Austin E. Y. T. Lefebvre
@austin-lefebvre.bsky.social
Dr. Leafbeaver, DS at Calico.
You can't spell imaging without "im aging" and boy am I.
Organelle enthusiast.
Creator of Nellie and Mitometer.
You can't spell imaging without "im aging" and boy am I.
Organelle enthusiast.
Creator of Nellie and Mitometer.
Pinned
Do you LOVE organelles??
Well I am THRILLED to announce that Nellie has been published in @naturemethods.bsky.social!
Nellie a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D, 3D, timelapse, multichannel live-cell microscopy
🧵1/N
Well I am THRILLED to announce that Nellie has been published in @naturemethods.bsky.social!
Nellie a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D, 3D, timelapse, multichannel live-cell microscopy
🧵1/N
I’m hiring for a microscopy image analysis role at Calico!
www.calicolabs.com/careers/?gh_...
We’re looking for someone at ~recent PhD lvl who has previous experience with biological samples and analyzing microscopy datasets.
Feel free to reach out with any questions!
www.calicolabs.com/careers/?gh_...
We’re looking for someone at ~recent PhD lvl who has previous experience with biological samples and analyzing microscopy datasets.
Feel free to reach out with any questions!
Careers - Calico
www.calicolabs.com
May 6, 2025 at 2:43 PM
I’m hiring for a microscopy image analysis role at Calico!
www.calicolabs.com/careers/?gh_...
We’re looking for someone at ~recent PhD lvl who has previous experience with biological samples and analyzing microscopy datasets.
Feel free to reach out with any questions!
www.calicolabs.com/careers/?gh_...
We’re looking for someone at ~recent PhD lvl who has previous experience with biological samples and analyzing microscopy datasets.
Feel free to reach out with any questions!
Reposted by Austin E. Y. T. Lefebvre
Analyzing organelles is HARD. They're complex, but critical to understanding biology. Current tools are manual, organelle-specific, or can't handle large 3D datasets.
With an easy install and a click of a button, Nellie solves ALL these problems!
nature.com/articles/s41...
🧵2/N
With an easy install and a click of a button, Nellie solves ALL these problems!
nature.com/articles/s41...
🧵2/N
Nellie: automated organelle segmentation, tracking and hierarchical feature extraction in 2D/3D live-cell microscopy - Nature Methods
Nellie is a comprehensive automated pipeline for studying the structure and intracellular dynamics of diverse organelles that offers accurate segmentation, tracking and feature extraction on both 2D a...
nature.com
February 27, 2025 at 8:13 PM
Analyzing organelles is HARD. They're complex, but critical to understanding biology. Current tools are manual, organelle-specific, or can't handle large 3D datasets.
With an easy install and a click of a button, Nellie solves ALL these problems!
nature.com/articles/s41...
🧵2/N
With an easy install and a click of a button, Nellie solves ALL these problems!
nature.com/articles/s41...
🧵2/N
Already some cool applications for Nellie: If you're used to MitoGraph data and want some of the same outputs from Nellie, check out Gav's Python code.
March 10, 2025 at 8:37 PM
Already some cool applications for Nellie: If you're used to MitoGraph data and want some of the same outputs from Nellie, check out Gav's Python code.
Reposted by Austin E. Y. T. Lefebvre
From Lefebvre and colleagues, Nellie is a comprehensive automated pipeline for studying the structure and intracellular dynamics of diverse organelles that offers accurate segmentation, tracking and feature extraction on both 2D and 3D data.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
March 6, 2025 at 4:52 PM
From Lefebvre and colleagues, Nellie is a comprehensive automated pipeline for studying the structure and intracellular dynamics of diverse organelles that offers accurate segmentation, tracking and feature extraction on both 2D and 3D data.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Austin E. Y. T. Lefebvre
We spend a lot of time thinking about segmenting cells, but organelles represent a similarly important challenge that is tricky due to their diversity in shapes and sizes. Please check out Nellie, a generalist tool for segmenting AND TRACKING organelles!
Do you LOVE organelles??
Well I am THRILLED to announce that Nellie has been published in @naturemethods.bsky.social!
Nellie a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D, 3D, timelapse, multichannel live-cell microscopy
🧵1/N
Well I am THRILLED to announce that Nellie has been published in @naturemethods.bsky.social!
Nellie a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D, 3D, timelapse, multichannel live-cell microscopy
🧵1/N
February 28, 2025 at 2:13 AM
We spend a lot of time thinking about segmenting cells, but organelles represent a similarly important challenge that is tricky due to their diversity in shapes and sizes. Please check out Nellie, a generalist tool for segmenting AND TRACKING organelles!
Do you LOVE organelles??
Well I am THRILLED to announce that Nellie has been published in @naturemethods.bsky.social!
Nellie a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D, 3D, timelapse, multichannel live-cell microscopy
🧵1/N
Well I am THRILLED to announce that Nellie has been published in @naturemethods.bsky.social!
Nellie a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D, 3D, timelapse, multichannel live-cell microscopy
🧵1/N
February 27, 2025 at 8:13 PM
Do you LOVE organelles??
Well I am THRILLED to announce that Nellie has been published in @naturemethods.bsky.social!
Nellie a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D, 3D, timelapse, multichannel live-cell microscopy
🧵1/N
Well I am THRILLED to announce that Nellie has been published in @naturemethods.bsky.social!
Nellie a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D, 3D, timelapse, multichannel live-cell microscopy
🧵1/N
Reposted by Austin E. Y. T. Lefebvre
Stoked to share TWO preprints on mitochondrial pearling! 🎉
We uncover how spontaneous #mitochondria pearling drives #mtDNA nucleoid distribution (doi.org/10.1101/2024...), and
@gavsturm.bsky.social
et al. dissect the biophysics behind it (doi.org/10.1101/2024...). Details below! 🔬🧵🧪
We uncover how spontaneous #mitochondria pearling drives #mtDNA nucleoid distribution (doi.org/10.1101/2024...), and
@gavsturm.bsky.social
et al. dissect the biophysics behind it (doi.org/10.1101/2024...). Details below! 🔬🧵🧪
December 23, 2024 at 4:37 PM
Stoked to share TWO preprints on mitochondrial pearling! 🎉
We uncover how spontaneous #mitochondria pearling drives #mtDNA nucleoid distribution (doi.org/10.1101/2024...), and
@gavsturm.bsky.social
et al. dissect the biophysics behind it (doi.org/10.1101/2024...). Details below! 🔬🧵🧪
We uncover how spontaneous #mitochondria pearling drives #mtDNA nucleoid distribution (doi.org/10.1101/2024...), and
@gavsturm.bsky.social
et al. dissect the biophysics behind it (doi.org/10.1101/2024...). Details below! 🔬🧵🧪