Dimitrios L. Wagner
@dlwagner.bsky.social
Physician & science enthusiast @BCMHouston @ChariteBerlin @BIHregeneration // Genome Editing and Immunology // 50% of @immunoboys.bsky.social / @dlwagnerlabs.bsky.social
Pinned
Dimitrios L. Wagner
@dlwagner.bsky.social
· Sep 19
Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases
Current gene transfer methods often lack the precision, versatility, or efficiency when integrating large transgenes, limiting the ability to engineer therapeutic T-cells with more complex payloads. H...
www.biorxiv.org
You like engineering T cells, but you are missing the right flavor?
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Reposted by Dimitrios L. Wagner
Rotation PhD student @emiliedevet.bsky.social in our lab made the Dish soap protocol work (on the first attempt) for a nuclear target with Dawn (the US version of dreft/fairy ) and thereby extended the dish soap protocol to a version for across the atlantic 🤩 @tothovalab.bsky.social #hemesky
January 21, 2026 at 7:40 PM
Rotation PhD student @emiliedevet.bsky.social in our lab made the Dish soap protocol work (on the first attempt) for a nuclear target with Dawn (the US version of dreft/fairy ) and thereby extended the dish soap protocol to a version for across the atlantic 🤩 @tothovalab.bsky.social #hemesky
Reposted by Dimitrios L. Wagner
Distinct EBV-Associated Phenotypes Due to a Novel Homozygous Missense Variant in CD27. https://www.biorxiv.org/content/10.64898/2026.01.15.698722v1
January 16, 2026 at 2:16 AM
Distinct EBV-Associated Phenotypes Due to a Novel Homozygous Missense Variant in CD27. https://www.biorxiv.org/content/10.64898/2026.01.15.698722v1
Reposted by Dimitrios L. Wagner
Together with @ronghuizhu.bsky.social, we are thrilled to present our new perturb-seq study of 22M primary CD4+ T cells, across donors and timepoints – the result of a decade-long collaboration between the Marson @marsonlab.bsky.social and Pritchard @jkpritch.bsky.social labs 🧵 tinyurl.com/gwt2025
Genome-scale perturb-seq in primary human CD4+ T cells maps context-specific regulators of T cell programs and human immune traits
Gene regulatory networks encode the fundamental logic of cellular functions, but systematic network mapping remains challenging, especially in cell states relevant to human biology and disease. Here, ...
tinyurl.com
January 5, 2026 at 6:42 PM
Together with @ronghuizhu.bsky.social, we are thrilled to present our new perturb-seq study of 22M primary CD4+ T cells, across donors and timepoints – the result of a decade-long collaboration between the Marson @marsonlab.bsky.social and Pritchard @jkpritch.bsky.social labs 🧵 tinyurl.com/gwt2025
Reposted by Dimitrios L. Wagner
Terrific to have our paper, led by @tjflemin.bsky.social, featured as a plenary article in @bloodjournal.bsky.social today: ashpublications.org/blood/articl...
December 18, 2025 at 6:01 PM
Terrific to have our paper, led by @tjflemin.bsky.social, featured as a plenary article in @bloodjournal.bsky.social today: ashpublications.org/blood/articl...
Reposted by Dimitrios L. Wagner
🚨This week in @natcomms.nature.com: We resolve an enduring enigma in T cell biology—how TCR binding to p/HLA triggers intracellular signaling. CryoEM in native environment reveals a 'jack-in-the-box' mechanism! Collab btw
@rockefeller.edu @mskcancercenter.bsky.social
rdcu.be/eUPPl
@rockefeller.edu @mskcancercenter.bsky.social
rdcu.be/eUPPl
The resting and ligand-bound states of the membrane-embedded human T-cell receptor–CD3 complex
Nature Communications - The T-cell receptor (TCR) activation mechanism has remained uncertain. Here, the authors present molecular structures for the apo and ligand-bound human TCR–CD3...
rdcu.be
December 18, 2025 at 6:44 PM
🚨This week in @natcomms.nature.com: We resolve an enduring enigma in T cell biology—how TCR binding to p/HLA triggers intracellular signaling. CryoEM in native environment reveals a 'jack-in-the-box' mechanism! Collab btw
@rockefeller.edu @mskcancercenter.bsky.social
rdcu.be/eUPPl
@rockefeller.edu @mskcancercenter.bsky.social
rdcu.be/eUPPl
Reposted by Dimitrios L. Wagner
Clinical development of gene edited tacrolimus-resistant Treg (FKBP12KO-Treg) to enable simultaneous immunosuppression and support of immune regulation https://www.biorxiv.org/content/10.64898/2025.12.10.693159v1
December 13, 2025 at 10:16 PM
Clinical development of gene edited tacrolimus-resistant Treg (FKBP12KO-Treg) to enable simultaneous immunosuppression and support of immune regulation https://www.biorxiv.org/content/10.64898/2025.12.10.693159v1
Reposted by Dimitrios L. Wagner
Original work here ⬇️, led by the inimitable Lorenzo Brunetti and Michael Gundry. This work became foundational in the field.
Mutant NPM1 Maintains the Leukemic State through HOX Expression
Brunetti et al. show that specific loss of NPM1c from the cytoplasm leads to downregulation
of HOX genes and differentiation in NPM1 mutant AML. Blocking NPM1c nuclear export
by XPO1 inhibition reduce...
www.cell.com
December 12, 2025 at 12:03 AM
Original work here ⬇️, led by the inimitable Lorenzo Brunetti and Michael Gundry. This work became foundational in the field.
Reposted by Dimitrios L. Wagner
Today's the print release of a #RibackLab and #GoodellLab collaboration— we’re excited to share the visual story behind the science, including the dramatic artwork that inspired how we thought about aberrant function of NPM1c - which occurs in about 1 in 3 adult acute myeloid leukemias (#AML).
December 12, 2025 at 12:03 AM
Today's the print release of a #RibackLab and #GoodellLab collaboration— we’re excited to share the visual story behind the science, including the dramatic artwork that inspired how we thought about aberrant function of NPM1c - which occurs in about 1 in 3 adult acute myeloid leukemias (#AML).
Reposted by Dimitrios L. Wagner
A big advance, folks, for off-the-shelf, universal, engineered (base-edited) T cells achieving complete response vs incurable leukemia in several patients
www.nejm.org/doi/full/10....
www.nejm.org/doi/full/10....
December 8, 2025 at 10:10 PM
A big advance, folks, for off-the-shelf, universal, engineered (base-edited) T cells achieving complete response vs incurable leukemia in several patients
www.nejm.org/doi/full/10....
www.nejm.org/doi/full/10....
Reposted by Dimitrios L. Wagner
Our case report of a patient developing drug-induced systemic sclerosis after bleomycin exposure – still showing antibodies after 5 years. Nicely done Philipp 🎉 #rheumsky
authors.elsevier.com/sd/article/S...
authors.elsevier.com/sd/article/S...
December 6, 2025 at 10:47 PM
Our case report of a patient developing drug-induced systemic sclerosis after bleomycin exposure – still showing antibodies after 5 years. Nicely done Philipp 🎉 #rheumsky
authors.elsevier.com/sd/article/S...
authors.elsevier.com/sd/article/S...
Reposted by Dimitrios L. Wagner
Extrusive and cohesive cohesin cooperate to repair double-strand breaks in DNA @science.org @albertomarin.bsky.social @gerlichlab.bsky.social @imbavienna.bsky.social
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
December 5, 2025 at 3:28 AM
Extrusive and cohesive cohesin cooperate to repair double-strand breaks in DNA @science.org @albertomarin.bsky.social @gerlichlab.bsky.social @imbavienna.bsky.social
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Dimitrios L. Wagner
Big news! Our team developed Neuro-PERC, a non-viral CRISPR delivery platform for genome editing of the brain. In our new preprint - bit.ly/NeuroPERC_pre - we report efficient editing in multiple models & species, including field-first results in large animals (pigs) 🧵👇
bit.ly
December 2, 2025 at 8:59 AM
Big news! Our team developed Neuro-PERC, a non-viral CRISPR delivery platform for genome editing of the brain. In our new preprint - bit.ly/NeuroPERC_pre - we report efficient editing in multiple models & species, including field-first results in large animals (pigs) 🧵👇
Reposted by Dimitrios L. Wagner
On this #WorldAIDSday @cgaebler.bsky.social, Olaf Penack & teams at #CharitéBerlin report the #SecondBerlinPatient cured of #HIV after heterozygous CCR5Δ32 HSCT. The study opens exciting perspectives for immune-based #HIVCure strategies for longterm ART-free viral control and ultimately cure.
Sustained HIV-1 remission after heterozygous CCR5Δ32 stem cell transplantation - Nature
Nature - Sustained HIV-1 remission after heterozygous CCR5Δ32 stem cell transplantation
www.nature.com
December 1, 2025 at 9:48 PM
On this #WorldAIDSday @cgaebler.bsky.social, Olaf Penack & teams at #CharitéBerlin report the #SecondBerlinPatient cured of #HIV after heterozygous CCR5Δ32 HSCT. The study opens exciting perspectives for immune-based #HIVCure strategies for longterm ART-free viral control and ultimately cure.
Reposted by Dimitrios L. Wagner
November 24, 2025 at 11:46 PM
Reposted by Dimitrios L. Wagner
Adapting the 10x workflow to detect EBV reads, Younis et al show that EBV-transcribing B cells are increased in SLE patients, especially among CD27+CD21low B cells. They have autoreactive BCRs and are more poised for antigen-presentation.
2025 Sci Transl Med 🧪 ⑂
www.science.org/doi/10.1126/...
2025 Sci Transl Med 🧪 ⑂
www.science.org/doi/10.1126/...
November 13, 2025 at 10:48 PM
Adapting the 10x workflow to detect EBV reads, Younis et al show that EBV-transcribing B cells are increased in SLE patients, especially among CD27+CD21low B cells. They have autoreactive BCRs and are more poised for antigen-presentation.
2025 Sci Transl Med 🧪 ⑂
www.science.org/doi/10.1126/...
2025 Sci Transl Med 🧪 ⑂
www.science.org/doi/10.1126/...
Reposted by Dimitrios L. Wagner
What mechanisms drive checkpoint inhibitor-associated interstitial nephritis (ICI-AIN)?
In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13.
www.biorxiv.org/content/10.1...
1/n
#Nephsky 🧪
In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13.
www.biorxiv.org/content/10.1...
1/n
#Nephsky 🧪
November 5, 2025 at 6:24 PM
What mechanisms drive checkpoint inhibitor-associated interstitial nephritis (ICI-AIN)?
In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13.
www.biorxiv.org/content/10.1...
1/n
#Nephsky 🧪
In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13.
www.biorxiv.org/content/10.1...
1/n
#Nephsky 🧪
Reposted by Dimitrios L. Wagner
A monoclonal antibody that inhibits the shedding of CD16a and CD16b and promotes antibody-dependent cellular cytotoxicity against tumors @natcomms.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
November 12, 2025 at 12:56 AM
A monoclonal antibody that inhibits the shedding of CD16a and CD16b and promotes antibody-dependent cellular cytotoxicity against tumors @natcomms.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Dimitrios L. Wagner
(1/10) How do diverse leukemia mutations converge on the same molecular program? In #RibackLab first manuscript @cp-cell.bsky.social, collaboration with @goodell-lab.bsky.social shows that disparate mutations rewire shared protein networks to form nuclear condensates called C-bodies.
November 4, 2025 at 5:58 PM
(1/10) How do diverse leukemia mutations converge on the same molecular program? In #RibackLab first manuscript @cp-cell.bsky.social, collaboration with @goodell-lab.bsky.social shows that disparate mutations rewire shared protein networks to form nuclear condensates called C-bodies.
Reposted by Dimitrios L. Wagner
Ubiquitinylation is more complex than just a signal for proteasomal degradation: Linear ubiquitinylation of Methionine-1 is important for NFkB signalling.
LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop.
🧪 ⑂
1/2
LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop.
🧪 ⑂
1/2
October 20, 2025 at 7:51 PM
Ubiquitinylation is more complex than just a signal for proteasomal degradation: Linear ubiquitinylation of Methionine-1 is important for NFkB signalling.
LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop.
🧪 ⑂
1/2
LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop.
🧪 ⑂
1/2
Reposted by Dimitrios L. Wagner
Ever noticed that #CRISPR editing results differ between cells? Awesome PhD student Moritz Schlapansky developed "scOUT-seq" to measure single cell transcriptomes + editing. 1.2 million cells, 74 cell types, living 🐭. Cell subtypes differ wildly from bulk average! www.biorxiv.org/content/10.1...
Cell-stereotyped DNA repair outcomes are widespread during genome editing
Genome editing outcomes are governed by DNA repair pathways that vary with cell type and state. We developed scOUT-seq (single-cell Outcomes Using Transcript sequencing), a scalable approach that join...
www.biorxiv.org
October 24, 2025 at 8:44 AM
Ever noticed that #CRISPR editing results differ between cells? Awesome PhD student Moritz Schlapansky developed "scOUT-seq" to measure single cell transcriptomes + editing. 1.2 million cells, 74 cell types, living 🐭. Cell subtypes differ wildly from bulk average! www.biorxiv.org/content/10.1...
Reposted by Dimitrios L. Wagner
A new subspecialty takes off! Astroimmunology is focused on the effects of #spaceflight on the #immunesystem and defines avenues for countermeasures. A bonus: the work will also inform research on #aging @natrevimmunol.nature.com evimmunol.nature.com www.buckinstitute.org/news/a-hitch...
A hitchhiker’s guide to the galaxy of space immunology
Buck scientists help define the scope of Astroimmunology With the advent of commercial spaceflight, an increasing number of people may be heading into space in the coming years. Some will even get ...
www.buckinstitute.org
October 16, 2025 at 4:20 PM
A new subspecialty takes off! Astroimmunology is focused on the effects of #spaceflight on the #immunesystem and defines avenues for countermeasures. A bonus: the work will also inform research on #aging @natrevimmunol.nature.com evimmunol.nature.com www.buckinstitute.org/news/a-hitch...
Reposted by Dimitrios L. Wagner
BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)
October 15, 2025 at 12:22 PM
BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)
Reposted by Dimitrios L. Wagner
What is a promoter? And how does it work?
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
Enhancer-promoter compatibility is mediated by the promoter-proximal region
Gene promoters induce transcription in response to distal enhancers. How enhancers specifically activate their target promoter while bypassing other promoters remains unclear. Here, we find that the p...
www.biorxiv.org
October 16, 2025 at 3:06 PM
What is a promoter? And how does it work?
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
Reposted by Dimitrios L. Wagner
Honored to be part of the 2025 #STATWunderkinds! Grateful for the many supportive mentors, colleagues, and friends with special shoutout to @bkleinstiver.bsky.social.
Full list: www.statnews.com/wunderkinds/
@statnews.com @mitdeptofbe.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social
Full list: www.statnews.com/wunderkinds/
@statnews.com @mitdeptofbe.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social
October 15, 2025 at 11:55 AM
Honored to be part of the 2025 #STATWunderkinds! Grateful for the many supportive mentors, colleagues, and friends with special shoutout to @bkleinstiver.bsky.social.
Full list: www.statnews.com/wunderkinds/
@statnews.com @mitdeptofbe.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social
Full list: www.statnews.com/wunderkinds/
@statnews.com @mitdeptofbe.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social
“ ‘Off the shelf’ Treg may soon be… on the shelf. “ - awesome quote by Prof. Fadi Issa.
Check out our new story: 👇
www.nature.com/articles/s41...
One week after the Nobel prize for Treg 🍀
Check out our new story: 👇
www.nature.com/articles/s41...
One week after the Nobel prize for Treg 🍀
October 13, 2025 at 11:09 PM
“ ‘Off the shelf’ Treg may soon be… on the shelf. “ - awesome quote by Prof. Fadi Issa.
Check out our new story: 👇
www.nature.com/articles/s41...
One week after the Nobel prize for Treg 🍀
Check out our new story: 👇
www.nature.com/articles/s41...
One week after the Nobel prize for Treg 🍀