Harry M. Williams
@harrywilliams.bsky.social
Structural biologist currently working on microsecond time-resolved cryo-EM | Currently based in Lausanne at EPFL 🇨🇭
Pinned
Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification https://www.biorxiv.org/content/10.1101/2025.09.14.676144v1
❄️ NEW PRE-PRINT ❄️ Happy to see our latest work online. Here we present a super simple solution to the preferred orientation problem in single particle cryo-EM: the use of ultrasonic excitation during vitrification! Details in the 🧵 below... #cryoEM #structuralbiology
Reposted by Harry M. Williams
We are pleased to announce Doppio version 1.4! Available here www.ccpem.ac.uk/software/dow...
Downloads – CCP-EM
www.ccpem.ac.uk
November 4, 2025 at 5:38 PM
We are pleased to announce Doppio version 1.4! Available here www.ccpem.ac.uk/software/dow...
Reposted by Harry M. Williams
Our new preprint is out 🥳🥳🥳
Henipaviruses, like Nipah and Hendra, package their genomes inside helical shells built by thousands of nucleoproteins. These nucleocapsids are essential to protect the viral RNA, but how do they ever let the polymerase in to read the sequence?
👇
Henipaviruses, like Nipah and Hendra, package their genomes inside helical shells built by thousands of nucleoproteins. These nucleocapsids are essential to protect the viral RNA, but how do they ever let the polymerase in to read the sequence?
👇
November 3, 2025 at 12:26 PM
Our new preprint is out 🥳🥳🥳
Henipaviruses, like Nipah and Hendra, package their genomes inside helical shells built by thousands of nucleoproteins. These nucleocapsids are essential to protect the viral RNA, but how do they ever let the polymerase in to read the sequence?
👇
Henipaviruses, like Nipah and Hendra, package their genomes inside helical shells built by thousands of nucleoproteins. These nucleocapsids are essential to protect the viral RNA, but how do they ever let the polymerase in to read the sequence?
👇
Reposted by Harry M. Williams
warwick.ac.uk/fac/cross_fa...
I am advertising for a PhD position in my group. We'll be investigating how pathogenic RNA viruses replicate in the nucleus. The project will teach cryoEM/ET, virus culture, and in vitro assays.
If you're interested reach out and I'd be happy to have a chat.
I am advertising for a PhD position in my group. We'll be investigating how pathogenic RNA viruses replicate in the nucleus. The project will teach cryoEM/ET, virus culture, and in vitro assays.
If you're interested reach out and I'd be happy to have a chat.
Dr Jeremy Keown
Dr Jeremy Keown
warwick.ac.uk
October 24, 2025 at 7:58 AM
warwick.ac.uk/fac/cross_fa...
I am advertising for a PhD position in my group. We'll be investigating how pathogenic RNA viruses replicate in the nucleus. The project will teach cryoEM/ET, virus culture, and in vitro assays.
If you're interested reach out and I'd be happy to have a chat.
I am advertising for a PhD position in my group. We'll be investigating how pathogenic RNA viruses replicate in the nucleus. The project will teach cryoEM/ET, virus culture, and in vitro assays.
If you're interested reach out and I'd be happy to have a chat.
Reposted by Harry M. Williams
Finally, just about 2 years after finishing the postdoc the last part of my work is finally online! We determined the structure of HSV-1 Origin Binding Protein, the viral initiator of DNA replication, by cryo-EM 🧪🧬🧶🧵 #cryoEM #structuralbiology
October 23, 2025 at 6:37 AM
Finally, just about 2 years after finishing the postdoc the last part of my work is finally online! We determined the structure of HSV-1 Origin Binding Protein, the viral initiator of DNA replication, by cryo-EM 🧪🧬🧶🧵 #cryoEM #structuralbiology
Reposted by Harry M. Williams
Chris Russo and Anastasiia Gusach have developed a new method to prepare #cryoEM samples that avoids protein damage during freezing
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
October 22, 2025 at 4:41 PM
Chris Russo and Anastasiia Gusach have developed a new method to prepare #cryoEM samples that avoids protein damage during freezing
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...
#LMBResearch
Reposted by Harry M. Williams
An efficient eukaryotic cell-free expression and correlative cryo-electron tomography platform for structural biology of macromolecular complexes www.biorxiv.org/content/10.1101/2025.10.18.683253v1 #cryoem
October 21, 2025 at 7:10 AM
An efficient eukaryotic cell-free expression and correlative cryo-electron tomography platform for structural biology of macromolecular complexes www.biorxiv.org/content/10.1101/2025.10.18.683253v1 #cryoem
The EPFL/UNIL sheep appear to be thoroughly enjoying the slow move into autumn.
October 7, 2025 at 3:06 PM
The EPFL/UNIL sheep appear to be thoroughly enjoying the slow move into autumn.
This is very cool!
Check out our new preprint on an integrated pipeline combining in situ #cryo-ET with MALDI #MSImaging for single-cell identification and classification from previously analysed EM-grids.
Link: www.biorxiv.org/content/10.1...
Link: www.biorxiv.org/content/10.1...
October 5, 2025 at 8:19 PM
This is very cool!
The Starbucks people know me by name, I think this counts as settling in a new place, right?
October 4, 2025 at 10:31 AM
The Starbucks people know me by name, I think this counts as settling in a new place, right?
Reposted by Harry M. Williams
Today, @rcsbpdb.bsky.social introduces Q-score in EM validation reports. Q-score assesses model-map fit. A new percentile slider will compare an entry’s average Q-score to the full archive and a resolution-matched subset. This would help judge whether the fit is typical for the reported resolution.
Introducing the first 3DEM Model-Map percentile slider to the wwPDB validation report
Introducing the latest wwPDB Percentile slider based on the Q-score model-map validation metric
www.rcsb.org
October 1, 2025 at 12:25 AM
Today, @rcsbpdb.bsky.social introduces Q-score in EM validation reports. Q-score assesses model-map fit. A new percentile slider will compare an entry’s average Q-score to the full archive and a resolution-matched subset. This would help judge whether the fit is typical for the reported resolution.
Reposted by Harry M. Williams
Assistant/Associate Professor in Structural Biology, Biophysics or Biological Imaging
www.biozentrum.unibas.ch/open-positio...
#biozentrum #University #Basel #Professor #Structural #Biology #Biophysics #Biological #Imaging
www.biozentrum.unibas.ch/open-positio...
#biozentrum #University #Basel #Professor #Structural #Biology #Biophysics #Biological #Imaging
September 29, 2025 at 2:58 PM
Assistant/Associate Professor in Structural Biology, Biophysics or Biological Imaging
www.biozentrum.unibas.ch/open-positio...
#biozentrum #University #Basel #Professor #Structural #Biology #Biophysics #Biological #Imaging
www.biozentrum.unibas.ch/open-positio...
#biozentrum #University #Basel #Professor #Structural #Biology #Biophysics #Biological #Imaging
Reposted by Harry M. Williams
I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪
September 23, 2025 at 5:08 PM
I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪
Reposted by Harry M. Williams
We have a preprint of a book chapter for you: Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles.
We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography
zenodo.org/records/1717...
We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography
zenodo.org/records/1717...
Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles
zenodo.org
September 22, 2025 at 12:36 PM
We have a preprint of a book chapter for you: Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles.
We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography
zenodo.org/records/1717...
We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography
zenodo.org/records/1717...
❄️ NEW PRE-PRINT ❄️ Happy to see our latest work online. Here we present a super simple solution to the preferred orientation problem in single particle cryo-EM: the use of ultrasonic excitation during vitrification! Details in the 🧵 below... #cryoEM #structuralbiology
Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification https://www.biorxiv.org/content/10.1101/2025.09.14.676144v1
September 18, 2025 at 8:04 AM
❄️ NEW PRE-PRINT ❄️ Happy to see our latest work online. Here we present a super simple solution to the preferred orientation problem in single particle cryo-EM: the use of ultrasonic excitation during vitrification! Details in the 🧵 below... #cryoEM #structuralbiology
Reposted by Harry M. Williams
🌈 A double rainbow over CSSB on Monday! Thanks to @carobarisch.bsky.social for the amazing photo.
@topf-lab.bsky.social @bosse-lab.bsky.social @jankosinski.bsky.social @landaulabcssb.bsky.social @uni-hamburg.de @leibnizliv.bsky.social
@topf-lab.bsky.social @bosse-lab.bsky.social @jankosinski.bsky.social @landaulabcssb.bsky.social @uni-hamburg.de @leibnizliv.bsky.social
September 17, 2025 at 12:52 PM
🌈 A double rainbow over CSSB on Monday! Thanks to @carobarisch.bsky.social for the amazing photo.
@topf-lab.bsky.social @bosse-lab.bsky.social @jankosinski.bsky.social @landaulabcssb.bsky.social @uni-hamburg.de @leibnizliv.bsky.social
@topf-lab.bsky.social @bosse-lab.bsky.social @jankosinski.bsky.social @landaulabcssb.bsky.social @uni-hamburg.de @leibnizliv.bsky.social
Reposted by Harry M. Williams
Confidence-guided cryo-EM map optimisation with LocScale-2.0 www.biorxiv.org/content/10.1101/2025.09.11.674726v1 #cryoem
September 15, 2025 at 7:11 AM
Confidence-guided cryo-EM map optimisation with LocScale-2.0 www.biorxiv.org/content/10.1101/2025.09.11.674726v1 #cryoem
Reposted by Harry M. Williams
CSSB's Uetrecht group has revealed new insights into the mechanisms of human corona viruses. Their findings were published today in @natcomms.nature.com
@uniluebeck.bsky.social @leibnizliv.bsky.social
Find out more: t1p.de/7ij0e
@uniluebeck.bsky.social @leibnizliv.bsky.social
Find out more: t1p.de/7ij0e
September 9, 2025 at 12:58 PM
CSSB's Uetrecht group has revealed new insights into the mechanisms of human corona viruses. Their findings were published today in @natcomms.nature.com
@uniluebeck.bsky.social @leibnizliv.bsky.social
Find out more: t1p.de/7ij0e
@uniluebeck.bsky.social @leibnizliv.bsky.social
Find out more: t1p.de/7ij0e
You cannot beat a Saturday afternoon plane spotting at Geneva. The smell of Jet A-1 really clears the mind.
September 6, 2025 at 6:22 PM
You cannot beat a Saturday afternoon plane spotting at Geneva. The smell of Jet A-1 really clears the mind.
Reposted by Harry M. Williams
RNAi is a powerful tool for mosquito control, but delivery remains difficult.
Could symbiotic bacteria be the key? Mixed results so far highlight the complexity and the gaps in our understanding of RNAi, bacterial delivery, and host–symbiont interactions.
Read more in our recent review👇
Could symbiotic bacteria be the key? Mixed results so far highlight the complexity and the gaps in our understanding of RNAi, bacterial delivery, and host–symbiont interactions.
Read more in our recent review👇
August 25, 2025 at 12:59 PM
RNAi is a powerful tool for mosquito control, but delivery remains difficult.
Could symbiotic bacteria be the key? Mixed results so far highlight the complexity and the gaps in our understanding of RNAi, bacterial delivery, and host–symbiont interactions.
Read more in our recent review👇
Could symbiotic bacteria be the key? Mixed results so far highlight the complexity and the gaps in our understanding of RNAi, bacterial delivery, and host–symbiont interactions.
Read more in our recent review👇
Reposted by Harry M. Williams
We‘re looking for a (Full time) PostDoc at the Institute for Virology at Marburg University to help us with our project about the development of Ebola Virus antivirals. Please apply especially of you are interested in or experienced with BSL4 work: stellenangebote.uni-marburg.de/jobposting/6...
Research Assistant (Postdoc )
stellenangebote.uni-marburg.de
August 18, 2025 at 7:09 PM
We‘re looking for a (Full time) PostDoc at the Institute for Virology at Marburg University to help us with our project about the development of Ebola Virus antivirals. Please apply especially of you are interested in or experienced with BSL4 work: stellenangebote.uni-marburg.de/jobposting/6...
Nice work, Jeremy et al!
Very pleased to have our new work out where we perform a structure/function study on the bornavirus polymerase complex with fantastic coauthors. Have a read www.nature.com/articles/s41... @warwicklifesci.bsky.social @loiccarrique.bsky.social @franziskaguenl.bsky.social
The structure of the mammalian bornavirus polymerase complex - Nature Communications
Borna disease virus 1 replicates and transcribes its negative sense RNA genome in the nucleus of infected cells. Here, the authors present the cryoEM structures of the large polymerase protein in comp...
www.nature.com
August 13, 2025 at 9:48 PM
Nice work, Jeremy et al!
Why is it that we refer to bunyaviral endonucleases as being "cap-dependent"? It's an endonuclease, it will degrade all and any RNA with the correct sequence. Metal-dependent? Sure/probably. But cap-dependent? Highly questionable. [https://journals.asm.org/doi/full/10.1128/aac.00186-25]
A cap-dependent endonuclease inhibitor acts as a potent antiviral agent against La Crosse virus infection | Antimicrobial Agents and Chemotherapy
La Crosse virus (LACV), an arthropod-borne virus, is classified under the genus Orthobunyavirus within the family Peribunyaviridae, order Elliovirales, and class Bunyaviricetes (1). LACV was first iso...
journals.asm.org
August 13, 2025 at 1:52 PM
Why is it that we refer to bunyaviral endonucleases as being "cap-dependent"? It's an endonuclease, it will degrade all and any RNA with the correct sequence. Metal-dependent? Sure/probably. But cap-dependent? Highly questionable. [https://journals.asm.org/doi/full/10.1128/aac.00186-25]
Please make it stop.
Discover the latest updates on viral classification in the ICTV taxonomy summaries collection published in Journal of General Virology: bit.ly/4fBCVPK #JGV #ICTV
ICTV Virus Taxonomy Summaries | Microbiology Society
ICTV Virus Taxonomy Summaries are published by the International Committee on Taxonomy of Viruses (ICTV) annually in Journal of General Virology and provide a complete and authoritative record of all…
www.microbiologyresearch.org
August 12, 2025 at 9:25 AM
Please make it stop.
Apoferritin of the RNA world? Very cool.
Not my area at all, but how cool are these cryoEM structures of purely RNA-based assemblies!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
August 7, 2025 at 7:06 AM
Apoferritin of the RNA world? Very cool.
Reposted by Harry M. Williams
structure prediction is not science if not experimentally validated. period
Predicted structures from the AFDB are poor substitutes for experimental structures when training inverse folding NNs. Here, authors show that this is because they are "too perfect", and passing them through a custom-trained AFDB-to-PDB NN helps fix this
threadreaderapp.com/thread/19384...
threadreaderapp.com/thread/19384...
June 27, 2025 at 9:53 PM
structure prediction is not science if not experimentally validated. period