@brookscrickard1.bsky.social
Reposted
Interested in analyzing kinase activity with subcellular spatial resolution? Want to learn more about peptide barcoding?
@smolka-lab.bsky.social and @wjcomstock.bsky.social have something just for you!
@smolka-lab.bsky.social and @wjcomstock.bsky.social have something just for you!
Check out our new biosensor technology to study DDR kinase signaling: ProKAS.
We combine:
-proteomics
-engineered peptide sensors
-a new concept of amino acid barcodes
ProKAS tracks kinase signaling with spatial resolution and produces highly quantitative data.
Just published today: rdcu.be/ePNo0
We combine:
-proteomics
-engineered peptide sensors
-a new concept of amino acid barcodes
ProKAS tracks kinase signaling with spatial resolution and produces highly quantitative data.
Just published today: rdcu.be/ePNo0
November 13, 2025 at 6:53 PM
Interested in analyzing kinase activity with subcellular spatial resolution? Want to learn more about peptide barcoding?
@smolka-lab.bsky.social and @wjcomstock.bsky.social have something just for you!
@smolka-lab.bsky.social and @wjcomstock.bsky.social have something just for you!
Reposted
Delighted to see our study finally out in "print". Thanks to all the (many) contributors, collaborators and reviewers!
Mismatch repair disturbs meiotic crossover control in S. cerevisiae academic.oup.com/nar/article/...
Mismatch repair disturbs meiotic crossover control in S. cerevisiae academic.oup.com/nar/article/...
Mismatch repair disturbs meiotic crossover control in S. cerevisiae
Abstract. Crossover formation during meiosis generates genetic diversity. In many species most crossovers display interference, meaning they are spaced mor
academic.oup.com
November 13, 2025 at 8:40 PM
Delighted to see our study finally out in "print". Thanks to all the (many) contributors, collaborators and reviewers!
Mismatch repair disturbs meiotic crossover control in S. cerevisiae academic.oup.com/nar/article/...
Mismatch repair disturbs meiotic crossover control in S. cerevisiae academic.oup.com/nar/article/...
Reposted
Happy to share our @narjournal.bsky.social paper on the mechanism of Dmc1 filament stabilization by Mei5-Sae3!
We show that Mei5-Sae3 can stabilize both active and inactive Dmc1 filaments, and that this is independent of the ATP hydrolytic cycle.
doi.org/10.1093/nar/...
We show that Mei5-Sae3 can stabilize both active and inactive Dmc1 filaments, and that this is independent of the ATP hydrolytic cycle.
doi.org/10.1093/nar/...
Mei5–Sae3 stabilizes both active and inactive forms of Dmc1 filaments independently of its impact on ATP hydrolysis
Abstract. In budding yeast, Dmc1’s recombinogenic activity is controlled by the meiosis-specific heterodimer Mei5–Sae3. Mei5–Sae3 is required for assembly
doi.org
November 9, 2025 at 2:19 PM
Happy to share our @narjournal.bsky.social paper on the mechanism of Dmc1 filament stabilization by Mei5-Sae3!
We show that Mei5-Sae3 can stabilize both active and inactive Dmc1 filaments, and that this is independent of the ATP hydrolytic cycle.
doi.org/10.1093/nar/...
We show that Mei5-Sae3 can stabilize both active and inactive Dmc1 filaments, and that this is independent of the ATP hydrolytic cycle.
doi.org/10.1093/nar/...
Be careful the gates of hell are temporarily open
November 4, 2025 at 11:47 AM
Be careful the gates of hell are temporarily open
Reposted
Members of @acsbiol.bsky.social, the Fall 2025 Election is occurring now & will run until 11/28. To ensure you receive your ballot, be sure that your ACS membership is up to date & your email filters are set to receive from elections@vote-now.com.
October 31, 2025 at 8:20 PM
Members of @acsbiol.bsky.social, the Fall 2025 Election is occurring now & will run until 11/28. To ensure you receive your ballot, be sure that your ACS membership is up to date & your email filters are set to receive from elections@vote-now.com.
in the crucible
October 30, 2025 at 1:33 PM
in the crucible
I'm happy to post this recent work from our lab. Trying to keep things going under challenging times.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
Rad54 separation of function mutation highlights unique roles during homologous recombination
Homologous recombination (HR) is a DNA repair pathway that utilizes a template-based approach to repair double-strand breaks within the genome. Template utilization requires the exchange of individual...
www.biorxiv.org
October 15, 2025 at 10:48 PM
I'm happy to post this recent work from our lab. Trying to keep things going under challenging times.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
remember when data not shown and personal communication would appear at least once or twice in a paper, and error bars were optional...
October 15, 2025 at 6:26 PM
remember when data not shown and personal communication would appear at least once or twice in a paper, and error bars were optional...
What is the over/under they end up in the wrong Portland...
September 27, 2025 at 5:06 PM
What is the over/under they end up in the wrong Portland...
Reposted
WE'RE HIRING: Cornell University’s Weill Institute for Cell & Molecular Biology and the Department of Molecular Biology & Genetics are seeking a tenure-track Assistant Professor whose research integrates cell and molecular biology with an evolutionary perspective.
apps.hr.cornell.edu/recruiting/f...
apps.hr.cornell.edu/recruiting/f...
Academic Career Opportunities
apps.hr.cornell.edu
September 22, 2025 at 3:55 PM
WE'RE HIRING: Cornell University’s Weill Institute for Cell & Molecular Biology and the Department of Molecular Biology & Genetics are seeking a tenure-track Assistant Professor whose research integrates cell and molecular biology with an evolutionary perspective.
apps.hr.cornell.edu/recruiting/f...
apps.hr.cornell.edu/recruiting/f...
Reposted
🌟 Congratulations to @weillinstitute.bsky.social founding director Scott Emr, on winning the prestigious World Laureate Association Prize! His landmark work on the ESCRT pathway reshapes our understanding of infection, cancer & neurodegeneration. 🌟
news.cornell.edu/stories/2025...
news.cornell.edu/stories/2025...
Emr wins the World Laureate Association Prize | Cornell Chronicle
Scott Emr, the Samuel C. and Nancy M. Fleming Professor Emeritus in the College of Arts and Sciences, has won the World Laureate Association Prize, one of the world’s highest-funded scientific awards.
news.cornell.edu
September 18, 2025 at 1:11 PM
🌟 Congratulations to @weillinstitute.bsky.social founding director Scott Emr, on winning the prestigious World Laureate Association Prize! His landmark work on the ESCRT pathway reshapes our understanding of infection, cancer & neurodegeneration. 🌟
news.cornell.edu/stories/2025...
news.cornell.edu/stories/2025...
Just throwing this out there as a significant conceptual advance in the field
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
The Eukaryotic homology search complex distorts donor DNA structure to probe for homology
Homologous recombination (HR) is a DNA double-strand break repair pathway that facilitates genetic exchange and protects damaged replication forks during DNA synthesis. As a template-based repair proc...
www.biorxiv.org
September 17, 2025 at 6:23 PM
Just throwing this out there as a significant conceptual advance in the field
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted
I couldn’t be more proud of this tremendous work by Dr @awood2.bsky.social in my lab, co-led by myself and @idwolff.bsky.social. Anna worked tirelessly to conduct a comprehensive analysis of female meiosis, showing that CNTD1 plays role in crossover designation and checkpoint regulation in females.
@awood2.bsky.social, Wolff et al. @cohenlab.bsky.social @cornellrepro.bsky.social show that cyclin N-terminal domain containing 1 (CNTD1) is a critical regulator of crossover formation during prophase I of #meiosis in females. rupress.org/jcb/article/...
#Genetics #CellCycle #CellDivision
#Genetics #CellCycle #CellDivision
June 9, 2025 at 7:24 PM
I couldn’t be more proud of this tremendous work by Dr @awood2.bsky.social in my lab, co-led by myself and @idwolff.bsky.social. Anna worked tirelessly to conduct a comprehensive analysis of female meiosis, showing that CNTD1 plays role in crossover designation and checkpoint regulation in females.
Reposted
Co-organizing a one day FREE conference on Genome Instability- Discovery to Personalized Medicine 11/1/25. Please join us! Short talks available. https//noncredit.t...
September 3, 2025 at 3:36 PM
Co-organizing a one day FREE conference on Genome Instability- Discovery to Personalized Medicine 11/1/25. Please join us! Short talks available. https//noncredit.t...
Here is a link to a new pre-print that is a collaborative effort between our lab and the lab of Michelle Wang here at Cornell.
We use single-molecule techniques to investigate how the combination of Rad51 and Rad54 remodels donor DNA during the homology search
www.biorxiv.org/cgi/content/...
We use single-molecule techniques to investigate how the combination of Rad51 and Rad54 remodels donor DNA during the homology search
www.biorxiv.org/cgi/content/...
The Eukaryotic homology search complex distorts donor DNA structure to probe for homology
Homologous recombination (HR) is a DNA double-strand break repair pathway that facilitates genetic exchange and protects damaged replication forks during DNA synthesis. As a template-based repair proc...
www.biorxiv.org
August 29, 2025 at 9:53 AM
Here is a link to a new pre-print that is a collaborative effort between our lab and the lab of Michelle Wang here at Cornell.
We use single-molecule techniques to investigate how the combination of Rad51 and Rad54 remodels donor DNA during the homology search
www.biorxiv.org/cgi/content/...
We use single-molecule techniques to investigate how the combination of Rad51 and Rad54 remodels donor DNA during the homology search
www.biorxiv.org/cgi/content/...
Reposted
Here we show that >90% of Paenibacillus encode a unique Spo0B with a transmembrane domain! TM domain helps it interact with its phosphorelay partners. Congrats to @isabellalin.bsky.social who left for grad school this week and her mentor @cassidyprints.bsky.social! www.biorxiv.org/content/10.1...
Conservation of sporulation genes and a transmembrane-containing Spo0B variant in Paenibacillus
Sporulation is a strategy employed by many bacteria to survive harsh environmental conditions. The genus Paenibacillus includes spore-forming species notorious for spoiling pasteurized dairy products ...
www.biorxiv.org
August 28, 2025 at 11:27 PM
Here we show that >90% of Paenibacillus encode a unique Spo0B with a transmembrane domain! TM domain helps it interact with its phosphorelay partners. Congrats to @isabellalin.bsky.social who left for grad school this week and her mentor @cassidyprints.bsky.social! www.biorxiv.org/content/10.1...
Reposted
De novo engineered guide RNA-directed transposition with TnpB-family proteins reveals features of naturally evolved systems www.biorxiv.org/content/10.1...
De novo engineered guide RNA-directed transposition with TnpB-family proteins reveals features of naturally evolved systems
Programmable DNA integration using CRISPR-associated transposons (CASTs) offers powerful capabilities for genome engineering. The single effector Cas12k CAST examples evolved from a fixed guide TnpB n...
www.biorxiv.org
August 28, 2025 at 12:23 PM
De novo engineered guide RNA-directed transposition with TnpB-family proteins reveals features of naturally evolved systems www.biorxiv.org/content/10.1...
Things seen today on campus:
A coyote leading a slow speed chase of police
A coyote leading a slow speed chase of police
August 27, 2025 at 10:51 PM
Things seen today on campus:
A coyote leading a slow speed chase of police
A coyote leading a slow speed chase of police
Reposted
Transcription and recombination are two universal DNA-dependent processes, but how they are coordinated remains largely unknown. Here we characterized transcription-recombination priority rules in yeast. 🧵 www.embopress.org/doi/full/10....
August 27, 2025 at 12:00 PM
Transcription and recombination are two universal DNA-dependent processes, but how they are coordinated remains largely unknown. Here we characterized transcription-recombination priority rules in yeast. 🧵 www.embopress.org/doi/full/10....
We've reached wild animals roaming campus unchecked level of dystopia...
August 26, 2025 at 7:39 PM
We've reached wild animals roaming campus unchecked level of dystopia...
Reposted
New preprint from the lab: Dr. Daniela James led an effort to improve the ChIP-exo protocol for high-res protein-DNA interactions. Particularly focused on optimizing for use in mammalian cells and making the protocol compatible with newer Illumina sequencers.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Optimized ChIP-exo for mammalian cells and patterned sequencing flow cells
By combining chromatin immunoprecipitation (ChIP) with an exonuclease digestion of protein-bound DNA fragments, ChIP-exo characterizes genome-wide protein-DNA interactions at near base-pair resolution...
www.biorxiv.org
August 23, 2025 at 7:16 PM
New preprint from the lab: Dr. Daniela James led an effort to improve the ChIP-exo protocol for high-res protein-DNA interactions. Particularly focused on optimizing for use in mammalian cells and making the protocol compatible with newer Illumina sequencers.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted
I am delighted to share that I have started my lab in the Dept. of Biochemistry @uofubiochem.bsky.social at the University of Utah @utah.edu. My laboratory will study key aspects of protein synthesis and translational control in healthy and diseased states. #RNAsky sinha.biochem.utah.edu
The Sinha Lab
The Sinha lab studies key aspects of protein synthesis and translational control in healthy and diseased states. We study the multifaceted roles of ribosomes as critical sensors of cellular stress.
sinha.biochem.utah.edu
August 21, 2025 at 7:09 PM
I am delighted to share that I have started my lab in the Dept. of Biochemistry @uofubiochem.bsky.social at the University of Utah @utah.edu. My laboratory will study key aspects of protein synthesis and translational control in healthy and diseased states. #RNAsky sinha.biochem.utah.edu
Reposted
New publication from our lab! It was really fun writing this review @jcb.org with my postdoc Dr. Chris Mellor and graduate student Elisabeth Larson. We explore how folate deficiency trigger DNA damage that disrupt genome stability.
New review: Mellor, Larson, and @drmengwang.bsky.social discuss the molecular mechanisms of mammalian genomic instability arising from folate deficiency and how this impacts genome biology in health and disease. rupress.org/jcb/article/...
#CellMetabolism
#CellMetabolism
August 21, 2025 at 1:21 PM
New publication from our lab! It was really fun writing this review @jcb.org with my postdoc Dr. Chris Mellor and graduate student Elisabeth Larson. We explore how folate deficiency trigger DNA damage that disrupt genome stability.
Reposted
Latest from the lab! Analysis of everyone’s favorite regulatory mechanism in bacteria — the RF2 programmed frameshift! Likely present in the ancestor of bacteria, use of this mechanism is influenced by stop codon usage! Big congrats to @cassidyprints.bsky.social
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
Conservation and evolution of the programmed ribosomal frameshift in prfB across the bacterial domain | mBio
Translation termination is catalyzed by one of two release factors in bacteria, RF1 or RF2. It has been known for decades that RF2 levels in Escherichia coli are regulated by a programmed ribosomal frameshift within the prfB gene that encodes RF2. We ...
journals.asm.org
August 19, 2025 at 7:44 PM
Latest from the lab! Analysis of everyone’s favorite regulatory mechanism in bacteria — the RF2 programmed frameshift! Likely present in the ancestor of bacteria, use of this mechanism is influenced by stop codon usage! Big congrats to @cassidyprints.bsky.social
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...