Johannes Rebelein
@rebeleinlab.bsky.social
Nitrogenase, Biotic and Artificial Metalloenzymes, CO2 and N2 Fixation, Emmy Noether Research Group Leader at the Max Planck Institute for Terrestrial Microbiology: https://www.mpi-marburg.mpg.de/859249/Johannes-Rebelein
Very cool story about the efficient transport and incorporation of non canonical amino acid!
🚨Our paper is out! 🥳
Hijacking a bacterial ABC transporter for efficient genetic code expansion.
Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social
www.nature.com/articles/s41...
it all started years ago with a failed experiment
🧵👇 1/9
Hijacking a bacterial ABC transporter for efficient genetic code expansion.
Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social
www.nature.com/articles/s41...
it all started years ago with a failed experiment
🧵👇 1/9
Hijacking a bacterial ABC transporter for genetic code expansion - Nature
Bacterial ATP-binding cassette (ABC) transporters can be utilized and engineered to transport non-canonical amino acids into Escherichia coli for highly efficient synthesis of proteins with novel func...
www.nature.com
November 1, 2025 at 1:52 PM
Very cool story about the efficient transport and incorporation of non canonical amino acid!
Our latest work on the nitrogenase-like methylthio-alkane reductase, which specifically reduces reduces carbon-sulfide bonds is now out @natcatal.nature.com: doi.org/10.1038/s419.... We find for the first time large #nitrogenase metalloclusters (P- and L-cluster) outside nitrogenases.
October 23, 2025 at 10:09 AM
Our latest work on the nitrogenase-like methylthio-alkane reductase, which specifically reduces reduces carbon-sulfide bonds is now out @natcatal.nature.com: doi.org/10.1038/s419.... We find for the first time large #nitrogenase metalloclusters (P- and L-cluster) outside nitrogenases.
Fantastic work by the Ward lab to repurpose cytochrome #P450 enzymes for a new-to-nature asymmetric metal-catalysed H atom transfer! Just released
@Nature
: doi.org/10.1038/s415.... A must read!🧪
@Nature
: doi.org/10.1038/s415.... A must read!🧪
Repurposing haemoproteins for asymmetric metal-catalysed H atom transfer - Nature
Cytochrome P450 enzymes can be repurposed to catalyse asymmetric metal–hydride hydrogen atom transfer, a new-to-nature reaction.
doi.org
July 31, 2025 at 11:37 PM
Fantastic work by the Ward lab to repurpose cytochrome #P450 enzymes for a new-to-nature asymmetric metal-catalysed H atom transfer! Just released
@Nature
: doi.org/10.1038/s415.... A must read!🧪
@Nature
: doi.org/10.1038/s415.... A must read!🧪
Great collaboration with @lennartschada.bsky.social on an #enzyme converting the #plastic component ethylene glycol and to a feedstock chemical for microbes. Offering new ways to use plastic biotechnologically. 🧪
Ethylene glycol is widely used as monomer of the plastic PET. It is therefore highly relevant as sustainable feedstock for microbial biotechnology. In our new paper in @natcomms.nature.com, we characterize efficient enzymes for ethylene glycol oxidation: www.nature.com/articles/s41...
July 3, 2025 at 12:25 PM
Great collaboration with @lennartschada.bsky.social on an #enzyme converting the #plastic component ethylene glycol and to a feedstock chemical for microbes. Offering new ways to use plastic biotechnologically. 🧪
I am honored and excited that our research on #nitrogenases and the conversion of #CO2 to hydrocarbons is highlighted in the Yearbook 2024 of the Max Planck Society, see page 29. An English and a German version of the Yearbook are available in www.mpg.de/13631298/yea....
July 3, 2025 at 12:18 PM
I am honored and excited that our research on #nitrogenases and the conversion of #CO2 to hydrocarbons is highlighted in the Yearbook 2024 of the Max Planck Society, see page 29. An English and a German version of the Yearbook are available in www.mpg.de/13631298/yea....
A must read for anybody interested in the evolution of life and how to construct phylogenies using structures. Outstanding work by @georghochberg.bsky.social and co-worker. Congrats!
New paper from the lab from Sriram Garg in my group. We introduce a general substitution matrix for structural phylogenetics. I think this is a big deal, so read on below if you think deep history is important. academic.oup.com/mbe/advance-...
A general substitution matrix for structural phylogenetics.
Abstract. Sequence-based maximum likelihood (ML) phylogenetics is a widely used method for inferring evolutionary relationships, which has illuminated the
academic.oup.com
June 13, 2025 at 2:19 PM
A must read for anybody interested in the evolution of life and how to construct phylogenies using structures. Outstanding work by @georghochberg.bsky.social and co-worker. Congrats!
Reposted by Johannes Rebelein
New paper from the lab from Sriram Garg in my group. We introduce a general substitution matrix for structural phylogenetics. I think this is a big deal, so read on below if you think deep history is important. academic.oup.com/mbe/advance-...
A general substitution matrix for structural phylogenetics.
Abstract. Sequence-based maximum likelihood (ML) phylogenetics is a widely used method for inferring evolutionary relationships, which has illuminated the
academic.oup.com
June 11, 2025 at 2:01 PM
New paper from the lab from Sriram Garg in my group. We introduce a general substitution matrix for structural phylogenetics. I think this is a big deal, so read on below if you think deep history is important. academic.oup.com/mbe/advance-...
Leveraging spectroscopic and structural approaches we obtained new insights into the role of #ferredoxins in N2 fixation. Just published in Chemistry – A European Journal: doi.org/10.1002/chem...! Great work by Holly Addison. Fantastic collaboration with Antonio Pierik on the EPR characterization.
Two Key Ferredoxins for Nitrogen Fixation Have Different Specificities and Biophysical Properties
Two ferredoxins, FdC and FdN, are collectively essential for the process of nitrogen fixation by the iron nitrogenase in R. capsulatus. We explore the biophysical factors, through spectroscopic and s....
doi.org
June 1, 2025 at 9:46 AM
Leveraging spectroscopic and structural approaches we obtained new insights into the role of #ferredoxins in N2 fixation. Just published in Chemistry – A European Journal: doi.org/10.1002/chem...! Great work by Holly Addison. Fantastic collaboration with Antonio Pierik on the EPR characterization.
Reposted by Johannes Rebelein
Marburg rules. I see @rebeleinlab.bsky.social @schullerjm.bsky.social @georghochberg.bsky.social in the crowd 😘
YES!!!Excellent initiative #Microbes-for-Climate #M4C🦠FUNDED💲💰☺️🤩
The best?🤔The people❤️
Endless gratitude to Tobi & Anke - amazing role models❤️ Love the "thanks BBQ no matter what" spirit from the "Präsidium"! @unimarburg.bsky.social - sure it tasted better as we got it😉😂 #ProfLife #LovelyMarburg🏰❤️
The best?🤔The people❤️
Endless gratitude to Tobi & Anke - amazing role models❤️ Love the "thanks BBQ no matter what" spirit from the "Präsidium"! @unimarburg.bsky.social - sure it tasted better as we got it😉😂 #ProfLife #LovelyMarburg🏰❤️
May 24, 2025 at 9:00 AM
Marburg rules. I see @rebeleinlab.bsky.social @schullerjm.bsky.social @georghochberg.bsky.social in the crowd 😘
Reposted by Johannes Rebelein
Out now in @chemicalscience.rsc.org:
We apply 2DIR spectroscopy to study [FeFe] hydrogenase for the first time. Check it out!
pubs.rsc.org/en/content/a...
We apply 2DIR spectroscopy to study [FeFe] hydrogenase for the first time. Check it out!
pubs.rsc.org/en/content/a...
Two-dimensional Infrared Spectroscopy as a Tool to Reveal the Vibrational and Molecular Structure of [FeFe] Hydrogenases
[FeFe] hydrogenases are Nature’s most efficient catalysts for the cleavage and evolution of molecular hydrogen. Despite decades of research, key aspects of the catalytic cycle and the underlying geome...
pubs.rsc.org
May 10, 2025 at 1:35 PM
Out now in @chemicalscience.rsc.org:
We apply 2DIR spectroscopy to study [FeFe] hydrogenase for the first time. Check it out!
pubs.rsc.org/en/content/a...
We apply 2DIR spectroscopy to study [FeFe] hydrogenase for the first time. Check it out!
pubs.rsc.org/en/content/a...
Reposted by Johannes Rebelein
The National Climate Assessment — already in the works for months — was gutted earlier this week, when President Trump’s administration dismissed hundreds of scientists working on it..
The National Nature Assessment was cancelled on his first day back in office..
🧪🦠
www.nature.com/articles/d41...
The National Nature Assessment was cancelled on his first day back in office..
🧪🦠
www.nature.com/articles/d41...
Trump gutted two landmark environmental reports — can researchers save them?
Scientists working on disrupted national climate and nature assessments aren’t giving up.
www.nature.com
May 4, 2025 at 3:33 AM
The National Climate Assessment — already in the works for months — was gutted earlier this week, when President Trump’s administration dismissed hundreds of scientists working on it..
The National Nature Assessment was cancelled on his first day back in office..
🧪🦠
www.nature.com/articles/d41...
The National Nature Assessment was cancelled on his first day back in office..
🧪🦠
www.nature.com/articles/d41...
Reposted by Johannes Rebelein
I think a lot about what Carl Sagan said in one of his final interviews.
May 4, 2025 at 6:21 AM
I think a lot about what Carl Sagan said in one of his final interviews.
Reposted by Johannes Rebelein
*** Only a few days left to apply ***
Postdoc position at the University of Marburg (Germany), funded within our ERC Advanced Grant "C-SWITCH"
Spatiotemporal regulation of cellular processes by CTP-switches in bacteria
Apply by 04 May: stellenangebote.uni-marburg.de/jobposting/2...
Postdoc position at the University of Marburg (Germany), funded within our ERC Advanced Grant "C-SWITCH"
Spatiotemporal regulation of cellular processes by CTP-switches in bacteria
Apply by 04 May: stellenangebote.uni-marburg.de/jobposting/2...
May 1, 2025 at 11:17 AM
*** Only a few days left to apply ***
Postdoc position at the University of Marburg (Germany), funded within our ERC Advanced Grant "C-SWITCH"
Spatiotemporal regulation of cellular processes by CTP-switches in bacteria
Apply by 04 May: stellenangebote.uni-marburg.de/jobposting/2...
Postdoc position at the University of Marburg (Germany), funded within our ERC Advanced Grant "C-SWITCH"
Spatiotemporal regulation of cellular processes by CTP-switches in bacteria
Apply by 04 May: stellenangebote.uni-marburg.de/jobposting/2...
Reposted by Johannes Rebelein
My dept in London at Imperial (Bioengineering) is recruiting a group leader at Professor level 🧑🎓 Please RT. It's a great place with world-class expertise in all sorts of biomedical & biological engineering topics like synthetic biology. It's also not in the USA. www.imperial.ac.uk/jobs/search-...
May 2, 2025 at 3:20 PM
My dept in London at Imperial (Bioengineering) is recruiting a group leader at Professor level 🧑🎓 Please RT. It's a great place with world-class expertise in all sorts of biomedical & biological engineering topics like synthetic biology. It's also not in the USA. www.imperial.ac.uk/jobs/search-...
New preprint from @erdrich-science.bsky.social of our collaborative project with @borjana-a.bsky.social, @fz-juelich.de and @guidogrossmann.bsky.social @hhu.de:
"Phage biocontrol reduces the burden on plant immunity through suppression of bacterial virulence"
www.biorxiv.org/content/10.1...
"Phage biocontrol reduces the burden on plant immunity through suppression of bacterial virulence"
www.biorxiv.org/content/10.1...
Phage biocontrol reduces the burden on plant immunity through suppression of bacterial virulence
Bacteriophages are increasingly recognized as key players in modulating plant-microbe interactions, including their potential in the biocontrol of plant pathogenic bacteria. In this study, we investig...
www.biorxiv.org
May 3, 2025 at 7:39 PM
Reposted by Johannes Rebelein
New preprint from @erdrich-science.bsky.social of our collaborative project with @borjana-a.bsky.social, @fz-juelich.de and @guidogrossmann.bsky.social @hhu.de:
"Phage biocontrol reduces the burden on plant immunity through suppression of bacterial virulence"
www.biorxiv.org/content/10.1...
"Phage biocontrol reduces the burden on plant immunity through suppression of bacterial virulence"
www.biorxiv.org/content/10.1...
Phage biocontrol reduces the burden on plant immunity through suppression of bacterial virulence
Bacteriophages are increasingly recognized as key players in modulating plant-microbe interactions, including their potential in the biocontrol of plant pathogenic bacteria. In this study, we investig...
www.biorxiv.org
May 3, 2025 at 1:58 PM
New preprint from @erdrich-science.bsky.social of our collaborative project with @borjana-a.bsky.social, @fz-juelich.de and @guidogrossmann.bsky.social @hhu.de:
"Phage biocontrol reduces the burden on plant immunity through suppression of bacterial virulence"
www.biorxiv.org/content/10.1...
"Phage biocontrol reduces the burden on plant immunity through suppression of bacterial virulence"
www.biorxiv.org/content/10.1...
Exciting work by Ribbe & Hu lab demonstrating the in vivo reduction of N2 by L-cluster containing Nif(EN)2 inside E. coli: www.science.org/doi/10.1126/... This finding suggests a smaller minimal gene set for the heterologous expression of #nitrogenase and an easier route to synthetic #N2-fixation
Heterologous synthesis of a simplified nitrogenase analog in Escherichia coli
Heterologous synthesis of a nitrogenase analog (NifH/NifEN) in E. coli enables N2 reduction and incorporation of N into biomass.
www.science.org
May 3, 2025 at 7:35 PM
Exciting work by Ribbe & Hu lab demonstrating the in vivo reduction of N2 by L-cluster containing Nif(EN)2 inside E. coli: www.science.org/doi/10.1126/... This finding suggests a smaller minimal gene set for the heterologous expression of #nitrogenase and an easier route to synthetic #N2-fixation
Great work Adrian!
🚨Out now in Angewandte Chemie:
AI.zymes - A modular platform for evolutionary enzyme design.
AI.zymes integrates established and emerging bioengineering tools into a coherent pipeline for #enzymedesign.
📄Paper: doi.org/10.1002/anie...
💻GitHub: github.com/bunzela/AIzy...
AI.zymes - A modular platform for evolutionary enzyme design.
AI.zymes integrates established and emerging bioengineering tools into a coherent pipeline for #enzymedesign.
📄Paper: doi.org/10.1002/anie...
💻GitHub: github.com/bunzela/AIzy...
May 3, 2025 at 3:32 PM
Great work Adrian!
Reposted by Johannes Rebelein
Enabling next-generation anaerobic cultivation through biotechnology to advance functional microbiome research www.nature.com/articles/s41... #jcampubs
Enabling next-generation anaerobic cultivation through biotechnology to advance functional microbiome research - Nature Biotechnology
Overcoming key hurdles for the development of next-generation anaerobic cultivation methods could have major impacts on microbial community research and applications.
www.nature.com
April 29, 2025 at 5:06 PM
Enabling next-generation anaerobic cultivation through biotechnology to advance functional microbiome research www.nature.com/articles/s41... #jcampubs
Reposted by Johannes Rebelein
Gab es dazu wirklich KEINE Berichterstattung? Eine Google-News Suche zu CO2-Konzentration liefert nur Ergebnisse zu einem UN-Bericht aus 2024. Keine Studie, keine News? Außer natürlich bei BIP, Inflation, Arbeitslosigkeit... 😬 Bitte gerne Links darunter posten.
Wahnsinn. Dieser nie dagewesene Anstieg der CO2-Konzentration in einem Jahr ist jenseits aller Erwartungen und müsste in zig Sondersendungen erörtert werden, wenn wir als Gesellschaft noch bei Trost wären. Müsste... gml.noaa.gov/ccgg/trends/...
April 20, 2025 at 5:35 AM
Gab es dazu wirklich KEINE Berichterstattung? Eine Google-News Suche zu CO2-Konzentration liefert nur Ergebnisse zu einem UN-Bericht aus 2024. Keine Studie, keine News? Außer natürlich bei BIP, Inflation, Arbeitslosigkeit... 😬 Bitte gerne Links darunter posten.
Reposted by Johannes Rebelein
Energy from air? No problem! In our new preprint, we reconstitute the machinery that allows microbes to endure starvation. By using the trace amounts of hydrogen in air alone, they produce plenty of chemical energy (2 ATP) to get by. The only byproduct? Water. www.biorxiv.org/content/10.1...
Hydrogenase-driven ATP synthesis from air
All cells require a continuous supply of the universal energy currency, adenosine triphosphate (ATP), to drive countless cellular reactions. The universally conserved F1Fo-ATP synthase regenerates ATP...
www.biorxiv.org
March 20, 2025 at 12:57 AM
Energy from air? No problem! In our new preprint, we reconstitute the machinery that allows microbes to endure starvation. By using the trace amounts of hydrogen in air alone, they produce plenty of chemical energy (2 ATP) to get by. The only byproduct? Water. www.biorxiv.org/content/10.1...
Reposted by Johannes Rebelein
Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍
Preprint📜: www.biorxiv.org/content/10.1...
A short thread🧵👇
Preprint📜: www.biorxiv.org/content/10.1...
A short thread🧵👇
January 6, 2025 at 11:19 AM
Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍
Preprint📜: www.biorxiv.org/content/10.1...
A short thread🧵👇
Preprint📜: www.biorxiv.org/content/10.1...
A short thread🧵👇
Reposted by Johannes Rebelein
One of the largest open questions in anaerobic metabolism solved. The best work of my lab yet, performed by the incredible hard working, talented and motivated coworkers: @fidelormz.bsky.social , @sophia-pa.bsky.social and @rnfr2d2.bsky.social. I am a proud PI today!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structure of the ATP-driven methyl-coenzyme M reductase activation complex - Nature
The structure and function of the MCR activation complex from Methanococcus maripaludis were revealed, demonstrating its ATP-dependent ability to activate MCR and form methane while uncovering a uniqu...
www.nature.com
April 16, 2025 at 4:09 PM
One of the largest open questions in anaerobic metabolism solved. The best work of my lab yet, performed by the incredible hard working, talented and motivated coworkers: @fidelormz.bsky.social , @sophia-pa.bsky.social and @rnfr2d2.bsky.social. I am a proud PI today!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
This is an excellent explanation about the implications of finding #nitrogenase #metalloclusters in the MCR activation complex
This is a wild paper. The anaerobic biochemistry is definitely worth your while (and beyond me to explain), but let me try to explain why this is also an evolutionary bombshell. This is about how nitrogen fixation evolved as we know it.
I'm truly excited to announce our new publication in @nature.com unraveling a central picture of the Methyl-coenzyme M reductase (MCR) activation machinery and it's strong ATP dependency - kudos to @rnfr2d2.bsky.social for the fantastic illustration!
LINK: www.nature.com/articles/s41...
LINK: www.nature.com/articles/s41...
April 19, 2025 at 7:24 PM
This is an excellent explanation about the implications of finding #nitrogenase #metalloclusters in the MCR activation complex
Reposted by Johannes Rebelein
I'm truly excited to announce our new publication in @nature.com unraveling a central picture of the Methyl-coenzyme M reductase (MCR) activation machinery and it's strong ATP dependency - kudos to @rnfr2d2.bsky.social for the fantastic illustration!
LINK: www.nature.com/articles/s41...
LINK: www.nature.com/articles/s41...
April 16, 2025 at 4:20 PM
I'm truly excited to announce our new publication in @nature.com unraveling a central picture of the Methyl-coenzyme M reductase (MCR) activation machinery and it's strong ATP dependency - kudos to @rnfr2d2.bsky.social for the fantastic illustration!
LINK: www.nature.com/articles/s41...
LINK: www.nature.com/articles/s41...