A sustained decrease in low-density lipoprotein cholesterol (LDL-C) is key to reducing the risk of atherosclerotic cardiovascular disease; however, achieving this goal can be hampered by low adherence...

Lactate elevations are a hallmark of human disease, but the mechanisms regulating lactate levels remain poorly understood. Here, Lee et al. elucidate the mechanisms of lactate homeostasis. These invol...

Petrovic et al. demonstrate that ergothioneine acts as an alternative substrate for cystathionine gamma-lyase, an H2S-producing enzyme, leading to an increase of protein persulfidation. The persulfida...

A nanoflow multiomic workflow reveals how lysosomal dysfunction disrupts autophagy and mitochondrial homeostasis in LSD mutants.

Top-down mass spectrometry (MS) enables comprehensive characterization of modified proteins and nucleic acids and, when native electrospray ionization (ESI) is used, binding site mapping of their complexes with native or therapeutic ligands. However, the high complexity of top-down MS spectra poses a serious challenge to both manual and automated data interpretation, even when the protein, RNA, or DNA sequence and the type of modification or the ligand are known. Here, we introduce FAST MS, a user-friendly software that identifies, assigns and relatively quantifies signals of molecular and fragment ions in MS and MS/MS spectra of biopolymers with known sequence and provides a toolbox for statistical analysis. FAST MS searches mass spectra for ion signals by comparing all signals in the spectrum with isotopic profiles calculated from known sequences, resulting in superior sensitivity and an increased number of assigned fragment ions compared to algorithms that rely on artificial monomer units while maintaining the false positive rate on a moderate level (<5%). FAST MS is an open-source, cross-platform software for the accurate identification, localization and relative quantification of modifications, even in complex mixtures of positional isomers of proteins, oligonucleotides, or any other user-defined linear polymer.

Background The Intensive Diet and Exercise for Arthritis (IDEA) trial was a randomized trial conducted to evaluate the effects of diet and exercise on osteoarthritis (OA), the most prevalent form of a...

ACLY-deficient CAR T cells targeting the tumor-associated antigen disialoganglioside GD2 (GD2 CAR T cells) also resisted acquiring TEX-like properties, exhibiting reduced CD39 expression when co-cultu...

We present phi-4, a 14-billion parameter language model developed with a training recipe that is centrally focused on data quality. Unlike most language models, where pre-training is based primarily o...

Understanding and targeting these Vascular Adhesion Molecules are hoped to substantially contribute to improving the prevention and biomarker-guided treatment of Cardiovascular Diseases.