Selenocysteine, incorporated into polypeptides at recoded termination codons, plays an essential role in redox biology. Using GPX1 and GPX4, selenoenzymes that mitigate oxidative stress, as reporters,...

Evolution has shaped the genetic code, with subtle pressures leading to preferences for some synonymous codons over others. Codons are translated at different speeds by the ribosome, imposing constrai...

Nanopore direct RNA sequencing (DRS) holds promise for advancing our understanding of the epitranscriptome by detecting RNA modifications in native RNA molecules. Recently, Oxford Nanopore Technologie...

Inside our cells, ribosomes—the tireless "protein factories" of life—have just shown off a new skill they haven't used in billions of years.

Exclusive: Changes to workers’ rights bill will prohibit the silencing of staff who suffer harassment or discrimination * Labour has delivered where Tory ministers did not on NDA rules Bosses in the UK will be banned from using non-disclosure agreements to silence employees who have suffered harassment and discrimination in the workplace as part of the government’s overhaul of workers’ rights. Ministers will on Monday night table amendments to the government’s employment rights bill to prohibit the widespread practice of using legally enforceable NDAs to conceal unacceptable behaviour at work. Continue reading...

A web app for drawing and exploring nucleic acid structures.

Nanopore direct RNA sequencing (dRNA-seq) enables unique insights into RNA biology. However, applications are currently limited by the lack of accurate and cost-effective sample multiplexing. Here we introduce WarpDemuX, an ultra-fast and highly accurate adapter-barcoding and demultiplexing approach for dRNA-seq with SQK-RNA002 and SQK-RNA004 chemistries. WarpDemuX enhances speed and accuracy by fast processing of the raw nanopore signal, use of a light-weight machine-learning algorithm and design of optimized barcode sets. We demonstrate its utility by performing rapid phenotypic profiling of different SARS-CoV-2 viruses through multiplexed sequencing of longitudinal samples on a single flowcell, identifying systematic differences in transcript abundance and poly(A) tail lengths during infection. Additionally, integrating WarpDemuX into sequencing control software enables real-time enrichment of target molecules through barcode-specific adaptive sampling, which we demonstrate by enric

Newborns will have whole genome sequencing to enable personalised healthcare that predicts and prevents illness

Translation termination is essential in all living organisms because it ensures that proteins have lengths strictly defined by their genes. This universal process is mediated by peptide release factor...

Defining viral proteomes is crucial to understanding viral life cycles and immune recognition but the landscape of translated regions remains unknown for most viruses. We have developed massively para...

CircRNAs are generated during splicing, but their specific degradation pathways are poorly understood. Latini et al. identify and biochemically characterize the exo- and endoribonuclease DIS3 as a candidate for cytoplasmic circRNA degradation. DIS3 uses its catalytic PIN domain to cleave circRNAs and functions in the cytoplasm independently of the exosome.