B Thuronyi (they/them)
@thuronyi.bsky.social
Synthetic biologist, V. natriegens booster, Sheets torturer & asst prof Chem @ Williams College
Pinned
CloneCoordinate: Open-Source Software for Collaborative DNA Construction
Custom DNA constructs have never been more common or important in the life sciences. Many researchers therefore devote substantial time and effort to molecular cloning, aided by abundant computer-aide...
pubs.acs.org
Delighted to report that our group's passion project over the last 6+ years to make DNA cloning more accessible, efficient, and scalable using a software-assisted workflow called CloneCoordinate is now out in ACS Synbio!
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Reposted by B Thuronyi (they/them)
In the context of Thanksgiving (and in general), I am always amazed by how hard the American mainstream try to erase/ignore the history of colonialism, and how the first successful harvest in the colony was followed by the eventual genocide of the Native Americans.
November 27, 2025 at 2:08 PM
In the context of Thanksgiving (and in general), I am always amazed by how hard the American mainstream try to erase/ignore the history of colonialism, and how the first successful harvest in the colony was followed by the eventual genocide of the Native Americans.
It's so great to see folks signing up to try CC!
There's a demo version (at clonecoordinate.org) if you want to play with it, and the full, blank version is behind a short all-questions-optional form.
I especially encourage people to join the Slack so I can help you with onboarding questions!
There's a demo version (at clonecoordinate.org) if you want to play with it, and the full, blank version is behind a short all-questions-optional form.
I especially encourage people to join the Slack so I can help you with onboarding questions!
November 26, 2025 at 1:58 PM
It's so great to see folks signing up to try CC!
There's a demo version (at clonecoordinate.org) if you want to play with it, and the full, blank version is behind a short all-questions-optional form.
I especially encourage people to join the Slack so I can help you with onboarding questions!
There's a demo version (at clonecoordinate.org) if you want to play with it, and the full, blank version is behind a short all-questions-optional form.
I especially encourage people to join the Slack so I can help you with onboarding questions!
Reposted by B Thuronyi (they/them)
#OpenCloning is a an Open Source alternative to SnapGene/Benchling that supports automation and integration with other software
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
November 24, 2025 at 4:57 PM
#OpenCloning is a an Open Source alternative to SnapGene/Benchling that supports automation and integration with other software
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
Reposted by B Thuronyi (they/them)
#microsky #phagesky #phage
Anyone who’s tried deleting prophages in the lab by HR knows the difficulties of the task. Here we have an example of how HR-mediated natural transformation might hit the same hurdle in a more native context.
academic.oup.com/mbe/article/...
Anyone who’s tried deleting prophages in the lab by HR knows the difficulties of the task. Here we have an example of how HR-mediated natural transformation might hit the same hurdle in a more native context.
academic.oup.com/mbe/article/...
Chromosomal Curing Drives an Arms Race Between Bacterial Transformation and Prophage
Abstract. Transformation occurs when bacteria import exogenous DNA via the competence machinery and integrate it into their genome through homologous recom
academic.oup.com
November 23, 2025 at 4:39 PM
#microsky #phagesky #phage
Anyone who’s tried deleting prophages in the lab by HR knows the difficulties of the task. Here we have an example of how HR-mediated natural transformation might hit the same hurdle in a more native context.
academic.oup.com/mbe/article/...
Anyone who’s tried deleting prophages in the lab by HR knows the difficulties of the task. Here we have an example of how HR-mediated natural transformation might hit the same hurdle in a more native context.
academic.oup.com/mbe/article/...
Reposted by B Thuronyi (they/them)
this looks like a dream come true for my lab. Can't wait to try it out!
November 21, 2025 at 9:52 PM
this looks like a dream come true for my lab. Can't wait to try it out!
Reposted by B Thuronyi (they/them)
A huge effort finally coming to fruition for B and their group. So excited to give this a whirl esp now that my cloning projects have ramped up substantially. Check this out 👇👇
Delighted to report that our group's passion project over the last 6+ years to make DNA cloning more accessible, efficient, and scalable using a software-assisted workflow called CloneCoordinate is now out in ACS Synbio!
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
CloneCoordinate: Open-Source Software for Collaborative DNA Construction
Custom DNA constructs have never been more common or important in the life sciences. Many researchers therefore devote substantial time and effort to molecular cloning, aided by abundant computer-aide...
pubs.acs.org
November 21, 2025 at 9:43 PM
A huge effort finally coming to fruition for B and their group. So excited to give this a whirl esp now that my cloning projects have ramped up substantially. Check this out 👇👇
I seem to be unspammed, so that was only a few hours -- yay!
A warm welcome from Bluesky, labeled as a spam account after writing 1 thread!!
November 21, 2025 at 9:22 PM
I seem to be unspammed, so that was only a few hours -- yay!
A warm welcome from Bluesky, labeled as a spam account after writing 1 thread!!
November 21, 2025 at 7:42 PM
A warm welcome from Bluesky, labeled as a spam account after writing 1 thread!!
Delighted to report that our group's passion project over the last 6+ years to make DNA cloning more accessible, efficient, and scalable using a software-assisted workflow called CloneCoordinate is now out in ACS Synbio!
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
CloneCoordinate: Open-Source Software for Collaborative DNA Construction
Custom DNA constructs have never been more common or important in the life sciences. Many researchers therefore devote substantial time and effort to molecular cloning, aided by abundant computer-aide...
pubs.acs.org
November 21, 2025 at 7:40 PM
Delighted to report that our group's passion project over the last 6+ years to make DNA cloning more accessible, efficient, and scalable using a software-assisted workflow called CloneCoordinate is now out in ACS Synbio!
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...