Friends don’t let friends identify glycopeptides by deamidation. Even non-glyco proteomics people know that deamidation can be an artifact.

Either intact or endoglycosidase (see Yates lab DeGlypher approach) is best.

Do you or your lab use the Skyline software for #MassSpectrometry #proteomics? I'm looking for instructors to help with this year's Skyline Online, a virtual workshop/crash-course for all things Skyline! I'm especially looking for early career researchers for this opportunity. Please DM!

N-Glycopedia: Libraries for Native N-glycan Structural Analysis https://www.biorxiv.org/content/10.1101/2025.06.09.658590v1

By deep characterisation of 186 N-glycan standards, we @thencfg.bsky.social have made the most comprehensive targeted LC-MS glycomic assay to date.
Non-reduced native glycan analysis is key.
We hope this enables anyone with QqQ and interest in #glycotime to measure specific N-glycan structures.

Mind Your Spectra: Points to be Aware of When Validating the Identification of Isobaric Histone Peptidoforms pubs.acs.org/doi/10....

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#proteomics #prot-paper

“I insist on being shocked. I am never going to become immune. I think that’s a kind of failure to see so much of it that you die inside. I want to be surprised and shocked every time.”
― Toni Morrison

My mentor and dear friend @roxanegay.bsky.social recently joined me here at Harvard Medical School for an incredible event. We both read new work and then had a wide-ranging discussion about the creative life, politics and culture.

I’m delighted to be able to share the video now:

I love a book where failure to calibrate instruments is part of the plot! People play fast and loose with Western blots - please use mass spec. A fast and informative read - and at the end you realize how much science is driven by repetitional hierarchy not research quality.

Locating Polyubiquitin Receptors on the 19S Regulatory Proteasome of S. cerevisiae by Cross-Linking Mass Spectrometry #JASMS pubs.acs.org/doi/10.1021/...

Multi-Reflecting TOF MS for Analyzing Proteins #IJMS www.sciencedirect.com/science/arti...

Who would have thought that #dolphins (short-finned pilot whale, killer whale, false killer whale and pantropical spotted dolphin) host #anelloviruses with the most complex, extravagantly large capsid proteins? Collab. with the Varsani (@viralnomad.bsky.social) team journals.asm.org/doi/10.1128/...

My MS predictions for 2025:
- ⬆️Single cell applications and extending linearity over a wide dynamic range (Bruker - new timsTOF, Thermo - new Exploris)
- TMT32 and 35-plex better showcased
- Thermo OptiSpray dual column-emitter source
- Thermo isotope ratio Exploris release
#ASMS2025 #TeamMassSpec

🎉 Celebrating 20 incredible years of US HUPO🎉 As 2024 comes to a close, we are excited to share reflections from our wonderful members about the advancements and memorable moments in #Proteomics over the past two decades. ✨Check out the poster on our website: ushupo.org/20-years/ #USHUPO20YRS

“Wrong does not cease to be wrong because the majority share in it.”

— Leo Tolstoy (A Confession)