Kukar Lab
@kukarlab.bsky.social
Working to understand and cure Alzheimer's disease, Frontotemporal dementia, ALS. #Progranulin #FUS #TDP43 #lysosomes #RNA #iPSCs #inflammation
Reposted by Kukar Lab
Wonderful to see our paper on the #organelle signatures of #neurons and #astrocytes out in final form - congratulations, Shannon Rhoads and team!🎉 t.co/BPxKlbU6Ou
September 17, 2025 at 5:38 PM
Wonderful to see our paper on the #organelle signatures of #neurons and #astrocytes out in final form - congratulations, Shannon Rhoads and team!🎉 t.co/BPxKlbU6Ou
Reposted by Kukar Lab
ABCA7 variants impact phosphatidylcholine and mitochondria in neurons www.nature.com/articles/s41...
ABCA7 variants impact phosphatidylcholine and mitochondria in neurons - Nature
Loss-of-function variants of ABCA7, associated with Alzheimer’s disease, result in disrupted lipid metabolism, mitochondrial function, DNA repair and synaptic signalling pathways in the human brain.
www.nature.com
September 15, 2025 at 8:06 PM
ABCA7 variants impact phosphatidylcholine and mitochondria in neurons www.nature.com/articles/s41...
Reposted by Kukar Lab
Akayama, Shima, Nakamura et al. show that TFEB is regulated by at least two different regulatory mechanisms during lysosomal damage: ATG conjugation system–dependent/independent regulation. rupress.org/jcb/article/...
#Lysosomes #Autophagy
#Lysosomes #Autophagy
August 29, 2025 at 5:01 PM
Akayama, Shima, Nakamura et al. show that TFEB is regulated by at least two different regulatory mechanisms during lysosomal damage: ATG conjugation system–dependent/independent regulation. rupress.org/jcb/article/...
#Lysosomes #Autophagy
#Lysosomes #Autophagy
Reposted by Kukar Lab
NEW: More than 750 current and former staff of HHS, including @CDCgov, and NIH, have signed a letter to RFKJr and members of Congress accusing him of endangering the lives of employees and the American people following the Aug 8 attack on the CDC.
www.savehhs.org/letter/hhs-s...
www.savehhs.org/letter/hhs-s...
HHS Staff Response to the August 8th Attack — Save HHS
Public servants and allies are standing together to demand work environments free from violence, accountability for leaders and policymakers who put everyday Americans in danger, and public health lea...
www.savehhs.org
August 20, 2025 at 1:00 PM
NEW: More than 750 current and former staff of HHS, including @CDCgov, and NIH, have signed a letter to RFKJr and members of Congress accusing him of endangering the lives of employees and the American people following the Aug 8 attack on the CDC.
www.savehhs.org/letter/hhs-s...
www.savehhs.org/letter/hhs-s...
Reposted by Kukar Lab
NEW: The CDC shooting was much worse than initially reported. 181 rounds. 150 windows hit. Staff are furious at RFK Jr. and Trump becuz their long-running fears about anti-vax rhetoric turning deadly seem to be vindicated. @laurenweberhp.bluesky.social + Me.
www.washingtonpost.com/health/2025/...
www.washingtonpost.com/health/2025/...
After CDC shooting, its employees turn their anger to RFK Jr. and Trump
Investigators allege the shooter targeted the agency because of his coronavirus vaccine beliefs, according to two CDC officials briefed on the case.
www.washingtonpost.com
August 12, 2025 at 11:41 AM
NEW: The CDC shooting was much worse than initially reported. 181 rounds. 150 windows hit. Staff are furious at RFK Jr. and Trump becuz their long-running fears about anti-vax rhetoric turning deadly seem to be vindicated. @laurenweberhp.bluesky.social + Me.
www.washingtonpost.com/health/2025/...
www.washingtonpost.com/health/2025/...
Thank you all for the concern. We are all alive and OK-ish following the targeted attack on the CDC in Atlanta.
August 9, 2025 at 1:27 PM
Thank you all for the concern. We are all alive and OK-ish following the targeted attack on the CDC in Atlanta.
Reposted by Kukar Lab
The National Institutes of Health’s budget, and structure, would be preserved under a Senate bill for the 2026 fiscal year.
With boost to NIH budget, Senate panel rejects Trump’s plan to slash agency
Republicans on spending committee push back against proposed reorganization and overhead cuts
scim.ag
July 31, 2025 at 9:37 PM
The National Institutes of Health’s budget, and structure, would be preserved under a Senate bill for the 2026 fiscal year.
Reposted by Kukar Lab
New TYP lab publication led by PhD student @alexlish.bsky.social out today in @cp-cellreports.bsky.social! We present a reproducible neuron-astrocyte-microglia tri-culture model to better reproduce intercellular interactions in Alzheimer's disease. Read more here: www.cell.com/cell-reports...
Astrocyte induction of disease-associated microglia is suppressed by acute exposure to fAD neurons in human iPSC triple cultures
Lish et al. present a co-culture system of iPSC-derived human microglia, astrocytes,
and neurons to investigate intercellular communication across different environmental
and genetic contexts. Transcr...
www.cell.com
June 5, 2025 at 12:49 AM
New TYP lab publication led by PhD student @alexlish.bsky.social out today in @cp-cellreports.bsky.social! We present a reproducible neuron-astrocyte-microglia tri-culture model to better reproduce intercellular interactions in Alzheimer's disease. Read more here: www.cell.com/cell-reports...
Reposted by Kukar Lab
Thrilled this paper is out! We solved the first cocrystal structure of FSP1 with an inhibitor (FSEN1), providing mechanistic insight & a foundation for medchem. Led by Amalia Megarioti & Sitao Zhang. A terrific collaboration with Da Jia.
Brief Skytorial!
1/9
Brief Skytorial!
1/9
Cocrystal structure reveals the mechanism of FSP1 inhibition by FSEN1 | PNAS
FSP1 is an FAD-dependent oxidoreductase that uses NAD(P)H to regenerate the reduced
forms of lipophilic quinone antioxidants, such as coenzyme Q10 ...
www.pnas.org
May 29, 2025 at 6:02 PM
Thrilled this paper is out! We solved the first cocrystal structure of FSP1 with an inhibitor (FSEN1), providing mechanistic insight & a foundation for medchem. Led by Amalia Megarioti & Sitao Zhang. A terrific collaboration with Da Jia.
Brief Skytorial!
1/9
Brief Skytorial!
1/9
Reposted by Kukar Lab
New TYP lab paper out today in @cp-neuron.bsky.social led by PhD student @alexlish.bsky.social! We took a deep dive into the mechanisms underlying CLU in AD using innovative iPSC, mouse, and human data approaches. Be sure to give it a read!
www.cell.com/neuron/abstr...
www.cell.com/neuron/abstr...
CLU alleviates Alzheimer’s disease-relevant processes by modulating astrocyte reactivity and microglia-dependent synaptic density
Lish et al. reveal that AD-protective CLU alleles enhance CLU upregulation in response
to accumulated neuropathology, thereby dampening inflammatory signaling between microglia
and astrocytes. Using s...
www.cell.com
April 30, 2025 at 3:56 PM
New TYP lab paper out today in @cp-neuron.bsky.social led by PhD student @alexlish.bsky.social! We took a deep dive into the mechanisms underlying CLU in AD using innovative iPSC, mouse, and human data approaches. Be sure to give it a read!
www.cell.com/neuron/abstr...
www.cell.com/neuron/abstr...
Reposted by Kukar Lab
Reposted by Kukar Lab
We've been studying mechanisms of resilience that protect lysosomes from damage and wrote a review on the topic. We consider lysosomal stress and damage on a spectrum. Please help me share as this is my first post 😀 and I don't have many followers! 🙏 Open Access: www.embopress.org/doi/full/10....
February 27, 2025 at 3:54 PM
We've been studying mechanisms of resilience that protect lysosomes from damage and wrote a review on the topic. We consider lysosomal stress and damage on a spectrum. Please help me share as this is my first post 😀 and I don't have many followers! 🙏 Open Access: www.embopress.org/doi/full/10....
Reposted by Kukar Lab
Stand Up For Science next Friday (3/7)! In 2024, Georgia institutions received just over $1 billion in funding and had more than $30 billion in economic impact in GA. That's a PHENOMENAL return for tax payers- let's share that news!
February 27, 2025 at 1:36 AM
Stand Up For Science next Friday (3/7)! In 2024, Georgia institutions received just over $1 billion in funding and had more than $30 billion in economic impact in GA. That's a PHENOMENAL return for tax payers- let's share that news!
Reposted by Kukar Lab
Harvard Health reports on the recent ruling that the makers of Prevagen can no longer claim their #supplement improves #memory. The author recommends being wary about supplement marketing claims, which are often false, misleading, or based on insufficient research.
February 20, 2025 at 9:00 PM
Harvard Health reports on the recent ruling that the makers of Prevagen can no longer claim their #supplement improves #memory. The author recommends being wary about supplement marketing claims, which are often false, misleading, or based on insufficient research.
Reposted by Kukar Lab
Make sure you, your trainees, and anyone else who might listen to you understands what "indirects" actually are, how they are established, and what Universities contribute. H/T @rodallab.bsky.social
Please share widely!
www.youtube.com/watch?v=yxTD...
Please share widely!
www.youtube.com/watch?v=yxTD...
Facilities and Administrative (F&A) Costs Explainer
YouTube video by Association of American Universities
www.youtube.com
February 11, 2025 at 3:00 PM
Make sure you, your trainees, and anyone else who might listen to you understands what "indirects" actually are, how they are established, and what Universities contribute. H/T @rodallab.bsky.social
Please share widely!
www.youtube.com/watch?v=yxTD...
Please share widely!
www.youtube.com/watch?v=yxTD...
Reposted by Kukar Lab
Donald Trump has been sworn in, and his new administration has immediately turned its sights on the NIH. The danger to US biomedical research has never been more acute. sciencebasedmedicine.org/the-attack-o...
The attack on the NIH has begun | Science-Based Medicine
Donald Trump has been sworn in, and his new administration turned its gaze on the NIH. The danger to US biomedical research has never been more acute.
sciencebasedmedicine.org
January 27, 2025 at 4:17 PM
Donald Trump has been sworn in, and his new administration has immediately turned its sights on the NIH. The danger to US biomedical research has never been more acute. sciencebasedmedicine.org/the-attack-o...
Reposted by Kukar Lab
Started off this morning emailing my Senators and Congressman about the NIH and the potential damage being done by unnecessary disruptions.
They need to hear from us loud and clear. Constituents matter to them as one of the primary purposes of a politician is to get re-elected (and its their job).
They need to hear from us loud and clear. Constituents matter to them as one of the primary purposes of a politician is to get re-elected (and its their job).
January 23, 2025 at 11:26 AM
Started off this morning emailing my Senators and Congressman about the NIH and the potential damage being done by unnecessary disruptions.
They need to hear from us loud and clear. Constituents matter to them as one of the primary purposes of a politician is to get re-elected (and its their job).
They need to hear from us loud and clear. Constituents matter to them as one of the primary purposes of a politician is to get re-elected (and its their job).
Excited to share the lab's recent publication. Granulins are critical for lysosome function and brain health to prevent neurodegeneration. Our data suggest that granulin peptides could be used to treat frontotemporal dementia (FTD), Parkinson’s disease, Alzheimer’s disease.
t.co/pN28kabjIA
t.co/pN28kabjIA
November 21, 2024 at 8:48 PM
Excited to share the lab's recent publication. Granulins are critical for lysosome function and brain health to prevent neurodegeneration. Our data suggest that granulin peptides could be used to treat frontotemporal dementia (FTD), Parkinson’s disease, Alzheimer’s disease.
t.co/pN28kabjIA
t.co/pN28kabjIA
Reposted by Kukar Lab
Great work from Mark Sands’ lab showing a 50% reduction in lysosomal enzymes puts you at risk for Alzheimer’s and other neurodegenerative diseases.
Haploinsufficiency of lysosomal enzyme genes in Alzheimers disease https://www.biorxiv.org/content/10.1101/2024.11.16.623962v1
Haploinsufficiency of lysosomal enzyme genes in Alzheimers disease https://www.biorxiv.org/content/10.1101/2024.11.16.623962v1
There is growing evidence suggesting that the lysosome or lysosome dysfunction is associated with Al
www.biorxiv.org
November 18, 2024 at 11:34 PM
Great work from Mark Sands’ lab showing a 50% reduction in lysosomal enzymes puts you at risk for Alzheimer’s and other neurodegenerative diseases.
Reposted by Kukar Lab
Very happy to share our paper on Familial British Dementia (FBD) which is online today!
Using patient iPSC,we show the amyloidogenic peptide in this disease -ABri -is mainly produced by microglia…
Never heard of FBD? You won’t be the only one… explainer incoming!
link.springer.com/article/10.1...
Using patient iPSC,we show the amyloidogenic peptide in this disease -ABri -is mainly produced by microglia…
Never heard of FBD? You won’t be the only one… explainer incoming!
link.springer.com/article/10.1...
Microglia contribute to the production of the amyloidogenic ABri peptide in familial British dementia - Acta Neuropathologica
Mutations in ITM2B cause familial British, Danish, Chinese, and Korean dementias. In familial British dementia (FBD), a mutation in the stop codon of the ITM2B gene (also known as BRI2) causes a C-ter...
link.springer.com
November 15, 2024 at 12:53 PM
Very happy to share our paper on Familial British Dementia (FBD) which is online today!
Using patient iPSC,we show the amyloidogenic peptide in this disease -ABri -is mainly produced by microglia…
Never heard of FBD? You won’t be the only one… explainer incoming!
link.springer.com/article/10.1...
Using patient iPSC,we show the amyloidogenic peptide in this disease -ABri -is mainly produced by microglia…
Never heard of FBD? You won’t be the only one… explainer incoming!
link.springer.com/article/10.1...
Reposted by Kukar Lab
My latest paper, deep proteomic analysis of mouse and human microglia: www.cell.com/cell-reports...
Deep proteomic analysis of microglia reveals fundamental biological differences between model systems
Lloyd et al. generated a comprehensive microglia proteome database, identifying over
11,000 proteins. Fundamental differences between ex vivo and in vitro microglia associated
with protein synthesis, ...
www.cell.com
November 13, 2024 at 9:24 AM
My latest paper, deep proteomic analysis of mouse and human microglia: www.cell.com/cell-reports...