Dorien Pastoors
@dpastoors.bsky.social
Postdoc at DFCI, Boston, USA | molecular biology & hematology 🧫 | data visualization in #ggplot | swimming, running, baking | HemeSky feed
Pinned
CEBPA repression by MECOM blocks differentiation to drive aggressive leukemias
Key PointsMECOM promotes malignant stem cell–like states in aggressive AMLs by directly repressing prodifferentiation gene regulatory programs.A MECOM-boun
ashpublications.org
Thanks to all the co-authors - @delwelruud.bsky.social ! This paper was accompanied by a paper from @tjflemin.bsky.social and @bloodgenes.bsky.social with similar conclusions and very cool dCas9 experiments: ashpublications.org/blood/articl...
Reposted by Dorien Pastoors
Preprint alert: Jiangyuan Liu developed a new workflow for chromatin loop calling across Hi-C datasets, e.g., during differentiation. Most loops are shared between datasets/cell states. Important work for all interested in chromatin loops and how to identify them!
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
www.biorxiv.org
January 21, 2026 at 11:20 AM
Preprint alert: Jiangyuan Liu developed a new workflow for chromatin loop calling across Hi-C datasets, e.g., during differentiation. Most loops are shared between datasets/cell states. Important work for all interested in chromatin loops and how to identify them!
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Rotation PhD student @emiliedevet.bsky.social in our lab made the Dish soap protocol work (on the first attempt) for a nuclear target with Dawn (the US version of dreft/fairy ) and thereby extended the dish soap protocol to a version for across the atlantic 🤩 @tothovalab.bsky.social #hemesky
January 21, 2026 at 7:40 PM
Rotation PhD student @emiliedevet.bsky.social in our lab made the Dish soap protocol work (on the first attempt) for a nuclear target with Dawn (the US version of dreft/fairy ) and thereby extended the dish soap protocol to a version for across the atlantic 🤩 @tothovalab.bsky.social #hemesky
Reposted by Dorien Pastoors
Happy to share Joey (Zhuoyi)'s review on technologies to map RBP:RNA and (especially) RBP-associated RNA:RNA interactions is now up at RNA! pubmed.ncbi.nlm.nih.gov/41535089
Progress and challenges in profiling protein-RNA and protein-associated RNA-RNA interactions - PubMed
RNA binding proteins (RBPs) play essential roles in post-transcriptional gene regulation by interacting with a wide range of RNA targets. In addition to regulating RNA processing via individual RBP-RNA interactions, there is a growing appreciation of the regulatory impact of protein-associated RNA-R …
pubmed.ncbi.nlm.nih.gov
January 18, 2026 at 3:29 AM
Happy to share Joey (Zhuoyi)'s review on technologies to map RBP:RNA and (especially) RBP-associated RNA:RNA interactions is now up at RNA! pubmed.ncbi.nlm.nih.gov/41535089
Thanks Vijay!! Congratulations to your manuscript too - really cool to see that it's out!
Please also see a terrific companion paper from @dpastoors.bsky.social and @delwelruud.bsky.social: ashpublications.org/blood/articl... + a great commentary from @bertiegottgens.bsky.social and Wilson: ashpublications.org/blood/articl...
Breaking the silence: restoring CEBPA to fight leukemia
During the initial identification of leukemia oncogenes in the 1980s and 1990s, the molecular biology revolution was widely expected to deliver intuitive r
ashpublications.org
January 6, 2026 at 5:16 PM
Thanks Vijay!! Congratulations to your manuscript too - really cool to see that it's out!
How do single transcription factors prevent lineage differentiation?
In this issue of Blood, the second paper of my PhD is published. We show that MECOM, a key HSC TF, maintains stem cell state by preventing change through repression of CEBPA. #hemesky ashpublications.org/blood/articl...
In this issue of Blood, the second paper of my PhD is published. We show that MECOM, a key HSC TF, maintains stem cell state by preventing change through repression of CEBPA. #hemesky ashpublications.org/blood/articl...
January 6, 2026 at 5:13 PM
How do single transcription factors prevent lineage differentiation?
In this issue of Blood, the second paper of my PhD is published. We show that MECOM, a key HSC TF, maintains stem cell state by preventing change through repression of CEBPA. #hemesky ashpublications.org/blood/articl...
In this issue of Blood, the second paper of my PhD is published. We show that MECOM, a key HSC TF, maintains stem cell state by preventing change through repression of CEBPA. #hemesky ashpublications.org/blood/articl...
Reposted by Dorien Pastoors
For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper?
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
currentprotocols.onlinelibrary.wiley.com
September 19, 2025 at 2:39 PM
For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper?
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
Excited to share that I started as a Postdoctoral Fellow in @tothovalab.bsky.social at @danafarber.bsky.social in Boston! Looking forward to the next years of molecular & myeloid biology in Boston 🤩🌇
September 20, 2025 at 2:05 AM
Excited to share that I started as a Postdoctoral Fellow in @tothovalab.bsky.social at @danafarber.bsky.social in Boston! Looking forward to the next years of molecular & myeloid biology in Boston 🤩🌇
Yesterday I successfully defended my PhD thesis!!! It was a fantastic day where I got to celebrate with many colleagues, friends and family (this smile was on my face all day long)
June 27, 2025 at 5:56 PM
Yesterday I successfully defended my PhD thesis!!! It was a fantastic day where I got to celebrate with many colleagues, friends and family (this smile was on my face all day long)
Thanks for the opportunity to present our work at the last day of #EHA2025 🤩 on MECOM as a repressor of CEBPA - also see our recent preprint: www.biorxiv.org/content/10.1... and I very much hope you can stay tuned for the final version soon-ish) #erasmusmcblood #hemesky
June 15, 2025 at 8:34 PM
Thanks for the opportunity to present our work at the last day of #EHA2025 🤩 on MECOM as a repressor of CEBPA - also see our recent preprint: www.biorxiv.org/content/10.1... and I very much hope you can stay tuned for the final version soon-ish) #erasmusmcblood #hemesky
My HemeSky feed is now also picking up #EHA2025! bsky.app/profile/dpas...
June 14, 2025 at 6:56 AM
My HemeSky feed is now also picking up #EHA2025! bsky.app/profile/dpas...
🤣 🔥
Sweaty or not, here we go day 2! The program is 🤩🤩🤩 today.
#EHA2025.
#EHA2025.
a woman sitting in a chair with the words melting behind her
ALT: a woman sitting in a chair with the words melting behind her
media.tenor.com
June 13, 2025 at 2:23 PM
🤣 🔥
Check out poster 1115 on MBD4 deficiency to model clonal hematopoeisis ⬇️⬇️ #EHA2025 🩸🧪
June 13, 2025 at 2:21 PM
Check out poster 1115 on MBD4 deficiency to model clonal hematopoeisis ⬇️⬇️ #EHA2025 🩸🧪
Congratulations!! 🧪🩸
Well deserved recognition for team Clotterdam's very own @mandylauw.bsky.social #YoungEHA Congratulations Mandy!
We are HemaSphere proud at #EHA2025, where 2 of our editors received special EHA awards! Congrats to Jan Cools, Ed in Chief, for the education & mentoring award; and to Mandy Lauw, Scientific Ed, for the YoungEHA award. Well deserved 👏 @jancools.bsky.social @mandylauw.bsky.social
June 13, 2025 at 8:29 AM
Congratulations!! 🧪🩸
Really enjoying #EHA2025 so far. And also how many people list there BlueSky as social media!! Posts are collected on this HemeSky feed: 🩸🧪https://bsky.app/profile/dpastoors.bsky.social/feed/aaagr4h5mq62a
June 13, 2025 at 8:29 AM
Really enjoying #EHA2025 so far. And also how many people list there BlueSky as social media!! Posts are collected on this HemeSky feed: 🩸🧪https://bsky.app/profile/dpastoors.bsky.social/feed/aaagr4h5mq62a
Spotted fellow #erasmusmcblood PhD candidate @shordijk.bsky.social at the #EHA wall of fame for her Mobility grant 🤩🤩 Congratulations !! @clotterdam.bsky.social
June 12, 2025 at 10:49 AM
Spotted fellow #erasmusmcblood PhD candidate @shordijk.bsky.social at the #EHA wall of fame for her Mobility grant 🤩🤩 Congratulations !! @clotterdam.bsky.social
SUPER excited to share that I got my PhD thesis - thanks to a special delivery from @massaarsanne.bsky.social to Milan!! On the 26th of June, I will defend my thesis at 15:30 CET. You can follow online at eur.cloud.panopto.eu/Panopto/Page... #hemesky
June 8, 2025 at 1:08 PM
SUPER excited to share that I got my PhD thesis - thanks to a special delivery from @massaarsanne.bsky.social to Milan!! On the 26th of June, I will defend my thesis at 15:30 CET. You can follow online at eur.cloud.panopto.eu/Panopto/Page... #hemesky
This Thursday, I will be part of the @oncodeinstitute.bsky.social team to cycle up the Alpe d'Huez - a (very steep!!!!) mountain in the French Alps - to raise money for the Dutch Cancer Society @liting-chen.bsky.social
June 3, 2025 at 4:32 PM
This Thursday, I will be part of the @oncodeinstitute.bsky.social team to cycle up the Alpe d'Huez - a (very steep!!!!) mountain in the French Alps - to raise money for the Dutch Cancer Society @liting-chen.bsky.social
Reposted by Dorien Pastoors
Together with more than a hundred cancer researchers, we are up for the challenge for going up to >6000m elevation with cycling, running, and walking in one day at Alpe d'HuZes event this Thursday to support research --> to outsmart cancer!
#outsmartingcancer
@oncodeinstitute.bsky.social
#outsmartingcancer
@oncodeinstitute.bsky.social
June 3, 2025 at 12:00 PM
Together with more than a hundred cancer researchers, we are up for the challenge for going up to >6000m elevation with cycling, running, and walking in one day at Alpe d'HuZes event this Thursday to support research --> to outsmart cancer!
#outsmartingcancer
@oncodeinstitute.bsky.social
#outsmartingcancer
@oncodeinstitute.bsky.social
Wow this is so cool! Congratulations !!
our work on the molecular differences between transcription factor isoforms is out now in Molecular Cell!
key point: 2/3rds of TF isos differ in properties like DNA binding & transcriptional activity
many are "negative regulators" & misexpressed in cancer
www.sciencedirect.com/science/arti...
key point: 2/3rds of TF isos differ in properties like DNA binding & transcriptional activity
many are "negative regulators" & misexpressed in cancer
www.sciencedirect.com/science/arti...
March 27, 2025 at 6:40 AM
Wow this is so cool! Congratulations !!
Really enjoyed the meeting too and was very happy to be awarded the presentation prize 🤩!! Extra festive touch added by @bloodadvances.bsky.social which published @massaarsanne.bsky.social 's article on this project DURING her talk 👏 @oncodeinstitute.bsky.social #oncode #kwf #hemesky
Enjoyed last week's KWF Dutch Cancer Biology Meeting in the forests 🌳 of Lunteren where I got the opportunity to present on DNMT3A double mutant AML.
@dpastoors.bsky.social gave nice talk on p300&CBP regulating enhancer activity in AML which was awarded with the Best Presentation Award! 🤩🥳
#Hemesky
@dpastoors.bsky.social gave nice talk on p300&CBP regulating enhancer activity in AML which was awarded with the Best Presentation Award! 🤩🥳
#Hemesky
March 21, 2025 at 10:42 AM
Really enjoyed the meeting too and was very happy to be awarded the presentation prize 🤩!! Extra festive touch added by @bloodadvances.bsky.social which published @massaarsanne.bsky.social 's article on this project DURING her talk 👏 @oncodeinstitute.bsky.social #oncode #kwf #hemesky
Wooo - check the cover image of the current issue of Blood Advances by @massaarsanne.bsky.social & Emma Boertjes et al!!! Congratulations - methylation damage repair is impaired by double mutations in DNMT3A in AML
March 21, 2025 at 7:26 AM
Wooo - check the cover image of the current issue of Blood Advances by @massaarsanne.bsky.social & Emma Boertjes et al!!! Congratulations - methylation damage repair is impaired by double mutations in DNMT3A in AML
Reposted by Dorien Pastoors
Mike spotted this before the rest of us. 🫡
Publications from 2025 are shared more on Bluesky than on X/Twitter.
Publications from 2025 are shared more on Bluesky than on X/Twitter.
About to talk to medical affairs colleagues, with the news that Bluesky is carrying more posts about new journal publications than X #bluesky #altmetrics #x #xparrot
March 13, 2025 at 3:51 PM
Mike spotted this before the rest of us. 🫡
Publications from 2025 are shared more on Bluesky than on X/Twitter.
Publications from 2025 are shared more on Bluesky than on X/Twitter.
Reposted by Dorien Pastoors
Amazing collection of reviews on developmental hematopoiesis from leaders in the field. Just out in @iseh.bsky.social journal, Experimental Hematology www.exphem.org/content/edit...
Editorial_Making_blood_Early_Hematopoietic_Development
www.exphem.org
March 12, 2025 at 8:15 PM
Amazing collection of reviews on developmental hematopoiesis from leaders in the field. Just out in @iseh.bsky.social journal, Experimental Hematology www.exphem.org/content/edit...
I seem to vaguely remember that hematopoiesis is more active during sleep. Does anyone know if this is true 😅 ? #hemesky. not necessarily hspcs, but also more mature subsets. Is most cell production in the bm at night or during the day? Is it related to sleep or day and night ?
March 5, 2025 at 4:49 PM
I seem to vaguely remember that hematopoiesis is more active during sleep. Does anyone know if this is true 😅 ? #hemesky. not necessarily hspcs, but also more mature subsets. Is most cell production in the bm at night or during the day? Is it related to sleep or day and night ?