A wireless wearable sweat rate sensor system is presented, featuring digital 3D direct-write printing on a flexible substrate with microfluidic layers for continuous, real-time monitoring. Printed en....

The human exposome features a highly expansive chemical space and substantial individual variability. Although screenings of xenobiotic compounds have revealed exposure landscapes of specific compound...

Specialized metabolites represent a prolific source of potential drug candidates. However, the process from detecting bioactivity in a crude metabolite extract to unambiguously identifying the active agent is a tedious and expensive endeavor. Speeding up this procedure is crucial, as new drugs, such as antibiotics, are urgently needed. Furthermore, the systematic functional assessment of complex metabolome samples represents a key bottleneck in nontargeted metabolomics, which once solved, holds the potential to fundamentally advance our systematic understanding of biology. To tackle this central bioanalytical challenge, we developed a compound-resolved bioactivity-based metabolomics workflow that combines nontargeted liquid chromatography tandem mass spectrometry (LC-MS/MS), high frequency fractionation on microfluidic devices and subsequent readout with luminescent bioreporter strains. Central for this workflow is a custom high-speed (∼1 Hz frequency) fractionation device that spots the mobile phase onto a microfluidic paper-analytical device (μPAD) in parallel to MS/MS data acquisition. Subsequently, the μPAD can be overlaid with a bioreporter strain, which displays cellular stress by expressing luciferase. The luminescence signal can then be correlated to MS signals through their chromatographic profiles. We evaluated five different luciferase-expressing bioreporter strains which provide information about different antibacterial modes of action, and tested the workflow with different antibiotic standards and mixtures thereof, as well as crude extracts from the known antibiotic producer Saccharopolyspora erythraea. Our results demonstrated high sensitivity (up to 1 ng/spot, depending on compound and bioreporter) and the rapid identification of multiple antimicrobial compounds out of crude extracts, highlighting the practicality and high-throughput capability of this compound-resolved bioactivity-based metabolomics approach.

This tutorial offers a step-by-step guide for analytical chemists to train machine learning models for MS-based metabolomics. It covers data preparation, feature engineering, model selection, evaluati...

Analytical ChemistryDOI: 10.1021/acs.analchem.5c01417

President Donald Trump is expected to sign a proclamation as soon as Friday that would move to extensively overhaul the H-1B visa program, requiring a $100,000 fee for applications in a bid to curb ov...

Nature is a prolific pharmacist. Aspirin, the blood thinner warfarin and metformin for diabetes all trace back to plants, discovered by serendipity before being transformed into drugs by modern chemis...

Enveda is unlocking the vast, hidden potential of life's chemistry to solve humanity's greatest challenges. Learning from life. We are proud to work with Enveda, discover more at https://enveda.com/...

This month 16 years ago I joined my first Metabolomics Society #metabolomics #conference in Edmonton, Canada. There, I made my first steps in the #metabolomics #community by presenting a poster and ta...