#NanoporeConf Lakmal Jayasinghe

ONT is the *only* sequencing technology that detects RNA modifications directly. They're now up to 8 modifications that can be detected simultaneously.

[This is something I think ONT should be shouting from the rooftops every day]

RNA barcoding is in beta testing.

Oxford Nanopore sequencing will be ubiquitous for multiomics….and the next step is proteomics.

First panel-based proteomics assays, before driving towards full protein sensing in the future. #nanoporeconf

The future of @nanoporetech.com sequencing is here now! #NanoporeConf #Proteomics

Amazing time to be working with @nanoporetech.com sequencing! The pace of innovation in this network of researchers is astonishing! #NanoporeConf

Dorado 1.0 🚀release with new RNA modified base models for 2’Ome as well as improvement of all DNA and RNA modified base models including massive improvement in 6mA for open chromatin prediction! Also a lot more non-mods related features😆! #NanoporeConf

github.com/nanoporetech...

Dorado 1.0 🚀release with new RNA modified base models for 2’Ome as well as improvement of all DNA and RNA modified base models including massive improvement in 6mA for open chromatin prediction! Also a lot more non-mods related features😆! #NanoporeConf

github.com/nanoporetech...

Initial release of Pomfret for methylation assisted phase block joining for more contiguous phased assemblies without any additional library prep from @nanoporetech.com data. Such amazing work from Xiaowen Feng! #NanoporeConf

github.com/nanoporetech...

Exciting modkit release with open chromatin prediction support and more! Amazing work from Art Rand! @nanoporetech.com #NanoporeConf

github.com/nanoporetech...

Methylation assists tandem repeat expansion estimation from @nanoporetech.com sequencing using strcount. #NanoporeConf

Really pleased that this paper is out - academic.oup.com/neuro-oncolo... thanks to all our collaborators far and wide who have helped and of course @nanoporetech.com

Excited for another London Calling! Incredible updates coming over the next two days! #NanoporeConf @nanoporetech.com

RNA xkcd.com/3056

An overview of nanopore sequencing for pangenomes and hemi-hydroxymethylation in medaka fish brains by @ewanbirney.bsky.social
Presenting results showing biased 5hmC/5mC hemimethylation at splice sites, and increased skewed hemimethylation across repetitive sequences.

Since Oxford Nanopore sequencing does not require any chemical (bisulfite) conversion to detect methylation. It would mean long-range epigenetic modifications, structural variants (SVs),single nucleotide polymorphisms (SNPs), and repeats can all be identified and phased in a single dataset

We are proud to announce a collaboration with UK Biobank to create the world’s first large-scale #epigenetic dataset of 50k participants. The dataset will unlock crucial insights into how #epigenetics drives disease & the breakthroughs to treat them.

Read more: nanoporetech.com/news/oxford-...

Thank u for this question. Worth another thread as it reflects common confusion that more data & better AI/ML is the solution (newer official @nanopore models & new customer methods are being developed/released). For abundant mods like 5mCpG in human, this strategy works. *BUT* for low abundance 1/5

Long-read special issue of GR is out, including our paper on T2T assembly using only Nanopore. Just in time for ONT to discontinue duplex cells on Nov 27 😅😂 Good thing Verkko also works great with HERRO-corrected simplex data!
📄 genome.cshlp.org/content/34/1...
📖 genome.cshlp.org/content/34/1...