Mart Krupovic
banner
mkrupovic.bsky.social
Mart Krupovic
@mkrupovic.bsky.social
Virus origins/diversity/evolution; mobilome; Archaea.
Head of the Cell Biology and Virology of Archaea Unit at Institut Pasteur:
https://research.pasteur.fr/en/team/archaeal-virology/
Reposted by Mart Krupovic
Preprint: Bacteria sense virus-induced genome degradation via methylated mononucleotides

tinyurl.com/ch3damp

We show how molecular byproducts released during virus-induced cell exploitation are used as signals to trigger host immunity

Revealed by the amazing Ilya Osterman. See his thread below👇
November 6, 2025 at 10:39 AM
Reposted by Mart Krupovic
Check out the fancy anti-parallel cytochrome nanowire bundles structure we've solved. This structure originates from a bacterium, strain name WTL (well-to-live), isolated by the Bond group from the Soudan Underground Iron Mine, located 713 m below the surface. www.biorxiv.org/content/10.1...
A Bundled Antiparallel Cytochrome Nanowire Structure Suggests Roles in Cell-Cell Electron Transfer and Biofilm Formation
Long-range extracellular electron transfer enables respiring microbes to use minerals, other organisms, or electrodes as electron acceptors by transporting electrons microns away from the cell surface...
www.biorxiv.org
November 3, 2025 at 3:48 PM
Reposted by Mart Krupovic
Curious about the top methane-maker in your gut? Meet this month's featured microbe and find out! 🦠💨🔥 in @cp-trendsmicrobiol.bsky.social #archaeasky
@sgribaldo.bsky.social @pasteur.fr
🔗 Full article linked in reply👇
www.sciencedirect.com/science/arti...
Methanobrevibacter smithii
www.sciencedirect.com
November 3, 2025 at 2:58 PM
Reposted by Mart Krupovic
This paper has been a must! Great collaboration with @mkrupovic.bsky.social and @yifanzhou.bsky.social, a N&V by a legend of halophilic archaea tinyurl.com/yc3dcv72, and one picture of one of our expeditions to Dallol making the cover of the November issue of @natmicrobiol.nature.com

rdcu.be/eLtCH
November 2, 2025 at 5:52 PM
Reposted by Mart Krupovic
Applications closing tonight (midnight French time) !!!
Still a few hours to apply !!
Open position to work on Type IX secretion (#T9SS) in our lab, in collaboration withe the group of Eric Reynolds at the Dental School of the University of Melbourne. Please spread the word, and forward to anyone potentially interested ! Apply here:
emploi.cnrs.fr/Offres/Docto...
Portail Emploi CNRS - Offre d'emploi - Thèse en Microbiologie-Biochimie (H/F)
emploi.cnrs.fr
October 30, 2025 at 1:17 PM
Reposted by Mart Krupovic
📣 PhD Scholarship Opportunity for International Students!
Are you looking to do a PhD in soil microbial and viral ecology?
✍️ Get in touch now!!
✅ Requirements: Masters degree, Distinguished CV, Highly motivated
⏳ Application deadline: November 24
www.univ-lyon1.fr/recherche/fo...
APPEL A PROJET
www.univ-lyon1.fr
October 21, 2025 at 5:36 PM
Reposted by Mart Krupovic
Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...
October 28, 2025 at 3:07 PM
Reposted by Mart Krupovic
First time posting on Bluesky! 🥳 We discovered a new pilus family in cyanobacteria. The pilin has a novel protein fold that forms a super-tight double-layer tube. Similar gene clusters exist in a few cyanobacterial orders. A great collaboration with John and others! www.nature.com/articles/s41...
A family of tubular pili from harmful algal bloom forming cyanobacterium Microcystis aeruginosa - Nature Communications
Ricca et al discover a new family of tubular pili in Microcystis aeruginosa, a harmful algal bloom-forming cyanobacterium. These pili are crucial for buoyancy by forming cell micro-colonies, which inc...
www.nature.com
October 24, 2025 at 4:08 AM
Reposted by Mart Krupovic
Glad to share our paper out today @NatureEcoEvo: “Serial innovations by Asgard archaea shaped the DNA replication machinery of the early eukaryotic ancestor”. www.nature.com/articles/s41... #microsky #archaeasky
Serial innovations by Asgard archaea shaped the DNA replication machinery of the early eukaryotic ancestor - Nature Ecology & Evolution
Phylogenetic and biochemical analyses show a diversity of components of the DNA replication machinery in different Asgard archaea that contributed to the eukaryotic DNA replication machinery.
www.nature.com
October 21, 2025 at 3:06 PM
Reposted by Mart Krupovic
Viruses and virus satellites of haloarchaea and their nanosized DPANN symbionts reveal intricate nested interactions

Out now in Nature Microbiology, by Yifan Zhou, Mart Krupovic & colleagues.
@mkrupovic.bsky.social
@yifanzhou.bsky.social
@deemteam.bsky.social

www.nature.com/articles/s41...
Viruses and virus satellites of haloarchaea and their nanosized DPANN symbionts reveal intricate nested interactions - Nature Microbiology
An exploration of the viromes of haloarchaea and their ultra-small DPANN symbionts reveals plasmid-derived satellites of viruses from both archaeal groups, highlighting the complexity of nested symbio...
www.nature.com
October 20, 2025 at 7:46 PM
Reposted by Mart Krupovic
Now published: EnVhogDB, a large database of viral protein family HMM profiles to help functional annotation of new phage genomes - peercommunityjournal.org/articles/10....
EnVhogDB: an extended view of the viral protein families on Earth through a vast collection of HMM profiles
peercommunityjournal.org
October 18, 2025 at 7:58 AM
Check out our paper on intricate nested interactions between viruses and virus satellites of haloarchaea and their nanosized DPANN symbionts. Excellent collaboration with @deemteam.bsky.social, @anagtz.bsky.social and Michail Yakimov
Free access link: rdcu.be/eLtCH
🧵 by @yifanzhou.bsky.social 👇
October 17, 2025 at 10:28 AM
Reposted by Mart Krupovic
Now with the correct link:
We reviewed the "Molecular Mechanisms of Poxvirus Evolution"
We show that poxviruses have highly adaptable genomes, and are not sluggishly evolving, as often described.
journals.asm.org/doi/10.1128/...
October 16, 2025 at 7:20 PM
Reposted by Mart Krupovic
Very excited to share the latest work from our lab, which was published today in Nature!
nature.com/articles/s41...

PhD graduate and now post-doc Sofia Dahlman, along with co-senior author Sam Forster from The Hudson and other researchers from our lab and others.
Isolation, engineering and ecology of temperate phages from the human gut - Nature
Human host-associated cellular products may act as induction agents for bacteriophages.
nature.com
October 15, 2025 at 9:41 PM
Reposted by Mart Krupovic
Live cell imaging of Ca. Nha. antarcticus and Hrr. lacusprofundi using agarose pads. Protocol now live!
www.archaea.bio/protocols/li...
Live Cell Imaging of Ca. Nha. antarcticus and Hrr. lacusprofundi using agarose pads
This protocol is an adapted form of the protocol developed for imaging haloarchaea (Liao et al., 2021) and subsequently applied to co-cultures containing nanohaloarchaea and haloarchaea (Hamm et al., ...
www.archaea.bio
October 15, 2025 at 11:19 AM
Reposted by Mart Krupovic
The latest preprint from the lab is out!
Here we used structural clustering and immunoprecipitations to uncover some networks in our knowledge of giant virons proteomes. An interesting story by Hela Safi and @ambsch.bsky.social.
www.biorxiv.org/content/10.1...
Elucidating the interaction network of one of the largest icosahedral capsids in the virosphere
Giant viruses challenge traditional boundaries of virology with their large particle sizes, complex genomes, and unique replication strategies. Yet, despite its 750 nm diameter and incorporation of do...
www.biorxiv.org
October 15, 2025 at 5:19 AM
Reposted by Mart Krupovic
Our Pyrodictium cannulae paper is out in its final form! Cannulae assemble into calcium-stabilized tubular filaments stable at 100 °C—a unique archaeal cell–cell connection. Led brilliantly by @mikesleutel.bsky.social & @vinceconticello.bsky.social.

www.nature.com/articles/s41467-025-64120-8
October 14, 2025 at 2:25 PM
Reposted by Mart Krupovic
We are delighted to be at the FINstruct meeting today in Helsinki - dedicated to the memory of Dennis Bamford

instruct-eric.org/news/in-memo... @helsinki.fi @oulu.fi
October 14, 2025 at 6:39 AM
Reposted by Mart Krupovic
Our work on ribosome hibernation in archaea is out!
We identified Hib, a new hibernation factor broadly distributed across archaea.
Check out the preprint 👉 biorxiv.org/content/10.1101/2025.10.11.676729v1
(1/🧵)
October 13, 2025 at 4:26 PM
Reposted by Mart Krupovic
Phages evolve fast, or do they?
In oysters, some stay identical for years.
With >1,200 phages & 600 Vibrio genomes, we reveal long-term stability and new mobile elements.
Proud of this collaborative work across our teams (Roscoff-UdeM and @epcrocha.bsky.social www.biorxiv.org/cgi/content/...
Ecological constraints foster both extreme viral-host lineage stability and mobile element diversity in a marine community
Phages are typically viewed as very rapidly evolving biological entities. Little is known, however, about whether and how phages can establish long-term genetic stability. We addressed this eco-evolut...
www.biorxiv.org
October 12, 2025 at 9:16 PM
Reposted by Mart Krupovic
For those of you working on tailed #phages, this is the virus realm for you!
#Duplodnaviria #virus #taxonomy

The Profile in #JGenVirol provides the citable reference. More details in the chapter on the ICTV website: ictv.global/report/chapt...
October 10, 2025 at 8:07 AM
Reposted by Mart Krupovic
🚀 Released gbdraw v0.6.0!

- Added dinucleotide skew plotting to linear mode. Fixed the bug where only GC skew could be drawn.

- Added links to relevant sections of the documentation throughout the web app help menu.

Try out:👉 gbdraw.streamlit.app
October 9, 2025 at 2:01 PM