will be ubiquitous for multiomics….and the next step is proteomics.
First panel-based proteomics assays, before driving towards full protein sensing in the future. #nanoporeconf
First panel-based proteomics assays, before driving towards full protein sensing in the future. #nanoporeconf
Reposted by Adrien Leger
More details on new RNA modified bases detectable with @nanoporetech.com sequencing. #NanoporeConf
May 21, 2025 at 6:20 PM
More details on new RNA modified bases detectable with @nanoporetech.com sequencing. #NanoporeConf
Reposted by Adrien Leger
Dorado 1.0 🚀release with new RNA modified base models for 2’Ome as well as improvement of all DNA and RNA modified base models including massive improvement in 6mA for open chromatin prediction! Also a lot more non-mods related features😆! #NanoporeConf
github.com/nanoporetech...
github.com/nanoporetech...
May 21, 2025 at 5:53 PM
Dorado 1.0 🚀release with new RNA modified base models for 2’Ome as well as improvement of all DNA and RNA modified base models including massive improvement in 6mA for open chromatin prediction! Also a lot more non-mods related features😆! #NanoporeConf
github.com/nanoporetech...
github.com/nanoporetech...
Reposted by Adrien Leger
Dorado 1.0 🚀release with new RNA modified base models for 2’Ome as well as improvement of all DNA and RNA modified base models including massive improvement in 6mA for open chromatin prediction! Also a lot more non-mods related features😆! #NanoporeConf
github.com/nanoporetech...
github.com/nanoporetech...
May 21, 2025 at 5:54 PM
Dorado 1.0 🚀release with new RNA modified base models for 2’Ome as well as improvement of all DNA and RNA modified base models including massive improvement in 6mA for open chromatin prediction! Also a lot more non-mods related features😆! #NanoporeConf
github.com/nanoporetech...
github.com/nanoporetech...
Reposted by Adrien Leger
With significant ML advances, Dorado & MinKNOW now power built-in accurate variant calling, default methylation detection & short fragment mode. Adaptive sampling & T2T assemblies are production-ready — enabling faster, richer, more meaningful insights.
May 21, 2025 at 5:38 PM
With significant ML advances, Dorado & MinKNOW now power built-in accurate variant calling, default methylation detection & short fragment mode. Adaptive sampling & T2T assemblies are production-ready — enabling faster, richer, more meaningful insights.
Reposted by Adrien Leger
Dorado is 1.0! 🎉
LC25 release adds v5.2 DNA/RNA models with a new suite of mod models that are higher accuracy, lower FP rates, and significantly faster.
- Introducing dorado variant 👀
- HAC error rate is down over 25%.
- New 2’Ome mod models
- Hopper & Blackwell SUP speed ups
#Nanoporeconf
LC25 release adds v5.2 DNA/RNA models with a new suite of mod models that are higher accuracy, lower FP rates, and significantly faster.
- Introducing dorado variant 👀
- HAC error rate is down over 25%.
- New 2’Ome mod models
- Hopper & Blackwell SUP speed ups
#Nanoporeconf
May 21, 2025 at 5:14 PM
Dorado is 1.0! 🎉
LC25 release adds v5.2 DNA/RNA models with a new suite of mod models that are higher accuracy, lower FP rates, and significantly faster.
- Introducing dorado variant 👀
- HAC error rate is down over 25%.
- New 2’Ome mod models
- Hopper & Blackwell SUP speed ups
#Nanoporeconf
LC25 release adds v5.2 DNA/RNA models with a new suite of mod models that are higher accuracy, lower FP rates, and significantly faster.
- Introducing dorado variant 👀
- HAC error rate is down over 25%.
- New 2’Ome mod models
- Hopper & Blackwell SUP speed ups
#Nanoporeconf
Reposted by Adrien Leger
Your speakers for London Calling 2025! #NanoporeConf
May 21, 2025 at 10:00 AM
Your speakers for London Calling 2025! #NanoporeConf
Reposted by Adrien Leger
Really pleased that this paper is out - academic.oup.com/neuro-oncolo... thanks to all our collaborators far and wide who have helped and of course @nanoporetech.com
ROBIN: A unified nanopore-based assay integrating intraoperative methylome classification and next-day comprehensive profiling for ultra-rapid tumor diagnosis
academic.oup.com
May 21, 2025 at 9:26 AM
Really pleased that this paper is out - academic.oup.com/neuro-oncolo... thanks to all our collaborators far and wide who have helped and of course @nanoporetech.com
Reposted by Adrien Leger
Excited for another London Calling! Incredible updates coming over the next two days! #NanoporeConf @nanoporetech.com
May 21, 2025 at 8:59 AM
Excited for another London Calling! Incredible updates coming over the next two days! #NanoporeConf @nanoporetech.com
Reposted by Adrien Leger
I am quite interesting in this Nm modification. As we detected Nm in rRNA as well as mRNA using Nanopore RNA002 version. www.cell.com/molecular-ce...
2′-O-methylation at internal sites on mRNA promotes mRNA stability
Li et al. develop a machine learning approach to measure the stoichiometry of 2′-O-methylation
at internal sites of mRNA using nanopore RNA sequencing data. FBL-mediated Nm modifications,
when connect...
www.cell.com
February 21, 2025 at 9:16 PM
I am quite interesting in this Nm modification. As we detected Nm in rRNA as well as mRNA using Nanopore RNA002 version. www.cell.com/molecular-ce...
Reposted by Adrien Leger
I expect Rosemary and Lakmal (the new R&D team leads) will reveal more than just all this - I am looking forward to the reports on Wednesday and looking forward to catching up with everyone nanopore on Friday!
May 20, 2025 at 8:04 AM
I expect Rosemary and Lakmal (the new R&D team leads) will reveal more than just all this - I am looking forward to the reports on Wednesday and looking forward to catching up with everyone nanopore on Friday!
Reposted by Adrien Leger
Nanopore now can call 4 RNA modifications - m6A (I love how DNA and RNA have different writing conventions - molecular biologists, just don't go changing!) - m5C, pseudourodine, and inosine. This is all new stuff, and there quite a bit on truth sets, sensitivity/specificity to play out
May 20, 2025 at 8:04 AM
Nanopore now can call 4 RNA modifications - m6A (I love how DNA and RNA have different writing conventions - molecular biologists, just don't go changing!) - m5C, pseudourodine, and inosine. This is all new stuff, and there quite a bit on truth sets, sensitivity/specificity to play out
Reposted by Adrien Leger
The final, most exotic stuff is RNA - so called "direct RNA" where modifications of the RNA is the really exciting stuff (the sequence itself, including intron/exon structure is probably still done best via cDNA, "old school").
May 20, 2025 at 8:04 AM
The final, most exotic stuff is RNA - so called "direct RNA" where modifications of the RNA is the really exciting stuff (the sequence itself, including intron/exon structure is probably still done best via cDNA, "old school").
Reposted by Adrien Leger
Basically molecular biologists can either use incorporation (eg, BrdU) or modification (eg, bacterial enzymes tethered to antibodies) or chemical modification (eg, radicals etc) and then the read out is the DNA (or RNA, see below).
May 20, 2025 at 8:04 AM
Basically molecular biologists can either use incorporation (eg, BrdU) or modification (eg, bacterial enzymes tethered to antibodies) or chemical modification (eg, radicals etc) and then the read out is the DNA (or RNA, see below).