Volker Boehm
@volkerboehm.bsky.social
RNA-centric Staff Scientist | Nonsense-mediated decay | Exon junction complex | RBPs | Gehring Lab @ University of Cologne 🧫 https://orcid.org/0000-0001-7588-9842
Reposted by Volker Boehm
🚨Speaker Alert 🚨
We’re excited to welcome @landthalerm.bsky.social for the upcoming #CRC1678 Seminar!
🧬 He’ll dive into: "Posttranscriptional Regulation in Cellular Time and Space"
📅 November 25
📍 Auditorium, MPI-Age
⏰ 2:00 PM
Explore how RNA dynamics shape cellular behavior across time and space!
We’re excited to welcome @landthalerm.bsky.social for the upcoming #CRC1678 Seminar!
🧬 He’ll dive into: "Posttranscriptional Regulation in Cellular Time and Space"
📅 November 25
📍 Auditorium, MPI-Age
⏰ 2:00 PM
Explore how RNA dynamics shape cellular behavior across time and space!
November 14, 2025 at 1:25 PM
🚨Speaker Alert 🚨
We’re excited to welcome @landthalerm.bsky.social for the upcoming #CRC1678 Seminar!
🧬 He’ll dive into: "Posttranscriptional Regulation in Cellular Time and Space"
📅 November 25
📍 Auditorium, MPI-Age
⏰ 2:00 PM
Explore how RNA dynamics shape cellular behavior across time and space!
We’re excited to welcome @landthalerm.bsky.social for the upcoming #CRC1678 Seminar!
🧬 He’ll dive into: "Posttranscriptional Regulation in Cellular Time and Space"
📅 November 25
📍 Auditorium, MPI-Age
⏰ 2:00 PM
Explore how RNA dynamics shape cellular behavior across time and space!
Reposted by Volker Boehm
Happy to see this work published! Thanks to everyone who contributed 🙏
Publication by the Mühlemann & Chakrabarti Labs w/ 1st author Sofia Nasif "UPF1 shuttles between nucleus and cytoplasm independently of its RNA binding and ATPase activities” @rnajournal.bsky.social
nccr-rna-and-disease.ch/news/article...
@omuhlemann-lab.bsky.social
@unibe.ch
nccr-rna-and-disease.ch/news/article...
@omuhlemann-lab.bsky.social
@unibe.ch
October 13, 2025 at 8:11 AM
Happy to see this work published! Thanks to everyone who contributed 🙏
Reposted by Volker Boehm
Dusa’s study is on the cover of this month’s #CellGenomics! Check it out if you haven’t had the chance yet!
www.cell.com/issue/S2666-...
www.cell.com/issue/S2666-...
October 10, 2025 at 9:32 AM
Dusa’s study is on the cover of this month’s #CellGenomics! Check it out if you haven’t had the chance yet!
www.cell.com/issue/S2666-...
www.cell.com/issue/S2666-...
Reposted by Volker Boehm
🛑Guille's @guillepalou.bsky.social no-nonsense 😉 paper is out in @springernature.com Genome Biology!
We report variable activity of the NMD pathway (mRNA QC) across tissues & individuals:
🧠less active in the nervous system & in reproductive tissues
🩸more active in digestive tract, muscle, blood
We report variable activity of the NMD pathway (mRNA QC) across tissues & individuals:
🧠less active in the nervous system & in reproductive tissues
🩸more active in digestive tract, muscle, blood
September 29, 2025 at 4:54 PM
🛑Guille's @guillepalou.bsky.social no-nonsense 😉 paper is out in @springernature.com Genome Biology!
We report variable activity of the NMD pathway (mRNA QC) across tissues & individuals:
🧠less active in the nervous system & in reproductive tissues
🩸more active in digestive tract, muscle, blood
We report variable activity of the NMD pathway (mRNA QC) across tissues & individuals:
🧠less active in the nervous system & in reproductive tissues
🩸more active in digestive tract, muscle, blood
Reposted by Volker Boehm
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
September 22, 2025 at 6:27 PM
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Reposted by Volker Boehm
wrong bioRxiv link here, sorry.
the correct link to the collaboration with Torben and Clemens is:
www.biorxiv.org/content/10.1...
the correct link to the collaboration with Torben and Clemens is:
www.biorxiv.org/content/10.1...
September 17, 2025 at 4:55 PM
wrong bioRxiv link here, sorry.
the correct link to the collaboration with Torben and Clemens is:
www.biorxiv.org/content/10.1...
the correct link to the collaboration with Torben and Clemens is:
www.biorxiv.org/content/10.1...
Reposted by Volker Boehm
The team of Prof. Dr Niels Gehring @volkerboehm.bsky.social records a comprehensive database that records systematically which genes and gene variants are directly affected by "Nonsense-mediated mRNA Decay" which opens up new avenues for RNA and genome research.
The database ➡️ nmdrht.uni-koeln.de
The database ➡️ nmdrht.uni-koeln.de
NMDRHT v1.2
nmdrht.uni-koeln.de
September 12, 2025 at 12:51 PM
The team of Prof. Dr Niels Gehring @volkerboehm.bsky.social records a comprehensive database that records systematically which genes and gene variants are directly affected by "Nonsense-mediated mRNA Decay" which opens up new avenues for RNA and genome research.
The database ➡️ nmdrht.uni-koeln.de
The database ➡️ nmdrht.uni-koeln.de
Reposted by Volker Boehm
🥳 Congratulations from #CRC1678 to @volkerboehm.bsky.social and the Gehring lab for their publication in @cp-molcell.bsky.social on how “Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome” 🧬🧫
🧐 This is how quality control works in our cells 🧬
Researchers from the #unicologne and the @mdc-berlin.bsky.social have cracked the code of a crucial mechanism of cellular quality control in humans. 👇
uni.koeln/RRCLZ
Researchers from the #unicologne and the @mdc-berlin.bsky.social have cracked the code of a crucial mechanism of cellular quality control in humans. 👇
uni.koeln/RRCLZ
Cellular quality control in humans decoded
A research team at the University of Cologne and the Max Delbrück Center in Berlin has deciphered in detail the rules behind an important mechanism of cellular quality control in humans / Publication ...
uni.koeln
September 15, 2025 at 4:12 PM
🥳 Congratulations from #CRC1678 to @volkerboehm.bsky.social and the Gehring lab for their publication in @cp-molcell.bsky.social on how “Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome” 🧬🧫
Reposted by Volker Boehm
Very harmonious and inspiring collaboration with Niels Gehring, @volkerboehm.bsky.social and team at Uni Köln, and colleagues at @mdc-berlin.bsky.social . Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome: Molecular Cell www.cell.com/molecular-ce...
Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome
UPF1 depletion inhibits nonsense-mediated mRNA decay (NMD) and stabilizes direct target
transcripts within hours. Boehm et al. use conditional protein degradation and advanced
transcriptomics to map t...
www.cell.com
September 12, 2025 at 7:26 AM
Very harmonious and inspiring collaboration with Niels Gehring, @volkerboehm.bsky.social and team at Uni Köln, and colleagues at @mdc-berlin.bsky.social . Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome: Molecular Cell www.cell.com/molecular-ce...
Reposted by Volker Boehm
🎉 Congratulations to our new Vice-Rector for Research, Professor Dr. Carien Niessen! 🙌
The University Council has elected Professor Niessen as Vice-Rector for Research to support the advancement of excellent research at the #UniCologne. 🎓
Read more 👇
uni.koeln/9FCEC
@cecad.bsky.social
The University Council has elected Professor Niessen as Vice-Rector for Research to support the advancement of excellent research at the #UniCologne. 🎓
Read more 👇
uni.koeln/9FCEC
@cecad.bsky.social
Carien Niessen elected new Vice-Rector for Research at the University of Cologne
The University Election Assembly elected Professor Dr Carien Niessen for the office of Vice-Rector for Research. The cell biologist is particularly committed to promoting excellent research at the uni...
uni.koeln
September 10, 2025 at 10:34 AM
🎉 Congratulations to our new Vice-Rector for Research, Professor Dr. Carien Niessen! 🙌
The University Council has elected Professor Niessen as Vice-Rector for Research to support the advancement of excellent research at the #UniCologne. 🎓
Read more 👇
uni.koeln/9FCEC
@cecad.bsky.social
The University Council has elected Professor Niessen as Vice-Rector for Research to support the advancement of excellent research at the #UniCologne. 🎓
Read more 👇
uni.koeln/9FCEC
@cecad.bsky.social
Reposted by Volker Boehm
Online Now: Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome Online now:
Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome
UPF1 depletion inhibits nonsense-mediated mRNA decay (NMD) and stabilizes direct target transcripts within hours. Boehm et al. use conditional protein degradation and advanced transcriptomics to map the NMD-regulated human transcriptome, highlighting alternative splicing as a major NMD-activating mechanism.
dlvr.it
September 10, 2025 at 3:19 PM
Online Now: Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome Online now:
Reposted by Volker Boehm
Many conserved exons in the heart and brain utilize weak 5 ′ splice sites, yet they are accurately spliced. But how? We show that splicing fidelity is actively enforced through a QKI-U6 checkpoint at the U1→U6 handover in essential cardiac genes during organogenesis.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
QKI ensures splicing fidelity during cardiogenesis by engaging the U6 tri-snRNP to activate splicing at weak 5ʹ splice sites
During organogenesis, precise pre-mRNA splicing is essential to assemble tissue architecture. Many developmentally essential exons bear weak 5'splice sites (5'SS) yet are spliced with high precision, ...
www.biorxiv.org
September 8, 2025 at 6:12 AM
Many conserved exons in the heart and brain utilize weak 5 ′ splice sites, yet they are accurately spliced. But how? We show that splicing fidelity is actively enforced through a QKI-U6 checkpoint at the U1→U6 handover in essential cardiac genes during organogenesis.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Volker Boehm
"m6A in the coding sequence: linking deposition, translation and decay"
by Kathi Zarnack (@zarnack-group.bsky.social) & colleagues
"This review explores [m6A in] mRNA decay, with a focus on the newly discovered CDS–m6A decay (CMD) pathway..."
Read it here:
authors.elsevier.com/sd/article/S...
by Kathi Zarnack (@zarnack-group.bsky.social) & colleagues
"This review explores [m6A in] mRNA decay, with a focus on the newly discovered CDS–m6A decay (CMD) pathway..."
Read it here:
authors.elsevier.com/sd/article/S...
July 8, 2025 at 12:07 PM
"m6A in the coding sequence: linking deposition, translation and decay"
by Kathi Zarnack (@zarnack-group.bsky.social) & colleagues
"This review explores [m6A in] mRNA decay, with a focus on the newly discovered CDS–m6A decay (CMD) pathway..."
Read it here:
authors.elsevier.com/sd/article/S...
by Kathi Zarnack (@zarnack-group.bsky.social) & colleagues
"This review explores [m6A in] mRNA decay, with a focus on the newly discovered CDS–m6A decay (CMD) pathway..."
Read it here:
authors.elsevier.com/sd/article/S...
Reposted by Volker Boehm
The latest human GENCODE releases for Human (49) and Mouse (M38), have just been released. They're available via gencodegenes.org and visible in @ensembl genome browser as part of Ensembl 115 ensembl.org
GENCODE - Home page
gencodegenes.org
September 3, 2025 at 11:16 AM
The latest human GENCODE releases for Human (49) and Mouse (M38), have just been released. They're available via gencodegenes.org and visible in @ensembl genome browser as part of Ensembl 115 ensembl.org
Reposted by Volker Boehm
Our study from the Steckelberg lab is out now in @narjournal.bsky.social !! We dove into a new nuclease-blocking viral #RNA structure important for viral infection, which provided evidence of similar structure-based strategies across diverse viral families. academic.oup.com/nar/article/...
A conserved viral RNA fold enables nuclease resistance across kingdoms of life
Abstract. Viral exoribonuclease-resistant RNA (xrRNA) structures block cellular nucleases to produce subgenomic viral RNAs during infection. High sequence
academic.oup.com
September 2, 2025 at 6:58 PM
Our study from the Steckelberg lab is out now in @narjournal.bsky.social !! We dove into a new nuclease-blocking viral #RNA structure important for viral infection, which provided evidence of similar structure-based strategies across diverse viral families. academic.oup.com/nar/article/...
Reposted by Volker Boehm
Delighted to present our second paper of the year. This one explores the molecular mechanism of TTP, a key post-transcriptional regulator of AU-rich mRNAs. Work led and coordinated by @filippekovic.bsky.social, in collaboration with Perry Blackshear.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Multivalent interactions with CCR4–NOT and PABPC1 determine mRNA repression efficiency by tristetraprolin - Nature Communications
Deadenylation leads to mRNA decay, with PABPC1 protecting the poly(A) tail, while tristetraprolin and CCR4–NOT promote deadenylation. Here, the authors describe how these three proteins interact to re...
www.nature.com
August 13, 2025 at 5:32 PM
Delighted to present our second paper of the year. This one explores the molecular mechanism of TTP, a key post-transcriptional regulator of AU-rich mRNAs. Work led and coordinated by @filippekovic.bsky.social, in collaboration with Perry Blackshear.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Volker Boehm
In bulk RNA-seq, @plbaldoni.bsky.social et al showed that correcting overdispersion (OD) from read-to-transcript mapping ambiguity improves downstream analyses. Here I extend this to scRNA-seq using @mikelove.bsky.social et al Alevin’s bootstraps to correct per-cell OD github.com/sbresnahan/O...
GitHub - sbresnahan/Overdispersion-Correction-for-Alevin: Processing Salmon Alevin bootstrap replicates to account for technical uncertainty before integrating single-cell RNA-seq datasets in Seurat
Processing Salmon Alevin bootstrap replicates to account for technical uncertainty before integrating single-cell RNA-seq datasets in Seurat - sbresnahan/Overdispersion-Correction-for-Alevin
github.com
August 9, 2025 at 1:52 PM
In bulk RNA-seq, @plbaldoni.bsky.social et al showed that correcting overdispersion (OD) from read-to-transcript mapping ambiguity improves downstream analyses. Here I extend this to scRNA-seq using @mikelove.bsky.social et al Alevin’s bootstraps to correct per-cell OD github.com/sbresnahan/O...
Reposted by Volker Boehm
Final call for postdoc positions in Aarhus, Denmark. Sorting of gene expression and beyond. Please get in touch for further information or apply using this link:
mbg.au.dk/en/news-and-...
mbg.au.dk/en/news-and-...
A postdoc position in the Torben Heick Jensen lab, Aarhus University, Denmark: Mammalian Nuclear RNA Production and Turnover Systems - Vacancy at Aarhus University
Vacancy at Department of Molecular Biology and Genetics - RNA Biology and Innovation, Aarhus University
mbg.au.dk
August 5, 2025 at 8:06 AM
Final call for postdoc positions in Aarhus, Denmark. Sorting of gene expression and beyond. Please get in touch for further information or apply using this link:
mbg.au.dk/en/news-and-...
mbg.au.dk/en/news-and-...
Reposted by Volker Boehm
Excited for this to be out officially! It was a great team effort and has a lot of useful tidbits for studying isoform function. www.nature.com/articles/s41...
Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolbox - Nature Communications
The majority of human genes can produce multiple isoforms, but studying their functional relevance requires tools to target specific isoforms. Here, the authors develop a CRISPR-based exon-exon juncti...
www.nature.com
July 29, 2025 at 4:35 PM
Excited for this to be out officially! It was a great team effort and has a lot of useful tidbits for studying isoform function. www.nature.com/articles/s41...
Reposted by Volker Boehm
🚨🚨🚨
Please RT!
We're looking for a postdoc to join an exciting joint project between our lab @upf.edu & @crg.eu (Barcelona) and the Sander lab @mdc-berlin.bsky.social (Berlin) investigating how alternative splicing and microexons influences the maturation of pancreatic islets.
Deadline: 30/09/25👇
Please RT!
We're looking for a postdoc to join an exciting joint project between our lab @upf.edu & @crg.eu (Barcelona) and the Sander lab @mdc-berlin.bsky.social (Berlin) investigating how alternative splicing and microexons influences the maturation of pancreatic islets.
Deadline: 30/09/25👇
www.upf.edu
July 23, 2025 at 2:28 PM
🚨🚨🚨
Please RT!
We're looking for a postdoc to join an exciting joint project between our lab @upf.edu & @crg.eu (Barcelona) and the Sander lab @mdc-berlin.bsky.social (Berlin) investigating how alternative splicing and microexons influences the maturation of pancreatic islets.
Deadline: 30/09/25👇
Please RT!
We're looking for a postdoc to join an exciting joint project between our lab @upf.edu & @crg.eu (Barcelona) and the Sander lab @mdc-berlin.bsky.social (Berlin) investigating how alternative splicing and microexons influences the maturation of pancreatic islets.
Deadline: 30/09/25👇
Reposted by Volker Boehm
1/ 🧬 Exciting news! Our group just published a paper in Nature Communications on using serum proteomics to predict disease progression in #ADPKD (Autosomal Dominant Polycystic Kidney Disease) 🔬 — enhancing risk stratification beyond currently available models 🎯 rdcu.be/exbNq
Developing serum proteomics based prediction models of disease progression in ADPKD
Nature Communications - Autosomal dominant polycystic kidney disease (ADPKD) is characterized by progressive cyst formation and loss of kidney function, yet prognostic biomarkers remain limited....
rdcu.be
July 21, 2025 at 4:13 PM
1/ 🧬 Exciting news! Our group just published a paper in Nature Communications on using serum proteomics to predict disease progression in #ADPKD (Autosomal Dominant Polycystic Kidney Disease) 🔬 — enhancing risk stratification beyond currently available models 🎯 rdcu.be/exbNq
Reposted by Volker Boehm
Hi #RNAsky, #ChromatinSky!
It is a pleasure to announce that the @ox.ac.uk RNA & transcription club is now on Bluesky. Follow us to stay in touch with everything RNA and transcription from gene regulation to disease. Join us here: rna.web.ox.ac.uk/home
It is a pleasure to announce that the @ox.ac.uk RNA & transcription club is now on Bluesky. Follow us to stay in touch with everything RNA and transcription from gene regulation to disease. Join us here: rna.web.ox.ac.uk/home
Home
rna.web.ox.ac.uk
July 18, 2025 at 9:47 AM
Hi #RNAsky, #ChromatinSky!
It is a pleasure to announce that the @ox.ac.uk RNA & transcription club is now on Bluesky. Follow us to stay in touch with everything RNA and transcription from gene regulation to disease. Join us here: rna.web.ox.ac.uk/home
It is a pleasure to announce that the @ox.ac.uk RNA & transcription club is now on Bluesky. Follow us to stay in touch with everything RNA and transcription from gene regulation to disease. Join us here: rna.web.ox.ac.uk/home
Reposted by Volker Boehm
Happy to share the latest work on a bacterial protein that inhibits human mRNA decay machinery, in collaboration with @smlea.bsky.social #RNAsky
www.nature.com/articles/s41... Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay | Nature Communications
www.nature.com/articles/s41... Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay | Nature Communications
Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay - Nature Communications
Intracellular pathogens hijack host gene expression to subvert cellular processes. Here, the authors show that Legionella pneumophila’s PieF effector inhibits the human CCR4-NOT deadenylation machiner...
www.nature.com
July 13, 2025 at 3:59 AM
Happy to share the latest work on a bacterial protein that inhibits human mRNA decay machinery, in collaboration with @smlea.bsky.social #RNAsky
www.nature.com/articles/s41... Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay | Nature Communications
www.nature.com/articles/s41... Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay | Nature Communications
Reposted by Volker Boehm
Freshly launched! 🚀 Check out the new CRC 1678 website 🌐
👉 crc1678.uni-koeln.de
👉 crc1678.uni-koeln.de
About CRC 1678 - CRC1678 - Collaborative Research Center
CRC 1678 investigates how age and stress-driven declines in the accuracy of mRNA and protein production affect cellular and organismal health. By taking an
crc1678.uni-koeln.de
July 11, 2025 at 3:48 PM
Freshly launched! 🚀 Check out the new CRC 1678 website 🌐
👉 crc1678.uni-koeln.de
👉 crc1678.uni-koeln.de
Reposted by Volker Boehm
Congratulations to our biochemistry professor Utz Fischer: he has been elected to @embo.org ! His research focuses on the macromolecular machines of the cell. 📷 Christoph Weiss www.uni-wuerzburg.de/en/news-and-...
July 1, 2025 at 12:03 PM
Congratulations to our biochemistry professor Utz Fischer: he has been elected to @embo.org ! His research focuses on the macromolecular machines of the cell. 📷 Christoph Weiss www.uni-wuerzburg.de/en/news-and-...