Adrien Leger
@adrienleger.bsky.social
Director of Modified Bases Research at @nanoporetech.com
EMBL-EBI alumni, keen cyclist and father of two.
Views are my own. Reposts are not endorsement.
EMBL-EBI alumni, keen cyclist and father of two.
Views are my own. Reposts are not endorsement.
Reposted by Adrien Leger
More details on new RNA modified bases detectable with @nanoporetech.com sequencing. #NanoporeConf
May 21, 2025 at 6:20 PM
More details on new RNA modified bases detectable with @nanoporetech.com sequencing. #NanoporeConf
Reposted by Adrien Leger
I am quite interesting in this Nm modification. As we detected Nm in rRNA as well as mRNA using Nanopore RNA002 version. www.cell.com/molecular-ce...
2′-O-methylation at internal sites on mRNA promotes mRNA stability
Li et al. develop a machine learning approach to measure the stoichiometry of 2′-O-methylation
at internal sites of mRNA using nanopore RNA sequencing data. FBL-mediated Nm modifications,
when connect...
www.cell.com
February 21, 2025 at 9:16 PM
I am quite interesting in this Nm modification. As we detected Nm in rRNA as well as mRNA using Nanopore RNA002 version. www.cell.com/molecular-ce...
Reposted by Adrien Leger
I expect Rosemary and Lakmal (the new R&D team leads) will reveal more than just all this - I am looking forward to the reports on Wednesday and looking forward to catching up with everyone nanopore on Friday!
May 20, 2025 at 8:04 AM
I expect Rosemary and Lakmal (the new R&D team leads) will reveal more than just all this - I am looking forward to the reports on Wednesday and looking forward to catching up with everyone nanopore on Friday!
Reposted by Adrien Leger
Nanopore now can call 4 RNA modifications - m6A (I love how DNA and RNA have different writing conventions - molecular biologists, just don't go changing!) - m5C, pseudourodine, and inosine. This is all new stuff, and there quite a bit on truth sets, sensitivity/specificity to play out
May 20, 2025 at 8:04 AM
Nanopore now can call 4 RNA modifications - m6A (I love how DNA and RNA have different writing conventions - molecular biologists, just don't go changing!) - m5C, pseudourodine, and inosine. This is all new stuff, and there quite a bit on truth sets, sensitivity/specificity to play out
Reposted by Adrien Leger
The final, most exotic stuff is RNA - so called "direct RNA" where modifications of the RNA is the really exciting stuff (the sequence itself, including intron/exon structure is probably still done best via cDNA, "old school").
May 20, 2025 at 8:04 AM
The final, most exotic stuff is RNA - so called "direct RNA" where modifications of the RNA is the really exciting stuff (the sequence itself, including intron/exon structure is probably still done best via cDNA, "old school").
Reposted by Adrien Leger
Basically molecular biologists can either use incorporation (eg, BrdU) or modification (eg, bacterial enzymes tethered to antibodies) or chemical modification (eg, radicals etc) and then the read out is the DNA (or RNA, see below).
May 20, 2025 at 8:04 AM
Basically molecular biologists can either use incorporation (eg, BrdU) or modification (eg, bacterial enzymes tethered to antibodies) or chemical modification (eg, radicals etc) and then the read out is the DNA (or RNA, see below).