Karl Carlström
@carlstrom.bsky.social
Assistant Professor @ki.se
ki.se/en/research/team-karl-carlstrom
• epigenetics • metabolism • genome engineering • oligodendrocytes • Multiple sclerosis •
ki.se/en/research/team-karl-carlstrom
• epigenetics • metabolism • genome engineering • oligodendrocytes • Multiple sclerosis •
Pinned
Karl Carlström
@carlstrom.bsky.social
· Nov 13
www.biorxiv.org
New preprint from
@gocastelobranco.bsky.social lab!! We use MPRA & scCRISPRi/a to interrogate possible functions of GWAS-identified Multiple sclerosis risk SNPs in iPSC-derived oligodendrocytes.
www.biorxiv.org/content/10.1...
@gocastelobranco.bsky.social lab!! We use MPRA & scCRISPRi/a to interrogate possible functions of GWAS-identified Multiple sclerosis risk SNPs in iPSC-derived oligodendrocytes.
www.biorxiv.org/content/10.1...
Reposted by Karl Carlström
CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis: @cellpress.bsky.social www.cell.com/cell/fulltex...
January 28, 2026 at 3:52 PM
CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis: @cellpress.bsky.social www.cell.com/cell/fulltex...
Reposted by Karl Carlström
3 papers in Cell @cellpress.bsky.social today exploring the mechanistic connection between Epstein-Barr virus and multiple sclerosis #MultipleSclerosis
cell.com/cell/fulltex...
cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
cell.com/cell/fulltex...
cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
January 13, 2026 at 4:25 PM
3 papers in Cell @cellpress.bsky.social today exploring the mechanistic connection between Epstein-Barr virus and multiple sclerosis #MultipleSclerosis
cell.com/cell/fulltex...
cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
cell.com/cell/fulltex...
cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
Reposted by Karl Carlström
Microglia checkpoints promote healthy myelin aging
Our new paper is out in Nature Neuroscience @natneuro.nature.com
www.nature.com/articles/s41...
🧵1/3
Our new paper is out in Nature Neuroscience @natneuro.nature.com
www.nature.com/articles/s41...
🧵1/3
TGFβ signaling mediates microglial resilience to spatiotemporally restricted myelin degeneration - Nature Neuroscience
Zhu et al. find that aging causes region-specific myelin damage in the spinal cord, which is counteracted by enhanced TGFβ signaling in microglia, revealing a protective mechanism for healthy aging.
www.nature.com
January 2, 2026 at 10:16 AM
Microglia checkpoints promote healthy myelin aging
Our new paper is out in Nature Neuroscience @natneuro.nature.com
www.nature.com/articles/s41...
🧵1/3
Our new paper is out in Nature Neuroscience @natneuro.nature.com
www.nature.com/articles/s41...
🧵1/3
Reposted by Karl Carlström
With all the excitement about AI giving us answers, we often forget that the real trick is to figure out what is the question.
December 29, 2025 at 5:02 PM
With all the excitement about AI giving us answers, we often forget that the real trick is to figure out what is the question.
Reposted by Karl Carlström
There’s an art to writing a grant proposal so that your creativity is on full display. Maria Leptin - president of the ERC - talks about it on the new episode of the Night Science Podcast.
December 9, 2025 at 4:36 AM
There’s an art to writing a grant proposal so that your creativity is on full display. Maria Leptin - president of the ERC - talks about it on the new episode of the Night Science Podcast.
Reposted by Karl Carlström
First paper from the lab is now online
@natneuro.nature.com !
We mapped injury induced enhancers in the mouse CNS and decoded their sequence architecture. Little 🧵 rdcu.be/eSQi1
@natneuro.nature.com !
We mapped injury induced enhancers in the mouse CNS and decoded their sequence architecture. Little 🧵 rdcu.be/eSQi1
December 2, 2025 at 9:21 PM
First paper from the lab is now online
@natneuro.nature.com !
We mapped injury induced enhancers in the mouse CNS and decoded their sequence architecture. Little 🧵 rdcu.be/eSQi1
@natneuro.nature.com !
We mapped injury induced enhancers in the mouse CNS and decoded their sequence architecture. Little 🧵 rdcu.be/eSQi1
Reposted by Karl Carlström
Major new direction in the lab: hacking human cell biology with pathogen effectors (eORFs) - amazing collaboration with @miketilapia.bsky.social lab. Huge congrats to first authors Tomas & He & all co-authors! Check out the pre-print on @biorxivpreprint.bsky.social www.biorxiv.org/content/10.1...
www.biorxiv.org
November 18, 2025 at 2:51 PM
Major new direction in the lab: hacking human cell biology with pathogen effectors (eORFs) - amazing collaboration with @miketilapia.bsky.social lab. Huge congrats to first authors Tomas & He & all co-authors! Check out the pre-print on @biorxivpreprint.bsky.social www.biorxiv.org/content/10.1...
Reposted by Karl Carlström
New 🧬✂️ pre-print! We show that paired prime editing can efficiently generate large deletions — even >1 Mb — with high precision and at scale. We use this to perform the first pooled prime deletion screen across the human genome.
🔗 biorxiv.org/content/10.1...
A short thread (by Juliane Weller)👇
🔗 biorxiv.org/content/10.1...
A short thread (by Juliane Weller)👇
Generating long deletions across the genome with pooled paired prime editing screens
Engineered deletions are a powerful probe for studying genome architecture, function, and regulation. Yet, the lack of effective methods to create them in large numbers and at multi-kilobase scale has...
biorxiv.org
November 5, 2025 at 2:17 PM
New 🧬✂️ pre-print! We show that paired prime editing can efficiently generate large deletions — even >1 Mb — with high precision and at scale. We use this to perform the first pooled prime deletion screen across the human genome.
🔗 biorxiv.org/content/10.1...
A short thread (by Juliane Weller)👇
🔗 biorxiv.org/content/10.1...
A short thread (by Juliane Weller)👇
We are hiring!!
Interested in applying various CRISPR editing approaches and cell profiling to address functional metabolic and epigenetic interplay in oligodendrocytes?!
Apply here 👇
www.linkedin.com/posts/karl-c...
Interested in applying various CRISPR editing approaches and cell profiling to address functional metabolic and epigenetic interplay in oligodendrocytes?!
Apply here 👇
www.linkedin.com/posts/karl-c...
We are looking for a Research Assistant with an interest in functional metabolic and epigenetic interplay in oligodendrocytes!
To study this we apply a wide range of leading-edge technologies… | Kar...
We are looking for a Research Assistant with an interest in functional metabolic and epigenetic interplay in oligodendrocytes!
To study this we apply a wide range of leading-edge technologies, inclu...
www.linkedin.com
November 27, 2025 at 4:27 PM
We are hiring!!
Interested in applying various CRISPR editing approaches and cell profiling to address functional metabolic and epigenetic interplay in oligodendrocytes?!
Apply here 👇
www.linkedin.com/posts/karl-c...
Interested in applying various CRISPR editing approaches and cell profiling to address functional metabolic and epigenetic interplay in oligodendrocytes?!
Apply here 👇
www.linkedin.com/posts/karl-c...
Reposted by Karl Carlström
A new study in @natneuro.nature.com by @gocastelobranco.bsky.social et al explores how oligodendrocytes respond in #MultipleSclerosis. The researchers identified distinct and early disease-related states and gene activity patterns that may help explain how #MS progresses 🧪 news.ki.se/oligodendroc...
Oligodendrocytes show early changes in multiple sclerosis
A new study from Karolinska Institutet, published in Nature Neuroscience, explores how oligodendrocytes, the cells that produce myelin in the brain and spinal cord, respond during the development of m...
news.ki.se
November 17, 2025 at 1:22 PM
A new study in @natneuro.nature.com by @gocastelobranco.bsky.social et al explores how oligodendrocytes respond in #MultipleSclerosis. The researchers identified distinct and early disease-related states and gene activity patterns that may help explain how #MS progresses 🧪 news.ki.se/oligodendroc...
Reposted by Karl Carlström
Our paper, driven by Chao Zheng and Bastien Hervé in the lab @ki.se, is now out in @natneuro.nature.com!
Check it out at:
www.nature.com/articles/s41...
with @pkukanja.bsky.social, @deckardw.bsky.social, among others
Check it out at:
www.nature.com/articles/s41...
with @pkukanja.bsky.social, @deckardw.bsky.social, among others
Distinct transcriptomic and epigenomic responses of mature oligodendrocytes during disease progression in a mouse model of multiple sclerosis - Nature Neuroscience
Single-cell ATAC/RNA multiomic profiling was used to investigate how oligodendrocyte lineage cells respond across multiple disease stages within a mouse MS model, revealing early activation of immune ...
www.nature.com
November 17, 2025 at 10:30 AM
Our paper, driven by Chao Zheng and Bastien Hervé in the lab @ki.se, is now out in @natneuro.nature.com!
Check it out at:
www.nature.com/articles/s41...
with @pkukanja.bsky.social, @deckardw.bsky.social, among others
Check it out at:
www.nature.com/articles/s41...
with @pkukanja.bsky.social, @deckardw.bsky.social, among others
New preprint from
@gocastelobranco.bsky.social lab!! We use MPRA & scCRISPRi/a to interrogate possible functions of GWAS-identified Multiple sclerosis risk SNPs in iPSC-derived oligodendrocytes.
www.biorxiv.org/content/10.1...
@gocastelobranco.bsky.social lab!! We use MPRA & scCRISPRi/a to interrogate possible functions of GWAS-identified Multiple sclerosis risk SNPs in iPSC-derived oligodendrocytes.
www.biorxiv.org/content/10.1...
www.biorxiv.org
November 13, 2025 at 8:35 AM
New preprint from
@gocastelobranco.bsky.social lab!! We use MPRA & scCRISPRi/a to interrogate possible functions of GWAS-identified Multiple sclerosis risk SNPs in iPSC-derived oligodendrocytes.
www.biorxiv.org/content/10.1...
@gocastelobranco.bsky.social lab!! We use MPRA & scCRISPRi/a to interrogate possible functions of GWAS-identified Multiple sclerosis risk SNPs in iPSC-derived oligodendrocytes.
www.biorxiv.org/content/10.1...
Reposted by Karl Carlström
Chromatin fatigue: DNA repair alters the chromatin environment and introduces heritable variation in gene expression in a larger region around the lesion! Amazing achievement by @sbantele.bsky.social and Jiri Lukas published in @science.org 🙌 Happy we could contribute. See 👇
𝗖𝗮𝗻 𝗰𝗲𝗹𝗹𝘀 𝗲𝘃𝗲𝗿 𝘁𝗿𝘂𝗹𝘆 𝗿𝗲𝗰𝗼𝘃𝗲𝗿 𝗳𝗿𝗼𝗺 𝗗𝗡𝗔 𝗱𝗮𝗺𝗮𝗴𝗲?Excited to share our new study “Repair of DNA double-strand breaks leaves heritable impairment to genome function”, revealing DNA repair’s hidden cost, out now @science.org tinyurl.com/5n6zw3ye. Led by @sbantele.bsky.social and Jiri Lukas.🧵👇1/n
Repair of DNA double-strand breaks leaves heritable impairment to genome function
Upon DNA breakage, a genomic locus undergoes alterations in three-dimensional chromatin architecture to facilitate signaling and repair. Although cells possess mechanisms to repair damaged DNA, it is ...
tinyurl.com
November 12, 2025 at 3:55 AM
Chromatin fatigue: DNA repair alters the chromatin environment and introduces heritable variation in gene expression in a larger region around the lesion! Amazing achievement by @sbantele.bsky.social and Jiri Lukas published in @science.org 🙌 Happy we could contribute. See 👇
Reposted by Karl Carlström
Researchers at KI and @yale.edu have created a multidimensional, molecular map of how the mouse #brain develops after birth and how it reacts to #inflammation. The study is published in @nature.com. 🧪 #neuroscience news.ki.se/scientists-m...
November 6, 2025 at 10:45 AM
Researchers at KI and @yale.edu have created a multidimensional, molecular map of how the mouse #brain develops after birth and how it reacts to #inflammation. The study is published in @nature.com. 🧪 #neuroscience news.ki.se/scientists-m...
Reposted by Karl Carlström
Researchers @ki.se and Yale University have created a multidimensional, molecular map of how the mouse #brain develops after birth and how it reacts to #inflammation #research supported by Knut and Alice Wallenberg Foundation #MS #neuroscience
@gocastelobranco.bsky.social
news.ki.se/scientists-m...
@gocastelobranco.bsky.social
news.ki.se/scientists-m...
Scientists map how the brain develops – and how it resolves inflammation
Researchers at Karolinska Institutet and Yale University have created a multidimensional, molecular map of how the mouse brain develops after birth and how it reacts to inflammation. The study, which ...
news.ki.se
November 6, 2025 at 9:38 AM
Researchers @ki.se and Yale University have created a multidimensional, molecular map of how the mouse #brain develops after birth and how it reacts to #inflammation #research supported by Knut and Alice Wallenberg Foundation #MS #neuroscience
@gocastelobranco.bsky.social
news.ki.se/scientists-m...
@gocastelobranco.bsky.social
news.ki.se/scientists-m...
Latest from the lab in colab with @rongfan8.bsky.social
Our latest collaboration with @rongfan8.bsky.social, spatial triomics to investigate myelin and cortical brain development, and demyelination, spearheaded by Leslie Kirby in our lab @ki.se and Di Zhang in Rong's lab! Check it out @nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Spatial dynamics of brain development and neuroinflammation - Nature
A tri-omic atlas of the mouse brain from postnatal day 0 to P21 reveals that layer-specific projection neurons have a role in coordinating axonogenesis and myelination.
www.nature.com
November 5, 2025 at 4:16 PM
Latest from the lab in colab with @rongfan8.bsky.social
Reposted by Karl Carlström
Låter som något äkta gnällisar skulle säga.
October 20, 2025 at 1:58 PM
Låter som något äkta gnällisar skulle säga.
Reposted by Karl Carlström
🧠 The Lipid #Brain Atlas is out now! If you think #lipids are boring and membranes are all the same, prepare to be surprised. Led by @lucafusarbassini.bsky.social with Giovanni D'Angelo's lab, we mapped membrane lipids in the mouse brain at high resolution.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
October 16, 2025 at 6:23 AM
🧠 The Lipid #Brain Atlas is out now! If you think #lipids are boring and membranes are all the same, prepare to be surprised. Led by @lucafusarbassini.bsky.social with Giovanni D'Angelo's lab, we mapped membrane lipids in the mouse brain at high resolution.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
Reposted by Karl Carlström
Our study on neuroblastoma developmental plasticity is the cover story in the latest issue of Dev. Cell @cp-devcell.bsky.social .The Chinese-ink Taiji (Yin–Yang) cover to echo cell-state transitions. Huge thanks to the editors, co-authors. #Neuroblastoma www.cell.com/developmenta...
September 10, 2025 at 2:25 PM
Our study on neuroblastoma developmental plasticity is the cover story in the latest issue of Dev. Cell @cp-devcell.bsky.social .The Chinese-ink Taiji (Yin–Yang) cover to echo cell-state transitions. Huge thanks to the editors, co-authors. #Neuroblastoma www.cell.com/developmenta...
Reposted by Karl Carlström
A few weeks ago, I had to discuss about DynaTag, a smart tweak to CUT&Tag that enables single-cell transcription factor mapping with just a buffer change.
Opening a number of future avenues.
📰 Via
@GenomeWeb
: lnkd.in/djYm_sGg
#epigenetics #singlecell
Opening a number of future avenues.
📰 Via
@GenomeWeb
: lnkd.in/djYm_sGg
#epigenetics #singlecell
LinkedIn
This link will take you to a page that’s not on LinkedIn
lnkd.in
September 10, 2025 at 4:37 PM
A few weeks ago, I had to discuss about DynaTag, a smart tweak to CUT&Tag that enables single-cell transcription factor mapping with just a buffer change.
Opening a number of future avenues.
📰 Via
@GenomeWeb
: lnkd.in/djYm_sGg
#epigenetics #singlecell
Opening a number of future avenues.
📰 Via
@GenomeWeb
: lnkd.in/djYm_sGg
#epigenetics #singlecell
Reposted by Karl Carlström
Min vän Jovan har startat cykelverkstad i Gubbängens centrum cykelnomad.com. Blev en räddad palm som inflyttningspresent. Vi jobbade tillsammans för 10 år sedan och han har jobbat heltid som cykelmekaniker sen dess. Sprid gärna ordet så han kommer igång ordentligt!
September 6, 2025 at 12:48 PM
Min vän Jovan har startat cykelverkstad i Gubbängens centrum cykelnomad.com. Blev en räddad palm som inflyttningspresent. Vi jobbade tillsammans för 10 år sedan och han har jobbat heltid som cykelmekaniker sen dess. Sprid gärna ordet så han kommer igång ordentligt!
Reposted by Karl Carlström
Of David's countless accomplishments is the important work that Matt Porteus did in his lab on early-stage genome editing with engineered nucleases (pubmed 12730593).
He and Matt both speak about this in the documentary "Human Nature."
A true titan.
www.nytimes.com/2025/09/07/s...
He and Matt both speak about this in the documentary "Human Nature."
A true titan.
www.nytimes.com/2025/09/07/s...
David Baltimore, Nobel-Winning Molecular Biologist, Dies at 87
www.nytimes.com
September 7, 2025 at 9:35 PM
Of David's countless accomplishments is the important work that Matt Porteus did in his lab on early-stage genome editing with engineered nucleases (pubmed 12730593).
He and Matt both speak about this in the documentary "Human Nature."
A true titan.
www.nytimes.com/2025/09/07/s...
He and Matt both speak about this in the documentary "Human Nature."
A true titan.
www.nytimes.com/2025/09/07/s...
Reposted by Karl Carlström
Doomscrolling pause to chat CRISPR libraries! Preprint describes our new, data-driven approach to combine on-target and off-target predictions much more intelligently for *selecting* guides, which we use to develop our newest Cas9 knockout library, Jacquere. Thread: www.biorxiv.org/content/10.1...
Balancing off-target and on-target considerations for optimized Cas9 CRISPR knockout library design
The continued development of high-dimensional CRISPR screen readouts, such as single-cell RNA sequencing and high-content imaging, necessitates compact libraries to enable functional interrogation at ...
www.biorxiv.org
September 7, 2025 at 5:02 PM
Doomscrolling pause to chat CRISPR libraries! Preprint describes our new, data-driven approach to combine on-target and off-target predictions much more intelligently for *selecting* guides, which we use to develop our newest Cas9 knockout library, Jacquere. Thread: www.biorxiv.org/content/10.1...
Reposted by Karl Carlström
Our new paper on metformin, myelin, mitochondria and metabolism is now out at rdcu.be/eDbFD
Thanks to Nina and all of the team!
Thanks to Nina and all of the team!
Metformin alters mitochondria-related metabolism and enhances human oligodendrocyte function
Nature Communications - Metformin is under clinical trial for brain neuroprotection but how it may work is poorly understood. Here, the authors show that metformin helps myelin formation by human...
rdcu.be
August 31, 2025 at 1:03 PM
Our new paper on metformin, myelin, mitochondria and metabolism is now out at rdcu.be/eDbFD
Thanks to Nina and all of the team!
Thanks to Nina and all of the team!
Reposted by Karl Carlström
A careful, comprehensive and creative characterization of T cell dynamics in response to pathogens, with all credits going to @rohamparsa.bsky.social who just opened his lab!
Excited to share our new preprint! We studied how T cells are functionally & clonally shaped across organs over time. With the new TRACK mice, we genetically label recently activated T cells with tdTomato and track their migration across tissues. #immunology #science www.biorxiv.org/content/10.1...
August 29, 2025 at 12:18 PM
A careful, comprehensive and creative characterization of T cell dynamics in response to pathogens, with all credits going to @rohamparsa.bsky.social who just opened his lab!