Han et al. use biochemistry and acute depletion of senataxin to reveal a role in RNAPII transcript elongation via reversal of pausing and backtracking. By contrast, senataxin has little direct effect on transcription termination.

Single-molecule fluorescence microscopy studies by Zhou et al. show that transcription activator proteins bind at UAS/enhancer DNA and then sequentially or simultaneously recruit Mediator coactivator and RNA polymerase II to form an initiation complex precursor. Multiple DNA-bound activators synergistically enhance recruitment by binding to the same Mediator complex.

Laboratory for Molecular and Systems Biology of RNA   The Legnini Group at Human Technopole combines molecular and systems biology approaches to study gene regulation. We use synthetic biology and opt...

Prokaryotes and eukaryotes use diverse strategies to cope with invading mobile genetic elements, including programmed DNA elimination (PDE). In the ciliate Paramecium , elimination of transposable ele...

Nature Reviews Molecular Cell Biology, Published online: 06 October 2025; doi:10.1038/s41580-025-00895-4Co-transcriptional mRNA maturation is a complex, multistep process. This Review focuses on how the development of long-read sequencing methods enabled investigating the timing, coordination and outcomes of alternative uses of transcription start sites, splice sites and polyadenylation sites and their disease implications.

This link will take you to a page that’s not on LinkedIn

Proteins operate through a few critical residues, yet most proteins remain uncharacterized at the deep molecular resolution, particularly within essential genes, where functional dissection is obstructed by lethality. Here, we establish a platform for mutational scanning of essential genes at their endogenous locus, combining a repressible complementation system with multiplexed CRISPR-based genome editing in budding yeast. Our approach provides a generalizable framework for dissecting essential protein function in vivo, expanding the capacity to map critical residues underlying essential cellular processes. We applied this strategy to NRD1, encoding an essential RNA Polymerase II (RNAPII) termination factor and performed a systematic alanine scanning with near-saturation coverage. We discovered novel and unexpected lethal mutations in the CTD-interacting domain (CID), thus revealing an unanticipated importance for this domain. Overall, our results demonstrate the power of our mutation scanning platform to map critical residues underlying essential cellular processes. ### Competing Interest Statement The authors have declared no competing interest. European Molecular Biology Organization, https://ror.org/04wfr2810, ALTF 889-2022

Vacancy at Department of Molecular Biology and Genetics - Protein Science, Aarhus University

Genome-wide and targeted perturbation of DNA methylation at centromeres affects CENP-A positioning and centromere structure, resulting in aneuploidy and reduced cell viability.

Promoters are DNA sequences that help to initiate transcription. Point mutations can create de-novo promoters, which can consequently transcribe inactive genes or create novel transcripts. We know lit...

Are you ready to lead groundbreaking research in Genome Biology? Join us at EMBL! We are seeking a motivated scientist to lead an independent research group addressing exciting and original biological...

Grossi et al. describe how sin-lncRNA interacts with the TCA enzyme DLST to maintain mitochondrial function and metabolic balance during senescence. Depletion of sin-lncRNA causes DLST translocation t...

The discovery that DNA methylation of different CpG sites can serve as digital barcodes of clonal identity led to the development of EPI-Clone, an algorithm that enables single-cell lineage tracing th...

Vacancy at Department of Molecular Biology and Genetics - BiRC - Bioinformatics Research Center, Aarhus University