Reposted by Wim Hagen
Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit
www.nexperion.net/semcm2026
www.nexperion.net/semcm2026
September 23, 2025 at 10:42 AM
Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit
www.nexperion.net/semcm2026
www.nexperion.net/semcm2026
Reposted by Wim Hagen
For something different, tried this approach on something on the other end of the spectrum - in situ ribosomes from low SNR 2DTM data.
Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...).
Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å
Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...).
Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å
September 19, 2025 at 1:36 PM
For something different, tried this approach on something on the other end of the spectrum - in situ ribosomes from low SNR 2DTM data.
Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...).
Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å
Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...).
Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å
The Dutch mountains…
September 14, 2025 at 3:39 PM
The Dutch mountains…
Finally, an FSC discussion again! It’s been a while…..
Oops, have I just inadvertently opened up a new FSC-can-of-worms on the @ccpem.bsky.social mailing list.....? 😱
July 18, 2025 at 12:42 PM
Finally, an FSC discussion again! It’s been a while…..
Reposted by Wim Hagen
Whoever gets the resolution of raw tomograms to <10 Å within the sampled parts of Fourier space would probably deserve a Nobel. Or they'd get one anyway for the crazy new physics they discovered to achieve that.
“Achieving individual tomograms of cells where any features are at 10 Å resolution or better is well-beyond both current capabilities and reasonable predictions of future improvements.”
:(
:(
Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...
July 1, 2025 at 6:31 PM
Whoever gets the resolution of raw tomograms to <10 Å within the sampled parts of Fourier space would probably deserve a Nobel. Or they'd get one anyway for the crazy new physics they discovered to achieve that.
Reposted by Wim Hagen
Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...
To tilt or not to tilt? Strategies for in situ cryo-EM data collection
Recent breakthroughs in single-particle cryogenic electron microscopy (cryo-EM) and protein structure prediction have transformed our ability to resol…
www.sciencedirect.com
June 28, 2025 at 2:04 PM
Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...
Last day of a fantastic Inserm tomography workshop in Bordeaux.
May 21, 2025 at 7:54 AM
Last day of a fantastic Inserm tomography workshop in Bordeaux.
Oranjerie Roermond tonight.
May 3, 2025 at 5:18 PM
Oranjerie Roermond tonight.
Cold showers and warm beer the rest of the week at CCPEM!
April 22, 2025 at 5:49 PM
Cold showers and warm beer the rest of the week at CCPEM!
Mark? @subangstrom.bsky.social
When you have spent several weeks purifying your beloved sample and it felt at the bottom of the liquid nitrogen tank 😄 #CryoEM
December 17, 2024 at 6:03 PM
Mark? @subangstrom.bsky.social
Ever wondered how some of the automation in cryo-EM data collection works? Read Bram Koster's 1989 thesis!
repository.tudelft.nl/record/uuid:...
repository.tudelft.nl/record/uuid:...
Autotuning of a transmission electron microscope | TU Delft Repository
repository.tudelft.nl
December 17, 2024 at 4:21 PM
Ever wondered how some of the automation in cryo-EM data collection works? Read Bram Koster's 1989 thesis!
repository.tudelft.nl/record/uuid:...
repository.tudelft.nl/record/uuid:...
Reposted by Wim Hagen
Take a look at my personal perspective on the past, present, and future of in situ structural biology with cryo-ET+
While initially skeptical about the genre, I found it fun to write and hopefully, you can enjoy my experiences and some thoughts. Nice weekend y'all
www.cambridge.org/core/journal...
While initially skeptical about the genre, I found it fun to write and hopefully, you can enjoy my experiences and some thoughts. Nice weekend y'all
www.cambridge.org/core/journal...
The big chill: Growth of in situ structural biology with cryo-electron tomography | QRB Discovery | Cambridge Core
The big chill: Growth of in situ structural biology with cryo-electron tomography - Volume 5
www.cambridge.org
December 13, 2024 at 4:45 PM
Take a look at my personal perspective on the past, present, and future of in situ structural biology with cryo-ET+
While initially skeptical about the genre, I found it fun to write and hopefully, you can enjoy my experiences and some thoughts. Nice weekend y'all
www.cambridge.org/core/journal...
While initially skeptical about the genre, I found it fun to write and hopefully, you can enjoy my experiences and some thoughts. Nice weekend y'all
www.cambridge.org/core/journal...
Reposted by Wim Hagen
Looking for a primer on why the contrast transfer function matters in #cryoEM?
Have a look at our updated #CryoSPARC Guide pages on CTF estimation! We cover intuition, theory, job types and practical considerations: guide.cryosparc.com/processing-d...
Have a look at our updated #CryoSPARC Guide pages on CTF estimation! We cover intuition, theory, job types and practical considerations: guide.cryosparc.com/processing-d...
December 9, 2024 at 9:24 PM
Looking for a primer on why the contrast transfer function matters in #cryoEM?
Have a look at our updated #CryoSPARC Guide pages on CTF estimation! We cover intuition, theory, job types and practical considerations: guide.cryosparc.com/processing-d...
Have a look at our updated #CryoSPARC Guide pages on CTF estimation! We cover intuition, theory, job types and practical considerations: guide.cryosparc.com/processing-d...
Phase plates: what do we expect from the next attempt, the laser phase plate?
November 29, 2024 at 11:37 PM
Phase plates: what do we expect from the next attempt, the laser phase plate?