Taylor Cunliffe
@taylorcunliffe.bsky.social
Final year PhD candidate in the Heras Lab at the La Trobe Institute for Molecular Science in Melbourne 🧬 Structural biologist studying the roles of disulphide bond formation proteins in bacteria 🧫
Reposted by Taylor Cunliffe
Type VII ABC transporters underpin many core activities in the bacterial cell envelope - including efflux, cell division and lipoprotein trafficking.
A new paper in PLOS Biology reveals YbbAP-TesA as a novel Type VII system in E. coli.
dx.plos.org/10.1371/jour...
A new paper in PLOS Biology reveals YbbAP-TesA as a novel Type VII system in E. coli.
dx.plos.org/10.1371/jour...
Structural characterization of the YbbAP-TesA ABC transporter identifies it as a lipid hydrolase complex that extracts hydrophobic compounds from the bacterial inner membrane
In E.coli, three of the four Type VII ABC transporter systems have been structurally characterized. This study solves cryo-EM structures of the fourth Type VII ABC system, YbbAP-TesA, and suggests tha...
dx.plos.org
November 26, 2025 at 12:46 PM
Type VII ABC transporters underpin many core activities in the bacterial cell envelope - including efflux, cell division and lipoprotein trafficking.
A new paper in PLOS Biology reveals YbbAP-TesA as a novel Type VII system in E. coli.
dx.plos.org/10.1371/jour...
A new paper in PLOS Biology reveals YbbAP-TesA as a novel Type VII system in E. coli.
dx.plos.org/10.1371/jour...
Reposted by Taylor Cunliffe
Very happy to share our work on lipopolysaccharide assembly by the Lpt complex, published today in @natcomms.nature.com
Fantastic collaboration with @raffaeleieva.bsky.social l and t @pstansfeld.bsky.social
Congrats to all authors, especially Haoxiang, Axel & Violette
🔗 doi.org/10.1038/s414...
Fantastic collaboration with @raffaeleieva.bsky.social l and t @pstansfeld.bsky.social
Congrats to all authors, especially Haoxiang, Axel & Violette
🔗 doi.org/10.1038/s414...
Structural basis of lipopolysaccharide assembly by the outer membrane translocon holo-complex - Nature Communications
The asymmetric distribution of lipopolysaccharide (LPS) on the surface of the bacterial outer membrane is essential and crucial for antibiotic resistance. Here, authors characterize the LPS translocon...
doi.org
November 24, 2025 at 3:22 PM
Very happy to share our work on lipopolysaccharide assembly by the Lpt complex, published today in @natcomms.nature.com
Fantastic collaboration with @raffaeleieva.bsky.social l and t @pstansfeld.bsky.social
Congrats to all authors, especially Haoxiang, Axel & Violette
🔗 doi.org/10.1038/s414...
Fantastic collaboration with @raffaeleieva.bsky.social l and t @pstansfeld.bsky.social
Congrats to all authors, especially Haoxiang, Axel & Violette
🔗 doi.org/10.1038/s414...
Reposted by Taylor Cunliffe
1/9 Metagenomics lets us read microbiomes in nature without cultivation, but writing (editing) them in their native context is still a major challenge.
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
Although metagenomic sequencing has revealed a rich microbial biodiversity in the mammalian gut, methods to genetically alter specific species in the microbiome are highly limited. Here, we introduce ...
science.org
November 14, 2025 at 2:25 PM
1/9 Metagenomics lets us read microbiomes in nature without cultivation, but writing (editing) them in their native context is still a major challenge.
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Reposted by Taylor Cunliffe
Our latest work on the nitrogenase-like methylthio-alkane reductase, which specifically reduces reduces carbon-sulfide bonds is now out @natcatal.nature.com: doi.org/10.1038/s419.... We find for the first time large #nitrogenase metalloclusters (P- and L-cluster) outside nitrogenases.
October 23, 2025 at 10:09 AM
Our latest work on the nitrogenase-like methylthio-alkane reductase, which specifically reduces reduces carbon-sulfide bonds is now out @natcatal.nature.com: doi.org/10.1038/s419.... We find for the first time large #nitrogenase metalloclusters (P- and L-cluster) outside nitrogenases.
Reposted by Taylor Cunliffe
🧐Checkout our preprint revealing the stepwise firing💥 mechanism of a Contractile Injection System @xujwet.bsky.social&@chipericson.bsky.social trapped the complex by structure-guided engineering🧪 in multiple intermediate states and imaged them by multimodal #cryoEM❄️🔬
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
October 8, 2025 at 9:18 PM
🧐Checkout our preprint revealing the stepwise firing💥 mechanism of a Contractile Injection System @xujwet.bsky.social&@chipericson.bsky.social trapped the complex by structure-guided engineering🧪 in multiple intermediate states and imaged them by multimodal #cryoEM❄️🔬
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Taylor Cunliffe
The outer membrane of Gram-negative bacteria blocks many antibiotics. Our latest work reveals that L-type pyocins bypass this barrier by inactivating the BAM complex, killing Pseudomonas aeruginosa without entering the cell, providing a new blueprint for beating antibiotic resistance.
A Protein Antibiotic Inhibits the BAM Complex to Kill Without Cell Entry
Many antibiotics are ineffective against Gram-negative pathogens such as Pseudomonas aeruginosa because they cannot penetrate the bacterial outer membrane. Here, we show that protein antibiotics calle...
www.biorxiv.org
September 20, 2025 at 9:29 AM
The outer membrane of Gram-negative bacteria blocks many antibiotics. Our latest work reveals that L-type pyocins bypass this barrier by inactivating the BAM complex, killing Pseudomonas aeruginosa without entering the cell, providing a new blueprint for beating antibiotic resistance.
Reposted by Taylor Cunliffe
Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
August 27, 2025 at 4:14 PM
Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Taylor Cunliffe
SPEAR (bacterial nanosynringe) enables loading of different types of cargo (including folded ribonucleoproteins and single-stranded DNA) and targeting of defined cell types both in vitro and in vivo
www.nature.com/articles/s41...
@natbiotech.nature.com @zhangf.bsky.social
www.nature.com/articles/s41...
@natbiotech.nature.com @zhangf.bsky.social
August 12, 2025 at 7:00 PM
SPEAR (bacterial nanosynringe) enables loading of different types of cargo (including folded ribonucleoproteins and single-stranded DNA) and targeting of defined cell types both in vitro and in vivo
www.nature.com/articles/s41...
@natbiotech.nature.com @zhangf.bsky.social
www.nature.com/articles/s41...
@natbiotech.nature.com @zhangf.bsky.social
Reposted by Taylor Cunliffe
1/3 Electricity from air! In the top journal Nano Energy today, we report the first fuel cell that makes power from air alone. We achieved this by using the enzyme Huc to oxidize ambient hydrogen. This results in a continuous low-level power from air and higher outputs with industrial waste gases.
Nanoengineered bioanode with oxygen-insensitive hydrogenase for sustainable energy harvesting from atmospheric hydrogen and waste gases
Enzymatic biofuel cells (EBFCs) utilizing hydrogenases to oxidize hydrogen offer a sustainable approach to energy conservation. However, the oxygen se…
www.sciencedirect.com
August 8, 2025 at 3:49 AM
1/3 Electricity from air! In the top journal Nano Energy today, we report the first fuel cell that makes power from air alone. We achieved this by using the enzyme Huc to oxidize ambient hydrogen. This results in a continuous low-level power from air and higher outputs with industrial waste gases.
Reposted by Taylor Cunliffe
📣 New paper alert! Just out in Cell Reports! pubmed.ncbi.nlm.nih.gov/40644298/
Thrilled to share that we have discovered a brand-new anti-phage defense system! Bacteria have evolved various defense strategies (CRISPR etc) to counter phage attacks. We found a new one - fascinating and dramatic
⚔️🦠❄️🔬
Thrilled to share that we have discovered a brand-new anti-phage defense system! Bacteria have evolved various defense strategies (CRISPR etc) to counter phage attacks. We found a new one - fascinating and dramatic
⚔️🦠❄️🔬
August 2, 2025 at 3:01 PM
📣 New paper alert! Just out in Cell Reports! pubmed.ncbi.nlm.nih.gov/40644298/
Thrilled to share that we have discovered a brand-new anti-phage defense system! Bacteria have evolved various defense strategies (CRISPR etc) to counter phage attacks. We found a new one - fascinating and dramatic
⚔️🦠❄️🔬
Thrilled to share that we have discovered a brand-new anti-phage defense system! Bacteria have evolved various defense strategies (CRISPR etc) to counter phage attacks. We found a new one - fascinating and dramatic
⚔️🦠❄️🔬
Reposted by Taylor Cunliffe
February 11, 2025 at 1:14 AM