Daniele Campisi
@danielecampisi.bsky.social
Postdoc Fellow at Institut Pasteur in Paris | #Autophagy and #trafficking - 🇮🇹 in 🇫🇷 #CellBiologist #CLEM #ElectronMicroscopy #SuperResolution and #pizza🍕
Pinned
The Role of WIPI2, ATG16L1 and ATG12-ATG5 in Selective and Nonselective Autophagy
Autophagy is a conserved cellular recycling pathway that delivers damaged or superfluous cytoplasmic material to lysosomes for degradation. In respons…
www.sciencedirect.com
Thrilled to share my latest review on the role of the #WIPI2 and #ATG16L1 complex in both selective and nonselective #autophagy 🔍🧬 It’s fascinating how these two forms of autophagy rely on the same core machinery, yet lead to such different outcomes. What defines this divergence? Have a look here 👀
Reposted by Daniele Campisi
My quote of the day
Leadership means forming a team and working toward common objectives that are tied to time, metrics, and resources.
Russel Honoré
Leadership means forming a team and working toward common objectives that are tied to time, metrics, and resources.
Russel Honoré
November 19, 2025 at 11:02 AM
My quote of the day
Leadership means forming a team and working toward common objectives that are tied to time, metrics, and resources.
Russel Honoré
Leadership means forming a team and working toward common objectives that are tied to time, metrics, and resources.
Russel Honoré
Reposted by Daniele Campisi
📈 Record interest in ERC Starting Grants 2026!
4,807 proposals - a 22% increase compared to last year’s call.
👩🔬 Women submitted nearly 43% of proposals - the highest share ever recorded in an ERC call.
More stats 👉 bit.ly/4hcYJln
#ChooseEurope
4,807 proposals - a 22% increase compared to last year’s call.
👩🔬 Women submitted nearly 43% of proposals - the highest share ever recorded in an ERC call.
More stats 👉 bit.ly/4hcYJln
#ChooseEurope
Starting Grants 2026 applications: Facts and figures
The ERC Starting Grants 2026 call closed for applications on 14 October 2025. These are the preliminary data on submitted proposals:
bit.ly
October 16, 2025 at 12:40 PM
📈 Record interest in ERC Starting Grants 2026!
4,807 proposals - a 22% increase compared to last year’s call.
👩🔬 Women submitted nearly 43% of proposals - the highest share ever recorded in an ERC call.
More stats 👉 bit.ly/4hcYJln
#ChooseEurope
4,807 proposals - a 22% increase compared to last year’s call.
👩🔬 Women submitted nearly 43% of proposals - the highest share ever recorded in an ERC call.
More stats 👉 bit.ly/4hcYJln
#ChooseEurope
Reposted by Daniele Campisi
Excited to share our #BioSenSRRF preprint on bioRxiv! 🎉
We show how SRRF microscopy can be used with FRET-based biosensors to 🔬mitochondrial functions with high resolution 😍.
Code and test data are open (GitHub + Zenodo).
👏 to Nicolas, Pierre-Jean, and co-authors! www.biorxiv.org/content/10.1...
We show how SRRF microscopy can be used with FRET-based biosensors to 🔬mitochondrial functions with high resolution 😍.
Code and test data are open (GitHub + Zenodo).
👏 to Nicolas, Pierre-Jean, and co-authors! www.biorxiv.org/content/10.1...
Combining FRET and super-resolution microscopy reveals kinase activation and mitochondrial activity at the nanoscale
Protein kinases are key regulators of intracellular signaling, showing activities in specific subcellular compartments and in micro- or nano-domains. Genetically encoded biosensors based on Förster′s ...
www.biorxiv.org
October 13, 2025 at 11:37 AM
Excited to share our #BioSenSRRF preprint on bioRxiv! 🎉
We show how SRRF microscopy can be used with FRET-based biosensors to 🔬mitochondrial functions with high resolution 😍.
Code and test data are open (GitHub + Zenodo).
👏 to Nicolas, Pierre-Jean, and co-authors! www.biorxiv.org/content/10.1...
We show how SRRF microscopy can be used with FRET-based biosensors to 🔬mitochondrial functions with high resolution 😍.
Code and test data are open (GitHub + Zenodo).
👏 to Nicolas, Pierre-Jean, and co-authors! www.biorxiv.org/content/10.1...
Reposted by Daniele Campisi
Hi all. This is the recording of the online seminar I gave following the kind invitation of #GLOBIAS.
I share what we have learned delivering #BioimageAnalysis services in the IAH core facility of the @institutpasteur.bsky.social
research.pasteur.fr/en/team/imag...
⬇️
I share what we have learned delivering #BioimageAnalysis services in the IAH core facility of the @institutpasteur.bsky.social
research.pasteur.fr/en/team/imag...
⬇️
October 13, 2025 at 8:10 AM
Hi all. This is the recording of the online seminar I gave following the kind invitation of #GLOBIAS.
I share what we have learned delivering #BioimageAnalysis services in the IAH core facility of the @institutpasteur.bsky.social
research.pasteur.fr/en/team/imag...
⬇️
I share what we have learned delivering #BioimageAnalysis services in the IAH core facility of the @institutpasteur.bsky.social
research.pasteur.fr/en/team/imag...
⬇️
Reposted by Daniele Campisi
I am hiring! - looking for a Staff Scientist to co-run my research group with me. Staff Scientist is a senior professional scientist role at EMBL. Please forward to people you might know who could be interested! embl.wd103.myworkdayjobs.com/en-US/EMBL/j...
Staff Scientist
About EMBL-EBI EMBL’s European Bioinformatics Institute is a data powerhouse, utilised on a global scale to advance scientific discovery through bioinformatics and solutions to some of the world’s mos...
embl.wd103.myworkdayjobs.com
October 10, 2025 at 7:30 AM
I am hiring! - looking for a Staff Scientist to co-run my research group with me. Staff Scientist is a senior professional scientist role at EMBL. Please forward to people you might know who could be interested! embl.wd103.myworkdayjobs.com/en-US/EMBL/j...
Reposted by Daniele Campisi
Excited to share our latest manuscript by @saikat2025.bsky.social and an international team, reporting variants in the EARP- and GARP-interacting protein EIPR1 associated with a neurodevelopmental disorder. Will send you the PDF if you can’t access the article: academic.oup.com/brain/articl...
EIPR1 variants cause a neurodevelopmental disorder with endolysosomal and dense core vesicle defects
Abstract. EIPR1 (EARP-interacting protein 1, formerly known as TSSC1) is a WD40-domain protein that interacts with the EARP (endosome-associated recycling
academic.oup.com
October 8, 2025 at 3:33 PM
Excited to share our latest manuscript by @saikat2025.bsky.social and an international team, reporting variants in the EARP- and GARP-interacting protein EIPR1 associated with a neurodevelopmental disorder. Will send you the PDF if you can’t access the article: academic.oup.com/brain/articl...
Reposted by Daniele Campisi
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
September 13, 2025 at 12:05 AM
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
Reposted by Daniele Campisi
Excited to share the first paper from the lab! 🥳 The ATG9 and lipid droplets saga continues! Open source!
Liver-specific loss of Atg9a perturbs lipid metabolism and hepatocyte integrity
The autophagy-related protein ATG9A is integral to cellular autophagy and lipid mobilization, yet its importance in mammalian physiology remains underexplored. Using a liver-specific conditional At...
doi.org
September 3, 2025 at 4:56 PM
Excited to share the first paper from the lab! 🥳 The ATG9 and lipid droplets saga continues! Open source!
Reposted by Daniele Campisi
New from @depaceraffa.bsky.social from our lab #NICHD #NIH in collaboration with Adeline Vanderver @childrensphila.bsky.social and colleagues reporting mutations in the BLOC1S1 subunit of the BLOC-1 and BORC complexes in children with a neurodevelopmental disorder www.medrxiv.org/content/10.1...
August 24, 2025 at 2:37 PM
New from @depaceraffa.bsky.social from our lab #NICHD #NIH in collaboration with Adeline Vanderver @childrensphila.bsky.social and colleagues reporting mutations in the BLOC1S1 subunit of the BLOC-1 and BORC complexes in children with a neurodevelopmental disorder www.medrxiv.org/content/10.1...
Reposted by Daniele Campisi
These are the most challenging times for early-career scientists and engineers.
My team is doing its bit to help by maintaining and even expanding our database of funding opportunities for early-career researchers.
We found 437 of them.
Download it freely here: research.jhu.edu/rdt/funding-...
My team is doing its bit to help by maintaining and even expanding our database of funding opportunities for early-career researchers.
We found 437 of them.
Download it freely here: research.jhu.edu/rdt/funding-...
August 11, 2025 at 11:54 AM
These are the most challenging times for early-career scientists and engineers.
My team is doing its bit to help by maintaining and even expanding our database of funding opportunities for early-career researchers.
We found 437 of them.
Download it freely here: research.jhu.edu/rdt/funding-...
My team is doing its bit to help by maintaining and even expanding our database of funding opportunities for early-career researchers.
We found 437 of them.
Download it freely here: research.jhu.edu/rdt/funding-...
Reposted by Daniele Campisi
📆 Mark your calendars!
The 2026 Keystone Symposium on Autophagy: Mechanisms to Therapy will take place February 2–5, 2026.
🔬 Check out the featured program and make note of key deadlines for registration and abstract submissions 👇
The 2026 Keystone Symposium on Autophagy: Mechanisms to Therapy will take place February 2–5, 2026.
🔬 Check out the featured program and make note of key deadlines for registration and abstract submissions 👇
August 18, 2025 at 4:56 PM
📆 Mark your calendars!
The 2026 Keystone Symposium on Autophagy: Mechanisms to Therapy will take place February 2–5, 2026.
🔬 Check out the featured program and make note of key deadlines for registration and abstract submissions 👇
The 2026 Keystone Symposium on Autophagy: Mechanisms to Therapy will take place February 2–5, 2026.
🔬 Check out the featured program and make note of key deadlines for registration and abstract submissions 👇
Reposted by Daniele Campisi
*ATG9A alert*👀👀
Curious about how ATG9A maintains lysosomal homeostasis via PI4P? Check out the new work from my postdoc in @satooze.bsky.social lab.
Thrilled to see this finally published!
Special thanks to @ealmacellas.bsky.social
www.sciencedirect.com/science/arti...
Curious about how ATG9A maintains lysosomal homeostasis via PI4P? Check out the new work from my postdoc in @satooze.bsky.social lab.
Thrilled to see this finally published!
Special thanks to @ealmacellas.bsky.social
www.sciencedirect.com/science/arti...
ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair
Lysosome damage activates multiple pathways to prevent lysosome-dependent cell death, including a repair mechanism involving endoplasmic reticulum (ER…
www.sciencedirect.com
June 3, 2025 at 7:22 AM
*ATG9A alert*👀👀
Curious about how ATG9A maintains lysosomal homeostasis via PI4P? Check out the new work from my postdoc in @satooze.bsky.social lab.
Thrilled to see this finally published!
Special thanks to @ealmacellas.bsky.social
www.sciencedirect.com/science/arti...
Curious about how ATG9A maintains lysosomal homeostasis via PI4P? Check out the new work from my postdoc in @satooze.bsky.social lab.
Thrilled to see this finally published!
Special thanks to @ealmacellas.bsky.social
www.sciencedirect.com/science/arti...
Reposted by Daniele Campisi
We are looking for someone to join the group as a postdoc to help us with scaling implicit transfer operators. If you are interested in this, please reach out to me through email. Include CV, with publications and brief motivational statement. RTs appreciated!
May 27, 2025 at 1:23 PM
We are looking for someone to join the group as a postdoc to help us with scaling implicit transfer operators. If you are interested in this, please reach out to me through email. Include CV, with publications and brief motivational statement. RTs appreciated!
Thrilled to share my latest review on the role of the #WIPI2 and #ATG16L1 complex in both selective and nonselective #autophagy 🔍🧬 It’s fascinating how these two forms of autophagy rely on the same core machinery, yet lead to such different outcomes. What defines this divergence? Have a look here 👀
The Role of WIPI2, ATG16L1 and ATG12-ATG5 in Selective and Nonselective Autophagy
Autophagy is a conserved cellular recycling pathway that delivers damaged or superfluous cytoplasmic material to lysosomes for degradation. In respons…
www.sciencedirect.com
May 22, 2025 at 9:46 AM
Thrilled to share my latest review on the role of the #WIPI2 and #ATG16L1 complex in both selective and nonselective #autophagy 🔍🧬 It’s fascinating how these two forms of autophagy rely on the same core machinery, yet lead to such different outcomes. What defines this divergence? Have a look here 👀
Dear #autophagy -sts, I am excited to share our intriguing review 🤩 you know that the exact originis and dynamics of #Omegasomes formation remains surprisingly mysterious. There are still puzzles to solve. Dive into what we hypothesize 🥳 portlandpress.com/biochemsoctr... @biochemsoc.bsky.social
Biogenesis of omegasomes and autophagosomes in mammalian autophagy
Autophagy is a highly conserved catabolic pathway that maintains cellular homeostasis by promoting the degradation of damaged or superfluous cytoplasmic material. A hallmark of autophagy is the genera...
portlandpress.com
May 20, 2025 at 9:57 AM
Dear #autophagy -sts, I am excited to share our intriguing review 🤩 you know that the exact originis and dynamics of #Omegasomes formation remains surprisingly mysterious. There are still puzzles to solve. Dive into what we hypothesize 🥳 portlandpress.com/biochemsoctr... @biochemsoc.bsky.social
Reposted by Daniele Campisi
Chronic stress, impaired autophagy, and depression (as opposed to acute stress)
www.nature.com/articles/d41...
www.nature.com/articles/d41...
Chronic stress drives depression by disrupting cellular housekeeping
Boosting the recycling process known as autophagy — impaired in the brain during prolonged stress — has the potential to restore normal neuronal activity and treat depression.
www.nature.com
April 10, 2025 at 2:34 PM
Chronic stress, impaired autophagy, and depression (as opposed to acute stress)
www.nature.com/articles/d41...
www.nature.com/articles/d41...
April 16, 2025 at 12:27 PM
Such a chance! Great opportunity and PI mentorship.
Our lab at the NIH (Bethesda, MD) is looking for postdoctoral fellows to join our team studying the molecular mechanisms of protein trafficking and their links to neurodevelopmental disorders starting on or after 10-1-2025. Send your application to juan.bonifacino@nih.gov. Please share and repost!
April 14, 2025 at 9:17 PM
Such a chance! Great opportunity and PI mentorship.
For all #autophagy lovers, could anyone suggest a selective inhibitor of #ULK1/2 for use on cells in culture?
Any Experience with MR-2088?
Thank you 🙏
Any Experience with MR-2088?
Thank you 🙏
January 20, 2025 at 11:32 AM
For all #autophagy lovers, could anyone suggest a selective inhibitor of #ULK1/2 for use on cells in culture?
Any Experience with MR-2088?
Thank you 🙏
Any Experience with MR-2088?
Thank you 🙏
Reposted by Daniele Campisi
Love this.. 👏
MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony
www.biorxiv.org/content/10.1...
MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony
www.biorxiv.org/content/10.1...
January 15, 2025 at 4:23 PM
Love this.. 👏
MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony
www.biorxiv.org/content/10.1...
MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony
www.biorxiv.org/content/10.1...
A perfect way to end this wonderful experience at #CellBio24 ! Pietro De Camilli @PDClab presenting lipid transport proteins in different physiological contexts! Thanks to the organizers for this inspiring week!
December 18, 2024 at 8:14 PM
A perfect way to end this wonderful experience at #CellBio24 ! Pietro De Camilli @PDClab presenting lipid transport proteins in different physiological contexts! Thanks to the organizers for this inspiring week!
Greetings from San Diego and reconnecting with nice friends! @Asmita_asmi_ @p_norell !!! #CellBio24
December 16, 2024 at 1:51 AM
Greetings from San Diego and reconnecting with nice friends! @Asmita_asmi_ @p_norell !!! #CellBio24
The first day at #CellBio24 is gone! A day full of excellent talks: “Organizing Your Lab and Your Lab Budget” for future junior PIs. Next, “membrane trafficking” and “lipids in organelle form and function” to end with a superb keynote given by Lorenz Studer. Can’t wait for day number 2! 😍
December 15, 2024 at 3:31 AM
The first day at #CellBio24 is gone! A day full of excellent talks: “Organizing Your Lab and Your Lab Budget” for future junior PIs. Next, “membrane trafficking” and “lipids in organelle form and function” to end with a superb keynote given by Lorenz Studer. Can’t wait for day number 2! 😍
Are we ready for tomorrow? 😍 #CellBio2024 #SanDiego
December 14, 2024 at 6:11 AM
Are we ready for tomorrow? 😍 #CellBio2024 #SanDiego
This is impressive 🫠🔥
ÅNGSTRÖM-RESOLUTION FLUORESCENCE MICROSCOPY.
Can we do structural biology with an optical microscope? Yes!
Check out how we resolved *single bases of DNA* and much more using fluorescence microscopy, out on 24.05.2023 in Nature! @natureportfolio.bsky.social
[re-post from Twitter]
Can we do structural biology with an optical microscope? Yes!
Check out how we resolved *single bases of DNA* and much more using fluorescence microscopy, out on 24.05.2023 in Nature! @natureportfolio.bsky.social
[re-post from Twitter]
Ångström-resolution fluorescence microscopy - Nature
The authors introduce a single-molecule DNA-barcoding method, resolution enhancement by sequential imaging, that improves the resolution of fluorescence microscopy down to the Ångström scale using off...
www.nature.com
November 27, 2024 at 6:41 PM
This is impressive 🫠🔥