Matteo Gentili
@mgentili.bsky.social
🇮🇹 Immunologist in Boston | Senior Scientist at nChroma Bio
All things innate immunity. cGAS/STING and autoinflammation enthusiast.
All things innate immunity. cGAS/STING and autoinflammation enthusiast.
Reposted by Matteo Gentili
Super excited about first Shendure/Baker Lab collaboration & preprint on a multiplex sequencing-based strategy for screening de novo proteome editors in mammalian cells. Kudos to the brilliant Chase Suiter (not here) & @greenahn.bsky.social on the work! Preprint here:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
October 14, 2025 at 6:45 PM
Super excited about first Shendure/Baker Lab collaboration & preprint on a multiplex sequencing-based strategy for screening de novo proteome editors in mammalian cells. Kudos to the brilliant Chase Suiter (not here) & @greenahn.bsky.social on the work! Preprint here:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Matteo Gentili
Virus driven Disruption of Transcription Termination (DoTT) generated cellular Z-RNAs are bona fide ZBP1 ligands driving cell death as a host response to counter viral disruption of the cellular transcriptional machinery @nature.com @foxchasecancer.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
October 13, 2025 at 5:54 PM
Virus driven Disruption of Transcription Termination (DoTT) generated cellular Z-RNAs are bona fide ZBP1 ligands driving cell death as a host response to counter viral disruption of the cellular transcriptional machinery @nature.com @foxchasecancer.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Matteo Gentili
Progression to rheumatoid arthritis in at-risk individuals is defined by systemic inflammation and by T and B cell dysregulation www.science.org/doi/10.1126/...
Progression to rheumatoid arthritis in at-risk individuals is defined by systemic inflammation and by T and B cell dysregulation
Individuals at risk for rheumatoid arthritis exhibit inflammation and immune dysregulation before and during transition to active disease.
www.science.org
September 24, 2025 at 7:46 PM
Progression to rheumatoid arthritis in at-risk individuals is defined by systemic inflammation and by T and B cell dysregulation www.science.org/doi/10.1126/...
Reposted by Matteo Gentili
What an amazing work @iannaconelab.bsky.social &friends just out @natimmunol.nature.com, it was really cool to have a preview of this fantastic work at the 2025 ENII EFIS EJI Summer School! Many congrats Matteo &co! Check it out here 👉 www.nature.com/articles/s41... & here 😉 👇👇👇
July 1, 2025 at 12:08 AM
What an amazing work @iannaconelab.bsky.social &friends just out @natimmunol.nature.com, it was really cool to have a preview of this fantastic work at the 2025 ENII EFIS EJI Summer School! Many congrats Matteo &co! Check it out here 👉 www.nature.com/articles/s41... & here 😉 👇👇👇
Reposted by Matteo Gentili
🧬🖥️ scAtlas of CD34+ cells from 148 people reveals how circulating hematopoietic stem/progenitor cells (cHSPCs) shift with age, sex & disease. Reference model enables myelodysplastic syndrome (MDS) diagnosis. From great Liran Shlush & Amos Tanay @natmed.nature.com: doi.org/10.1038/s415... 🧪
A reference model of circulating hematopoietic stem cells across the lifespan with applications to diagnostics
Nature Medicine - A large single-cell transcriptomic-based reference model for hematopoietic stem and progenitor cells from 148 age- and sex-diverse individuals identifies physiological and...
doi.org
June 30, 2025 at 2:24 PM
🧬🖥️ scAtlas of CD34+ cells from 148 people reveals how circulating hematopoietic stem/progenitor cells (cHSPCs) shift with age, sex & disease. Reference model enables myelodysplastic syndrome (MDS) diagnosis. From great Liran Shlush & Amos Tanay @natmed.nature.com: doi.org/10.1038/s415... 🧪
Reposted by Matteo Gentili
I gave in! After students asking for it, I now made a simple figure design checklist.
To help all scientists w/o graphic skills create clear, accessible, and truthful charts!
-> Out in @nature Cell Biology: rdcu.be/erwl4
#DataVisualization #PhD #SciComm
Thx for review @bethcimini.bsky.social + 2
To help all scientists w/o graphic skills create clear, accessible, and truthful charts!
-> Out in @nature Cell Biology: rdcu.be/erwl4
#DataVisualization #PhD #SciComm
Thx for review @bethcimini.bsky.social + 2
A checklist for designing and improving the visualization of scientific data
Nature Cell Biology - Creating clear and engaging scientific figures is crucial to communicate complex data. In this Comment, I condense principles from design, visual perception and data...
rdcu.be
June 18, 2025 at 8:33 AM
I gave in! After students asking for it, I now made a simple figure design checklist.
To help all scientists w/o graphic skills create clear, accessible, and truthful charts!
-> Out in @nature Cell Biology: rdcu.be/erwl4
#DataVisualization #PhD #SciComm
Thx for review @bethcimini.bsky.social + 2
To help all scientists w/o graphic skills create clear, accessible, and truthful charts!
-> Out in @nature Cell Biology: rdcu.be/erwl4
#DataVisualization #PhD #SciComm
Thx for review @bethcimini.bsky.social + 2
Reposted by Matteo Gentili
Perturb-Multimodal: A platform for pooled genetic screens with imaging and sequencing in intact mammalian tissue @cellcellpress.bsky.social @jswlab.bsky.social @weallen1.bsky.social
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
June 14, 2025 at 4:35 PM
Perturb-Multimodal: A platform for pooled genetic screens with imaging and sequencing in intact mammalian tissue @cellcellpress.bsky.social @jswlab.bsky.social @weallen1.bsky.social
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
Reposted by Matteo Gentili
ENPP1 governs the metabolic regulation of effector T cells in autoimmunity by detecting cytosolic mitochondrial DNA @cp-cellreports.bsky.social
www.cell.com/cell-reports...
www.cell.com/cell-reports...
June 14, 2025 at 4:37 PM
ENPP1 governs the metabolic regulation of effector T cells in autoimmunity by detecting cytosolic mitochondrial DNA @cp-cellreports.bsky.social
www.cell.com/cell-reports...
www.cell.com/cell-reports...
Reposted by Matteo Gentili
If you love #viruses, #ribosomes, and genomic #darkmatter, this thread is for you!! 💫
We're excited to share our new publication developing Massively Parallel Ribosome Profiling (MPRP), which uncovered ~4,000 hidden proteins in ~700 viral genomes. www.science.org/doi/10.1126/...
We're excited to share our new publication developing Massively Parallel Ribosome Profiling (MPRP), which uncovered ~4,000 hidden proteins in ~700 viral genomes. www.science.org/doi/10.1126/...
June 12, 2025 at 9:19 PM
If you love #viruses, #ribosomes, and genomic #darkmatter, this thread is for you!! 💫
We're excited to share our new publication developing Massively Parallel Ribosome Profiling (MPRP), which uncovered ~4,000 hidden proteins in ~700 viral genomes. www.science.org/doi/10.1126/...
We're excited to share our new publication developing Massively Parallel Ribosome Profiling (MPRP), which uncovered ~4,000 hidden proteins in ~700 viral genomes. www.science.org/doi/10.1126/...
Reposted by Matteo Gentili
Out today in @naturegenet.bsky.social -- PERFF-seq! With @tsionabay.bsky.social , @ronanchaligne.bsky.social, Bob Stickels, Meril Takizawa, + Ansu Satpathy, we describe this new assay to study rare populations with programmable nucleic acid cytometry. 1/n
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Transcript-specific enrichment enables profiling of rare cell states via single-cell RNA sequencing - Nature Genetics
Programmable Enrichment via RNA FlowFISH by sequencing (PERFF-seq) isolates rare cells based on RNA marker transcripts for single-cell RNA sequencing profiling of complex tissues, with applicability t...
www.nature.com
January 8, 2025 at 1:30 PM
Out today in @naturegenet.bsky.social -- PERFF-seq! With @tsionabay.bsky.social , @ronanchaligne.bsky.social, Bob Stickels, Meril Takizawa, + Ansu Satpathy, we describe this new assay to study rare populations with programmable nucleic acid cytometry. 1/n
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Matteo Gentili
Thrilled to share our latest @cp-cell.bsky.social.
We present VICAR (VIral-induced Centromeric DNA Amplification and Recognition), a new defense system to detect viruses in the nucleus based on nuclear cGAS.
www.cell.com/cell/abstrac...
We present VICAR (VIral-induced Centromeric DNA Amplification and Recognition), a new defense system to detect viruses in the nucleus based on nuclear cGAS.
www.cell.com/cell/abstrac...
Centromeric DNA amplification triggered by viral proteins activates nuclear cGAS
Herpesvirus proteins disrupt centromeres, triggering centromeric DNA amplification
and local nuclear activation of the nucleic acid sensor cGAS. This reveals an immune
surveillance mechanism in the nu...
www.cell.com
June 2, 2025 at 3:14 PM
Thrilled to share our latest @cp-cell.bsky.social.
We present VICAR (VIral-induced Centromeric DNA Amplification and Recognition), a new defense system to detect viruses in the nucleus based on nuclear cGAS.
www.cell.com/cell/abstrac...
We present VICAR (VIral-induced Centromeric DNA Amplification and Recognition), a new defense system to detect viruses in the nucleus based on nuclear cGAS.
www.cell.com/cell/abstrac...
Reposted by Matteo Gentili
Delighted to share our paper describing how type I interferon promotes caspase-11 hyperactivity in models of PolG-related mitochondrial disease. Thanks to all co-authors and the POLG Foundation for for their support. www.nature.com/articles/s41...
Caspase-11 drives macrophage hyperinflammation in models of Polg-related mitochondrial disease - Nature Communications
Mitochondrial diseases lead to chronic health impairment, aggravated by infections and other environmental exposures. Here authors show, in a mouse model of polymerase gamma (Polg)-related mitochondri...
www.nature.com
June 1, 2025 at 1:16 AM
Delighted to share our paper describing how type I interferon promotes caspase-11 hyperactivity in models of PolG-related mitochondrial disease. Thanks to all co-authors and the POLG Foundation for for their support. www.nature.com/articles/s41...
Reposted by Matteo Gentili
#WeekendRead! #InterferonPower! Stunnenberg Pickkers Kox, Keramati &co show @natimmunol.bsky.org that 2 LPS injections in volunteers mimic the proinflammatory & immunosuppressive phases of #sepsis & found that IFNb treatment restores immune responsiveness in the suppressive phase!
Systemic inflammation impairs myelopoiesis and interferon type I responses in humans - Nature Immunology
Stunnenberg et al. use a model of lipopolysaccharide injection in humans to characterize the transcriptomic landscape of bone marrow and blood immune cells during the hyperinflammatory and immunosuppr...
www.nature.com
May 10, 2025 at 2:25 PM
#WeekendRead! #InterferonPower! Stunnenberg Pickkers Kox, Keramati &co show @natimmunol.bsky.org that 2 LPS injections in volunteers mimic the proinflammatory & immunosuppressive phases of #sepsis & found that IFNb treatment restores immune responsiveness in the suppressive phase!
Reposted by Matteo Gentili
Gomez-Diaz, @v-hornung.bsky.social et al. show that RNase T2 restricts TLR13-mediated #autoinflammation in vivo. rupress.org/jem/article/...
📘 In JEM #Immunology Collection: rupress.org/jem/collecti...
#AAI2025
📘 In JEM #Immunology Collection: rupress.org/jem/collecti...
#AAI2025
May 6, 2025 at 10:15 PM
Gomez-Diaz, @v-hornung.bsky.social et al. show that RNase T2 restricts TLR13-mediated #autoinflammation in vivo. rupress.org/jem/article/...
📘 In JEM #Immunology Collection: rupress.org/jem/collecti...
#AAI2025
📘 In JEM #Immunology Collection: rupress.org/jem/collecti...
#AAI2025
Reposted by Matteo Gentili
"Immune signaling through nucleotide-based second messengers is a widespread defense mechanism observed across organisms, from bacteria to animals. Zeng et al. discovered a base-modified nucleotide, deoxyinosine 5′-triphosphate (dITP), that acts as an antiviral immune signal in bacteria"
#Immunology
#Immunology
Base-modified nucleotides mediate immune signaling in bacteria
Signaling from pathogen sensing to effector activation is a fundamental principle of cellular immunity. Whereas cyclic (oligo)nucleotides have emerged as key signaling molecules, the existence of othe...
www.science.org
April 26, 2025 at 9:43 AM
"Immune signaling through nucleotide-based second messengers is a widespread defense mechanism observed across organisms, from bacteria to animals. Zeng et al. discovered a base-modified nucleotide, deoxyinosine 5′-triphosphate (dITP), that acts as an antiviral immune signal in bacteria"
#Immunology
#Immunology
Reposted by Matteo Gentili
#weekendreading📑🔬
Base-modified nucleotides mediate immune signaling in bacteria
@science.org #microsky
www.science.org/doi/10.1126/...
Base-modified nucleotides mediate immune signaling in bacteria
@science.org #microsky
www.science.org/doi/10.1126/...
Base-modified nucleotides mediate immune signaling in bacteria
Signaling from pathogen sensing to effector activation is a fundamental principle of cellular immunity. Whereas cyclic (oligo)nucleotides have emerged as key signaling molecules, the existence of othe...
www.science.org
April 25, 2025 at 8:35 AM
#weekendreading📑🔬
Base-modified nucleotides mediate immune signaling in bacteria
@science.org #microsky
www.science.org/doi/10.1126/...
Base-modified nucleotides mediate immune signaling in bacteria
@science.org #microsky
www.science.org/doi/10.1126/...
Reposted by Matteo Gentili
ZBP1 senses splicing aberration through Z-RNA to promote cell death
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
April 24, 2025 at 12:21 AM
ZBP1 senses splicing aberration through Z-RNA to promote cell death
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
Reposted by Matteo Gentili
AAV vectors trigger DNA damage response-dependent pro-inflammatory signaling in human iPSC-derived CNS models and mouse brain @natcomms.nature.com @kajastelab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
April 18, 2025 at 4:32 PM
AAV vectors trigger DNA damage response-dependent pro-inflammatory signaling in human iPSC-derived CNS models and mouse brain @natcomms.nature.com @kajastelab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Matteo Gentili
Now online! Integrator loss leads to dsRNA formation that triggers the integrated stress response
Integrator loss leads to dsRNA formation that triggers the integrated stress response
Integrator (INT) prevents the escape of immature RNAPII to ensure full-length mRNA production. INT deficiency causes defects in RNAPII maturation that lead to premature termination, accumulation of immunogenic dsRNA, and activation of the integrated stress response (ISR) observed in patient cells.
dlvr.it
April 14, 2025 at 10:38 PM
Now online! Integrator loss leads to dsRNA formation that triggers the integrated stress response
Reposted by Matteo Gentili
Setdb2 regulates trained immunity by 2 complementary mechanisms:
1: increase glycolytic & inflammatory pathway genes via enhancer-promoter looping
2: increased promoter associated H3K9 methylation & repressed interferon response papers.ssrn.com/sol3/papers....
1: increase glycolytic & inflammatory pathway genes via enhancer-promoter looping
2: increased promoter associated H3K9 methylation & repressed interferon response papers.ssrn.com/sol3/papers....
April 8, 2025 at 12:02 AM
Setdb2 regulates trained immunity by 2 complementary mechanisms:
1: increase glycolytic & inflammatory pathway genes via enhancer-promoter looping
2: increased promoter associated H3K9 methylation & repressed interferon response papers.ssrn.com/sol3/papers....
1: increase glycolytic & inflammatory pathway genes via enhancer-promoter looping
2: increased promoter associated H3K9 methylation & repressed interferon response papers.ssrn.com/sol3/papers....
Reposted by Matteo Gentili
#WeekendRead! #NotOnlyInterferons! @nanyanlab.bsky.social &co show @cp-molecularcell.bsky.social that other than inducing pathogenic IFNs in lysosomal storage disorders, STING also play protective functions as a proton channel activating TFEB & inducing lysosomal repair www.cell.com/molecular-ce...
STING mediates lysosomal quality control and recovery through its proton channel function and TFEB activation in lysosomal storage disorders
STING signaling in the innate immune system is well known for its role in the antiviral
type I interferon response. Tang et al. find that STING acts as an immune sensor of
lysosomal activity. When the...
www.cell.com
April 5, 2025 at 3:08 PM
#WeekendRead! #NotOnlyInterferons! @nanyanlab.bsky.social &co show @cp-molecularcell.bsky.social that other than inducing pathogenic IFNs in lysosomal storage disorders, STING also play protective functions as a proton channel activating TFEB & inducing lysosomal repair www.cell.com/molecular-ce...
Reposted by Matteo Gentili
An atlas of single-cell eQTLs dissects autoimmune disease genes and identifies novel drug classes for treatment
www.cell.com/cell-genomic...
www.cell.com/cell-genomic...
March 28, 2025 at 1:55 AM
An atlas of single-cell eQTLs dissects autoimmune disease genes and identifies novel drug classes for treatment
www.cell.com/cell-genomic...
www.cell.com/cell-genomic...
Reposted by Matteo Gentili
THREAD
The numbers are in. @bsky.app research sharing volumes vs X Formerly Twitter
In March 2024, on most days, Bluesky hosts more posts linked to research published in 2025 than X.
By quite a lot.
Release the Kraken...
#AcademicSky #HigherEd #Altmetrics
1/11
The numbers are in. @bsky.app research sharing volumes vs X Formerly Twitter
In March 2024, on most days, Bluesky hosts more posts linked to research published in 2025 than X.
By quite a lot.
Release the Kraken...
#AcademicSky #HigherEd #Altmetrics
1/11
March 27, 2025 at 5:22 PM
THREAD
The numbers are in. @bsky.app research sharing volumes vs X Formerly Twitter
In March 2024, on most days, Bluesky hosts more posts linked to research published in 2025 than X.
By quite a lot.
Release the Kraken...
#AcademicSky #HigherEd #Altmetrics
1/11
The numbers are in. @bsky.app research sharing volumes vs X Formerly Twitter
In March 2024, on most days, Bluesky hosts more posts linked to research published in 2025 than X.
By quite a lot.
Release the Kraken...
#AcademicSky #HigherEd #Altmetrics
1/11
Reposted by Matteo Gentili
In new study led by Brendan Larsen in collab w Veesler & Crowe labs, we map functional & antigenic landscape of Nipah virus receptor binding protein (RBP)
www.biorxiv.org/content/10.1...
Results elucidate constraints on RBP function & provide insight into protein’s evolutionary potential
www.biorxiv.org/content/10.1...
Results elucidate constraints on RBP function & provide insight into protein’s evolutionary potential
Functional and antigenic landscape of the Nipah virus receptor binding protein
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
www.biorxiv.org
April 20, 2024 at 1:49 AM
In new study led by Brendan Larsen in collab w Veesler & Crowe labs, we map functional & antigenic landscape of Nipah virus receptor binding protein (RBP)
www.biorxiv.org/content/10.1...
Results elucidate constraints on RBP function & provide insight into protein’s evolutionary potential
www.biorxiv.org/content/10.1...
Results elucidate constraints on RBP function & provide insight into protein’s evolutionary potential
Reposted by Matteo Gentili
Wonder which #innate responses in your animal models are shared with or distinct from humans? We did too! To answer, we teamed up with @mcampomd.bsky.social and the Hawn lab to compare human and murine alveolar and monocyte-derived #macrophage responses to #Mtb. (1/4)
#TBSky #ImmunoSky #NewPI x 2
#TBSky #ImmunoSky #NewPI x 2
Shared and distinct responses of human and murine alveolar macrophages and monocyte-derived macrophages to Mycobacterium tuberculosis https://www.biorxiv.org/content/10.1101/2025.02.28.640814v1
March 11, 2025 at 9:38 PM
Wonder which #innate responses in your animal models are shared with or distinct from humans? We did too! To answer, we teamed up with @mcampomd.bsky.social and the Hawn lab to compare human and murine alveolar and monocyte-derived #macrophage responses to #Mtb. (1/4)
#TBSky #ImmunoSky #NewPI x 2
#TBSky #ImmunoSky #NewPI x 2