#Plasmids
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rhystwhite.bsky.social
Genomics isn’t just solving #Outbreaks, it’s stopping them before they escalate

HOT take from @natrevmicro.nature.com: If you only sequence #MDR outbreaks, you’re already late. Consider susceptible strains & #Plasmids

#GenomicSurveillance #InfectionControl

🖥️🧬💻
#AcademicSky
#MicroSky
#IDSky
🧪🧫🦠
November 11, 2025 at 10:27 AM
magicmicrobe.bsky.social
So happy to share this! Bacteriocins were first discovered over 100 years ago, but what do they actually do? We look at >1000 bacteriocin plasmids and find links to virulence and antimicrobial resistance, and frequent bacteriocin sharing in Enterobacteriaceae.
www.nature.com/articles/s41...
Bacterial warfare is associated with virulence and antimicrobial resistance - Nature Communications
Bacteria employ a range of competition systems that deliver toxins to inhibit competing strains. This study shows that these systems are particularly important for the ecology of virulent and antibiot...
www.nature.com
November 5, 2025 at 7:32 AM
alleninstitute.org
Happy #FluorescenceFriday! Our #CellLineOfTheWeek is our 3-for-1 combo - fibrillarin, nucleophosmin, and upstream binding transcription factor! 🧫✨

Our cell lines and plasmids are available for just the cost of shipping to support science.

📦 @coriellinstitute.bsky.social & @addgene.bsky.social
November 7, 2025 at 10:15 PM
davvi36.bsky.social
I thoroughly enjoyed visiting the @biology.ox.ac.uk department - thanks so much for hosting me @annadewar.bsky.social !
And thanks to everyone who chatted to me about plasmids, genomes, and more.
I even manage to find a phage in the natural history museum before leaving :)
Me with the model of a bacteriophage in the museum of natural history
November 7, 2025 at 5:40 PM
biorxiv-genetic.bsky.social
Phage-Plasmids Are Rare in Bacteria, May Exhibit Pseudolysogeny and Lack Antibiotic Resistance Genes https://www.biorxiv.org/content/10.1101/2025.11.07.687105v1
November 8, 2025 at 3:33 AM
evolvedbiofilm.bsky.social
Asaf finishes on plasmids, prophages, and defense system being depleted in plant microbiota genomes

‼️ not only in genomes, but also in metagenome datasets similar trend observed

See their Genome Biology paper
genomebiology.biomedcentral.com/articles/10....

#PMS2025
Plasmids, prophages, and defense systems are depleted from plant microbiota genomes - Genome Biology
Plant-associated bacteria significantly impact plant growth and health. Understanding how bacterial genomes adapt to plants can provide insights into their growth promotion and virulence functions. He...
genomebiology.biomedcentral.com
November 6, 2025 at 8:48 AM
annadewar.bsky.social
Great to have Dr David Sünderhauf visit Oxford over the past couple of days!

A really nice talk on competition between plasmids carrying CRISPR-Cas and Toxin-antitoxin systems. Theory, experiments and bioinformatics all in one project!

@davvi36.bsky.social @biology.ox.ac.uk
November 7, 2025 at 4:22 PM
sundermannaj.bsky.social
Our new pre-print: ACCIO 🪄🦠💨

A new tool led by @nathanraabe.bsky.social detects plasmids from short, long, & hybrid assemblies w/ high accuracy

It uses a curated local plasmid database & structural similarity metrics to infer which plasmids are present

#IDSky

Preprint: doi.org/10.1101/2025...
ACCIO: An Assembly-Based Tool Enabling Plasmid Detection
Plasmids are extrachromosomal mobile genetic elements that often carry genes responsible for antimicrobial resistance. Plasmid epidemiology aims to track the evolution and spread of plasmids, but the ...
doi.org
November 3, 2025 at 4:33 PM
emmseveri.bsky.social
#microsky #phagesky #phage plasmids & other #MGE in the bee #microbiome

www.biorxiv.org/content/10.1...
Nested Connections: Local Phage and Broad Plasmid Sharing in the Honey Bee Mobilome
Honey bees rely on bacterial symbionts for their nutritional needs and for protection against invading pathogens. Genetic diversity among strains within the colony has the potential to impact symbiont...
www.biorxiv.org
November 2, 2025 at 6:52 AM
sweetsweetplasmids.bsky.social
November #promosky

👋

Hi I’m Sweet Plasmids (Simon) and I like to waffle about video games.
Here are a few of my favourites;

🧬 Bioshock
🧙 Final Fantasy
❣️ Kingdom Hearts
👑 Elden Ring
🪓 God of War
🏹 Horizon Zero Dawn
⚪️ Destiny 2
🚀 Mass Effect
⭕️ Halo

It’s nice to meet you 🤝
November 1, 2025 at 6:34 PM
tangibullah.bsky.social
"Hey lady! Want to exchange plasmids?"
October 30, 2025 at 8:10 PM
tharrek.bsky.social
Elizabeth, but she became a splicer in Rapture
And somehow got her tits out
-
#Bioshock / #ElizabethComstock / #NSFW / #BRart
a digital sepia monochrome sketch of Elizabeth from Bioshock Infinite. She's walking down a hallway and is all spliced and deformed. Her clothes are all torn and she's dirty and bloodied. Her corset is torn open and her breasts are exposed. She's holding a bloody knife in one hand and a wrench with the other. The arm holding the wrench is covered in pustules caused by the splicing, and producing electricity from plasmids. She's looking at the viewer with a blank expression on her face. a digital sepia monochrome sketch of Elizabeth from Bioshock Infinite. She's walking down a hallway and is all spliced and deformed. Her clothes are all torn and she's dirty and bloodied. Her corset is torn open and her breasts are exposed. She's wearing a bird mask which is also all dirty. She's holding a bloody knife in one hand and a wrench with the other. The arm holding the wrench is covered in pustules caused by the splicing, and producing electricity from plasmids. She's looking at the viewer with a blank expression on her face.
October 29, 2025 at 3:23 PM
ponara.bsky.social
Today's warm-up sketch was
What if Elizabeth had become a splicer in Rapture?
-
#Bioshock / #ElizabethComstock / #BRart
a digital sepia monochrome sketch of Elizabeth from Bioshock Infinite. She's walking down a hallway and is all spliced and deformed. Her clothes are all torn and she's dirty and bloodied. She's holding a bloody knife in one hand and a wrench with the other. The arm holding the wrench is covered in pustules caused by the splicing, and producing electricity from plasmids. She's looking at the viewer with a blank expression on her face. a digital sepia monochrome sketch of Elizabeth from Bioshock Infinite. She's walking down a hallway and is all spliced and deformed. Her clothes are all torn and she's dirty and bloodied. She's wearing a bird mask which is also all dirty. She's holding a bloody knife in one hand and a wrench with the other. The arm holding the wrench is covered in pustules caused by the splicing, and producing electricity from plasmids. She's looking at the viewer with a blank expression on her face.
October 28, 2025 at 6:36 PM
ponara.bsky.social
QRP your dark art 🖤
-
#Bioshock / #MarvelRivals / #Cyberpunk2077 / #Signalis / #BRart
a digital sepia monochrome sketch of Elizabeth from Bioshock Infinite. She's walking down a hallway and is all spliced and deformed. Her clothes are all torn and she's dirty and bloodied. She's wearing a bird mask which is also all dirty. She's holding a bloody knife in one hand and a wrench with the other. The arm holding the wrench is covered in pustules caused by the splicing, and producing electricity from plasmids. She's looking at the viewer with a blank expression on her face. A digital drawing of Magik from Marvel Rivals, in a "venomized" version. She's walking slouched and carrying her sword. Her mouth is wide open showing all of her teeth and her huge glowing tongue is out. She's in a dark environment with red mist and highlights around her A digital illustration of Songbird from Cyberpunk. She's naked and seen from behind, and all her back cybernetics are visible. She's in a dark environment with red lighting, and the silhouettes of chains and cables can be seen hanging from the ceiling. a digital drawing of an ADLR unit from Signalis. She's standing on a dark environment and being lit by two beams of light, one over his chest and another over his eyes. He's turning to look somewhere behind him with a neutral expression on his face
October 29, 2025 at 5:16 PM
biorxiv-microbiol.bsky.social
Plasmid dependent phage eliminate pathogenic bacteria and antibiotic resistance plasmids from the chicken gut microbiome https://www.biorxiv.org/content/10.1101/2025.10.26.684609v1
October 27, 2025 at 3:16 AM
devbiol.bsky.social
#DBfeature

Scientists have identified a provisional regulatory circuit downstream of the transcription factor Pax3/7 operating in the descending decussating neurons (ddNs) of the tunicate Ciona robusta.

By Kim, K., Piekarz, K. M., & Stolfi, A.
doi.org/10.1016/j.yd...
Upper panel: The descending decussating neurons (ddNs) of a Ciona larva at 19.5 h post-fertilization (hpf at 20 ◦C, ~ Hotta stage 29) labeled by expression of elecroporated Dmbx/Defcab >Unc-76::YFP reporter plasmid. Scale bar =10 μm. Right: comparative diagrams of ddNs and their putative vertebrate homologs, the Mauthner cells (species depicted as an example is Danio rerio). Lower panel: A summary diagram of a ddN gene regulatory network. Diagram for a ddN-specific gene regulatory network downstream of Pax3/7. Some branches are shared with different neuron types, such as ACINs, epidermal neurons, or papilla neurons (a sub-type of epidermal neurons). Dashed arrow indicates regulatory connection supported by RNAseq data, but not validated by reporter plasmids or in situ mRNA hybridization. Grey arrow indicates possible role of Lhx1/5 in regulating maintenance, but not initial activation of Dmbx and Defcab.
October 27, 2025 at 5:04 PM
plasmidsaurus.bsky.social
Sanger sequencing is holding researchers back — it struggles with GC-rich regions, ITRs, and mixed populations. Learn how to get faster, more complete results and avoid hidden backbone errors.

Tue, 28 Oct | 10AM SGT
Register: nanoporetech.swoogo.com/ont-plasmids...

Co-hosted w/ @nanoporetech.com
October 23, 2025 at 5:08 PM
asantoslopez.bsky.social
Do plasmids really move around that much? Well, maybe not always

Thrilled to have contributed to this story with two of my favourite microbiologists: @jrpenades.bsky.social & @sanmillan.bsky.social

This great work was led by Akshay Sabnis & @wfigueroac3.bsky.social

www.cell.com/cell-reports...
Non-conjugative plasmids limit their mobility to persist in nature
Sabnis et al. explain why non-conjugative plasmids move at a low rate in nature. While increased mobility can easily evolve by incorporating phage DNA into plasmids, this is disadvantageous because it...
www.cell.com
October 22, 2025 at 5:47 PM
saintzaiya.blacksky.app
Same vibes as this
Frank Fontaine @FrankFontaineReal fuck these plasmids got me liking boys 2:23 PM • 2/7/56 from Rapture 19 Views 17 Andrew Ryan what @No.1ParaciteHater -3m 1 17 .. • 企 Frank Fontaine@FrankFontaineReal -2m Stay back! Ion wanna fuck you!! I can't contr... AHHH!!! 2 Ill 11
October 22, 2025 at 6:16 PM
wfigueroac3.bsky.social
✨New paper from @jrpenades.bsky.social and @sanmillan.bsky.social labs. We found that non-conjugative plasmids 🧬 tend to have low mobility to promote functional diversity ⚔️💊 in bacterial communities🦠🦠.
Brilliant work by Akshay, @asantoslopez.bsky.social and others.

www.cell.com/cell-reports...
Non-conjugative plasmids limit their mobility to persist in nature
Sabnis et al. explain why non-conjugative plasmids move at a low rate in nature. While increased mobility can easily evolve by incorporating phage DNA into plasmids, this is disadvantageous because it...
www.cell.com
October 22, 2025 at 1:37 PM
therealpdogue.bsky.social
Happy to share the final story from my thesis, demonstrating that the common ancestor of all terrestrial fungi had zymocin-like killer plasmids, a toxin system found in some budding yeasts. Come with me on an all-too-familiar, database dumpster-diving journey (1/10)
www.biorxiv.org/content/10.1...
Zymocin-like killer plasmids were present in the common ancestor of terrestrial fungi
Some budding yeasts secrete killer toxins made by linear dsDNA plasmids located in the cytosol. The best-known example is the Kluyveromyces lactis toxin zymocin, which is encoded by a 9-kb killer plas...
www.biorxiv.org
October 21, 2025 at 7:48 PM
zebrafishrock.bsky.social
Colorful collage of abnormal larval zebrafish hearts. Credit to @dorotheeb.bsky.social. #ZebrafishZunday 🧪
multi-color labeled zebrafish heart with mosaic expression of diverse myl7:color plasmids. Abnormal atrium due to wnt8a overexpression.
October 19, 2025 at 1:24 PM
alleninstitute.org
Happy #FluorescenceFriday! Our #CellLineOfTheWeek is Alpha Actinin 2! 🫀🧫

Our stem cell lines and plasmids are fluorescently-tagged to illuminate structures and available for just the cost of shipping.

📦 Distributed by @coriellinstitute.bsky.social and @addgene.bsky.social

#OpenScience
October 17, 2025 at 8:17 PM
natcomms.nature.com
Dissecting the fitness effects of a carbapenem resistance plasmid in clinical Enterobacterales using plasmid-wide CRISPRi screens

@aliciapcv.bsky.social @jorgesastred.bsky.social @sanmillan.bsky.social @cnb-csic.bsky.social

#bacteria #plasmids
Dissecting pOXA-48 fitness effects in clinical Enterobacterales using plasmid-wide CRISPRi screens - Nature Communications
This study investigates the effects of the carbapenem resistance plasmid pOXA-48 in clinical enterobacteria. Using CRISPRi screens, the authors revealed that the carbapenemase OXA-48 has a role in the plasmid-associated fitness costs.
bit.ly
October 17, 2025 at 4:56 PM
misssalmonella.bsky.social
🧬 What if #plasmids weren’t competitors, but cooperators?
A new study in npj Antimicrobials & Resistance shows that many plasmids coexist within the same bacterium — and that non-resistant ones survive thanks to those carrying #AMR genes.
#AMR #Plasmids #OneHealth
🔗 doi.org/10.1038/s442...
Communities of plasmids as strategies for antimicrobial resistance gene survival in wastewater treatment plant effluent - npj Antimicrobials and Resistance
npj Antimicrobials and Resistance - Communities of plasmids as strategies for antimicrobial resistance gene survival in wastewater treatment plant effluent
doi.org
October 15, 2025 at 6:14 PM