Rhett Rautsaw
@reptilerhett.bsky.social
All views are my own
Field Applications Bioinformatic Support (FABS) Scientist @pacbio.bsky.social
PostDoc: Tasmanian Devils @wsupullman.bsky.social & @usouthflorida.bsky.social
PhD: 🐍 venom evolution @SnakeLabClemson
Field Applications Bioinformatic Support (FABS) Scientist @pacbio.bsky.social
PostDoc: Tasmanian Devils @wsupullman.bsky.social & @usouthflorida.bsky.social
PhD: 🐍 venom evolution @SnakeLabClemson
Reposted by Rhett Rautsaw
SPRQ-Nx is coming to #Revio next year. It is expected to reduce whole genome sequencing costs by up to 40% to $300–$350 per genome or lower at scale.
#PacBio #ASHG25 #HiFisequencing #ASHG
#PacBio #ASHG25 #HiFisequencing #ASHG
October 17, 2025 at 4:36 PM
SPRQ-Nx is coming to #Revio next year. It is expected to reduce whole genome sequencing costs by up to 40% to $300–$350 per genome or lower at scale.
#PacBio #ASHG25 #HiFisequencing #ASHG
#PacBio #ASHG25 #HiFisequencing #ASHG
Reposted by Rhett Rautsaw
The #PacBioBlockParty is in full swing 🎉 Guests are rolling in, the music’s on, and the fun’s just getting started! Virtual darts, bowling, food, a live DJ, and more await. Let’s celebrate together!
#ASHG25 #PacBio #ASHG
#ASHG25 #PacBio #ASHG
October 16, 2025 at 1:26 AM
The #PacBioBlockParty is in full swing 🎉 Guests are rolling in, the music’s on, and the fun’s just getting started! Virtual darts, bowling, food, a live DJ, and more await. Let’s celebrate together!
#ASHG25 #PacBio #ASHG
#ASHG25 #PacBio #ASHG
Reposted by Rhett Rautsaw
Short reads…who needs em? Start bringing ALL your NGS projects into the long read era with cheaper sequencing, simultaneous methylation, greater insights, and more answers to your biological questions. PacBio’s got you covered.
October 15, 2025 at 12:27 AM
Short reads…who needs em? Start bringing ALL your NGS projects into the long read era with cheaper sequencing, simultaneous methylation, greater insights, and more answers to your biological questions. PacBio’s got you covered.
Reposted by Rhett Rautsaw
Just in: #PacBio HiFi long-read sequencing is powering the National Institute on Aging’s Long Life Family Study.
Up to 7,800 genomes and epigenomes will be sequenced on Revio systems to uncover rare variants, methylation patterns, and other factors linked to healthy aging.
🔗 bit.ly/3IUDdFo
Up to 7,800 genomes and epigenomes will be sequenced on Revio systems to uncover rare variants, methylation patterns, and other factors linked to healthy aging.
🔗 bit.ly/3IUDdFo
October 8, 2025 at 3:02 PM
Just in: #PacBio HiFi long-read sequencing is powering the National Institute on Aging’s Long Life Family Study.
Up to 7,800 genomes and epigenomes will be sequenced on Revio systems to uncover rare variants, methylation patterns, and other factors linked to healthy aging.
🔗 bit.ly/3IUDdFo
Up to 7,800 genomes and epigenomes will be sequenced on Revio systems to uncover rare variants, methylation patterns, and other factors linked to healthy aging.
🔗 bit.ly/3IUDdFo
Reposted by Rhett Rautsaw
I'm heading to Boston for #ASHG25 tomorrow, let me know if you'd like to chat about using HiFi sequencing for mitochondrial analyses, pharmacogenomics, or anything else!
ICYMI, also happy to discuss our new Aardvark tool for benchmarking :)
ICYMI, also happy to discuss our new Aardvark tool for benchmarking :)
I'm excited to share our pre-print about a new variant benchmarking tool we've been working on for the past few months!
Aardvark: Sifting through differences in a mound of variants
GitHub: github.com/PacificBiosc...
Some highlights in this thread:
1/N
Aardvark: Sifting through differences in a mound of variants
GitHub: github.com/PacificBiosc...
Some highlights in this thread:
1/N
October 13, 2025 at 1:48 PM
I'm heading to Boston for #ASHG25 tomorrow, let me know if you'd like to chat about using HiFi sequencing for mitochondrial analyses, pharmacogenomics, or anything else!
ICYMI, also happy to discuss our new Aardvark tool for benchmarking :)
ICYMI, also happy to discuss our new Aardvark tool for benchmarking :)
Reposted by Rhett Rautsaw
We just released a major update for StarPhase that adds support for inexact matching PGx alleles. We're hoping this streamlines the process for discovering novel allele combinations with #PacBio #HiFi data, feedback welcome!
Full notes here: github.com/PacificBiosc...
#ASHG25
Full notes here: github.com/PacificBiosc...
#ASHG25
Release StarPhase v1.5.0 · PacificBiosciences/pb-StarPhase
Changes
Adds a new field to the output json, inexact_diplotypes. This field is intended to capture information about inexact allele matches. The behavior of this output depends on the gene:
For s...
github.com
October 13, 2025 at 8:13 PM
We just released a major update for StarPhase that adds support for inexact matching PGx alleles. We're hoping this streamlines the process for discovering novel allele combinations with #PacBio #HiFi data, feedback welcome!
Full notes here: github.com/PacificBiosc...
#ASHG25
Full notes here: github.com/PacificBiosc...
#ASHG25
Reposted by Rhett Rautsaw
Updated tutorials for nQuack today. Want to predict ploidy level of sequence data? See this new tutorial on how to process and model data faster: mlgaynor.com/nQuack/artic...
a little boy is driving a red toy car on the street .
Alt: a little kid is driving a red toy car on the street.
media.tenor.com
October 14, 2025 at 1:32 AM
Updated tutorials for nQuack today. Want to predict ploidy level of sequence data? See this new tutorial on how to process and model data faster: mlgaynor.com/nQuack/artic...
Reposted by Rhett Rautsaw
In a landmark #PopulationGenomics study, All of Us researchers used #HiFisequencing of 1,027 participants to identify hundreds of #StructuralVariants disease associations, with over half missed by short reads. What are you missing in your research?
Read the study here: bit.ly/4ocq49K
#PacBio
Read the study here: bit.ly/4ocq49K
#PacBio
Population-scale Long-read Sequencing in the All of Us Research Program
The All of Us Research Program (AoU) is a national biobank seeking to enroll one million individuals in the United States to link genomic and biomedical data, including short- and long-read whole-geno...
bit.ly
October 7, 2025 at 7:00 PM
In a landmark #PopulationGenomics study, All of Us researchers used #HiFisequencing of 1,027 participants to identify hundreds of #StructuralVariants disease associations, with over half missed by short reads. What are you missing in your research?
Read the study here: bit.ly/4ocq49K
#PacBio
Read the study here: bit.ly/4ocq49K
#PacBio
Reposted by Rhett Rautsaw
Now published in Cell! We found that ~15% of SNPs from divergent refs did not liftover as SNPs in the gray fox ref—half mapped to monomorphic sites, half failed to map. Co-authored with Matthew Genchev, @elliecat.bsky.social, and @jazlynmooney.bsky.social
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
September 22, 2025 at 5:41 PM
Now published in Cell! We found that ~15% of SNPs from divergent refs did not liftover as SNPs in the gray fox ref—half mapped to monomorphic sites, half failed to map. Co-authored with Matthew Genchev, @elliecat.bsky.social, and @jazlynmooney.bsky.social
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Reposted by Rhett Rautsaw
It absolutely warms my heart when I click on a pre-print that includes a bunch of top methods folks from industry and I see (1) open-source software (with a good license; MIT in this case) that is (2) written in Rust. Bravo @pacbio.bsky.social!
Moar Rust plz!
github.com/PacificBiosc...
Moar Rust plz!
github.com/PacificBiosc...
October 6, 2025 at 7:57 PM
It absolutely warms my heart when I click on a pre-print that includes a bunch of top methods folks from industry and I see (1) open-source software (with a good license; MIT in this case) that is (2) written in Rust. Bravo @pacbio.bsky.social!
Moar Rust plz!
github.com/PacificBiosc...
Moar Rust plz!
github.com/PacificBiosc...
Reposted by Rhett Rautsaw
I'm excited to share our pre-print about a new variant benchmarking tool we've been working on for the past few months!
Aardvark: Sifting through differences in a mound of variants
GitHub: github.com/PacificBiosc...
Some highlights in this thread:
1/N
Aardvark: Sifting through differences in a mound of variants
GitHub: github.com/PacificBiosc...
Some highlights in this thread:
1/N
October 6, 2025 at 8:07 PM
I'm excited to share our pre-print about a new variant benchmarking tool we've been working on for the past few months!
Aardvark: Sifting through differences in a mound of variants
GitHub: github.com/PacificBiosc...
Some highlights in this thread:
1/N
Aardvark: Sifting through differences in a mound of variants
GitHub: github.com/PacificBiosc...
Some highlights in this thread:
1/N
Reposted by Rhett Rautsaw
The 2025 #Epigenetics SMRT Grant is open!
Submit a 100-word proposal for a chance to win free sequencing with Fiber-seq, which combines genome sequence, 5mC methylation, and chromatin accessibility for a multiomic view of regulation.
Apply here: bit.ly/4nGQrEW
Submit a 100-word proposal for a chance to win free sequencing with Fiber-seq, which combines genome sequence, 5mC methylation, and chromatin accessibility for a multiomic view of regulation.
Apply here: bit.ly/4nGQrEW
October 6, 2025 at 4:55 PM
The 2025 #Epigenetics SMRT Grant is open!
Submit a 100-word proposal for a chance to win free sequencing with Fiber-seq, which combines genome sequence, 5mC methylation, and chromatin accessibility for a multiomic view of regulation.
Apply here: bit.ly/4nGQrEW
Submit a 100-word proposal for a chance to win free sequencing with Fiber-seq, which combines genome sequence, 5mC methylation, and chromatin accessibility for a multiomic view of regulation.
Apply here: bit.ly/4nGQrEW
Reposted by Rhett Rautsaw
Today marks the first day in public media’s history without federal funding. And we’re not going anywhere.
Listeners like you keep our mission alive. Protect one of the last places where America comes together to hear itself.
Stand with us today. Donate at this link: n.pr/46wamAj
Listeners like you keep our mission alive. Protect one of the last places where America comes together to hear itself.
Stand with us today. Donate at this link: n.pr/46wamAj
October 1, 2025 at 2:19 PM
Today marks the first day in public media’s history without federal funding. And we’re not going anywhere.
Listeners like you keep our mission alive. Protect one of the last places where America comes together to hear itself.
Stand with us today. Donate at this link: n.pr/46wamAj
Listeners like you keep our mission alive. Protect one of the last places where America comes together to hear itself.
Stand with us today. Donate at this link: n.pr/46wamAj
Reposted by Rhett Rautsaw
Did you know? Revio & Vega include on-instrument 6mA calling for the Fiber-seq chromatin accessibility assay. Thanks to Fiber-seq expert @mrvollger.bsky.social for the thoughtful evaluation and recommendation.
Reagents for the assay are also available from our friends at EpiCypher: bit.ly/42YwGQE
Reagents for the assay are also available from our friends at EpiCypher: bit.ly/42YwGQE
October 2, 2025 at 5:33 PM
Did you know? Revio & Vega include on-instrument 6mA calling for the Fiber-seq chromatin accessibility assay. Thanks to Fiber-seq expert @mrvollger.bsky.social for the thoughtful evaluation and recommendation.
Reagents for the assay are also available from our friends at EpiCypher: bit.ly/42YwGQE
Reagents for the assay are also available from our friends at EpiCypher: bit.ly/42YwGQE
Reposted by Rhett Rautsaw
Can one test replace many?
A JAMA Pediatrics study by @childrensmercy.org evaluated long-read HiFi sequencing as a first-line approach for pediatric rare disease research, with potential to improve solves, reduce turnaround time, and bypass multiple sequential testing.
Read more: bit.ly/3VtHjHh
A JAMA Pediatrics study by @childrensmercy.org evaluated long-read HiFi sequencing as a first-line approach for pediatric rare disease research, with potential to improve solves, reduce turnaround time, and bypass multiple sequential testing.
Read more: bit.ly/3VtHjHh
Our new study in Journal of the American Medical Association Pediatrics shows a single long-read sequencing test can ID rare genetic conditions in kids faster & more accurately. This means fewer tests, quicker answers & a potential breakthrough.
@research.childrensmercy.org
https://cmkc.link/4gFemlo
@research.childrensmercy.org
https://cmkc.link/4gFemlo
Unlocking Answers Faster: Children’s Mercy Revolutionizes Genetic Disease Diagnosis with a Single-Test
Children’s Mercy, one of the nation’s top independent pediatric health systems, is once again leading the way in genomic medicine. In a landmark study, published in the prestigious Journal of the Amer...
cmkc.link
September 25, 2025 at 3:58 PM
Can one test replace many?
A JAMA Pediatrics study by @childrensmercy.org evaluated long-read HiFi sequencing as a first-line approach for pediatric rare disease research, with potential to improve solves, reduce turnaround time, and bypass multiple sequential testing.
Read more: bit.ly/3VtHjHh
A JAMA Pediatrics study by @childrensmercy.org evaluated long-read HiFi sequencing as a first-line approach for pediatric rare disease research, with potential to improve solves, reduce turnaround time, and bypass multiple sequential testing.
Read more: bit.ly/3VtHjHh
Reposted by Rhett Rautsaw
🚨 Last call! This week, join PAG’s free webinar to see how long-read low-pass sequencing and pangenomes are reshaping #genomics.
From structural variation to trait discovery, explore scalable insights across species. Sponsored by #PacBio.
Register: bit.ly/4mbCkFU
From structural variation to trait discovery, explore scalable insights across species. Sponsored by #PacBio.
Register: bit.ly/4mbCkFU
September 22, 2025 at 10:10 PM
🚨 Last call! This week, join PAG’s free webinar to see how long-read low-pass sequencing and pangenomes are reshaping #genomics.
From structural variation to trait discovery, explore scalable insights across species. Sponsored by #PacBio.
Register: bit.ly/4mbCkFU
From structural variation to trait discovery, explore scalable insights across species. Sponsored by #PacBio.
Register: bit.ly/4mbCkFU
Reposted by Rhett Rautsaw
The 2025 Long-Read Low Pass SMRT Grant for plant + animal sciences is now open! 🌱🐾
Apply to win free PacBio HiFi sequencing for your conservation or agricultural research.
Submit your 100-word proposal today: bit.ly/3IgrReu
#PacBio #SMRTGrant #HiFisequencing
Apply to win free PacBio HiFi sequencing for your conservation or agricultural research.
Submit your 100-word proposal today: bit.ly/3IgrReu
#PacBio #SMRTGrant #HiFisequencing
September 15, 2025 at 8:11 PM
The 2025 Long-Read Low Pass SMRT Grant for plant + animal sciences is now open! 🌱🐾
Apply to win free PacBio HiFi sequencing for your conservation or agricultural research.
Submit your 100-word proposal today: bit.ly/3IgrReu
#PacBio #SMRTGrant #HiFisequencing
Apply to win free PacBio HiFi sequencing for your conservation or agricultural research.
Submit your 100-word proposal today: bit.ly/3IgrReu
#PacBio #SMRTGrant #HiFisequencing
Reposted by Rhett Rautsaw
Ready to celebrate #ASHG25? 🎉
Join us at the PacBio Block Party on October 15 in Boston to network with fellow scientists, connect with the genomics community, and honor 25 years of discoveries and innovation.
RSVP now: bit.ly/46xnUeI
#PacBioBlockParty
Join us at the PacBio Block Party on October 15 in Boston to network with fellow scientists, connect with the genomics community, and honor 25 years of discoveries and innovation.
RSVP now: bit.ly/46xnUeI
#PacBioBlockParty
September 15, 2025 at 3:42 PM
Ready to celebrate #ASHG25? 🎉
Join us at the PacBio Block Party on October 15 in Boston to network with fellow scientists, connect with the genomics community, and honor 25 years of discoveries and innovation.
RSVP now: bit.ly/46xnUeI
#PacBioBlockParty
Join us at the PacBio Block Party on October 15 in Boston to network with fellow scientists, connect with the genomics community, and honor 25 years of discoveries and innovation.
RSVP now: bit.ly/46xnUeI
#PacBioBlockParty
Reposted by Rhett Rautsaw
New Editor's Choice article:
"Island biogeography and competition drive rapid venom complexity evolution across rattlesnakes" by Hirst et al.
doi.org/10.1093/evol...
@hirsticane.bsky.social @RamsesRosales11.bsky.social @marcabeer.bsky.social @jason-strickland.bsky.social @reptilerhett.bsky.social
"Island biogeography and competition drive rapid venom complexity evolution across rattlesnakes" by Hirst et al.
doi.org/10.1093/evol...
@hirsticane.bsky.social @RamsesRosales11.bsky.social @marcabeer.bsky.social @jason-strickland.bsky.social @reptilerhett.bsky.social
September 15, 2025 at 10:36 AM
New Editor's Choice article:
"Island biogeography and competition drive rapid venom complexity evolution across rattlesnakes" by Hirst et al.
doi.org/10.1093/evol...
@hirsticane.bsky.social @RamsesRosales11.bsky.social @marcabeer.bsky.social @jason-strickland.bsky.social @reptilerhett.bsky.social
"Island biogeography and competition drive rapid venom complexity evolution across rattlesnakes" by Hirst et al.
doi.org/10.1093/evol...
@hirsticane.bsky.social @RamsesRosales11.bsky.social @marcabeer.bsky.social @jason-strickland.bsky.social @reptilerhett.bsky.social
September 13, 2025 at 10:22 PM
Reposted by Rhett Rautsaw
From low-pass seq that scales discovery to pangenomes that reveal hidden variation, long-read technologies are transforming genomics.
Join PAG’s free webinar to explore insights reshaping research across the tree of life. Sponsored by #PacBio
Register: bit.ly/4mbCkFU
Join PAG’s free webinar to explore insights reshaping research across the tree of life. Sponsored by #PacBio
Register: bit.ly/4mbCkFU
September 11, 2025 at 7:42 PM
From low-pass seq that scales discovery to pangenomes that reveal hidden variation, long-read technologies are transforming genomics.
Join PAG’s free webinar to explore insights reshaping research across the tree of life. Sponsored by #PacBio
Register: bit.ly/4mbCkFU
Join PAG’s free webinar to explore insights reshaping research across the tree of life. Sponsored by #PacBio
Register: bit.ly/4mbCkFU
Reposted by Rhett Rautsaw
Always excited for an increased salary submission! Congrats to the Department of Biological Sciences at New Jersey Institute of Tech for their stipend increase! This union-based win brings the pay almost to the 1:1 line! Update your department and find out more here: rhettrautsaw.app/shiny/Biolog...
September 10, 2025 at 2:48 PM
Always excited for an increased salary submission! Congrats to the Department of Biological Sciences at New Jersey Institute of Tech for their stipend increase! This union-based win brings the pay almost to the 1:1 line! Update your department and find out more here: rhettrautsaw.app/shiny/Biolog...
Reposted by Rhett Rautsaw
Most RNA-seq methods feel like stir-fry. Transcripts diced into bits, then tossed back together.
HiFi long reads bring the whole roast. Full transcripts served intact, end to end.
Need to see how? Get the Seq 101 guide & a chance at a sponsored conference: bit.ly/3JminPa
#TheresHiFiForThat
HiFi long reads bring the whole roast. Full transcripts served intact, end to end.
Need to see how? Get the Seq 101 guide & a chance at a sponsored conference: bit.ly/3JminPa
#TheresHiFiForThat
September 9, 2025 at 10:38 PM
Most RNA-seq methods feel like stir-fry. Transcripts diced into bits, then tossed back together.
HiFi long reads bring the whole roast. Full transcripts served intact, end to end.
Need to see how? Get the Seq 101 guide & a chance at a sponsored conference: bit.ly/3JminPa
#TheresHiFiForThat
HiFi long reads bring the whole roast. Full transcripts served intact, end to end.
Need to see how? Get the Seq 101 guide & a chance at a sponsored conference: bit.ly/3JminPa
#TheresHiFiForThat
Reposted by Rhett Rautsaw
Looking to adopt long-read sequencing in your lab?
The #PacBio Pre-Clinical Playbook has best practices, workflow guidance, case studies, and real-world insights to help you implement #HiFisequencing with confidence.
🔗 Download now: bit.ly/4mpZ85b
The #PacBio Pre-Clinical Playbook has best practices, workflow guidance, case studies, and real-world insights to help you implement #HiFisequencing with confidence.
🔗 Download now: bit.ly/4mpZ85b
September 8, 2025 at 4:17 PM
Looking to adopt long-read sequencing in your lab?
The #PacBio Pre-Clinical Playbook has best practices, workflow guidance, case studies, and real-world insights to help you implement #HiFisequencing with confidence.
🔗 Download now: bit.ly/4mpZ85b
The #PacBio Pre-Clinical Playbook has best practices, workflow guidance, case studies, and real-world insights to help you implement #HiFisequencing with confidence.
🔗 Download now: bit.ly/4mpZ85b