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wojwie.bsky.social
woj_wie
@wojwie.bsky.social
790 followers 56 following 5 posts
Photosynthesis and cryoEM at Biozentrum, Basel.
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Reposted by woj_wie
profmattjohnson.bsky.social
Our new preprint 🚨🌱☀️on flourescent biosensing of ATP levels in chloroplasts in vivo shows disequilibrium between proton motive force and ATP levels. We show PGR5 dependent cyclic electron transfer does not augment ATP/NADPH ratios @gustaf23.bsky.social 🙌

www.biorxiv.org/cgi/content/...
November 12, 2025 at 3:42 PM
wojwie.bsky.social
It’s strange. I know it’s just an option and not the first AI-assist. but imo the main positive sounds like endorsing scientist to trust opinions of a company(?)-run blackbox. A posterior rather than during design. And it’s coming from the place that to many was the first step to improve publishing…
November 7, 2025 at 9:08 PM
Reposted by woj_wie
manondemulder.bsky.social
We’re back with the next DinoSphere Online Seminar!

Join us on Nov 4th, we're hosting:

Edmée Royen (ULiège) - Symbiodinium

Nicolas dos Santos Pacheco (Cambridge) - Perkinsids

📅 Tue, Nov 4 - 4 PM CET/3 PM GMT

💻 Zoom link: sites.google.com/view/dinosphere


Please spread the word!
#protistonsky
October 28, 2025 at 3:29 PM
Reposted by woj_wie
mpibp.bsky.social
Have you registered to the next EMBO Practical Course on In-situ structural biology by Cryo-FIB and Cryo-ET? 🧊 Join us in Frankfurt for hands-on training on cryo-techniques and networking with experts!

🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...
📍 Frankfurt | 📆 April 12–20, 2026
Orange and green poster of the In-situ structural biology by Cryo-FIB and Cryo-ET in Frankfurt am Main, with EMBO logo. The poster promotes childcare grants, travel grants and that this is a sustainable event.
October 23, 2025 at 9:10 AM
Reposted by woj_wie
labvanni.bsky.social
Thanks to @snsf.ch, we are hiring two PhD students to investigate frontier topics in lipid biology using a combination of MD simulations and experiments. Preferred starting date: ASAP. Fribourg - and Switzerland - are quite nice, for both work and private life! Please spread the word!
October 13, 2025 at 2:17 PM
Reposted by woj_wie
biozentrum.unibas.ch
Assistant/Associate Professor in Structural Biology, Biophysics or Biological Imaging

www.biozentrum.unibas.ch/open-positio...

#biozentrum #University #Basel #Professor #Structural #Biology #Biophysics #Biological #Imaging
September 29, 2025 at 2:58 PM
Reposted by woj_wie
cellarchlab.com
Time for a thread!🧵 How different is the molecular organization of thylakoids in “higher” plants🌱? To find out, we teamed up with @profmattjohnson.bsky.social to dive into spinach chloroplasts with #CryoET ❄️🔬. Curious? ..Read on!

#TeamTomo #PlantScience 🧪 🧶🧬 🌾
elifesciences.org/articles/105...
1/🧵
September 25, 2025 at 6:00 PM
Reposted by woj_wie
svenklumpe.bsky.social
Extremely excited for the second version of this workshop at the MPI of Biophysics l, Frankfurt, that will happen 12-20. April 2026. Absolutely amazing lineup of speakers, I am very much looking forward to learning from everyone! Apply here: meetings.embo.org/event/26-in-...
August 15, 2025 at 4:09 PM
Reposted by woj_wie
biozentrum.unibas.ch
Apply now for the prestigious and independent #BiozentrumPhDFellowships. Great science. Unique rotation-based selection of research group and other incentives. The summer call is open until October 12, 2025. bit.ly/4caiqqX @biozentrum.unibas.ch @unibas.ch #fellowship #PhD#Switzerland
Biozentrum PhD Fellowships
Share your passion for life sciences. If you are talented and highly motivated, want to broaden your horizons and are interested in a wide range of research topics, apply for one of the sought after B...
bit.ly
August 4, 2025 at 11:40 AM
Reposted by woj_wie
manondemulder.bsky.social
Our first DinoSphere seminar is this Tuesday, 1 July at 9 AM CEST! A good way to start the day if you're in Europe ☕

We will hear from Yong Heng Phua (OIST) and Karel Mocaer (Heidelberg)
🔗 Zoom link on our website: tinyurl.com/4duvwtvp
DinoSphere - Online talks
Video credits: Liz Cooney
tinyurl.com
June 30, 2025 at 1:07 PM
Reposted by woj_wie
florianwollweber.bsky.social
After 5 amazing years @pilhoferlab.bsky.social I'm starting my lab @embl.org Grenoble this November embl.org/wollweber
We'll image Asgard archaea and many other strange microbes ( #archaeasky, #protistsonsky..) to understand eukaryogenesis
First job ad: #teamtomo scientist!🔬❄️ tinyurl.com/2rdu2ze6
June 27, 2025 at 10:58 AM
Reposted by woj_wie
manondemulder.bsky.social
We’re kicking off the DinoSphere Online Seminar Series! Join us for our first session with Karel Mockaer (Heidelberg) & Yong Heng Phua (OIST)

📅 1 July 9AM CEST
🔗 tinyurl.com/4mjaverj

Spread the word!
@protistwtmostest.bsky.social @ehehenberger.bsky.social @chandnibhickta.bsky.social&Norico Yamada
June 4, 2025 at 4:34 AM
Reposted by woj_wie
profmattjohnson.bsky.social
Pleased to share my very general review of Photosynthesis now published in Nature Reviews Mol Cell Biol
🌞🌱
rdcu.be/em1lz Hopefully a useful primer for Postgrad and undergrad students
Structure, regulation and assembly of the photosynthetic electron transport chain
Nature Reviews Molecular Cell Biology - The electron transfer chain in chloroplast thylakoid membranes uses solar energy to produce NADPH and ATP, which power carbon fixation into biomass. This...
rdcu.be
May 21, 2025 at 5:40 PM
Reposted by woj_wie
georgealfredcurrie.bsky.social
Does publishing serve science or is science serving publishing?

Damian Pattinson and I (@elife.bsky.social) argue scientific publishing has evolved into a system that, rather than facilitate scholarly communication, distorts and dictates it.

onlinelibrary.wiley.com/doi/10.1002/...

#OpenScience
Screenshot of article summary of: Toward Science-Led Publishing by Damian Pattinson, George Currie published as an opinion piece, in Learned Publishing Summary The current dynamic of scholarly publishing prioritises the wants of the publishing industry over the needs of the research community. This article explores this theme through the lens of ‘publisher-led science’ as a description of our current status quo, and through ‘science-led publishing’ as an improved future state. We argue that financial motivations central to most publishing distort how research is presented, how it is assessed and even what research is undertaken, leading to a system that hinders, rather than facilitates, scientific progress. We propose three elements of a science-led publishing approach that would accelerate research communication, incentivise collaboration between authors, editors and reviewers, and create a more transparent and equitable research landscape. We believe that research funding and research assessment are two of the primary levers for wider change in research and research culture and consider the future purpose of scholarly publishing in a world where these proposals have been widely adopted.
May 19, 2025 at 2:52 PM
Reposted by woj_wie
phaips.vd.st
Hey #TeamTomo,
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!

tomoguide.github.io

Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Welcome to TomoGuide
A step-by-step Cryo-ET guide
tomoguide.github.io
May 6, 2025 at 4:27 PM
Reposted by woj_wie
florentwaltz.bsky.social
You want to start tomography? Solve structures inside cells? Reach Nyquist 😳 ? @phaips.vd.st and I have a website for you! tomoguide.github.io
You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software!
Hope it will be useful !
3.8 angstrom resolution ribosome from 33 tomograms The TomoGuide website
May 6, 2025 at 4:42 PM
Reposted by woj_wie
lorenzlamm.bsky.social
🦠🧠 MemBrain update! 🧠🦠
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo
April 25, 2025 at 7:28 AM
Reposted by woj_wie
3p1l.bsky.social
Hey Everyone!❄️🔬 We have two fully funded PhD positions @humantechnopole.bsky.social this year!
Help us understand better how one of the main stress regulators - the nucleolus - integrates synaptic inputs!🧠Find all the calls - also from my amazing colleagues - here: www.semm.it/education/ho...
April 22, 2025 at 12:44 PM
Reposted by woj_wie
florianwollweber.bsky.social
Asgard archaea are full of surprises, this time we found (tiny) microtubules (snapshots of microtubule evolution?!) - Check out our new paper on the cytoskeleton of Lokiarchaeum ossiferum: www.cell.com/cell/fulltex...
pilhoferlab.bsky.social Pilhofer Lab 🦠❄️🔬 @pilhoferlab.bsky.social · Mar 21
Asgard archaea have actin - but what about microtubules? Where do they come from? 🧐 Our new paper www.cell.com/cell/fulltex... by @xujwet.bsky.social & @florianwollweber.bsky.social, in collaboration with the Schleper & Wieczorek labs, describes tiny Asgard microtubules! #TeamTomo #ArchaeaSky 1/6
Credit: Margot Riggi (@margotriggi.bsky.social), Max-Planck Institute of Biochemistry (@mpibiochem.bsky.social)
March 21, 2025 at 4:15 PM
Reposted by woj_wie
florentwaltz.bsky.social
The final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in @science.org 🎉
You can find the full story here www.science.org/doi/10.1126/...
@verenaresch.bsky.social did a wonderful job on the cover and animation, she really brought it to life 👩‍🎨
science.org Science Magazine @science.org · Mar 20
In a new Science study, cryo–electron tomography captures the in-cell architecture of the mitochondrial respiratory chain, illuminating how the coordinated action of molecular machines drives life’s fundamental energy conversion.

Learn more in this week's issue: scim.ag/3FA3Ygq
March 20, 2025 at 6:20 PM
Reposted by woj_wie
kgeissler.bsky.social
Check out @deglushk.bsky.social 's method to measure membrane thickness in cryo electron tomograms!
biorxivpreprint.bsky.social bioRxivpreprint @biorxivpreprint.bsky.social · Mar 14
In situ evidence for systematic membrane thickness variation across cellular organelles https://www.biorxiv.org/content/10.1101/2025.03.13.642912v1
March 15, 2025 at 2:25 PM
Reposted by woj_wie
richardsever.bsky.social
Big news: we are setting up a new non-profit organization to run bioRxiv and medRxiv. It's called openRxiv [no it's not a new preprint server; it's dedicated organization to oversee the servers] openrxiv.org 1/n
Homepage - openRxiv
openRxiv is an independent non-profit, the new organizational home for bioRxiv and medRxiv, enabling researchers to instantly share groundbreaking findings with the global scientific community.
openrxiv.org
March 11, 2025 at 1:20 PM
Reposted by woj_wie
carlbergstrom.com
Let's talk about this Nature piece in more detail.

I've rarely read something so anti-scientific anywhere short of the National Review.

www.nature.com/articles/d41...
Three AI-powered steps to faster, smarter peer review
Tired of spending countless hours on peer reviews? An AI-assisted workflow could help.
www.nature.com
March 6, 2025 at 5:34 AM
Reposted by woj_wie
svenklumpe.bsky.social
Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?
March 5, 2025 at 4:02 PM
Reposted by woj_wie
lifeonthewedge.bsky.social
To all #TeamTomo #CryoET and #Chlamydomonas aficionados: we have updated EMPIAR-11830 with a bug fix concerning some cryo-CARE denoised tomos as well as additional files and metadata! 🎉👩🏽‍💻

www.ebi.ac.uk/empiar/EMPIA...

A little thread about what's new... 🧵 1/n
Rare cellular events observed in the cryo-ET dataset. Slices through tomograms (left) with corresponding 3D segmentation (middle and right). Right panels show enlarged views of the boxed regions in the middle panels. A) Mitochondrion at the plasma membrane, tethered to microtubules by thin filaments of unknown identity. Segmented classes: nuclear envelope (light blue), nuclear pore complex (dark blue), 80S ribosomes (orange), mitochondrial membranes (dark red), ATP synthase (royal blue), plasma membranes (grey), microtubules (pink), filaments (yellow), endoplasmic reticulum (purple). Zoomed-in panel shows thin filaments running between microtubules and the mitochondrion at the plasma membrane. Note that this tomogram contains two closely appressed cells, and thus, two plasma membranes with cell walls between. B) Mitochondrial fission event with ER membrane interactions. Segmented classes: mitochondrial membranes (dark red), ATP synthase (royal blue), 80S ribosomes (orange), thylakoid membranes (green), PSII (bright yellow), cell wall (grey), ER (purple), fission site containing filamentous structures perpendicular to the mitochondrial long axis (pale yellow). C) Ciliary transition zone between basal body and axoneme, including assembling IFT train and stellate structure77. Segmented classes: microtubule doublets (light blue), central microtubule pair (light green), stellate structure (pale red), IFT train (yellow). D) Pyrenoid tubule extending from the thylakoids into the phase-separated Rubisco matrix of the pyrenoid. Minitubules originate from thylakoid membranes82. Segmented classes: thylakoid membranes (dark green), PSII (yellow), pyrenoid tubule (lime green), Rubisco (lavender blue), minitubules (orange). Scale bars in A-D: 100 nm. Related to Fig. 2C-H.
February 28, 2025 at 4:35 PM