Florian Wollweber
@florianwollweber.bsky.social
Imaging Asgard archaea and other strange organisms @embl.org Grenoble | 🦠❄️🔬#teamtomo
embl.org/wollweber
embl.org/wollweber
Pinned
Asgard archaea have actin - but what about microtubules? Where do they come from? 🧐 Our new paper www.cell.com/cell/fulltex... by @xujwet.bsky.social & @florianwollweber.bsky.social, in collaboration with the Schleper & Wieczorek labs, describes tiny Asgard microtubules! #TeamTomo #ArchaeaSky 1/6
Asgard archaea are full of surprises, this time we found (tiny) microtubules (snapshots of microtubule evolution?!) - Check out our new paper on the cytoskeleton of Lokiarchaeum ossiferum: www.cell.com/cell/fulltex...
Reposted by Florian Wollweber
We have wondered what a complex archaeal cell might look like ever since 2014. It’s been a long road (and the journey is far from over), but it’s a good time to pause for breath and look. These Asgard archaeal cells are a surprise! And that is the joy of being a cell biologist.
November 7, 2025 at 11:02 AM
We have wondered what a complex archaeal cell might look like ever since 2014. It’s been a long road (and the journey is far from over), but it’s a good time to pause for breath and look. These Asgard archaeal cells are a surprise! And that is the joy of being a cell biologist.
Reposted by Florian Wollweber
An Asgard archaeon with internal membrane compartments
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
November 7, 2025 at 10:44 AM
An Asgard archaeon with internal membrane compartments
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
Reposted by Florian Wollweber
Great collaboration with @brunlabcaulo.bsky.social for resolving the Tad pilus machine structure by cryo-ET. Beautiful work by graduate students James Iarocci and Ryu Williston in our lab.
Preprint:https://www.biorxiv.org/content/10.1101/2025.11.05.686773v1
Preprint:https://www.biorxiv.org/content/10.1101/2025.11.05.686773v1
November 6, 2025 at 3:58 PM
Great collaboration with @brunlabcaulo.bsky.social for resolving the Tad pilus machine structure by cryo-ET. Beautiful work by graduate students James Iarocci and Ryu Williston in our lab.
Preprint:https://www.biorxiv.org/content/10.1101/2025.11.05.686773v1
Preprint:https://www.biorxiv.org/content/10.1101/2025.11.05.686773v1
Reposted by Florian Wollweber
Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...
November 6, 2025 at 7:38 AM
Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...
Reposted by Florian Wollweber
This paper has been a must! Great collaboration with @mkrupovic.bsky.social and @yifanzhou.bsky.social, a N&V by a legend of halophilic archaea tinyurl.com/yc3dcv72, and one picture of one of our expeditions to Dallol making the cover of the November issue of @natmicrobiol.nature.com
rdcu.be/eLtCH
rdcu.be/eLtCH
November 2, 2025 at 5:52 PM
This paper has been a must! Great collaboration with @mkrupovic.bsky.social and @yifanzhou.bsky.social, a N&V by a legend of halophilic archaea tinyurl.com/yc3dcv72, and one picture of one of our expeditions to Dallol making the cover of the November issue of @natmicrobiol.nature.com
rdcu.be/eLtCH
rdcu.be/eLtCH
Thank you so much Martin and everyone @pilhoferlab.bsky.social for 5 years of super fun science and your support - imaging Lokis wouldn’t have been possible anywhere else! 🦠🔬
🥳 Today marks the start of the lab of former postdoc @florianwollweber.bsky.social at @embl.org Grenoble!
www.embl.org/groups/wollw...
Florian-- we will miss you dearly and wish you much success!
With us, Florian applied multiscale imaging 🔬to Asgard archaea ...👇
www.embl.org/groups/wollw...
Florian-- we will miss you dearly and wish you much success!
With us, Florian applied multiscale imaging 🔬to Asgard archaea ...👇
November 2, 2025 at 5:41 PM
Thank you so much Martin and everyone @pilhoferlab.bsky.social for 5 years of super fun science and your support - imaging Lokis wouldn’t have been possible anywhere else! 🦠🔬
Reposted by Florian Wollweber
Exciting day for the lab: our 1rst paper is officially out in @currentbiology.bsky.social 🥳 Wonderful collaboration wt @gautamdey.bsky.social showing how Cryo-ExM achieves consistent immunostaining in diverse diatoms, from the lab and the natural environment 1/n
#ProtistsOnSky
tinyurl.com/2zxaund7
#ProtistsOnSky
tinyurl.com/2zxaund7
October 31, 2025 at 3:27 PM
Exciting day for the lab: our 1rst paper is officially out in @currentbiology.bsky.social 🥳 Wonderful collaboration wt @gautamdey.bsky.social showing how Cryo-ExM achieves consistent immunostaining in diverse diatoms, from the lab and the natural environment 1/n
#ProtistsOnSky
tinyurl.com/2zxaund7
#ProtistsOnSky
tinyurl.com/2zxaund7
Reposted by Florian Wollweber
🚨Our collaboration with @centriolelab.bsky.social & @gautamdey.bsky.social is out today in @cp-cell.bsky.social
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky
www.cell.com/cell/fulltex...
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky
www.cell.com/cell/fulltex...
October 31, 2025 at 2:42 PM
🚨Our collaboration with @centriolelab.bsky.social & @gautamdey.bsky.social is out today in @cp-cell.bsky.social
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky
www.cell.com/cell/fulltex...
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky
www.cell.com/cell/fulltex...
Reposted by Florian Wollweber
How to become a "hot RNA" 🌡️? The answers were kindly provided by hyperthermophilic archaea: rRNA modifications are key! Check out this new publication presenting pan-modification profiling of the epitranscriptome. www.cell.com/cell/fulltex.... (1/3)
Pan-modification profiling facilitates a cross-evolutionary dissection of the thermoregulated ribosomal epitranscriptome
Pan-Mod-seq enables systematic, multi-modification mapping of rRNA across life. Applying
it to diverse organisms reveals that hyperthermophiles dynamically install stabilizing
modifications to support...
www.cell.com
October 27, 2025 at 1:19 PM
How to become a "hot RNA" 🌡️? The answers were kindly provided by hyperthermophilic archaea: rRNA modifications are key! Check out this new publication presenting pan-modification profiling of the epitranscriptome. www.cell.com/cell/fulltex.... (1/3)
Reposted by Florian Wollweber
Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...
October 28, 2025 at 3:07 PM
Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...
Reposted by Florian Wollweber
After exploring Europe’s coastlines, EMBL’s TREC expedition is moving inland! 💧
The new Traversing Ecosystems phase focuses on freshwater habitats – studying how human activity and environmental change affect life at the molecular level.
www.embl.org/news/science...
The new Traversing Ecosystems phase focuses on freshwater habitats – studying how human activity and environmental change affect life at the molecular level.
www.embl.org/news/science...
October 23, 2025 at 9:02 AM
After exploring Europe’s coastlines, EMBL’s TREC expedition is moving inland! 💧
The new Traversing Ecosystems phase focuses on freshwater habitats – studying how human activity and environmental change affect life at the molecular level.
www.embl.org/news/science...
The new Traversing Ecosystems phase focuses on freshwater habitats – studying how human activity and environmental change affect life at the molecular level.
www.embl.org/news/science...
Reposted by Florian Wollweber
Our colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧
Icecream: High-Fidelity Equivariant Cryo-Electron Tomography
Cryo-electron tomography (cryo-ET) visualizes 3D cellular architecture in near-native states. Recent deep-learning methods (CryoCARE, IsoNet, DeepDeWedge, CryoLithe) improve denoising and artifact cor...
www.biorxiv.org
October 23, 2025 at 11:13 AM
Our colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧
Reposted by Florian Wollweber
Baker Lab 2025
The container is our field gear for the research expedition for Uruguay.
sites.utexas.edu/baker-lab/sp...
The container is our field gear for the research expedition for Uruguay.
sites.utexas.edu/baker-lab/sp...
September 18, 2025 at 9:35 PM
Baker Lab 2025
The container is our field gear for the research expedition for Uruguay.
sites.utexas.edu/baker-lab/sp...
The container is our field gear for the research expedition for Uruguay.
sites.utexas.edu/baker-lab/sp...
Reposted by Florian Wollweber
Last Friday (17th), we had an amazing first Young Swiss Tomo meeting in Bern, organized with Elda Bauda, Zanetta Kechagia, and @leonieanton.bsky.social!
Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬.
Huge thanks to the DCI for making the event possible! See you next year! 👋
Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬.
Huge thanks to the DCI for making the event possible! See you next year! 👋
October 19, 2025 at 9:16 AM
Last Friday (17th), we had an amazing first Young Swiss Tomo meeting in Bern, organized with Elda Bauda, Zanetta Kechagia, and @leonieanton.bsky.social!
Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬.
Huge thanks to the DCI for making the event possible! See you next year! 👋
Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬.
Huge thanks to the DCI for making the event possible! See you next year! 👋
Reposted by Florian Wollweber
Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️
www.biorxiv.org/content/10.1...
#teamtomo
www.biorxiv.org/content/10.1...
#teamtomo
October 15, 2025 at 6:26 AM
Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️
www.biorxiv.org/content/10.1...
#teamtomo
www.biorxiv.org/content/10.1...
#teamtomo
Reposted by Florian Wollweber
❓What if you could peek between your tilt series ❓ We are proud to introduce cryoTIGER -- deep-learning based tilt interpolation for enhanced reconstruction in cryo-ET. A great collaboration between Turoňová lab, @becklab.bsky.social, @hummerlab.bsky.social #teamtomo www.nature.com/articles/s42...
cryoTIGER: deep-learning based tilt interpolation generator for enhanced reconstruction in cryo electron tomography - Communications Biology
Deep learning-based interpolation using cryoTIGER enhances angular sampling in cryoET, improving 3D reconstructions, particle localization, and structural recovery without increasing electron dose.
www.nature.com
October 10, 2025 at 10:35 AM
❓What if you could peek between your tilt series ❓ We are proud to introduce cryoTIGER -- deep-learning based tilt interpolation for enhanced reconstruction in cryo-ET. A great collaboration between Turoňová lab, @becklab.bsky.social, @hummerlab.bsky.social #teamtomo www.nature.com/articles/s42...
Reposted by Florian Wollweber
Huge congratulations to Wieczorek Lab PhD student Bin Cai and now-PI Jingwei Xu @xujwet.bsky.social on figuring out the surprisingly complex structure of the A-C linker crosslinking microtubule triplets in Tetrahymena basal body centrioles! www.science.org/doi/10.1126/...
Structure and assembly of the A-C linker connecting microtubule triplets in centrioles
Multiscale cryo-EM of centrioles reveals the structure and polymorphism of the A-C linker connecting microtubule triplets.
www.science.org
October 10, 2025 at 1:14 PM
Huge congratulations to Wieczorek Lab PhD student Bin Cai and now-PI Jingwei Xu @xujwet.bsky.social on figuring out the surprisingly complex structure of the A-C linker crosslinking microtubule triplets in Tetrahymena basal body centrioles! www.science.org/doi/10.1126/...
Reposted by Florian Wollweber
✨Excited that the main project of my PhD is now available as a pre-print on #bioRxiv
Here, we used #CryoET to visualise mitochondrial proteostatic stress and together with SPA #CryoEM shed light into the functional cycle of the Hsp60:10 chaperone system. #TeamTomo
🔗 www.biorxiv.org/content/10.1...
Here, we used #CryoET to visualise mitochondrial proteostatic stress and together with SPA #CryoEM shed light into the functional cycle of the Hsp60:10 chaperone system. #TeamTomo
🔗 www.biorxiv.org/content/10.1...
October 8, 2025 at 3:59 PM
✨Excited that the main project of my PhD is now available as a pre-print on #bioRxiv
Here, we used #CryoET to visualise mitochondrial proteostatic stress and together with SPA #CryoEM shed light into the functional cycle of the Hsp60:10 chaperone system. #TeamTomo
🔗 www.biorxiv.org/content/10.1...
Here, we used #CryoET to visualise mitochondrial proteostatic stress and together with SPA #CryoEM shed light into the functional cycle of the Hsp60:10 chaperone system. #TeamTomo
🔗 www.biorxiv.org/content/10.1...
Reposted by Florian Wollweber
🧐Checkout our preprint revealing the stepwise firing💥 mechanism of a Contractile Injection System @xujwet.bsky.social&@chipericson.bsky.social trapped the complex by structure-guided engineering🧪 in multiple intermediate states and imaged them by multimodal #cryoEM❄️🔬
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
October 8, 2025 at 9:18 PM
🧐Checkout our preprint revealing the stepwise firing💥 mechanism of a Contractile Injection System @xujwet.bsky.social&@chipericson.bsky.social trapped the complex by structure-guided engineering🧪 in multiple intermediate states and imaged them by multimodal #cryoEM❄️🔬
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Florian Wollweber
Congrats🥳 to @mike-wieczorek.bsky.social, Bin Cai, and our former lab member Jingwei Xu @xujwet.bsky.social to their multiscale imaging 🔬 story of the centriolar AC linker #teamtomo
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Structure and assembly of the A-C linker connecting microtubule triplets in centrioles
Multiscale cryo-EM of centrioles reveals the structure and polymorphism of the A-C linker connecting microtubule triplets.
www.science.org
October 8, 2025 at 7:07 PM
Congrats🥳 to @mike-wieczorek.bsky.social, Bin Cai, and our former lab member Jingwei Xu @xujwet.bsky.social to their multiscale imaging 🔬 story of the centriolar AC linker #teamtomo
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Florian Wollweber
Out in Science Advances: Our #cryoEM structure of HFTV1, a virus infecting the halophile #archaea. *First full atomic structure (containing all structural proteins) of any tailed virus!* Congrats and thanks to all co-authors and our fantastic collaborators! www.science.org/doi/10.1126/...
Cryo-EM resolves the structure of the archaeal dsDNA virus HFTV1 from head to tail
This structure of an archaeal tailed virus (arTV) provides detailed insights into arTV assembly and infection mechanisms.
www.science.org
October 6, 2025 at 11:31 AM
Out in Science Advances: Our #cryoEM structure of HFTV1, a virus infecting the halophile #archaea. *First full atomic structure (containing all structural proteins) of any tailed virus!* Congrats and thanks to all co-authors and our fantastic collaborators! www.science.org/doi/10.1126/...
Reposted by Florian Wollweber
Look at this nice cover: beautiful archaella, image made by @sshamphavi.bsky.social !
Thanks also to Marta Rodeiguez for keeping the EM im shape in @ottlab.bsky.social lab!
At @biologyunifreiburg.bsky.social at @uni-freiburg.de @sfb1381.bsky.social @cibss.bsky.social
Thanks also to Marta Rodeiguez for keeping the EM im shape in @ottlab.bsky.social lab!
At @biologyunifreiburg.bsky.social at @uni-freiburg.de @sfb1381.bsky.social @cibss.bsky.social
Some keen Observations in Our October issue!
🦠 Cancer microbiome
🌊 Marine microbes in warming oceans
🌐 broad-range phages
🧬 genetically minimised Salmonella
🔧 Viral infection mechanisms
🧪 Oxaloacetate antiviral defence
and more, here: www.nature.com/nmicrobiol/v...
🦠 Cancer microbiome
🌊 Marine microbes in warming oceans
🌐 broad-range phages
🧬 genetically minimised Salmonella
🔧 Viral infection mechanisms
🧪 Oxaloacetate antiviral defence
and more, here: www.nature.com/nmicrobiol/v...
October 2, 2025 at 8:28 AM
Look at this nice cover: beautiful archaella, image made by @sshamphavi.bsky.social !
Thanks also to Marta Rodeiguez for keeping the EM im shape in @ottlab.bsky.social lab!
At @biologyunifreiburg.bsky.social at @uni-freiburg.de @sfb1381.bsky.social @cibss.bsky.social
Thanks also to Marta Rodeiguez for keeping the EM im shape in @ottlab.bsky.social lab!
At @biologyunifreiburg.bsky.social at @uni-freiburg.de @sfb1381.bsky.social @cibss.bsky.social
Reposted by Florian Wollweber
A New Age of Advanced Volume Microscopy for Protists onlinelibrary.wiley.com/doi/10.1111/... #protists #protistsonsky
October 1, 2025 at 4:02 PM
A New Age of Advanced Volume Microscopy for Protists onlinelibrary.wiley.com/doi/10.1111/... #protists #protistsonsky
Reposted by Florian Wollweber
Dear all, registration for the upcoming EMBO/EMBL Symposium on "the cellular mechanics of symbiosis" is now OPEN 🤩 ! Feel free to register ! www.embl.org/about/info/c...
#EESSymbiosis #ProtistsOnSky #SymbioSky
@hassansalem.bsky.social @berasymbionts.bsky.social @embl.org
#EESSymbiosis #ProtistsOnSky #SymbioSky
@hassansalem.bsky.social @berasymbionts.bsky.social @embl.org
The cellular mechanics of symbiosis: sensing friend from foe
www.embl.org
September 29, 2025 at 8:15 AM
Dear all, registration for the upcoming EMBO/EMBL Symposium on "the cellular mechanics of symbiosis" is now OPEN 🤩 ! Feel free to register ! www.embl.org/about/info/c...
#EESSymbiosis #ProtistsOnSky #SymbioSky
@hassansalem.bsky.social @berasymbionts.bsky.social @embl.org
#EESSymbiosis #ProtistsOnSky #SymbioSky
@hassansalem.bsky.social @berasymbionts.bsky.social @embl.org
Reposted by Florian Wollweber
Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit
www.nexperion.net/semcm2026
www.nexperion.net/semcm2026
September 23, 2025 at 10:42 AM
Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit
www.nexperion.net/semcm2026
www.nexperion.net/semcm2026