@willarnold.bsky.social
Bioinformatics Scientist in Gene&Cell Therapy. Leveraging NGS to drive better genetic therapies.
Former @bstevensonlab.bsky.social and @addgene.bsky.social. Current ElevateBio. Opinions/views my own.
Former @bstevensonlab.bsky.social and @addgene.bsky.social. Current ElevateBio. Opinions/views my own.
Reposted
"Stevenson hopes his work changes the drug treatment for Lyme disease. 'If these proposals never get reviewed, or if council meets too late, I won't have any money, and I’ll have to let people go,' he said. 'It’s very frustrating and very depressing.' ”
🦠🧫🧪🔬🧬
www.wbur.org/news/2025/02...
🦠🧫🧪🔬🧬
www.wbur.org/news/2025/02...
Canceled meetings and confusion: NIH grant funding in limbo despite court injunction
Science researchers awaiting National Institutes of Health funding say their grant meetings are being canceled, despite a court order blocking the Trump administration from freezing federal funds.
www.wbur.org
February 25, 2025 at 6:34 PM
"Stevenson hopes his work changes the drug treatment for Lyme disease. 'If these proposals never get reviewed, or if council meets too late, I won't have any money, and I’ll have to let people go,' he said. 'It’s very frustrating and very depressing.' ”
🦠🧫🧪🔬🧬
www.wbur.org/news/2025/02...
🦠🧫🧪🔬🧬
www.wbur.org/news/2025/02...
Reposted
Cool “Behind the paper” article on our recent KorB-KorA sliding clamp-locking clamp story. I remember Chris Thomas and Lewis Bingle (Birmingham) taught me about these proteins when I was an undergrad, and I kept remembering they said the mechanism was not clear!!!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Sliding clamp protein enables long-range gene silencing in a bacterial plasmid - Nature Microbiology
Long-distance gene regulation is uncommon in bacteria, and its molecular mechanisms are unclear. Using a combination of structural, biochemical and single-molecule techniques, researchers revealed tha...
www.nature.com
February 4, 2025 at 6:10 AM
Cool “Behind the paper” article on our recent KorB-KorA sliding clamp-locking clamp story. I remember Chris Thomas and Lewis Bingle (Birmingham) taught me about these proteins when I was an undergrad, and I kept remembering they said the mechanism was not clear!!!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted
Targeted decontamination of sequencing data with CLEAN https://www.biorxiv.org/content/10.1101/2023.08.05.552089v3 🧬🖥️🧪 Nextflow: https://github.com/rki-mf1/clean
January 25, 2025 at 10:39 AM
Targeted decontamination of sequencing data with CLEAN https://www.biorxiv.org/content/10.1101/2023.08.05.552089v3 🧬🖥️🧪 Nextflow: https://github.com/rki-mf1/clean
Reposted
1/ 🔬 Our work, "Dynamic transitions of initiator binding coordinate the replication of the two chromosomes in Vibrio cholerae", is now published in Nature Communications.
Here's a thread on how we think Chr1 and Chr2 replication is coordinated in Vibrio. 🧵
Link : www.nature.com/articles/s41...
Here's a thread on how we think Chr1 and Chr2 replication is coordinated in Vibrio. 🧵
Link : www.nature.com/articles/s41...
January 9, 2025 at 12:32 PM
1/ 🔬 Our work, "Dynamic transitions of initiator binding coordinate the replication of the two chromosomes in Vibrio cholerae", is now published in Nature Communications.
Here's a thread on how we think Chr1 and Chr2 replication is coordinated in Vibrio. 🧵
Link : www.nature.com/articles/s41...
Here's a thread on how we think Chr1 and Chr2 replication is coordinated in Vibrio. 🧵
Link : www.nature.com/articles/s41...
Reposted
Today's holiday ☕ reading is this 2024 paper on PARIS, a bacterial defense system shaped like a literal propeller that responds to viral infection by hitting the cellular self-destruct button - cleaving an essential tRNA. www.nature.com/articles/s41...
A virally encoded tRNA neutralizes the PARIS antiviral defence system - Nature
Structural and functional studies reveal how viral proteins trigger the phage antirestriction induced system (PARIS) to degrade host tRNA and how viral tRNAs suppress the PARIS nuclease and thereby ov...
www.nature.com
December 24, 2024 at 3:18 PM
Today's holiday ☕ reading is this 2024 paper on PARIS, a bacterial defense system shaped like a literal propeller that responds to viral infection by hitting the cellular self-destruct button - cleaving an essential tRNA. www.nature.com/articles/s41...
Reposted
The total length of human DNA on the planet right now is thus: (3.4x10^-10) • (2 x 3.055x10^9) • (3x10^13) • (8.2x10^9) = 5.11 x 10^23 meters
Time to call in NASA!
1 *light-year* – the distance light travels in one year – is 9.46x10^15 meters...
So: 54 million light-years of human DNA!
Time to call in NASA!
1 *light-year* – the distance light travels in one year – is 9.46x10^15 meters...
So: 54 million light-years of human DNA!
December 8, 2024 at 9:52 PM
The total length of human DNA on the planet right now is thus: (3.4x10^-10) • (2 x 3.055x10^9) • (3x10^13) • (8.2x10^9) = 5.11 x 10^23 meters
Time to call in NASA!
1 *light-year* – the distance light travels in one year – is 9.46x10^15 meters...
So: 54 million light-years of human DNA!
Time to call in NASA!
1 *light-year* – the distance light travels in one year – is 9.46x10^15 meters...
So: 54 million light-years of human DNA!
Reposted
Love seeing what's new in the Addgene repository? So do we!
Check out our latest 🔥Hot Plasmids 🔥 post to see five Addgenie's picks for what's new and hot in our plasmid (and antibody!) repository this fall.
🧪🧬
blog.addgene.org/hot...
243 / 300
Hot Plasmids: Fall 2024
Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!
blog.addgene.org
December 4, 2024 at 1:03 PM
Love seeing what's new in the Addgene repository? So do we!
Check out our latest 🔥Hot Plasmids 🔥 post to see five Addgenie's picks for what's new and hot in our plasmid (and antibody!) repository this fall.
🧪🧬
blog.addgene.org/hot...
243 / 300
Reposted
Our new preprint is out - please share!
We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.
Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting
We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.
Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting
December 2, 2024 at 3:16 PM
Our new preprint is out - please share!
We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.
Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting
We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.
Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting
Reposted
It's cool in the RNA modifications world systematic basecalling errors are one strategy to identify modified sites. Kind of a hacky (and I'm sure has false positives) approach but appealing in its simplicity.
It was also done for Phosphorothioated DNA here: www.frontiersin.org/journals/mic...
It was also done for Phosphorothioated DNA here: www.frontiersin.org/journals/mic...
November 30, 2024 at 4:20 PM
It's cool in the RNA modifications world systematic basecalling errors are one strategy to identify modified sites. Kind of a hacky (and I'm sure has false positives) approach but appealing in its simplicity.
It was also done for Phosphorothioated DNA here: www.frontiersin.org/journals/mic...
It was also done for Phosphorothioated DNA here: www.frontiersin.org/journals/mic...
Reposted
I’m sharing again the link to the Genomics Core Facilities starter pack that I put together as I added more accounts and feeds today go.bsky.app/HiWxZZc
November 27, 2024 at 9:04 PM
I’m sharing again the link to the Genomics Core Facilities starter pack that I put together as I added more accounts and feeds today go.bsky.app/HiWxZZc
Reposted
Chromatin context-dependent regulation and epigenetic manipulation of prime editing
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
Chromatin context-dependent regulation and epigenetic manipulation of prime editing
Prime editing exhibits strong, chromatin-related position effects and is promoted
by active transcriptional elongation. Leveraging these features via epigenetic conditioning
of a locus modulates prime...
www.cell.com
November 23, 2024 at 11:23 PM
Chromatin context-dependent regulation and epigenetic manipulation of prime editing
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...