Ishier Raote
@ishier.bsky.social
Institut Jacques Monod, Paris
@ijmonod.bsky.social
https://www.ijm.fr/raote-lab/
Collagen secretion, membrane trafficking
Formerly @crg.eu
@ijmonod.bsky.social
https://www.ijm.fr/raote-lab/
Collagen secretion, membrane trafficking
Formerly @crg.eu
Reposted by Ishier Raote
The growth of Paris from 250 AD to the present day in 3 minutes.
🔎 CITY 3D TIMELAPSE
🔎 CITY 3D TIMELAPSE
January 17, 2026 at 8:15 AM
The growth of Paris from 250 AD to the present day in 3 minutes.
🔎 CITY 3D TIMELAPSE
🔎 CITY 3D TIMELAPSE
Reposted by Ishier Raote
We made nanobodies that make your heart🫀 stop 🛑beating. Keep reading to learn how this taught us about how large membrane proteins like ion channels are folded and assembled in human cells. Beyond excited to share my lab's first preprint: Please 🔄 bit.ly/49A0PtD
January 16, 2026 at 10:57 PM
We made nanobodies that make your heart🫀 stop 🛑beating. Keep reading to learn how this taught us about how large membrane proteins like ion channels are folded and assembled in human cells. Beyond excited to share my lab's first preprint: Please 🔄 bit.ly/49A0PtD
Reposted by Ishier Raote
0/10
🥳 our #exocyst paper out in @cp-cell.bsky.social, led by Marta, Sebas & @sasmeek.bsky.social in collab with @jonasries.bsky.social, @cmanzo.bsky.social & Castaño-Díez labs #Quantitative_Cell_Biology
Continuum architecture dynamics of vesicle tethering in exocytosis
www.cell.com/cell/fulltex...
🥳 our #exocyst paper out in @cp-cell.bsky.social, led by Marta, Sebas & @sasmeek.bsky.social in collab with @jonasries.bsky.social, @cmanzo.bsky.social & Castaño-Díez labs #Quantitative_Cell_Biology
Continuum architecture dynamics of vesicle tethering in exocytosis
www.cell.com/cell/fulltex...
Continuum architecture dynamics of vesicle tethering in exocytosis
Data from complementary imaging techniques were integrated into a model that resolves
the dynamic exocyst ensemble and membrane architecture during exocytosis, an essential
cellular pathway. Sec18 med...
www.cell.com
January 16, 2026 at 5:28 PM
0/10
🥳 our #exocyst paper out in @cp-cell.bsky.social, led by Marta, Sebas & @sasmeek.bsky.social in collab with @jonasries.bsky.social, @cmanzo.bsky.social & Castaño-Díez labs #Quantitative_Cell_Biology
Continuum architecture dynamics of vesicle tethering in exocytosis
www.cell.com/cell/fulltex...
🥳 our #exocyst paper out in @cp-cell.bsky.social, led by Marta, Sebas & @sasmeek.bsky.social in collab with @jonasries.bsky.social, @cmanzo.bsky.social & Castaño-Díez labs #Quantitative_Cell_Biology
Continuum architecture dynamics of vesicle tethering in exocytosis
www.cell.com/cell/fulltex...
Asahi Super Dry: helping people through Dry January since 1987.
January 15, 2026 at 8:46 PM
Asahi Super Dry: helping people through Dry January since 1987.
Exciting preprint from @felixmendu.bsky.social and Yuichi Wakana! A TGN carrier whose budding is driven by ER–Golgi lipid transfer, PKD activity, and a caveolin-based oligomeric coat!
A PKD-caveolin axis drives secretory carrier biogenesis at the TGN
www.biorxiv.org/content/10.6...
A PKD-caveolin axis drives secretory carrier biogenesis at the TGN
www.biorxiv.org/content/10.6...
January 14, 2026 at 4:20 PM
Exciting preprint from @felixmendu.bsky.social and Yuichi Wakana! A TGN carrier whose budding is driven by ER–Golgi lipid transfer, PKD activity, and a caveolin-based oligomeric coat!
A PKD-caveolin axis drives secretory carrier biogenesis at the TGN
www.biorxiv.org/content/10.6...
A PKD-caveolin axis drives secretory carrier biogenesis at the TGN
www.biorxiv.org/content/10.6...
Reposted by Ishier Raote
Have you ever heard that women have more chances than men of getting grants or positions in academia? Of course you have, and here are my personal views on this. With the shocking revelation that no, it is NOT TRUE ! (1/n)
January 13, 2026 at 6:36 PM
Have you ever heard that women have more chances than men of getting grants or positions in academia? Of course you have, and here are my personal views on this. With the shocking revelation that no, it is NOT TRUE ! (1/n)
Fun preprint from @marius-lemberg.bsky.social! TMED9 drives Golgi-bypass secretion of proTGFα via a RHBDL4-independent pathway requiring GRASP65, autophagy machinery and ALIX.
A Golgi-bypass secretion mechanism for proTGFα involving TMED9 and GRASP65
www.biorxiv.org/content/10.6...
A Golgi-bypass secretion mechanism for proTGFα involving TMED9 and GRASP65
www.biorxiv.org/content/10.6...
January 13, 2026 at 8:08 PM
Fun preprint from @marius-lemberg.bsky.social! TMED9 drives Golgi-bypass secretion of proTGFα via a RHBDL4-independent pathway requiring GRASP65, autophagy machinery and ALIX.
A Golgi-bypass secretion mechanism for proTGFα involving TMED9 and GRASP65
www.biorxiv.org/content/10.6...
A Golgi-bypass secretion mechanism for proTGFα involving TMED9 and GRASP65
www.biorxiv.org/content/10.6...
Exciting preprint from @franbottanelli.bsky.social and @annespang.bsky.social! ARF-1 regulates FERARI-mediated endosomal kiss-and-run recycling by controlling selective cargo loading into vesicles.
ARF-1 Coordinates Cargo Sorting at FERARI Endosomal Recycling Hubs
www.biorxiv.org/content/10.6...
ARF-1 Coordinates Cargo Sorting at FERARI Endosomal Recycling Hubs
www.biorxiv.org/content/10.6...
January 13, 2026 at 7:46 PM
Exciting preprint from @franbottanelli.bsky.social and @annespang.bsky.social! ARF-1 regulates FERARI-mediated endosomal kiss-and-run recycling by controlling selective cargo loading into vesicles.
ARF-1 Coordinates Cargo Sorting at FERARI Endosomal Recycling Hubs
www.biorxiv.org/content/10.6...
ARF-1 Coordinates Cargo Sorting at FERARI Endosomal Recycling Hubs
www.biorxiv.org/content/10.6...
Reposted by Ishier Raote
I am very happy (and a bit scared) to present to you what we have been working on over the last 4 years. This manuscript is exactly what I dreamt of when I started the lab and I could not be happier and prouder of the outcome!
Evolutionary dynamics of temporal transcription factor series in the insect optic lobe https://www.biorxiv.org/content/10.64898/2026.01.08.698497v1
January 10, 2026 at 9:43 AM
I am very happy (and a bit scared) to present to you what we have been working on over the last 4 years. This manuscript is exactly what I dreamt of when I started the lab and I could not be happier and prouder of the outcome!
Reposted by Ishier Raote
Early bird deadline in 10 days!! This is going to be amazing!
Early-bird deadline to join @jcellsci.bsky.social at their meeting on Imaging Cell Dynamics in Spain in May is Friday 16 January. Apply to attend at:
biologists.com/meetings/jcs...
#JCSimaging
biologists.com/meetings/jcs...
#JCSimaging
January 6, 2026 at 7:12 PM
Early bird deadline in 10 days!! This is going to be amazing!
Reposted by Ishier Raote
Excited to start 2026 out with a new manuscript from our lab, please check out this thread from superstar postdoc @rvig.bsky.social about how he discovered the function of a conserved secretory protein, and how this also led him to the identification of a new family of GAP proteins!
Mind the GAP 🚧🕳️🚧
Excited to share the latest preprint from the Fromme Lab (@fromme-lab.bsky.social), showing that the DENN domain protein Avl9 functions as an Arf-GAP, revising long-standing assumptions about DENN protein function, 🧵:
www.biorxiv.org/content/10.6...
Excited to share the latest preprint from the Fromme Lab (@fromme-lab.bsky.social), showing that the DENN domain protein Avl9 functions as an Arf-GAP, revising long-standing assumptions about DENN protein function, 🧵:
www.biorxiv.org/content/10.6...
Avl9 defines a family of GTPase-activating proteins that regulate diverse cell biological functions
Ras-related GTPases are molecular switches regulating hundreds of signaling and trafficking pathways in cells. Many GTPase regulators remain to be identified despite extensive genetic and biochemical ...
www.biorxiv.org
January 6, 2026 at 3:54 PM
Excited to start 2026 out with a new manuscript from our lab, please check out this thread from superstar postdoc @rvig.bsky.social about how he discovered the function of a conserved secretory protein, and how this also led him to the identification of a new family of GAP proteins!
Reposted by Ishier Raote
📢 We're hiring a postdoc (33 months) in human evolutionary genomics / paleogenomics / statistical genetics, Paris 🇫🇷
Project on selection on disease-associated variants, integrating ancient genomes, GWAS, and Ancestral Rec. Graphs.
Start May 2026 (flexible).
Apply here: emploi.cnrs.fr/Offres/CDD/U...
Project on selection on disease-associated variants, integrating ancient genomes, GWAS, and Ancestral Rec. Graphs.
Start May 2026 (flexible).
Apply here: emploi.cnrs.fr/Offres/CDD/U...
Portail Emploi CNRS - Offre d'emploi - Postdoc in human evolutionary genetics and bioinformatics (M/F)
Assurez-vous que votre profil candidat soit correctement renseigné avant de postuler
emploi.cnrs.fr
December 31, 2025 at 10:26 AM
📢 We're hiring a postdoc (33 months) in human evolutionary genomics / paleogenomics / statistical genetics, Paris 🇫🇷
Project on selection on disease-associated variants, integrating ancient genomes, GWAS, and Ancestral Rec. Graphs.
Start May 2026 (flexible).
Apply here: emploi.cnrs.fr/Offres/CDD/U...
Project on selection on disease-associated variants, integrating ancient genomes, GWAS, and Ancestral Rec. Graphs.
Start May 2026 (flexible).
Apply here: emploi.cnrs.fr/Offres/CDD/U...
Eviscerated son of a decapitated king and queen.
December 30, 2025 at 12:27 PM
Eviscerated son of a decapitated king and queen.
My New Year’s resolution is to finally catch up on the ones I made in 2001. I’m making steady progress on the pre-2000 resolutions by declaring their 25-year statute of limitations officially expired.
December 30, 2025 at 9:09 AM
My New Year’s resolution is to finally catch up on the ones I made in 2001. I’m making steady progress on the pre-2000 resolutions by declaring their 25-year statute of limitations officially expired.
Cold, sunny weekend in Metz. Layers of French–German history, cheerful streets, winter light, and the river. Christmas markets twinkling, vin chaud in hand, time slowing down nicely. A welcome break before an exciting science year ahead.
December 29, 2025 at 9:02 PM
Cold, sunny weekend in Metz. Layers of French–German history, cheerful streets, winter light, and the river. Christmas markets twinkling, vin chaud in hand, time slowing down nicely. A welcome break before an exciting science year ahead.
Reposted by Ishier Raote
#JobOffer
💼 Postdoc in human evolutionary genetics and bioinformatics (M/F)
📆 May 1st 2026
📍 Institut Jacques Monod / Peyrégne Lab
🔗 Apply on the CNRS job portal before January 9th emploi.cnrs.fr/Offres/CDD/U...
💼 Postdoc in human evolutionary genetics and bioinformatics (M/F)
📆 May 1st 2026
📍 Institut Jacques Monod / Peyrégne Lab
🔗 Apply on the CNRS job portal before January 9th emploi.cnrs.fr/Offres/CDD/U...
December 29, 2025 at 10:46 AM
#JobOffer
💼 Postdoc in human evolutionary genetics and bioinformatics (M/F)
📆 May 1st 2026
📍 Institut Jacques Monod / Peyrégne Lab
🔗 Apply on the CNRS job portal before January 9th emploi.cnrs.fr/Offres/CDD/U...
💼 Postdoc in human evolutionary genetics and bioinformatics (M/F)
📆 May 1st 2026
📍 Institut Jacques Monod / Peyrégne Lab
🔗 Apply on the CNRS job portal before January 9th emploi.cnrs.fr/Offres/CDD/U...
Season’s greetings to all! May your experiments be reproducible, your reviewers constructive, and your controls unambiguous.
December 24, 2025 at 7:07 PM
Season’s greetings to all! May your experiments be reproducible, your reviewers constructive, and your controls unambiguous.
Interesting preprint from the Boyce lab! Sec23IP (protein linking COPII inner and outer coats) is regulated by O-GlcNAcylation. Site-specific modification of its disordered domain controls O-GlcNAcylation, Sec31A recruitment, COPII assembly, and ER exit site function.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
December 22, 2025 at 12:23 PM
Interesting preprint from the Boyce lab! Sec23IP (protein linking COPII inner and outer coats) is regulated by O-GlcNAcylation. Site-specific modification of its disordered domain controls O-GlcNAcylation, Sec31A recruitment, COPII assembly, and ER exit site function.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Reposted by Ishier Raote
Thanks @jcellsci.bsky.social for this opportunity to contribute to your centenary collection with our take on the state of the field - 10 years after its modern reincarnation 🧪🌍
W/ @alebenoit.bsky.social @eelcotromer.bsky.social @fritzlaylin.bsky.social
journals.biologists.com/jcs/article/...
W/ @alebenoit.bsky.social @eelcotromer.bsky.social @fritzlaylin.bsky.social
journals.biologists.com/jcs/article/...
Evolutionary cell biology comes of age
Summary: This Perspective discusses how the discipline of evolutionary cell biology, by integrating evolutionary theory, comparative physiology and modern molecular approaches, works to understand how...
journals.biologists.com
December 19, 2025 at 4:13 PM
Thanks @jcellsci.bsky.social for this opportunity to contribute to your centenary collection with our take on the state of the field - 10 years after its modern reincarnation 🧪🌍
W/ @alebenoit.bsky.social @eelcotromer.bsky.social @fritzlaylin.bsky.social
journals.biologists.com/jcs/article/...
W/ @alebenoit.bsky.social @eelcotromer.bsky.social @fritzlaylin.bsky.social
journals.biologists.com/jcs/article/...
Reposted by Ishier Raote
Organelles do NOT have a single uniform pH.
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
Organelles harbour pH gradients https://www.biorxiv.org/content/10.64898/2025.12.12.694065v1
December 17, 2025 at 12:46 AM
Organelles do NOT have a single uniform pH.
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
A very exciting preprint from @krishnanyamuna.bsky.social shows that pH within organelles is not uniform: stable sub-organellar pH gradients exist even at the ~100nm scale, implying limited proton mobility - redefining organelle identity and regulation.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Organelles harbour pH gradients
Organelle pH is critical to organelle identity and function. Resident proteins that define each organelle modify transiting cargo proteins, with both retention and trafficking between organelles gover...
www.biorxiv.org
December 17, 2025 at 8:18 AM
A very exciting preprint from @krishnanyamuna.bsky.social shows that pH within organelles is not uniform: stable sub-organellar pH gradients exist even at the ~100nm scale, implying limited proton mobility - redefining organelle identity and regulation.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Reposted by Ishier Raote
@natcellbio.nature.com - Finally out! Skin cells mechanically protect their genome from UV by positioning melanin above nucleus. Keratin filaments & microtubules drive the process. Led by Silvia Benito-Martinez & @laurasalavessa.bsky.social @membramics-lab.bsky.social www.nature.com/articles/s41...
Keratin intermediate filaments mechanically position melanin pigments for genome photoprotection - Nature Cell Biology
Benito-Martínez, Salavessa and colleagues show that keratin intermediate filaments and microtubules control the three-dimensional perinuclear position of melanin-containing organelles, shielding the D...
www.nature.com
December 15, 2025 at 6:17 PM
@natcellbio.nature.com - Finally out! Skin cells mechanically protect their genome from UV by positioning melanin above nucleus. Keratin filaments & microtubules drive the process. Led by Silvia Benito-Martinez & @laurasalavessa.bsky.social @membramics-lab.bsky.social www.nature.com/articles/s41...
Reposted by Ishier Raote
My excellent collaborator, Giulia Zanetti, is advertising for a post-doc to work on our shared MRC grant. Please consider applying to this great lab with a supportive mentor to do some exciting structural biology.
www.ucl.ac.uk/work-at-ucl/...
www.ucl.ac.uk/work-at-ucl/...
UCL – University College London
UCL is consistently ranked as one of the top ten universities in the world (QS World University Rankings 2010-2022) and is No.2 in the UK for research power (Research Excellence Framework 2021).
www.ucl.ac.uk
December 11, 2025 at 12:53 PM
My excellent collaborator, Giulia Zanetti, is advertising for a post-doc to work on our shared MRC grant. Please consider applying to this great lab with a supportive mentor to do some exciting structural biology.
www.ucl.ac.uk/work-at-ucl/...
www.ucl.ac.uk/work-at-ucl/...
Reposted by Ishier Raote
TMEDs mediate versatile cargo transport in vesicle-dependent unconventional secretion url: rupress.org/jcb/article-...
TMEDs mediate versatile cargo transport in vesicle-dependent unconventional secretion
Zheng and colleagues uncover that p24/TMED family proteins mediate selective cargo translocation and release in vesicle-dependent UcPS. The switch of TMED
rupress.org
December 9, 2025 at 3:03 PM
TMEDs mediate versatile cargo transport in vesicle-dependent unconventional secretion url: rupress.org/jcb/article-...