High sugar–containing foods are readily consumed, even after meals and beyond fullness sensation (e.g., as desserts). Although reward-driven processing of palatable foods can promote overeating, the n...

Data-independent acquisition (DIA) on ion mobility mass spectrometers enables deep proteome coverage and high data completeness in large-scale proteomics studies. For advanced acquisition schemes such as parallel accumulation serial fragmentation-based DIA (diaPASEF) stability of ion mobility (1/K0) over time is crucial for consistent data quality. We found that minor changes in environmental air pressure systematically affect the vacuum pressure in the TIMS analyzer, causing ion mobility shifts. By comparing experimental ion mobilities with historical weather data, we attributed observed drifts to fluctuations in the ground air pressure. Moderate air pressure changes of e.g. fifteen mbar induce ion mobility shifts of 0.025 Vs/cm2. These drifts negatively impact peptide quantification across consecutively acquired samples due to drift-dependent abundance changes and increased missing values for ions located at the boundaries of diaPASEF isolation windows, which cannot be corrected by postprocessing. To address this, we applied an in-batch mobility autocalibration feature on a run-wise basis, leading to full elimination of ion mobility drifts.

En handel mellem USA og Danmark i 1900-tallet hjalp Danmark til at sikre sig Grønland, som i mange år havde været en dansk koloni.

Here, I fixed it for ya! Even though I can clearly see why I also wouldn't have recommended Ben Orsburn as a reviewer for this one, I do a...

The oxidation status of N-terminal cysteines directly dictates protein stability via arginylation and proteasomal degradation. However, only a handful of proteins have been shown to be regulated via t...

France has already banned it — now industry is lobbying hard to ensure the EU doesn’t do the same.

Do you have a passion for drug discovery and want to contribute to diverse research projects embedded in the Oncode-Accelerator consortium using mass-spectrometry-based chemoproteomics? Do you want to work in an international team of excellent, interdisciplinary researchers? You might be our new Postdoctoral…

The furor over a Nobel Prize winner’s derailed career lets scientists off the hook for their own responsibilities to fix a broken academic reward system