Guillaume Gaullier
@guillawme.bsky.social
Structural biologist using #cryoEM ❄️🔬
I don't post here, follow https://fediscience.org/@Guillawme on the Fediverse.
If you must stay here, follow this bridge account: @Guillawme.fediscience.org.ap.brid.gy
But what will you do when Bsky "does a Twitter"?
I don't post here, follow https://fediscience.org/@Guillawme on the Fediverse.
If you must stay here, follow this bridge account: @Guillawme.fediscience.org.ap.brid.gy
But what will you do when Bsky "does a Twitter"?
Pinned
Guillaume Gaullier
@guillawme.bsky.social
· Oct 21
Guillaume Gaullier ❄️🔬 (@Guillawme@fediscience.org)
438 Posts, 188 Following, 151 Followers · Researcher from 🇫🇷 doing #cryoEM ❄️🔬 at Uppsala University 🇸🇪
Now working on cyanobacterial carboxysomes 🦠
Still interested in #nucleosome 🧬🙃🧬
Mostly science ...
fediscience.org
This is why these are my last posts here. But you still have two ways to connect with me:
1) Join the Fediverse! It's a bit more thinking to do upfront (which server to join?), but a more future-proof solution. I am fediscience.org/@Guillawme over there.
1) Join the Fediverse! It's a bit more thinking to do upfront (which server to join?), but a more future-proof solution. I am fediscience.org/@Guillawme over there.
Reposted by Guillaume Gaullier
ALC1 Finds a New Foothold on the Nucleosome's Super-Groove https://www.biorxiv.org/content/10.1101/2025.11.10.687450v1
November 11, 2025 at 2:48 AM
ALC1 Finds a New Foothold on the Nucleosome's Super-Groove https://www.biorxiv.org/content/10.1101/2025.11.10.687450v1
Reposted by Guillaume Gaullier
On a gruelling hunt for rare associated molecules in your #cryoEM particle stack and not sure where to look?
Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner!
guide.cryosparc.com/processing-d...
Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner!
guide.cryosparc.com/processing-d...
October 30, 2025 at 1:33 PM
On a gruelling hunt for rare associated molecules in your #cryoEM particle stack and not sure where to look?
Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner!
guide.cryosparc.com/processing-d...
Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner!
guide.cryosparc.com/processing-d...
Reposted by Guillaume Gaullier
ALC1 Finds a New Foothold on the Nucleosome's Super-Groove https://www.biorxiv.org/content/10.1101/2025.11.10.687450v1
November 11, 2025 at 2:48 AM
ALC1 Finds a New Foothold on the Nucleosome's Super-Groove https://www.biorxiv.org/content/10.1101/2025.11.10.687450v1
Reposted by Guillaume Gaullier
Announcing cryo-EM heterogeneity challenge #2, now dubbed the 2025 Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms (CAHRA)! Join us for a webinar next Friday (Nov 14th) to learn more. Datasets already posted here: heterogeneity.notion.site/challenge #cryoem
CAHRA 2025: Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms
A community-wide data processing challenge for cryo-electron microscopy.
heterogeneity.notion.site
November 7, 2025 at 10:45 PM
Announcing cryo-EM heterogeneity challenge #2, now dubbed the 2025 Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms (CAHRA)! Join us for a webinar next Friday (Nov 14th) to learn more. Datasets already posted here: heterogeneity.notion.site/challenge #cryoem
Reposted by Guillaume Gaullier
If you ever thought science was too tame a career, how does kite skiing 4,000km across Antarctica over 3 months sound?
#antarctica #adventure #expedition #science #climatechange
#antarctica #adventure #expedition #science #climatechange
November 2, 2025 at 10:14 AM
If you ever thought science was too tame a career, how does kite skiing 4,000km across Antarctica over 3 months sound?
#antarctica #adventure #expedition #science #climatechange
#antarctica #adventure #expedition #science #climatechange
Reposted by Guillaume Gaullier
HHMI adopts Plan U journals.plos.org/plosbiology/...
September 24, 2025 at 6:08 PM
HHMI adopts Plan U journals.plos.org/plosbiology/...
@nucleosomepolice.bsky.social do you think this newly discovered giant virus has nucleosomes? doi.org/10.1038/s414...
Naiavirus: an enveloped giant virus with a pleomorphic, flexible tail - Nature Communications
Rodrigues and Queiroz et al. report the discovery of Naiavirus, the largest enveloped virus, isolated from a Brazil biome. With an oval capsid and flexible tail, its giant genome holds many novel gene...
doi.org
September 18, 2025 at 7:57 AM
@nucleosomepolice.bsky.social do you think this newly discovered giant virus has nucleosomes? doi.org/10.1038/s414...
Reposted by Guillaume Gaullier
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
September 13, 2025 at 12:05 AM
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
Reposted by Guillaume Gaullier
Advanced #cryoEM workflows are easy in #CryoSPARC: use the Low-Level Results Interface to combine upstream extracted particles with downstream pose and CTF information, use a volume result from an intermediate iteration of a refinement to create a mask, and more! guide.cryosparc.com/application-...
September 10, 2025 at 3:46 PM
Advanced #cryoEM workflows are easy in #CryoSPARC: use the Low-Level Results Interface to combine upstream extracted particles with downstream pose and CTF information, use a volume result from an intermediate iteration of a refinement to create a mask, and more! guide.cryosparc.com/application-...
Reposted by Guillaume Gaullier
Great point. Make it opt out, not opt in, for authors to see feedback.
We need to grow a culture of rapid and constructive feedback to replace ‘peer review’… which is outdated, was not designed and is not fit for purpose.
How can we do that if public reviews are not even seen by the authors?
We need to grow a culture of rapid and constructive feedback to replace ‘peer review’… which is outdated, was not designed and is not fit for purpose.
How can we do that if public reviews are not even seen by the authors?
September 8, 2025 at 8:07 PM
Great point. Make it opt out, not opt in, for authors to see feedback.
We need to grow a culture of rapid and constructive feedback to replace ‘peer review’… which is outdated, was not designed and is not fit for purpose.
How can we do that if public reviews are not even seen by the authors?
We need to grow a culture of rapid and constructive feedback to replace ‘peer review’… which is outdated, was not designed and is not fit for purpose.
How can we do that if public reviews are not even seen by the authors?
Reposted by Guillaume Gaullier
Why hasn't Preprint Peer Review caught on more?
I discovered a small, but simple reason - authors are missing out on the JOY of getting feedback because they aren't notified when feedback occurs.
more in thread below!
I discovered a small, but simple reason - authors are missing out on the JOY of getting feedback because they aren't notified when feedback occurs.
more in thread below!
September 8, 2025 at 3:59 PM
Why hasn't Preprint Peer Review caught on more?
I discovered a small, but simple reason - authors are missing out on the JOY of getting feedback because they aren't notified when feedback occurs.
more in thread below!
I discovered a small, but simple reason - authors are missing out on the JOY of getting feedback because they aren't notified when feedback occurs.
more in thread below!
Reposted by Guillaume Gaullier
Thanks for sharing @olibclarke.bsky.social. I wonder if many more "hollow" complexes are co-purified with binding-partners. Let's all put more datasets on EMPIAR to revisit processing with new creative approaches like Oli's.
September 1, 2025 at 4:50 PM
Thanks for sharing @olibclarke.bsky.social. I wonder if many more "hollow" complexes are co-purified with binding-partners. Let's all put more datasets on EMPIAR to revisit processing with new creative approaches like Oli's.
Reposted by Guillaume Gaullier
In the Bay Area? Love computational cryo-EM/ET?
Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community!
Details and registration at frostbyte-cryoem.github.io
Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!
Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community!
Details and registration at frostbyte-cryoem.github.io
Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!
May 12, 2025 at 8:01 PM
In the Bay Area? Love computational cryo-EM/ET?
Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community!
Details and registration at frostbyte-cryoem.github.io
Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!
Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community!
Details and registration at frostbyte-cryoem.github.io
Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!
Reposted by Guillaume Gaullier
Come join us as a postdoc! We have an open position in biochemistry of cyanobacterial CO2 fixation focusing on regulation of carboxysome function! Please help me spread the word and welcome to apply! www.blikstadlab.org/open-positio...
Open positions
Postdoctoral scholar in biochemistry of cyanobacterial CO 2 fixation The Blikstad research group is searching for a postdoc to study fundamental principles of CO 2 fixation in cyanobacteria. Our...
www.blikstadlab.org
April 16, 2025 at 10:54 AM
Come join us as a postdoc! We have an open position in biochemistry of cyanobacterial CO2 fixation focusing on regulation of carboxysome function! Please help me spread the word and welcome to apply! www.blikstadlab.org/open-positio...
Reposted by Guillaume Gaullier
Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍
Preprint📜: www.biorxiv.org/content/10.1...
A short thread🧵👇
Preprint📜: www.biorxiv.org/content/10.1...
A short thread🧵👇
January 6, 2025 at 11:19 AM
Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍
Preprint📜: www.biorxiv.org/content/10.1...
A short thread🧵👇
Preprint📜: www.biorxiv.org/content/10.1...
A short thread🧵👇
Reposted by Guillaume Gaullier
Is it me or is this place really taking off?
Shall we all take a step closer to full sustainable independence and try Mastodon too!
Shall we all take a step closer to full sustainable independence and try Mastodon too!
November 8, 2024 at 6:40 PM
Is it me or is this place really taking off?
Shall we all take a step closer to full sustainable independence and try Mastodon too!
Shall we all take a step closer to full sustainable independence and try Mastodon too!
Since the beginning of Bsky I've been skeptical it's a good replacement for Twitter. It's already taken investor money, and when the return on investment comes due, we'll get either: ads, paid accounts, sell-out to some richer tech bro. Or a combination of these unpleasant outcomes.
October 21, 2024 at 10:05 AM
Since the beginning of Bsky I've been skeptical it's a good replacement for Twitter. It's already taken investor money, and when the return on investment comes due, we'll get either: ads, paid accounts, sell-out to some richer tech bro. Or a combination of these unpleasant outcomes.
Reposted by Guillaume Gaullier
My fabulous postdoc worked SO hard on a fellowship application (submitted today) and I am so proud of them. y'all reviewers, BE KIND and if you must, offer criticms that is at least constructive! Consider that there are differences in life experiences. Not everybody has a straight trajectory. 🧪✍️
March 31, 2024 at 2:51 PM
My fabulous postdoc worked SO hard on a fellowship application (submitted today) and I am so proud of them. y'all reviewers, BE KIND and if you must, offer criticms that is at least constructive! Consider that there are differences in life experiences. Not everybody has a straight trajectory. 🧪✍️
Summary of this new paper 🧬🧶 on Mastodon: fediscience.org/@Guillawme/1...
February 18, 2024 at 10:14 PM
Summary of this new paper 🧬🧶 on Mastodon: fediscience.org/@Guillawme/1...
Summary of this 2021 paper 🧬🧶 on Mastodon (I wrote it before bsky was out of invitation-only, I have no motivation to copy/paste it all here, and I still think Mastodon is a more sustainable platform than bsky anyway): fediscience.org/@Guillawme/1...
February 18, 2024 at 10:14 PM
Summary of this 2021 paper 🧬🧶 on Mastodon (I wrote it before bsky was out of invitation-only, I have no motivation to copy/paste it all here, and I still think Mastodon is a more sustainable platform than bsky anyway): fediscience.org/@Guillawme/1...
"Is X-ray crystallography dead?" some asked when cryoEM got momentum. Depends on what you work on. For nucleosomes it certainly looks like it is dead: all structures with nucleosomes released in 2023 were cryoEM structures! @nucleosomepolice.bsky.social
guillawme.github.io/insights-fro...
guillawme.github.io/insights-fro...
February 11, 2024 at 10:20 PM
"Is X-ray crystallography dead?" some asked when cryoEM got momentum. Depends on what you work on. For nucleosomes it certainly looks like it is dead: all structures with nucleosomes released in 2023 were cryoEM structures! @nucleosomepolice.bsky.social
guillawme.github.io/insights-fro...
guillawme.github.io/insights-fro...
New paper published! 😊
Nucleosomes, ADP-ribosylation, a chromatin remodeler, smFRET, cryoEM. And the best of all: it confirmed a hypothesis we posited in our previous paper in 2021! Super nice to finally see this new paper out. 🤩 🧬🧶
doi.org/10.1038/s414...
Nucleosomes, ADP-ribosylation, a chromatin remodeler, smFRET, cryoEM. And the best of all: it confirmed a hypothesis we posited in our previous paper in 2021! Super nice to finally see this new paper out. 🤩 🧬🧶
doi.org/10.1038/s414...
Asymmetric nucleosome PARylation at DNA breaks mediates directional nucleosome sliding by ALC1 - Nat...
Bacic et al. demonstrate that PARP1/HPF1 preferentially modify histone tails closest to the DNA break, directing ALC1-catalyzed nucleosome sliding. These findings suggest a mechanism for rendering DNA...
doi.org
February 5, 2024 at 9:49 PM
New paper published! 😊
Nucleosomes, ADP-ribosylation, a chromatin remodeler, smFRET, cryoEM. And the best of all: it confirmed a hypothesis we posited in our previous paper in 2021! Super nice to finally see this new paper out. 🤩 🧬🧶
doi.org/10.1038/s414...
Nucleosomes, ADP-ribosylation, a chromatin remodeler, smFRET, cryoEM. And the best of all: it confirmed a hypothesis we posited in our previous paper in 2021! Super nice to finally see this new paper out. 🤩 🧬🧶
doi.org/10.1038/s414...
Reposted by Guillaume Gaullier
What about cross-posting on mastodon? It's not a closed community etc etc.
I'm only here because sci-twitter seems to have migrated here.
I think mastodon is a much better solution than the options brought to you by the letters X, B, T, or S.
I'm only here because sci-twitter seems to have migrated here.
I think mastodon is a much better solution than the options brought to you by the letters X, B, T, or S.
September 24, 2023 at 12:37 AM
What about cross-posting on mastodon? It's not a closed community etc etc.
I'm only here because sci-twitter seems to have migrated here.
I think mastodon is a much better solution than the options brought to you by the letters X, B, T, or S.
I'm only here because sci-twitter seems to have migrated here.
I think mastodon is a much better solution than the options brought to you by the letters X, B, T, or S.