SPACEtomo v1.2 is online! github.com/eisfabian/SP...

- Improved GUIs for lamella detection, target selection and CLEM registration make it the best target selection workflow for PACEtomo on any kind of lamella sample.
- Easier installation and configuration

Any feedback is much appreciated! :)

Ever wondered how transcription choreographs histone modifications? Our work reveals the basis of co-transcriptional H3K36me3 by SETD2. We visualize how a histone writer coordinates with the transcription machinery! This is the magnus opus of @jonmarkert.bsky.social!
tinyurl.com/setd2

Just for fun, reprocessed a subset (1200 mics) of EMPIAR-11918 (beautiful 40kDa RNA-protein complex) with cryoSPARC to compare with BLUSH/RELION workflow - 3Å NU-refine from 60k blob-picked particles after quick classification without alignments

Oh my. This is just gorgeous from Misha Kudryashev's lab (I don't think he's here yet?). Love to see it. V ATPase resolved directly in synaptic vesicles 🤤 Lots of other neat observations in there also. Congrats to all involved! www.pnas.org/doi/10.1073/...

Do not underestimate the importance of small proteins.
www.science.org/content/arti...

Thanks to all our amazing collaborators in Japan🇯🇵 (Ginga, Yusuke, Aki, Genji), UK🇬🇧 (Onyou, Sabina, @jnb-lab.bsky.social, Luke) & France🇫🇷 (@serenaflori.bsky.social, Giovanni). And huge congrats to Manon Demulder!👩‍🔬💪

Here's a beautiful #NotTheCover of diatoms on a grid by Martin Oeggerli #SciArt🧪🎨 7/🧵

A fun journey of full-length EGFR with Dan Leahy and
@edwardmarcotte.bsky.social at UT Austin @utaustin.bsky.social. See below:
Structure and organization of full-length Epidermal Growth Factor Receptor in extracellular vesicles by cryo-electron tomography www.biorxiv.org/content/10.1...

Very excited to share the first story from my postdoc where we uncover the structural basis for slow Cas9 turnover!

We use kinetics-guided cryo-EM to capture 25 conformational states of SpCas9 from a single dataset - including the first look at Cas9 releasing product.

biorxiv.org/content/10.1...

This one was delayed for a while… apologies to the musicians. Collab with Matt, Will and Lorna (over on Twitter). And a great dev effort from Lorenz @lorenzlamm.bsky.social. Ofc @cellarchlab.com team. Comment if you want to help. #teamthylakoid

www.biorxiv.org/content/10.1...

Faster and more accurate protein modeling with CryFold. By focusing on nearby points (Local Attention) and employing map masking, CryFold excels in generating models from challenging maps regions. It enables a more cost-efficient model building. 🧵
💾 github.com/SBQ-1999/Cry...

Thrilled to announce Boltz-1, the first open-source and commercially available model to achieve AlphaFold3-level accuracy on biomolecular structure prediction! An exciting collaboration with Jeremy, Saro, and an amazing team at MIT and Genesis Therapeutics. A thread!

Our new preprint from Briggs lab is out! We solved the in-virus #cryoEM structure of the HIV matrix protein by SPA. The structure led us to a discovery resolving a long mystery in HIV biology – a peptide SP2 triggers the maturation of the HIV matrix domain. A 🧵 1/x
Paper: tinyurl.com/c7szhtfa

Meet the PyShell!🥧🐚

Diatom pyrenoids fix up to 20% of the Earth's #CO2🌏. We discovered that their Rubisco is encased in a tight protein lattice that is essential for efficient carbon fixation and cell growth🌱

Super excited to share our new preprint📜: www.biorxiv.org/content/10.1...

🌾🧪 🧶🧬 ❄️🔬