Charlie Allen
@charlieallen.bsky.social
she/they | tech in the Buchwalter Lab at UCSF studying the nuclear periphery | California Community College alum
Pinned
Charlie Allen
@charlieallen.bsky.social
· Jul 23
Another paper bluetorial! Today: how does the spatial location of genes influence their function? (1/n) www.nature.com/articles/s41...
The nuclear periphery confers repression on H3K9me2-marked genes and transposons to shape cell fate - Nature Cell Biology
Marin et al. report the role of lamin proteins and the lamin B receptor (LBR) in chromatin positioning at the nuclear periphery. Knockout of all lamins and LBR in mouse embryonic stem cells leads to h...
www.nature.com
I'm happy people can read the final version of this paper! See what happens when we detach heterochromatin from the nuclear periphery in mouse stem cells:
I’m sure the NYT would apply this same standard to something like someone alleging misconduct at a pediatric gender clinic, right?
I am not defending Landon’s relationship with Epstein. In fact, the NYT didn’t even try to defend it. He left the paper. What I’m saying is that a piece of investigative journalism that exposes a secret, criminal act such as statutory rape requires far more than what is in these emails.
November 13, 2025 at 7:55 PM
I’m sure the NYT would apply this same standard to something like someone alleging misconduct at a pediatric gender clinic, right?
Reposted by Charlie Allen
“Transgender people are more than just a political issue, and we're in danger. We need all the help we can get from friends, neighbors, politicians, and school administrators.. trans people are increasingly discriminated against, excluded, and treated without respect or dignity…
Help us.”
Help us.”
For @teenvogue.com I wrote about the death of trans athlete Lia Smith and how trans youth kind themselves with central pillars of their lives being constantly ripped away from them because of politics. www.teenvogue.com/story/politi...
Politicizing Transgender Athletes Is Dangerous. Lia Smith Deserved Better.
"Let her passing be an inflection point for how you think about trans rights and trans people more generally."
www.teenvogue.com
November 9, 2025 at 12:53 PM
“Transgender people are more than just a political issue, and we're in danger. We need all the help we can get from friends, neighbors, politicians, and school administrators.. trans people are increasingly discriminated against, excluded, and treated without respect or dignity…
Help us.”
Help us.”
Reposted by Charlie Allen
i stopped paying attention to the bbc after they published a conspiracy theory about trans women raping lesbians.
their 1 source was a sex predator who wrote a manifesto stating trans men should be correctively raped and trans women should be lynched
they still have not retracted or apologised
their 1 source was a sex predator who wrote a manifesto stating trans men should be correctively raped and trans women should be lynched
they still have not retracted or apologised
It’s so difficult to say what “the news” is these days: newspapers and front pages seem largely irrelevant, the BBC is ignored or castigated by so many, and social media is so siloed that “current affairs” seems to exist in multiple separate iterations
I'm guessing the BBC 'scandal' has been big on X this week as this was the first I had heard of it...
November 9, 2025 at 11:02 PM
i stopped paying attention to the bbc after they published a conspiracy theory about trans women raping lesbians.
their 1 source was a sex predator who wrote a manifesto stating trans men should be correctively raped and trans women should be lynched
they still have not retracted or apologised
their 1 source was a sex predator who wrote a manifesto stating trans men should be correctively raped and trans women should be lynched
they still have not retracted or apologised
Reposted by Charlie Allen
Honestly terrifying that the BBC can run an unhinged story about lesbians being forced to have sex with trans women, with one of the main sources being a rapist who responded to the story's publication by calling for trans people to all be hung, and the Official Consensus is they are too pro-trans
November 10, 2025 at 11:51 AM
Honestly terrifying that the BBC can run an unhinged story about lesbians being forced to have sex with trans women, with one of the main sources being a rapist who responded to the story's publication by calling for trans people to all be hung, and the Official Consensus is they are too pro-trans
would love to live somewhere where transit agencies respected people’s time
November 9, 2025 at 7:38 PM
would love to live somewhere where transit agencies respected people’s time
we gotta come up with a good alternative to western blots
November 9, 2025 at 2:05 AM
we gotta come up with a good alternative to western blots
Reposted by Charlie Allen
For @teenvogue.com I wrote about the death of trans athlete Lia Smith and how trans youth kind themselves with central pillars of their lives being constantly ripped away from them because of politics. www.teenvogue.com/story/politi...
Politicizing Transgender Athletes Is Dangerous. Lia Smith Deserved Better.
"Let her passing be an inflection point for how you think about trans rights and trans people more generally."
www.teenvogue.com
November 9, 2025 at 1:23 AM
For @teenvogue.com I wrote about the death of trans athlete Lia Smith and how trans youth kind themselves with central pillars of their lives being constantly ripped away from them because of politics. www.teenvogue.com/story/politi...
would appreciate if my union would not send me political spam texts addressed to my dead mom
November 4, 2025 at 1:54 AM
would appreciate if my union would not send me political spam texts addressed to my dead mom
Reposted by Charlie Allen
1. This week, news emerged that former trans athlete, student at Middlebury, and advocate for trans rights, Lia Smith, was found dead.
Her death was ruled a suicide.
But I argue that it's not only a suicide. Every trans suicide is a murder by those in power.
Subscribe to support my journalism.
Her death was ruled a suicide.
But I argue that it's not only a suicide. Every trans suicide is a murder by those in power.
Subscribe to support my journalism.
Every Trans Suicide Is A Murder By Those In Power
News came this week that transgender athlete and student Lia Smith took her life at just 21 years of age.
www.erininthemorning.com
October 29, 2025 at 4:19 PM
1. This week, news emerged that former trans athlete, student at Middlebury, and advocate for trans rights, Lia Smith, was found dead.
Her death was ruled a suicide.
But I argue that it's not only a suicide. Every trans suicide is a murder by those in power.
Subscribe to support my journalism.
Her death was ruled a suicide.
But I argue that it's not only a suicide. Every trans suicide is a murder by those in power.
Subscribe to support my journalism.
maybe there’s just no place in science for me
November 1, 2025 at 1:05 AM
maybe there’s just no place in science for me
Reposted by Charlie Allen
when trans people take HRT to feel normal: “look at these depraved sex freaks performing their sick fetish in public, we should bully them out of existence”
when cis ppl take HRT so they can fuck real good:
when cis ppl take HRT so they can fuck real good:
October 22, 2025 at 6:14 PM
when trans people take HRT to feel normal: “look at these depraved sex freaks performing their sick fetish in public, we should bully them out of existence”
when cis ppl take HRT so they can fuck real good:
when cis ppl take HRT so they can fuck real good:
might as well do some last minute phd applications this cycle
October 20, 2025 at 11:11 PM
might as well do some last minute phd applications this cycle
Reposted by Charlie Allen
I'm so glad you said this, because about 30 seconds ago, I published a newsletter piece detailing how conservatives have dump-trucked through all of the previously established common sense, nuanced, compromise positions on trans rights to get here: burns-notice.ghost.io/what-would-t...
September 29, 2025 at 4:03 PM
I'm so glad you said this, because about 30 seconds ago, I published a newsletter piece detailing how conservatives have dump-trucked through all of the previously established common sense, nuanced, compromise positions on trans rights to get here: burns-notice.ghost.io/what-would-t...
strongest earthquake i can remember in a long time
September 22, 2025 at 10:07 AM
strongest earthquake i can remember in a long time
Reposted by Charlie Allen
As usual with this issue, Harris is calling for a compromise that already exists. Every sporting body has standards for how long athletes must be on hormones before they can compete as women.
The problem isn’t that we’re ignoring ‘reasonable concerns,’ the problem is that republicans are lying.
The problem isn’t that we’re ignoring ‘reasonable concerns,’ the problem is that republicans are lying.
September 20, 2025 at 8:10 AM
As usual with this issue, Harris is calling for a compromise that already exists. Every sporting body has standards for how long athletes must be on hormones before they can compete as women.
The problem isn’t that we’re ignoring ‘reasonable concerns,’ the problem is that republicans are lying.
The problem isn’t that we’re ignoring ‘reasonable concerns,’ the problem is that republicans are lying.
requiring applicants to not only manually re-enter most of their transcript but also track down a course catalog to copy and paste course descriptions for dozens of courses from years to decades ago is absurd
September 4, 2025 at 5:19 PM
requiring applicants to not only manually re-enter most of their transcript but also track down a course catalog to copy and paste course descriptions for dozens of courses from years to decades ago is absurd
wish i could just spend all day cloning
September 4, 2025 at 2:10 PM
wish i could just spend all day cloning
I will grudgingly admit that it is occasionally nice to spend a sunday morning reading Nevada and watching movies instead of being in the cell culture room
August 24, 2025 at 5:47 PM
I will grudgingly admit that it is occasionally nice to spend a sunday morning reading Nevada and watching movies instead of being in the cell culture room
I'm happy people can read the final version of this paper! See what happens when we detach heterochromatin from the nuclear periphery in mouse stem cells:
Another paper bluetorial! Today: how does the spatial location of genes influence their function? (1/n) www.nature.com/articles/s41...
The nuclear periphery confers repression on H3K9me2-marked genes and transposons to shape cell fate - Nature Cell Biology
Marin et al. report the role of lamin proteins and the lamin B receptor (LBR) in chromatin positioning at the nuclear periphery. Knockout of all lamins and LBR in mouse embryonic stem cells leads to h...
www.nature.com
July 23, 2025 at 1:21 AM
I'm happy people can read the final version of this paper! See what happens when we detach heterochromatin from the nuclear periphery in mouse stem cells:
Reposted by Charlie Allen
And yep - we found that the nuclear membrane protein LAP2b cooperates with lamin B1 to maintain nuclear integrity. When both are depleted, we see lamina defects and nuclear ruptures that look like this (cyan is nuclear lamina, magenta is nuclear integrity reporter): (5/n)
July 21, 2025 at 5:49 PM
And yep - we found that the nuclear membrane protein LAP2b cooperates with lamin B1 to maintain nuclear integrity. When both are depleted, we see lamina defects and nuclear ruptures that look like this (cyan is nuclear lamina, magenta is nuclear integrity reporter): (5/n)
Reposted by Charlie Allen
The nuclear lamina strengthens the nucleus and organizes the genome. So what happens when you acutely degrade it in living cells? Not what we thought! (1/n)
www.molbiolcell.org/doi/10.1091/...
www.molbiolcell.org/doi/10.1091/...
Lamin B1 and LAP2β resist cytoskeletal force to maintain lamin A/C meshwork organization and preserve nuclear integrity | Molecular Biology of the Cell
The nuclear lamins are extremely long-lived proteins in most cell types. As a consequence, lamin function cannot be effectively dissected with temporal precision using standard knock-down approaches. ...
www.molbiolcell.org
July 21, 2025 at 5:46 PM
The nuclear lamina strengthens the nucleus and organizes the genome. So what happens when you acutely degrade it in living cells? Not what we thought! (1/n)
www.molbiolcell.org/doi/10.1091/...
www.molbiolcell.org/doi/10.1091/...