Florian Wollweber
@florianwollweber.bsky.social
Imaging Asgard archaea and other strange organisms @embl.org Grenoble | 🦠❄️🔬#teamtomo
embl.org/wollweber
embl.org/wollweber
Pinned
Asgard archaea have actin - but what about microtubules? Where do they come from? 🧐 Our new paper www.cell.com/cell/fulltex... by @xujwet.bsky.social & @florianwollweber.bsky.social, in collaboration with the Schleper & Wieczorek labs, describes tiny Asgard microtubules! #TeamTomo #ArchaeaSky 1/6
Asgard archaea are full of surprises, this time we found (tiny) microtubules (snapshots of microtubule evolution?!) - Check out our new paper on the cytoskeleton of Lokiarchaeum ossiferum: www.cell.com/cell/fulltex...
Reposted by Florian Wollweber
The abstract submission deadline for our upcoming 'Imaging Cell Dynamics' meeting is this Friday. We still have a few places remaining so apply now to secure your spot!
Find out more at:
www.biologists.com/meetings/jcs...
#imaging #microscopy #cellbiology
Find out more at:
www.biologists.com/meetings/jcs...
#imaging #microscopy #cellbiology
February 11, 2026 at 4:19 PM
The abstract submission deadline for our upcoming 'Imaging Cell Dynamics' meeting is this Friday. We still have a few places remaining so apply now to secure your spot!
Find out more at:
www.biologists.com/meetings/jcs...
#imaging #microscopy #cellbiology
Find out more at:
www.biologists.com/meetings/jcs...
#imaging #microscopy #cellbiology
Reposted by Florian Wollweber
Only a week left to apply for the 'Structural characterisation of macromolecular complexes' course at EMBL Grenoble! Learn about the strategic use of cutting-edge protein structure prediction methods and recent advances in in situ structural biology. 🧬 #EMBOMacromolecular
s.embl.org/mmo26-01-bl
s.embl.org/mmo26-01-bl
February 11, 2026 at 9:20 AM
Only a week left to apply for the 'Structural characterisation of macromolecular complexes' course at EMBL Grenoble! Learn about the strategic use of cutting-edge protein structure prediction methods and recent advances in in situ structural biology. 🧬 #EMBOMacromolecular
s.embl.org/mmo26-01-bl
s.embl.org/mmo26-01-bl
Reposted by Florian Wollweber
Contractile Injection Systems - Hot Springs - cryoET: Read the final version of our paper here:
academic.oup.com/ismej/advanc...
#microsky #teamtomo
academic.oup.com/ismej/advanc...
#microsky #teamtomo
Thermophilic bacteria employ a contractile injection system in hot spring microbial mats
Abstract. Bacterial contractile injection systems (CISs) are multiprotein complexes that facilitate the bacterial response to environmental factors or inte
academic.oup.com
February 11, 2026 at 2:43 PM
Contractile Injection Systems - Hot Springs - cryoET: Read the final version of our paper here:
academic.oup.com/ismej/advanc...
#microsky #teamtomo
academic.oup.com/ismej/advanc...
#microsky #teamtomo
Reposted by Florian Wollweber
Long in the making, but happy to present the Chlamydomonas chlororibosome!
Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins.
bioRxiv 📖: shorturl.at/q44tG
This suggests greater chlororibosome diversity than expected!
1/n 🧵
Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins.
bioRxiv 📖: shorturl.at/q44tG
This suggests greater chlororibosome diversity than expected!
1/n 🧵
February 10, 2026 at 8:35 AM
Long in the making, but happy to present the Chlamydomonas chlororibosome!
Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins.
bioRxiv 📖: shorturl.at/q44tG
This suggests greater chlororibosome diversity than expected!
1/n 🧵
Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins.
bioRxiv 📖: shorturl.at/q44tG
This suggests greater chlororibosome diversity than expected!
1/n 🧵
Reposted by Florian Wollweber
Proud and excited to share our work with the community! Helen @helenfoster.bsky.social Foster et al. study how #cilia axonemes are built. 👀 Don't miss the gorgeous and insightful movies by @margotriggi.bsky.social, they really show our discoveries in full action!
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
February 9, 2026 at 8:03 PM
Proud and excited to share our work with the community! Helen @helenfoster.bsky.social Foster et al. study how #cilia axonemes are built. 👀 Don't miss the gorgeous and insightful movies by @margotriggi.bsky.social, they really show our discoveries in full action!
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Reposted by Florian Wollweber
There was so much to unpack with this data, we teamed up with the marvellous @margotriggi.bsky.social to help explain what we think is going on. (Please also check out our model of central apparatus assembly here: www.biorxiv.org/content/10.6...)
February 6, 2026 at 11:20 AM
There was so much to unpack with this data, we teamed up with the marvellous @margotriggi.bsky.social to help explain what we think is going on. (Please also check out our model of central apparatus assembly here: www.biorxiv.org/content/10.6...)
Reposted by Florian Wollweber
Very happy to say that our work on how axonemes are generated is now available on bioRxiv: www.biorxiv.org/content/10.6... - if you’ve ever wondered how the incredibly beautiful, complex structures within motile #cilia are constructed then this is for you!
www.biorxiv.org
February 6, 2026 at 11:20 AM
Very happy to say that our work on how axonemes are generated is now available on bioRxiv: www.biorxiv.org/content/10.6... - if you’ve ever wondered how the incredibly beautiful, complex structures within motile #cilia are constructed then this is for you!
Reposted by Florian Wollweber
🌀 How do spirochete bacteria swim through thick fluids like champions?
We solved T. denticola flagella structure - asymmetric proteins expand one side, compress the other for perfect corkscrew motion!
@debnathghosal.bsky.social
🔗 doi.org/10.64898/202...
#StructuralBiology #CryoEM #Microbiology
We solved T. denticola flagella structure - asymmetric proteins expand one side, compress the other for perfect corkscrew motion!
@debnathghosal.bsky.social
🔗 doi.org/10.64898/202...
#StructuralBiology #CryoEM #Microbiology
February 5, 2026 at 6:37 AM
🌀 How do spirochete bacteria swim through thick fluids like champions?
We solved T. denticola flagella structure - asymmetric proteins expand one side, compress the other for perfect corkscrew motion!
@debnathghosal.bsky.social
🔗 doi.org/10.64898/202...
#StructuralBiology #CryoEM #Microbiology
We solved T. denticola flagella structure - asymmetric proteins expand one side, compress the other for perfect corkscrew motion!
@debnathghosal.bsky.social
🔗 doi.org/10.64898/202...
#StructuralBiology #CryoEM #Microbiology
Reposted by Florian Wollweber
📣 New paper alert! This is a story of molecular tricks that let spirochetes (spiral-shaped bacteria) drill through tissue. www.biorxiv.org/content/10.6...
February 5, 2026 at 2:19 PM
📣 New paper alert! This is a story of molecular tricks that let spirochetes (spiral-shaped bacteria) drill through tissue. www.biorxiv.org/content/10.6...
Reposted by Florian Wollweber
I've been working on Surface Morphometrics for a new version (coming soon), and made some significant (AI-assisted) performance improvements, especially in pycurv (20-50x!). If you've had technical issues or just felt like it was too slow, give it another try! #teamtomo github.com/GrotjahnLab/...
GitHub - GrotjahnLab/surface_morphometrics: Morphometrics for Membrane Surfaces Segmented from Cryo-ET or other volumetric imaging.
Morphometrics for Membrane Surfaces Segmented from Cryo-ET or other volumetric imaging. - GrotjahnLab/surface_morphometrics
github.com
February 4, 2026 at 12:32 AM
I've been working on Surface Morphometrics for a new version (coming soon), and made some significant (AI-assisted) performance improvements, especially in pycurv (20-50x!). If you've had technical issues or just felt like it was too slow, give it another try! #teamtomo github.com/GrotjahnLab/...
Reposted by Florian Wollweber
Extremophile alert!
Registration is OPEN for the 2026 EMBO Archaeal workshop in Cambridge.
Spaces are limited, so register soon with an abstract if you’d like to give a talk.
If you can’t come in person, we are welcoming virtual attendees. So join us online where all talks will be screened!
Registration is OPEN for the 2026 EMBO Archaeal workshop in Cambridge.
Spaces are limited, so register soon with an abstract if you’d like to give a talk.
If you can’t come in person, we are welcoming virtual attendees. So join us online where all talks will be screened!
Sign up now for the EMBO Workshop "#MolecularBiology of #Archaea: Life Through the Prism of Archaea" in Cambridge, UK, 6–10 July 2026.
Deadline: 15 April
https://meetings.embo.org/event/26-archaea
#EMBOArchaeaBiology #EMBOevents #CellBiology #StructuralBiology #ecology #evolution #biophysics 🧪
Deadline: 15 April
https://meetings.embo.org/event/26-archaea
#EMBOArchaeaBiology #EMBOevents #CellBiology #StructuralBiology #ecology #evolution #biophysics 🧪
February 5, 2026 at 3:58 PM
Extremophile alert!
Registration is OPEN for the 2026 EMBO Archaeal workshop in Cambridge.
Spaces are limited, so register soon with an abstract if you’d like to give a talk.
If you can’t come in person, we are welcoming virtual attendees. So join us online where all talks will be screened!
Registration is OPEN for the 2026 EMBO Archaeal workshop in Cambridge.
Spaces are limited, so register soon with an abstract if you’d like to give a talk.
If you can’t come in person, we are welcoming virtual attendees. So join us online where all talks will be screened!
Reposted by Florian Wollweber
Thx for @mollyherring.bsky.social for featuring our recent work with @centriolelab.bsky.social and our quest with @gautamdey.bsky.social & the support of the @moorefound.bsky.social to look for the beauty in every #eukaryote out there using #Expansion #Microscopy #UExM
Even with the largest zoom on a classic compound optical system, scientists struggle to make sense of finer details. Microbiologists are turning to an unexpected source to clear things up: a moisture-absorbing material found in diapers. www.quantamagazine.org/expansion-mi...
Expansion Microscopy Has Transformed How We See the Cellular World | Quanta Magazine
How physically magnifying objects using a key ingredient in diapers has opened an unprecedented view of the microbial world.
www.quantamagazine.org
February 4, 2026 at 7:30 PM
Thx for @mollyherring.bsky.social for featuring our recent work with @centriolelab.bsky.social and our quest with @gautamdey.bsky.social & the support of the @moorefound.bsky.social to look for the beauty in every #eukaryote out there using #Expansion #Microscopy #UExM
Reposted by Florian Wollweber
We are hiring for group leaders again — EBI is a great place to start your research group!
embl.wd103.myworkdayjobs.com/EMBL/job/Hin...
embl.wd103.myworkdayjobs.com/EMBL/job/Hin...
Research Group Leader
Do you want to lead groundbreaking research in computational biology? Join us at EMBL-EBI! EMBL's European Bioinformatics Institute (EMBL-EBI) is seeking talented and highly-motivated scientists to jo...
embl.wd103.myworkdayjobs.com
January 30, 2026 at 9:02 AM
We are hiring for group leaders again — EBI is a great place to start your research group!
embl.wd103.myworkdayjobs.com/EMBL/job/Hin...
embl.wd103.myworkdayjobs.com/EMBL/job/Hin...
Reposted by Florian Wollweber
Excited to share this detailed biophysical study of #PhaseSeparation in the Pyrenoid, an algal microcompartment that fixes a third of the Earth's #CO2 🌏. We combine methods across scales, in vitro 🧪 and in vivo 🦠
Thanks for the very fun collaboration! 🤗
#PlantScience 🌾 #TeamTomo 🔬 #LivingPhysics 🧬🧶
Thanks for the very fun collaboration! 🤗
#PlantScience 🌾 #TeamTomo 🔬 #LivingPhysics 🧬🧶
How does molecular valency shape condensate assembly and function? We used the CO2-fixing organelle in algae—the pyrenoid—to find out… 🧵
Preprint here!:
www.biorxiv.org/content/10.6...
@cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social
#Rubisco #PhaseSeparation #Condensates #Pyrenoid
Preprint here!:
www.biorxiv.org/content/10.6...
@cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social
#Rubisco #PhaseSeparation #Condensates #Pyrenoid
January 30, 2026 at 3:50 PM
Excited to share this detailed biophysical study of #PhaseSeparation in the Pyrenoid, an algal microcompartment that fixes a third of the Earth's #CO2 🌏. We combine methods across scales, in vitro 🧪 and in vivo 🦠
Thanks for the very fun collaboration! 🤗
#PlantScience 🌾 #TeamTomo 🔬 #LivingPhysics 🧬🧶
Thanks for the very fun collaboration! 🤗
#PlantScience 🌾 #TeamTomo 🔬 #LivingPhysics 🧬🧶
Reposted by Florian Wollweber
FoldMason is out now in @science.org. It generates accurate multiple structure alignments for thousands of protein structures in seconds. Great work by Cameron L. M. Gilchrist and @milot.bsky.social.
📄 www.science.org/doi/10.1126/...
🌐 search.foldseek.com/foldmason
💾 github.com/steineggerla...
📄 www.science.org/doi/10.1126/...
🌐 search.foldseek.com/foldmason
💾 github.com/steineggerla...
Multiple protein structure alignment at scale with FoldMason
Protein structure is conserved beyond sequence, making multiple structural alignment (MSTA) essential for analyzing distantly related proteins. Computational prediction methods have vastly extended ou...
www.science.org
January 30, 2026 at 6:11 AM
FoldMason is out now in @science.org. It generates accurate multiple structure alignments for thousands of protein structures in seconds. Great work by Cameron L. M. Gilchrist and @milot.bsky.social.
📄 www.science.org/doi/10.1126/...
🌐 search.foldseek.com/foldmason
💾 github.com/steineggerla...
📄 www.science.org/doi/10.1126/...
🌐 search.foldseek.com/foldmason
💾 github.com/steineggerla...
Reposted by Florian Wollweber
Love seeing the archaeal work at EMBL highlighted! 🥰 Feels great to be part of a growing community pushing this fascinating biology forward! #teamArchaea #EMBL 🧫🤓🧪🌏
Archaea may not be well known, nor well studied, but these microorganisms can live in the most extreme environments, but also right on our skin. They’re everywhere.
EMBL researchers are now exploring their unique ecosystem adaptability and link to evolution.
www.embl.org/news/science...
EMBL researchers are now exploring their unique ecosystem adaptability and link to evolution.
www.embl.org/news/science...
January 29, 2026 at 9:43 AM
Love seeing the archaeal work at EMBL highlighted! 🥰 Feels great to be part of a growing community pushing this fascinating biology forward! #teamArchaea #EMBL 🧫🤓🧪🌏
Reposted by Florian Wollweber
When you forget to turn on high-res interpolation in 3dmod
January 29, 2026 at 12:35 PM
When you forget to turn on high-res interpolation in 3dmod
Reposted by Florian Wollweber
Archaea may not be well known, nor well studied, but these microorganisms can live in the most extreme environments, but also right on our skin. They’re everywhere.
EMBL researchers are now exploring their unique ecosystem adaptability and link to evolution.
www.embl.org/news/science...
EMBL researchers are now exploring their unique ecosystem adaptability and link to evolution.
www.embl.org/news/science...
January 29, 2026 at 9:36 AM
Archaea may not be well known, nor well studied, but these microorganisms can live in the most extreme environments, but also right on our skin. They’re everywhere.
EMBL researchers are now exploring their unique ecosystem adaptability and link to evolution.
www.embl.org/news/science...
EMBL researchers are now exploring their unique ecosystem adaptability and link to evolution.
www.embl.org/news/science...
Reposted by Florian Wollweber
R u an undergraduate and wanna work in my lab - or another lab at MSU - in the summer? Apply to our NSF-funded Research Experiences for Undergraduates program 'Microbiology of Low Oxygen Ecosystems'! Expenses paid, competitive application, email me if you have questions. montana.edu/mbi/reu/
REU Program - Department of Microbiology & Cell Biology | Montana State University
www.montana.edu
January 28, 2026 at 8:29 PM
R u an undergraduate and wanna work in my lab - or another lab at MSU - in the summer? Apply to our NSF-funded Research Experiences for Undergraduates program 'Microbiology of Low Oxygen Ecosystems'! Expenses paid, competitive application, email me if you have questions. montana.edu/mbi/reu/
Reposted by Florian Wollweber
Looking forward to continuing to working with Florian on visualizing our Asgard archaeon 'Skadiarchaeum cthulhuensis'!
January 28, 2026 at 8:33 PM
Looking forward to continuing to working with Florian on visualizing our Asgard archaeon 'Skadiarchaeum cthulhuensis'!
Reposted by Florian Wollweber
The origin of eukaryotic cells continues to be one of the greatest mysteries in biology.
EMBL Grenoble is excited to welcome Florian Wollweber, who will use cutting-edge imaging techniques to study Asgard archaea — microbes closely related to our earliest ancestors. 🦠🔬
www.embl.org/news/people-...
EMBL Grenoble is excited to welcome Florian Wollweber, who will use cutting-edge imaging techniques to study Asgard archaea — microbes closely related to our earliest ancestors. 🦠🔬
www.embl.org/news/people-...
January 28, 2026 at 10:56 AM
The origin of eukaryotic cells continues to be one of the greatest mysteries in biology.
EMBL Grenoble is excited to welcome Florian Wollweber, who will use cutting-edge imaging techniques to study Asgard archaea — microbes closely related to our earliest ancestors. 🦠🔬
www.embl.org/news/people-...
EMBL Grenoble is excited to welcome Florian Wollweber, who will use cutting-edge imaging techniques to study Asgard archaea — microbes closely related to our earliest ancestors. 🦠🔬
www.embl.org/news/people-...
Reposted by Florian Wollweber
New on the CryoET Data Portal: ~27,000 tomograms of affinity-captured lysosomes from HEK293T cells across 4 physiological states. Includes raw data, AreTomo3 reconstructions & Membrain-Seg annotations. Openly available for reuse!
cryoetdataportal.czscience.com/depositions/...
cryoetdataportal.czscience.com/depositions/...
January 27, 2026 at 7:59 PM
New on the CryoET Data Portal: ~27,000 tomograms of affinity-captured lysosomes from HEK293T cells across 4 physiological states. Includes raw data, AreTomo3 reconstructions & Membrain-Seg annotations. Openly available for reuse!
cryoetdataportal.czscience.com/depositions/...
cryoetdataportal.czscience.com/depositions/...
Reposted by Florian Wollweber
SAVE THE DATE!
The next instalment of the long-running Bacterial and Archaeal Cell Division Conference will take place at www.marriott.com/en-us/hotels..., Italy from the 20th to 23rd of April 2027.
Organisers: me, @s-lab.bsky.social, @awehenkel.bsky.social.
More info soon.
#MicroSky
The next instalment of the long-running Bacterial and Archaeal Cell Division Conference will take place at www.marriott.com/en-us/hotels..., Italy from the 20th to 23rd of April 2027.
Organisers: me, @s-lab.bsky.social, @awehenkel.bsky.social.
More info soon.
#MicroSky
www.marriott.com
January 26, 2026 at 1:14 PM
SAVE THE DATE!
The next instalment of the long-running Bacterial and Archaeal Cell Division Conference will take place at www.marriott.com/en-us/hotels..., Italy from the 20th to 23rd of April 2027.
Organisers: me, @s-lab.bsky.social, @awehenkel.bsky.social.
More info soon.
#MicroSky
The next instalment of the long-running Bacterial and Archaeal Cell Division Conference will take place at www.marriott.com/en-us/hotels..., Italy from the 20th to 23rd of April 2027.
Organisers: me, @s-lab.bsky.social, @awehenkel.bsky.social.
More info soon.
#MicroSky
Reposted by Florian Wollweber
A reminder to sign up for the 2026 EMBO archaeal meeting im Cambridge: meetings.embo.org/event/26-arc.... Registration is open. We are looking forward to hosting you!
Molecular Biology of Archaea: Life Through the Prism of Archaea
In 1977, Woese and colleagues revealed Archaea as a distinct domain of life. Building on this insight, the discovery of Asgard archaea has strengthened the view that many hallmarks of eukaryotic cell…
meetings.embo.org
January 23, 2026 at 8:15 AM
A reminder to sign up for the 2026 EMBO archaeal meeting im Cambridge: meetings.embo.org/event/26-arc.... Registration is open. We are looking forward to hosting you!
Reposted by Florian Wollweber
🔬🚨New preprint alert! 🚨🔬
We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology
www.biorxiv.org/content/10.6...
#SuperResolution #CellBiology
We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology
www.biorxiv.org/content/10.6...
#SuperResolution #CellBiology
www.biorxiv.org
January 20, 2026 at 4:12 PM
🔬🚨New preprint alert! 🚨🔬
We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology
www.biorxiv.org/content/10.6...
#SuperResolution #CellBiology
We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology
www.biorxiv.org/content/10.6...
#SuperResolution #CellBiology