Sheila Teves
@sheilateves.bsky.social
Assistant Professor, Biochemistry Department, Transcription & Chromatin biology, Outdoor enthusiast, Dog lover (otters too) 🇵🇭🇺🇲🇨🇦
Pinned
Sheila Teves
@sheilateves.bsky.social
· Nov 8
Functional divergence of TBP homologs through distinct DNA binding dynamics
The TATA box-binding protein (TBP) is an evolutionarily conserved basal transcription factor common in the pre-initiation complex of all three eukaryotic RNA polymerases (RNA Pols). Despite their high...
biorxiv.org
Latest research from the Teves lab is now out on Biorxiv!
"Functional divergence of TBP homologs through distinct DNA binding dynamics" biorxiv.org/content/10.1...
Here, we examine how conservation is balanced with diversification throughout evolution, using TBP as a case study.
"Functional divergence of TBP homologs through distinct DNA binding dynamics" biorxiv.org/content/10.1...
Here, we examine how conservation is balanced with diversification throughout evolution, using TBP as a case study.
Reposted by Sheila Teves
What is a promoter? And how does it work?
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
Enhancer-promoter compatibility is mediated by the promoter-proximal region
Gene promoters induce transcription in response to distal enhancers. How enhancers specifically activate their target promoter while bypassing other promoters remains unclear. Here, we find that the p...
www.biorxiv.org
October 16, 2025 at 3:06 PM
What is a promoter? And how does it work?
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
How does a “universal” transcription factor evolve to do species-specific jobs?
Our new preprint reveals how divergence in TBP’s domains shapes transcriptional specialization across eukaryotes.
Read it here 👉
www.biorxiv.org/content/10.1...
#MolecularEvolution #Transcription
Our new preprint reveals how divergence in TBP’s domains shapes transcriptional specialization across eukaryotes.
Read it here 👉
www.biorxiv.org/content/10.1...
#MolecularEvolution #Transcription
Molecular determinants underlying functional divergence of TBP homologs
The TATA-box binding protein (TBP) is a highly conserved basal transcription factor and a core component of the pre-initiation complex (PIC) for all three eukaryotic RNA polymerases (RNA Pols). Despit...
www.biorxiv.org
October 7, 2025 at 7:43 PM
How does a “universal” transcription factor evolve to do species-specific jobs?
Our new preprint reveals how divergence in TBP’s domains shapes transcriptional specialization across eukaryotes.
Read it here 👉
www.biorxiv.org/content/10.1...
#MolecularEvolution #Transcription
Our new preprint reveals how divergence in TBP’s domains shapes transcriptional specialization across eukaryotes.
Read it here 👉
www.biorxiv.org/content/10.1...
#MolecularEvolution #Transcription
Flung some discs, shared some laughs, and capped it off with good food. Summer vibes from the Teves lab!
June 12, 2025 at 3:07 AM
Flung some discs, shared some laughs, and capped it off with good food. Summer vibes from the Teves lab!
Vancouver showing pride
June 5, 2025 at 1:41 AM
Vancouver showing pride
So excited to see this work out in
@narjournal.bsky.social
academic.oup.com/nar/article/...
Congratulations to Hazel Cui on outstanding work!
Keep an eye out for part 2 of this project 😎
@narjournal.bsky.social
academic.oup.com/nar/article/...
Congratulations to Hazel Cui on outstanding work!
Keep an eye out for part 2 of this project 😎
May 22, 2025 at 1:52 AM
So excited to see this work out in
@narjournal.bsky.social
academic.oup.com/nar/article/...
Congratulations to Hazel Cui on outstanding work!
Keep an eye out for part 2 of this project 😎
@narjournal.bsky.social
academic.oup.com/nar/article/...
Congratulations to Hazel Cui on outstanding work!
Keep an eye out for part 2 of this project 😎
Reposted by Sheila Teves
In #GENETICS, @sheilateves.bsky.social et al. study the regulation of Pol III transcription during different stages of heat shock stress in mouse embryonic stem cells, showing that the dynamic regulation & recovery of Pol III may be exclusive to the heat shock response.
Read more: buff.ly/s966iZ1
Read more: buff.ly/s966iZ1
May 7, 2025 at 10:02 PM
In #GENETICS, @sheilateves.bsky.social et al. study the regulation of Pol III transcription during different stages of heat shock stress in mouse embryonic stem cells, showing that the dynamic regulation & recovery of Pol III may be exclusive to the heat shock response.
Read more: buff.ly/s966iZ1
Read more: buff.ly/s966iZ1
So proud of Thomas Nguyen and co-authors on the publication of this manuscript. Now out in Genetics!
See full paper here! academic.oup.com/genetics/adv...
Thank you to the editors of Genetics for effiecient handling, and to the peer reviewers for excellent feedback
See full paper here! academic.oup.com/genetics/adv...
Thank you to the editors of Genetics for effiecient handling, and to the peer reviewers for excellent feedback
April 3, 2025 at 4:12 PM
So proud of Thomas Nguyen and co-authors on the publication of this manuscript. Now out in Genetics!
See full paper here! academic.oup.com/genetics/adv...
Thank you to the editors of Genetics for effiecient handling, and to the peer reviewers for excellent feedback
See full paper here! academic.oup.com/genetics/adv...
Thank you to the editors of Genetics for effiecient handling, and to the peer reviewers for excellent feedback
Really fun chatting with JP, Enya, and Lawrence!
In this episode, I interviewed Dr. Sheila Teves (@sheilateves.bsky.social) and Dr. Lawrence David (Duke) with a fellow graduate student at Duke, Enya (@erd2.bsky.social)! Words cannot explain how fun it was chatting with other Filipino scientists 😊🇵🇭 #representationmatters
January 12, 2025 at 10:03 PM
Really fun chatting with JP, Enya, and Lawrence!
The Teves lab totally glammed it up at the departmental holiday party!
December 20, 2024 at 12:15 AM
The Teves lab totally glammed it up at the departmental holiday party!
Reposted by Sheila Teves
Finally out! We present EXTRA-seq, a new EXTended Reporter Assay to quantify endogenous enhancer-promoter communication at kb scale!
www.biorxiv.org/content/10.1...
A 🧵about what it can do:
#SynBio #DeepLearning #GeneRegulation
www.biorxiv.org/content/10.1...
A 🧵about what it can do:
#SynBio #DeepLearning #GeneRegulation
EXTRA-seq: a genome-integrated extended massively parallel reporter assay to quantify enhancer-promoter communication
Precise control of gene expression is essential for cellular function, but the mechanisms by which enhancers communicate with promoters to coordinate this process are not fully understood. While seque...
biorxiv.org
December 16, 2024 at 2:39 PM
Finally out! We present EXTRA-seq, a new EXTended Reporter Assay to quantify endogenous enhancer-promoter communication at kb scale!
www.biorxiv.org/content/10.1...
A 🧵about what it can do:
#SynBio #DeepLearning #GeneRegulation
www.biorxiv.org/content/10.1...
A 🧵about what it can do:
#SynBio #DeepLearning #GeneRegulation
Sunshine and rainbows for the little dude
December 16, 2024 at 1:27 AM
Sunshine and rainbows for the little dude
Reposted by Sheila Teves
Ever wondered how transcription choreographs histone modifications? Our work reveals the basis of co-transcriptional H3K36me3 by SETD2. We visualize how a histone writer coordinates with the transcription machinery! This is the magnus opus of @jonmarkert.bsky.social!
tinyurl.com/setd2
tinyurl.com/setd2
December 12, 2024 at 7:16 PM
Ever wondered how transcription choreographs histone modifications? Our work reveals the basis of co-transcriptional H3K36me3 by SETD2. We visualize how a histone writer coordinates with the transcription machinery! This is the magnus opus of @jonmarkert.bsky.social!
tinyurl.com/setd2
tinyurl.com/setd2
Reposted by Sheila Teves
We just posted a preprint about a death mechanism that we find truly surprising.
When you turn off transcription, cells die (duh!)... but did you know this happens due to loss of Pol II itself, not loss of Pol II activity!?!
preprint here and a thread (1/n): www.biorxiv.org/content/10.1...
🧪
When you turn off transcription, cells die (duh!)... but did you know this happens due to loss of Pol II itself, not loss of Pol II activity!?!
preprint here and a thread (1/n): www.biorxiv.org/content/10.1...
🧪
Pol II degradation activates cell death independently from the loss of transcription
Pol II-mediated transcription is essential for eukaryotic life. While loss of transcription is thought to be universally lethal, the associated mechanisms promoting cell death are not yet known. Here,...
www.biorxiv.org
December 13, 2024 at 2:17 PM
We just posted a preprint about a death mechanism that we find truly surprising.
When you turn off transcription, cells die (duh!)... but did you know this happens due to loss of Pol II itself, not loss of Pol II activity!?!
preprint here and a thread (1/n): www.biorxiv.org/content/10.1...
🧪
When you turn off transcription, cells die (duh!)... but did you know this happens due to loss of Pol II itself, not loss of Pol II activity!?!
preprint here and a thread (1/n): www.biorxiv.org/content/10.1...
🧪
Reposted by Sheila Teves
🎄🔔Ho Ho ho!😆 Our final
#FragileNucleosome
seminar of the year! We are excited to host Julia and Nithya, two amazing ECR scientists!
🗓️Don't forget to register: us06web.zoom.us/webinar/regi...
#FragileNucleosome
seminar of the year! We are excited to host Julia and Nithya, two amazing ECR scientists!
🗓️Don't forget to register: us06web.zoom.us/webinar/regi...
December 11, 2024 at 7:41 AM
🎄🔔Ho Ho ho!😆 Our final
#FragileNucleosome
seminar of the year! We are excited to host Julia and Nithya, two amazing ECR scientists!
🗓️Don't forget to register: us06web.zoom.us/webinar/regi...
#FragileNucleosome
seminar of the year! We are excited to host Julia and Nithya, two amazing ECR scientists!
🗓️Don't forget to register: us06web.zoom.us/webinar/regi...
Reposted by Sheila Teves
"Women full professors are 19% more likely than men at the same career stage to leave academia, as compared with 6% for women assistant professors and 10% for associates."
Women faculty feel ‘pushed’ from academia by poor workplace climate
The gender gap in faculty attrition worsens after tenure, according to a new study
www.science.org
December 9, 2024 at 6:27 AM
"Women full professors are 19% more likely than men at the same career stage to leave academia, as compared with 6% for women assistant professors and 10% for associates."
Reposted by Sheila Teves
1/ One more preprint before the holidays! In which we find that chromatin compaction scales with cell size from interphase through mitosis. A collaboration from waaay back (see end of thread) with Melike Lakadamyali @melikel.bsky.social and Jerome Solon. A thread: 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Chromatin Compaction Follows a Power Law Scaling with Cell Size from Interphase Through Mitosis
Coordination of mitotic chromosome compaction with cell size is crucial for proper genome segregation during mitosis. During development, DNA content remains constant but cell size evolves, necessitat...
www.biorxiv.org
December 9, 2024 at 3:20 PM
1/ One more preprint before the holidays! In which we find that chromatin compaction scales with cell size from interphase through mitosis. A collaboration from waaay back (see end of thread) with Melike Lakadamyali @melikel.bsky.social and Jerome Solon. A thread: 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Sheila Teves
Thrilled to share a new study from the lab revealing an H3K18ub-H3K9me3 crosstalk mechanism, roles for UHRF1 and its E3 ligase activity in regulating a hierarchy of repressive histone methylation signaling, and a strategy for targeting SUV39H1/H2 to improve DNMT1 inhibitor efficacy! #epigenetics
DNA hypomethylation promotes UHRF1-and SUV39H1/H2-dependent crosstalk between H3K18ub and H3K9me3 to reinforce heterochromatin states
Liu et al. report that DNMT1 inhibition causes transient accumulation of hemi-methylated
DNA at CpG islands, which stimulates UHRF1-mediated H3K18ub. This enhances SUV39H1/H2
activity, nucleates new H...
www.cell.com
December 4, 2024 at 3:02 AM
Thrilled to share a new study from the lab revealing an H3K18ub-H3K9me3 crosstalk mechanism, roles for UHRF1 and its E3 ligase activity in regulating a hierarchy of repressive histone methylation signaling, and a strategy for targeting SUV39H1/H2 to improve DNMT1 inhibitor efficacy! #epigenetics
Reposted by Sheila Teves
We designed MS2 and PP7 binding coat proteins that are degraded in cells except when bound by their hairpin RNAs.
You can check out our pre-print here: tinyurl.com/27p7hyt9,
and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...
You can check out our pre-print here: tinyurl.com/27p7hyt9,
and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...
November 30, 2024 at 8:48 PM
We designed MS2 and PP7 binding coat proteins that are degraded in cells except when bound by their hairpin RNAs.
You can check out our pre-print here: tinyurl.com/27p7hyt9,
and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...
You can check out our pre-print here: tinyurl.com/27p7hyt9,
and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...
Reposted by Sheila Teves
All editors rely on Pol3 because we use its promoters to transcribe the gRNA from plasmid vectors.
I never thought something so cell-essential would be regulated.
-> food for thought wrt editing cell types in various states.
I never thought something so cell-essential would be regulated.
-> food for thought wrt editing cell types in various states.
🎉Excited to share our new preprint on Biorxiv 🥳
We uncover how RNA Polymerase III (Pol3) dynamically regulates tRNA transcription during heat shock (HS) stress!
So what did we find? 🧵
www.biorxiv.org/content/10.1...
We uncover how RNA Polymerase III (Pol3) dynamically regulates tRNA transcription during heat shock (HS) stress!
So what did we find? 🧵
www.biorxiv.org/content/10.1...
Dynamic regulation of RNA Polymerase III transcription in mouse embryonic stem cells during heat shock stress
Cells respond to many different types of stresses by overhauling gene expression patterns, both at the transcriptional and translational level. Under heat stress, global transcription and translation ...
www.biorxiv.org
November 30, 2024 at 6:37 PM
All editors rely on Pol3 because we use its promoters to transcribe the gRNA from plasmid vectors.
I never thought something so cell-essential would be regulated.
-> food for thought wrt editing cell types in various states.
I never thought something so cell-essential would be regulated.
-> food for thought wrt editing cell types in various states.
🎉Excited to share our new preprint on Biorxiv 🥳
We uncover how RNA Polymerase III (Pol3) dynamically regulates tRNA transcription during heat shock (HS) stress!
So what did we find? 🧵
www.biorxiv.org/content/10.1...
We uncover how RNA Polymerase III (Pol3) dynamically regulates tRNA transcription during heat shock (HS) stress!
So what did we find? 🧵
www.biorxiv.org/content/10.1...
Dynamic regulation of RNA Polymerase III transcription in mouse embryonic stem cells during heat shock stress
Cells respond to many different types of stresses by overhauling gene expression patterns, both at the transcriptional and translational level. Under heat stress, global transcription and translation ...
www.biorxiv.org
November 30, 2024 at 5:21 PM
🎉Excited to share our new preprint on Biorxiv 🥳
We uncover how RNA Polymerase III (Pol3) dynamically regulates tRNA transcription during heat shock (HS) stress!
So what did we find? 🧵
www.biorxiv.org/content/10.1...
We uncover how RNA Polymerase III (Pol3) dynamically regulates tRNA transcription during heat shock (HS) stress!
So what did we find? 🧵
www.biorxiv.org/content/10.1...
Reposted by Sheila Teves
Very interesting paper from @mitchguttman.bsky.social previously in @biorxivpreprint.bsky.social out in Nature Genetics. "ChIP-DIP generates context-specific protein localization maps at consortium scale"
www.nature.com/articles/s41...
www.nature.com/articles/s41...
ChIP-DIP maps binding of hundreds of proteins to DNA simultaneously and identifies diverse gene regulatory elements - Nature Genetics
ChIP-DIP (ChIP done in parallel) is a highly multiplex assay for protein–DNA binding, scalable to hundreds of proteins including modified histones, chromatin regulators and transcription factors, offe...
www.nature.com
November 25, 2024 at 4:59 PM
Very interesting paper from @mitchguttman.bsky.social previously in @biorxivpreprint.bsky.social out in Nature Genetics. "ChIP-DIP generates context-specific protein localization maps at consortium scale"
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Sheila Teves
Machine-guided design of cell-type-targeting cis-regulatory elements
www.nature.com/articles/s41... @natureportfolio.bsky.social
www.nature.com/articles/s41... @natureportfolio.bsky.social
Machine-guided design of cell-type-targeting cis-regulatory elements - Nature
A generalizable framework to prospectively engineer cis-regulatory elements from massively parallel reporter assay models can be used to write fit-for-purpose regulatory code.
www.nature.com
November 26, 2024 at 2:54 PM
Machine-guided design of cell-type-targeting cis-regulatory elements
www.nature.com/articles/s41... @natureportfolio.bsky.social
www.nature.com/articles/s41... @natureportfolio.bsky.social
Beautiful Squamish mountains on the disc golf course
November 25, 2024 at 3:19 AM
Beautiful Squamish mountains on the disc golf course
Reposted by Sheila Teves
The strain on scientific publishing: we set out to characterise the remarkable growth of the scientific literature in the last few years, in spite of declining growth in total scientists. What is going on?
direct.mit.edu/qss/article/...
A 🧵 1/n
#AcademicSky #PhDchat #ScientificPublishing #SciPub
direct.mit.edu/qss/article/...
A 🧵 1/n
#AcademicSky #PhDchat #ScientificPublishing #SciPub
November 19, 2024 at 12:27 PM
The strain on scientific publishing: we set out to characterise the remarkable growth of the scientific literature in the last few years, in spite of declining growth in total scientists. What is going on?
direct.mit.edu/qss/article/...
A 🧵 1/n
#AcademicSky #PhDchat #ScientificPublishing #SciPub
direct.mit.edu/qss/article/...
A 🧵 1/n
#AcademicSky #PhDchat #ScientificPublishing #SciPub
Reposted by Sheila Teves
(1/n) What better way to start posting on Bluesky than to share our recent preprint, in collaboration with @timhughesto.bsky.social, on characterization of the intrinsic DNA binding specificities of 179 human transcription factors using a new assay, GHT-SELEX: www.biorxiv.org/content/10.1...
GHT-SELEX demonstrates unexpectedly high intrinsic sequence specificity and complex DNA binding of many human transcription factors
A long-standing challenge in human regulatory genomics is that transcription factor (TF) DNA-binding motifs are short and degenerate, while the genome is large. Motif scans therefore produce many fals...
www.biorxiv.org
November 21, 2024 at 3:24 AM
(1/n) What better way to start posting on Bluesky than to share our recent preprint, in collaboration with @timhughesto.bsky.social, on characterization of the intrinsic DNA binding specificities of 179 human transcription factors using a new assay, GHT-SELEX: www.biorxiv.org/content/10.1...