B Thuronyi (they/them)
@thuronyi.bsky.social
Synthetic biologist, V. natriegens booster, Sheets torturer & asst prof Chem @ Williams College
Little disappointing they chose the word "overwhelmed" at the opening -- seems inaccurate! -- but a great piece overall!
I appreciate your raising the profile of this kind of awful behavior -- it's useful for everyone to see how extreme it still is in some settings
I appreciate your raising the profile of this kind of awful behavior -- it's useful for everyone to see how extreme it still is in some settings
November 25, 2025 at 12:41 PM
Little disappointing they chose the word "overwhelmed" at the opening -- seems inaccurate! -- but a great piece overall!
I appreciate your raising the profile of this kind of awful behavior -- it's useful for everyone to see how extreme it still is in some settings
I appreciate your raising the profile of this kind of awful behavior -- it's useful for everyone to see how extreme it still is in some settings
It's just funny to me that this is the one situation where the consumers of LoRs are also producers (if for different opportunities) so you'd think they'd have incentive to streamline
November 25, 2025 at 10:56 AM
It's just funny to me that this is the one situation where the consumers of LoRs are also producers (if for different opportunities) so you'd think they'd have incentive to streamline
Has there ever been any momentum behind a "common app" for grad school LoRs? Like, generic letter only, student gets to direct where it goes - I think Interfolio can do it this way - and then maybe an optional 500 character text box for us for school-specific comments? Not the cycle for it, but...
November 25, 2025 at 10:54 AM
Has there ever been any momentum behind a "common app" for grad school LoRs? Like, generic letter only, student gets to direct where it goes - I think Interfolio can do it this way - and then maybe an optional 500 character text box for us for school-specific comments? Not the cycle for it, but...
Marketing is very important! The inertia of previously established methods -- and even previously established GAPS, i.e. "I know that's not doable so I'm not interested in trying" -- is enormous...
November 23, 2025 at 6:56 PM
Marketing is very important! The inertia of previously established methods -- and even previously established GAPS, i.e. "I know that's not doable so I'm not interested in trying" -- is enormous...
Thank you for bringing this energy
November 22, 2025 at 12:31 AM
Thank you for bringing this energy
Hiii!! Thank you! It's just B now! I'm in the last year before tenure submission and sweating bullets but it'll probably all be ok......
Hope you're doing good too!
Hope you're doing good too!
November 22, 2025 at 12:10 AM
Hiii!! Thank you! It's just B now! I'm in the last year before tenure submission and sweating bullets but it'll probably all be ok......
Hope you're doing good too!
Hope you're doing good too!
Woohoo! Let me know how it goes! If you join the Slack we can help with onboarding
November 21, 2025 at 10:14 PM
Woohoo! Let me know how it goes! If you join the Slack we can help with onboarding
Reposted by B Thuronyi (they/them)
this looks like a dream come true for my lab. Can't wait to try it out!
November 21, 2025 at 9:52 PM
this looks like a dream come true for my lab. Can't wait to try it out!
Would love to have some boosts to help with visibility for this project as I start from scratch here on Bluesky... great to see some old friends like @atinygreencell.bsky.social here and I hope to rediscover more!
November 21, 2025 at 7:40 PM
Would love to have some boosts to help with visibility for this project as I start from scratch here on Bluesky... great to see some old friends like @atinygreencell.bsky.social here and I hope to rediscover more!
Let's make it easier to document what we're doing so we can learn from our own and others' experiences with cloning and all the many operations and decisions involved in handling DNA.
clonecoordinate.org
clonecoordinate.org
CloneCoordinate | Free, open source lab-scale DNA foundry software for collaborative cloning
clonecoordinate.org
November 21, 2025 at 7:40 PM
Let's make it easier to document what we're doing so we can learn from our own and others' experiences with cloning and all the many operations and decisions involved in handling DNA.
clonecoordinate.org
clonecoordinate.org
Building DNA that's never existed before is still central to synbio and lots of life sciences. No matter how much you outsource, there's still tons to keep track of and manage, and lots of projects still get hung up on cloning!
November 21, 2025 at 7:40 PM
Building DNA that's never existed before is still central to synbio and lots of life sciences. No matter how much you outsource, there's still tons to keep track of and manage, and lots of projects still get hung up on cloning!
Yes, DNA synthesis is getting cheaper and easier and cloning as a fee-for-service is increasingly common. Yes, @plasmidsaurus.bsky.social is making it easier than ever to verify your materials and find out how your assemblies went wrong. (thanks @dna.bsky.social!)
BUT...
BUT...
November 21, 2025 at 7:40 PM
Yes, DNA synthesis is getting cheaper and easier and cloning as a fee-for-service is increasingly common. Yes, @plasmidsaurus.bsky.social is making it easier than ever to verify your materials and find out how your assemblies went wrong. (thanks @dna.bsky.social!)
BUT...
BUT...
Cloning at scale at a primarily, or exclusively, undergrad institution is very possible with the right tools. Now that we have those, we want to share them with everyone! CC let us work as a collaborative team, even across generations of students.
November 21, 2025 at 7:40 PM
Cloning at scale at a primarily, or exclusively, undergrad institution is very possible with the right tools. Now that we have those, we want to share them with everyone! CC let us work as a collaborative team, even across generations of students.
We've cloned four hundred or so constructs over the last 5 years (a bunch of them recently published with @fritzlab.bsky.social as part of the Vnat Collection) with an undergrad-only team *while* we built CC from scratch...
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
Expanding genetic engineering capabilities in Vibrio natriegens with the Vnat Collection
Abstract. Vibrio natriegens, with its exceptionally fast growth rate, has great promise as a revolutionary chassis for synthetic biology, yet the realizati
academic.oup.com
November 21, 2025 at 7:40 PM
We've cloned four hundred or so constructs over the last 5 years (a bunch of them recently published with @fritzlab.bsky.social as part of the Vnat Collection) with an undergrad-only team *while* we built CC from scratch...
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
CC is a framework for recording the granular details of DNA assembly (in familiar spreadsheet format) so they can be part of publishing -- joining other neglected elements like, uh, construct sequences?? (as I wrote w/@erika-alden.bsky.social and Jeff Barrick):
journals.plos.org/plosbiology/...
journals.plos.org/plosbiology/...
No assembly required: Time for stronger, simpler publishing standards for DNA sequences
Describing how DNA constructs were assembled is no longer necessary when it is possible to fully sequence the final results to validate them. In this Perspective, the authors suggest that outdated met...
journals.plos.org
November 21, 2025 at 7:40 PM
CC is a framework for recording the granular details of DNA assembly (in familiar spreadsheet format) so they can be part of publishing -- joining other neglected elements like, uh, construct sequences?? (as I wrote w/@erika-alden.bsky.social and Jeff Barrick):
journals.plos.org/plosbiology/...
journals.plos.org/plosbiology/...
If you're a responsible researcher who wants their carefully collected data to benefit the broadest possible community, why not document the details of how you MAKE all those constructs you're testing so you can share that valuable experience with others?
November 21, 2025 at 7:40 PM
If you're a responsible researcher who wants their carefully collected data to benefit the broadest possible community, why not document the details of how you MAKE all those constructs you're testing so you can share that valuable experience with others?
If you've thought about working cloning into undergrad research or summer projects or teaching labs or your iGEM @igemhq.bsky.social team but it feels like an uphill battle getting everyone up to speed, keeping them on the same page, and having them work productively as a unit – consider CC!
November 21, 2025 at 7:40 PM
If you've thought about working cloning into undergrad research or summer projects or teaching labs or your iGEM @igemhq.bsky.social team but it feels like an uphill battle getting everyone up to speed, keeping them on the same page, and having them work productively as a unit – consider CC!
If you're in the early stages of starting a lab (hang in there!!) this is a great time to start off on the right foot with a highly organized system that will make sure as little as possible slips through the cracks and take a lot of cognitive load off your team.
November 21, 2025 at 7:40 PM
If you're in the early stages of starting a lab (hang in there!!) this is a great time to start off on the right foot with a highly organized system that will make sure as little as possible slips through the cracks and take a lot of cognitive load off your team.
We all have our takes on what makes cloning work well or poorly. We might all be right: every lab's techniques and constructs are different, so it seems like the best way to go is use data about YOUR cloning to make decisions about how to do YOUR cloning.
November 21, 2025 at 7:40 PM
We all have our takes on what makes cloning work well or poorly. We might all be right: every lab's techniques and constructs are different, so it seems like the best way to go is use data about YOUR cloning to make decisions about how to do YOUR cloning.
Even with my years of training I still couldn't keep the progress and to-do list for more than a dozen or so constructs organized at once. Now I just outsource all that to a computer and focus on the science.
November 21, 2025 at 7:40 PM
Even with my years of training I still couldn't keep the progress and to-do list for more than a dozen or so constructs organized at once. Now I just outsource all that to a computer and focus on the science.