Ioannis Tsirkas
@ioannistsirkas.bsky.social
Post doc and manga lover, I will be sharing science-related stuff and manga reads.
Reposted by Ioannis Tsirkas
DNA replication initiation timing is important for maintaining genome integrity | Journal of Bacteriology journals.asm.org/doi/10.1128/...
DNA replication initiation timing is important for maintaining genome integrity | Journal of Bacteriology
The regulation of DNA replication is fundamental to cell growth and cell cycle control.
In eukaryotes, under-initiation or over-initiation leads to genome instability. In
bacteria, it is unclear how c...
journals.asm.org
August 4, 2025 at 10:28 AM
DNA replication initiation timing is important for maintaining genome integrity | Journal of Bacteriology journals.asm.org/doi/10.1128/...
Reposted by Ioannis Tsirkas
Such a nice review for one of our latest research works!
🔬 Stephan Hamperl uncovered how clashes between #gene reading & #DNA copying inside cells drive disease. Their tools reveal new insights into #cancer, ageing & development.
Read more 👉 europa.eu/!H7RtjQ
#FrontierResearch #GeneEditing #Genetics #Oncology @www.helmholtz-munich.de
Read more 👉 europa.eu/!H7RtjQ
#FrontierResearch #GeneEditing #Genetics #Oncology @www.helmholtz-munich.de
Revealing cellular conflict role in disease and development
Understanding the conflicts that happen within our cells could shed new light not only on disease progression, but also on how our bodies...
europa.eu
August 4, 2025 at 12:44 PM
Such a nice review for one of our latest research works!
We look forward to the community's feedback!
Our latest research is online in the @biorxivpreprint.bsky.social! 🧬🎉
🔎 We have developed a novel, single-cell real-time approach to monitor R-loop generated TRCs, and we have shown R-loop-mediated, replisome-controlled transcription repression.
www.biorxiv.org/cgi/content/...
🔎 We have developed a novel, single-cell real-time approach to monitor R-loop generated TRCs, and we have shown R-loop-mediated, replisome-controlled transcription repression.
www.biorxiv.org/cgi/content/...
Replisome progression regulates R-loop mediated transcriptional repression
Maintaining cellular proliferation necessitates the synchronized activity of diverse molecular machineries operating in parallel on the genome. Wide-spread transcription and R-loop formation can inter...
www.biorxiv.org
August 4, 2025 at 2:35 PM
We look forward to the community's feedback!
Reposted by Ioannis Tsirkas
The BLS did exactly what it's supposed to do: provide accurate, nonpartisan data. When the numbers showed Trump's "economic boom" was actually a bust (33k jobs vs claimed 291k), he fired the messenger. After all, when it rains too much, true leaders fire the meteorologists.
We’re officially in Donald Trump’s mad king era
All the books that are fit to cook.
www.motherjones.com
August 2, 2025 at 3:31 PM
The BLS did exactly what it's supposed to do: provide accurate, nonpartisan data. When the numbers showed Trump's "economic boom" was actually a bust (33k jobs vs claimed 291k), he fired the messenger. After all, when it rains too much, true leaders fire the meteorologists.
Reposted by Ioannis Tsirkas
I don't like the person I become when designing primers
July 19, 2025 at 6:54 PM
I don't like the person I become when designing primers
Reposted by Ioannis Tsirkas
I am very excited to share our latest work where we describe a new method to profile genome-wide chromatin transitions over time in single cells. Great collaborative effort with the van Oudenaarden group @hubrechtinstitute.bsky.social @oncodeinstitute.bsky.social www.nature.com/articles/s41....
Retrospective and multifactorial single-cell profiling reveals sequential chromatin reorganization during X inactivation - Nature Cell Biology
Kefalopoulou, Rullens et al. develop Dam&ChIC to assay chromatin state at two different time points in the same cell. The method was used to study the reorganization of LADs during cell division a...
www.nature.com
July 10, 2025 at 2:36 PM
I am very excited to share our latest work where we describe a new method to profile genome-wide chromatin transitions over time in single cells. Great collaborative effort with the van Oudenaarden group @hubrechtinstitute.bsky.social @oncodeinstitute.bsky.social www.nature.com/articles/s41....
Reposted by Ioannis Tsirkas
RIF1 orchestrates a multi-step restoration of post-replicative H3K9me3 https://www.biorxiv.org/content/10.1101/2025.07.14.664777v1
July 15, 2025 at 8:30 AM
RIF1 orchestrates a multi-step restoration of post-replicative H3K9me3 https://www.biorxiv.org/content/10.1101/2025.07.14.664777v1
Reposted by Ioannis Tsirkas
🎤 Apply to speak @our TALENT FORUM by July 31!
Showcase YOUR research in Epigenetics & NucleicAcids @www.helmholtz-munich.de & connect with leading scientists
🧳Travel+accommodation covered if selected!
📝 Info+how to apply: lnkd.in/drkRvz7N
#postdoc #career #groupleader #HelmholtzMunich #research
Showcase YOUR research in Epigenetics & NucleicAcids @www.helmholtz-munich.de & connect with leading scientists
🧳Travel+accommodation covered if selected!
📝 Info+how to apply: lnkd.in/drkRvz7N
#postdoc #career #groupleader #HelmholtzMunich #research
July 15, 2025 at 8:24 AM
🎤 Apply to speak @our TALENT FORUM by July 31!
Showcase YOUR research in Epigenetics & NucleicAcids @www.helmholtz-munich.de & connect with leading scientists
🧳Travel+accommodation covered if selected!
📝 Info+how to apply: lnkd.in/drkRvz7N
#postdoc #career #groupleader #HelmholtzMunich #research
Showcase YOUR research in Epigenetics & NucleicAcids @www.helmholtz-munich.de & connect with leading scientists
🧳Travel+accommodation covered if selected!
📝 Info+how to apply: lnkd.in/drkRvz7N
#postdoc #career #groupleader #HelmholtzMunich #research
Great week for #manga readers!
#WildStrawberry ch45
#Yattara ch18
#DramaQueen ch23
#Centuria ch56
#Dandadan ch198
#Chainsawman ch206
#Bugego ch10
#MärchenCrown ch11
#TheGodofTime ch5
#AsurasVerdict ch14
Especially the last one was so good! Here are a few artworks.
#WildStrawberry ch45
#Yattara ch18
#DramaQueen ch23
#Centuria ch56
#Dandadan ch198
#Chainsawman ch206
#Bugego ch10
#MärchenCrown ch11
#TheGodofTime ch5
#AsurasVerdict ch14
Especially the last one was so good! Here are a few artworks.
June 19, 2025 at 12:59 PM
Great week for #manga readers!
#WildStrawberry ch45
#Yattara ch18
#DramaQueen ch23
#Centuria ch56
#Dandadan ch198
#Chainsawman ch206
#Bugego ch10
#MärchenCrown ch11
#TheGodofTime ch5
#AsurasVerdict ch14
Especially the last one was so good! Here are a few artworks.
#WildStrawberry ch45
#Yattara ch18
#DramaQueen ch23
#Centuria ch56
#Dandadan ch198
#Chainsawman ch206
#Bugego ch10
#MärchenCrown ch11
#TheGodofTime ch5
#AsurasVerdict ch14
Especially the last one was so good! Here are a few artworks.
Reposted by Ioannis Tsirkas
A Multimodal Adaptive Optical Microscope For In Vivo Imaging from Molecules to Organisms https://www.biorxiv.org/content/10.1101/2025.06.02.657494v1
June 16, 2025 at 8:30 AM
A Multimodal Adaptive Optical Microscope For In Vivo Imaging from Molecules to Organisms https://www.biorxiv.org/content/10.1101/2025.06.02.657494v1
June 13, 2025 at 5:43 PM
Reposted by Ioannis Tsirkas
Excited our paper is out! G-quadruplex (G4) unwinding via an intricate G-loop assembly and disassembly mechanism maintains genome integrity. Pioneered by Koichi Sato with great collaborators Jing Lyu and @simonelsasser.bsky.social Check it out: www.science.org/doi/10.1126/science.adr0493 🧵(1/4)
RNA transcripts regulate G-quadruplex landscapes through G-loop formation
G-quadruplexes (G4s) are prevalent DNA structures that regulate transcription but also threaten genome stability. How G4 dynamics are controlled remains poorly understood. Here, we report that RNA tra...
www.science.org
June 13, 2025 at 9:33 AM
Excited our paper is out! G-quadruplex (G4) unwinding via an intricate G-loop assembly and disassembly mechanism maintains genome integrity. Pioneered by Koichi Sato with great collaborators Jing Lyu and @simonelsasser.bsky.social Check it out: www.science.org/doi/10.1126/science.adr0493 🧵(1/4)
#spoileralert
#dandadan #manga nailed it this time, one of its coolest #artworks! Remember, this is a weekly #manga!
#dandadan #manga nailed it this time, one of its coolest #artworks! Remember, this is a weekly #manga!
June 9, 2025 at 7:32 PM
#spoileralert
#dandadan #manga nailed it this time, one of its coolest #artworks! Remember, this is a weekly #manga!
#dandadan #manga nailed it this time, one of its coolest #artworks! Remember, this is a weekly #manga!
This was a gift from a colleague, and it looks breathtaking. Done within one day (1000 pieces, around 12-15 hours)
June 8, 2025 at 7:22 PM
This was a gift from a colleague, and it looks breathtaking. Done within one day (1000 pieces, around 12-15 hours)
Reposted by Ioannis Tsirkas
A rather remarkable retraction for a paper where apparently no authors contributed! I guess these #papermill authors were a bit too honest... #fraud
@elsevierconnect.bsky.social
doi.org/10.1016/j.ij...
@elsevierconnect.bsky.social
doi.org/10.1016/j.ij...
May 27, 2025 at 3:18 PM
A rather remarkable retraction for a paper where apparently no authors contributed! I guess these #papermill authors were a bit too honest... #fraud
@elsevierconnect.bsky.social
doi.org/10.1016/j.ij...
@elsevierconnect.bsky.social
doi.org/10.1016/j.ij...
Reposted by Ioannis Tsirkas
CST complex blocks end resection by BLM-DNA2 or EXO1, therefore promoting non-homologous endjoining and inhibiting repair templated DNA repair.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
CTC1-STN1-TEN1 controls DNA break repair pathway choice via DNA end resection blockade
Antagonistic activities of the 53BP1 axis and the tumor suppressor BRCA1-BARD1 determine whether DNA double-strand breaks (DSBs) are repaired by end joining or homologous recombination. We show that t...
www.science.org
May 26, 2025 at 1:26 AM
CST complex blocks end resection by BLM-DNA2 or EXO1, therefore promoting non-homologous endjoining and inhibiting repair templated DNA repair.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Ioannis Tsirkas
Delighted to share that the results of my main postdoc project are now published in @nature.com
🔗 doi.org/10.1038/s415...
🔗 doi.org/10.1038/s415...
Multigenerational cell tracking of DNA replication and heritable DNA damage - Nature
A multigenerational single-cell tracking approach provides a framework to dissect phenotypic plasticity at the single-cell level, offering insights into cellular processes that may resemble early even...
doi.org
May 21, 2025 at 3:27 PM
Delighted to share that the results of my main postdoc project are now published in @nature.com
🔗 doi.org/10.1038/s415...
🔗 doi.org/10.1038/s415...
Reposted by Ioannis Tsirkas
Grab a pint & dive into science! 🍻
Pint of Science is back in Munich:
🗓️ May 19–21, 2025
🕢 19:30–22:00
#HelmholtzMunich researchers share big ideas – from AI to antibodies.
🎟️ Info & tickets: t1p.de/lvpuv
#pint25 #pint25de
Pint of Science is back in Munich:
🗓️ May 19–21, 2025
🕢 19:30–22:00
#HelmholtzMunich researchers share big ideas – from AI to antibodies.
🎟️ Info & tickets: t1p.de/lvpuv
#pint25 #pint25de
Pint of Science Munich
Explore Pint of Science Deutschland events in Munich
pintofscience.de
May 14, 2025 at 11:40 AM
Grab a pint & dive into science! 🍻
Pint of Science is back in Munich:
🗓️ May 19–21, 2025
🕢 19:30–22:00
#HelmholtzMunich researchers share big ideas – from AI to antibodies.
🎟️ Info & tickets: t1p.de/lvpuv
#pint25 #pint25de
Pint of Science is back in Munich:
🗓️ May 19–21, 2025
🕢 19:30–22:00
#HelmholtzMunich researchers share big ideas – from AI to antibodies.
🎟️ Info & tickets: t1p.de/lvpuv
#pint25 #pint25de
May 12, 2025 at 7:53 PM
Reposted by Ioannis Tsirkas
Excited to share our latest from my postdoc at @mskcancercenter.bsky.social in the Sfeir Lab, in collaboration with the Simon Powell Lab! We uncovered a novel genome repair mechanism in human cells: RNA-templated DNA double-strand break repair (RT-DSBR). (1/5)
May 12, 2025 at 5:49 PM
Excited to share our latest from my postdoc at @mskcancercenter.bsky.social in the Sfeir Lab, in collaboration with the Simon Powell Lab! We uncovered a novel genome repair mechanism in human cells: RNA-templated DNA double-strand break repair (RT-DSBR). (1/5)
Reposted by Ioannis Tsirkas
I used to have nightmares about getting a pet and forgetting to feed it.
I have not had a single one of those dreams since getting Heater because there is not a chance in Hell she will let me forget breakfast.
I have not had a single one of those dreams since getting Heater because there is not a chance in Hell she will let me forget breakfast.
May 7, 2025 at 1:43 PM
I used to have nightmares about getting a pet and forgetting to feed it.
I have not had a single one of those dreams since getting Heater because there is not a chance in Hell she will let me forget breakfast.
I have not had a single one of those dreams since getting Heater because there is not a chance in Hell she will let me forget breakfast.
Reposted by Ioannis Tsirkas
I just woke up from a nightmare where I was giving a guest lecture to an undergrad cell biology class and none of them knew the central dogma or what DNA or RNA were. I spent an hour teaching that instead. They didn’t understand why they needed to know about DNA for cell biology. 🫠😫😱
homer simpson is laying in bed under a blue blanket .
Alt: homer simpson is laying in bed under a blue blanket waking up from a nightmare
media.tenor.com
May 7, 2025 at 12:17 PM
I just woke up from a nightmare where I was giving a guest lecture to an undergrad cell biology class and none of them knew the central dogma or what DNA or RNA were. I spent an hour teaching that instead. They didn’t understand why they needed to know about DNA for cell biology. 🫠😫😱
Reposted by Ioannis Tsirkas
What governs where DNA replication begins in human cells? Here, we show that origins of replication in human DNA are epigenetically specified and that crucially, this epigenetic mark is required for both DNA replication and cell proliferation! @narjournal.bsky.social academic.oup.com/nar/article/...
May 6, 2025 at 9:39 PM
What governs where DNA replication begins in human cells? Here, we show that origins of replication in human DNA are epigenetically specified and that crucially, this epigenetic mark is required for both DNA replication and cell proliferation! @narjournal.bsky.social academic.oup.com/nar/article/...
Reposted by Ioannis Tsirkas
Fluorescence recovery in the Super-Resolution regime reveals subcompartments of 53BP1 foci https://www.biorxiv.org/content/10.1101/2025.05.07.652606v1
May 7, 2025 at 4:30 PM
Fluorescence recovery in the Super-Resolution regime reveals subcompartments of 53BP1 foci https://www.biorxiv.org/content/10.1101/2025.05.07.652606v1