The CRISPR-associated protein 9 (Cas9) produced by disease-associated strains of Campylobacter jejuni contributes to full virulence, including immune evasion and bacterial survival inside eukaryotic c...

Background CRISPR-Cas9 genome editing enables precise genetic modifications by introducing targeted DNA double-strand breaks (DSBs). While Cas9-induced DSBs are known to cause unintended on-target mut...

**Abstract:** CRISPR-Cas9 gene editing technology’s clinical success is hampered by the risk of off-target effects, where the Cas9 enzyme cleaves unintended genomic sites. Current detection methods often lack sensitivity and specificity, struggling to distinguish true off-target events from background noise. This research proposes a novel framework, Multi-Modal Integrated Off-Target Prediction Engine (MIOTPE), that integrates deep sequencing data, structural predictions of Cas9-DNA interactions, and functional assays to dramatically enhance the reliability of allele-specific off-target detection.

**Abstract:** This study introduces a novel automated workflow for optimizing ex vivo CRISPR-Cas9 gene editing targeting precise insertion of functional beta-globin sequences into the beta-globin cluster within hematopoietic stem and progenitor cells (HSPCs) to treat beta-thalassemia. Our system, termed “Kinetic Precision Editing Optimizer” (KPEO), employs a multi-layered evaluation pipeline incorporating stochastic kinetic rate equation (SKRE) modeling to predict and optimize editing outcomes, significantly improving insertion efficiency and minimizing off-target effects compared to traditional trial-and-error approaches.

Comparing the genomes of snakes with distinct coloration patterns pinpointed the genetic variants that contribute to these intricate designs.

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Hvordan udvikle nye afgrøder, der lever op til de mange krav, der blev omtalt i det foregående afsnit: Afgrøder der på den ene side kan bidrage til at løse vigtige samfundsproblemer som klimaforandrin...

Abstract. Human trisomy 21, responsible for Down syndrome, is the most prevalent genetic cause of cognitive impairment and remains a key focus for prenatal

Djamshidi et al. describe a strategy that they call fast, accurate and more efficient (FAME)-CRISPR utilizing pan-histone deacetylase inhibitors and engineered virus-like particles to improve the efficiency and speed of genome-editing experiments.

Corn snakes exhibit characteristic dorsal blotches, serving as an important model organism for investigating the genetic and developmental pathways underlying skin coloration in reptiles. Using comparative genomics and CRISPR-Cas9, researchers identified the genes that give the corn snake its characteristic dorsal blotches Image credit:LANE laboratory ( As a graduate student at the Free University of Brussels nearly 20 years ago, Athanasia Tzika analyzed the genomes of several animals to better understand their diversity. During this time, the striking, distinct patterns on reptile skin caught her attention. “Out of curiosity, you start looking at , and you start seeing…interesting characters,” said Tzika, now an evolutionary developmental biologist at the University of Geneva.

Although plants share core cell division mechanisms with other eukaryotes, their unique features, such as acentrosomal spindle formation and cytokinesis via ...