Han Kim
@trancekr.bsky.social
The national center for inter-university research facilities at Seoul National University.
Cryo-EM / Structural Biology
Cryo-EM / Structural Biology
Attending the Scipion workshop at KAIST. Looking forward to learning a lot over the next 4 days
November 3, 2025 at 4:17 AM
Attending the Scipion workshop at KAIST. Looking forward to learning a lot over the next 4 days
Talented researchers from Rho lab at Seoul National University just published impressive results.
Glad I could help with some of the cryo-EM data collection for this work.
www.science.org/doi/10.1126/...
Glad I could help with some of the cryo-EM data collection for this work.
www.science.org/doi/10.1126/...
Structural dissection of αβ-tubulin heterodimer assembly and disassembly by human tubulin–specific chaperones
Microtubule assembly requires a set of chaperones known as tubulin-binding cofactors (TBCs). We used cryo–electron microscopy to visualize how human TBCD, TBCE, TBCC, and guanosine triphosphatase (GTP...
www.science.org
October 30, 2025 at 11:55 PM
Talented researchers from Rho lab at Seoul National University just published impressive results.
Glad I could help with some of the cryo-EM data collection for this work.
www.science.org/doi/10.1126/...
Glad I could help with some of the cryo-EM data collection for this work.
www.science.org/doi/10.1126/...
I think this will be my last post on this topic. I don’t want to keep talking about the authorship issue. Even though I wasn’t directly involved in the experiments/analyses for the paper, I believe this achievement wouldn’t have been possible without the discussions I led on SNS and the work
August 22, 2025 at 2:53 PM
I think this will be my last post on this topic. I don’t want to keep talking about the authorship issue. Even though I wasn’t directly involved in the experiments/analyses for the paper, I believe this achievement wouldn’t have been possible without the discussions I led on SNS and the work
Just received this response about why I was excluded from authorship on the small protein cryo-EM paper "We have to respect the opinions of the members who actually conducted the research. What they confirmed is that the research was conducted after Dr. Kim left the company." 1/3
August 22, 2025 at 5:28 AM
Just received this response about why I was excluded from authorship on the small protein cryo-EM paper "We have to respect the opinions of the members who actually conducted the research. What they confirmed is that the research was conducted after Dr. Kim left the company." 1/3
Reposted by Han Kim
PNCC Cryo-EM modeling and validation workshop on Sep 3-5, 2025. Register now! tinyurl.com/Cryo-EMModel...
August 20, 2025 at 11:36 PM
PNCC Cryo-EM modeling and validation workshop on Sep 3-5, 2025. Register now! tinyurl.com/Cryo-EMModel...
When I was at BaobabAiBIO, I was hesitant to publish the results because the PLK1 map was incomplete (I wrote about my concerns on Twitter).
Before quitting my job, I tried to finish at least these 2 things:
- getting a complete PLK1 map
- uploading MBP/PLK1 data to EMPIAR
Before quitting my job, I tried to finish at least these 2 things:
- getting a complete PLK1 map
- uploading MBP/PLK1 data to EMPIAR
This is an *excellent* review, and I hadn’t heard of PREreview before and I love the idea!
On another note (as you & Gabe highlight) looking at the PLK1 map really brings home that we need mandatory reporting of some kind of anisotropy metric - that map is not buildable, & def not close to 3Å IMHO
On another note (as you & Gabe highlight) looking at the PLK1 map really brings home that we need mandatory reporting of some kind of anisotropy metric - that map is not buildable, & def not close to 3Å IMHO
August 19, 2025 at 11:00 PM
When I was at BaobabAiBIO, I was hesitant to publish the results because the PLK1 map was incomplete (I wrote about my concerns on Twitter).
Before quitting my job, I tried to finish at least these 2 things:
- getting a complete PLK1 map
- uploading MBP/PLK1 data to EMPIAR
Before quitting my job, I tried to finish at least these 2 things:
- getting a complete PLK1 map
- uploading MBP/PLK1 data to EMPIAR
BTW, Jan-Hannes Schäfer @jhschaef.bsky.social and Gabriel C. Lander @landerlab.bsky.social provided an excellent PREreview of the paper. I really appreciate it.
prereview.org/reviews/1581...
prereview.org/reviews/1581...
August 19, 2025 at 12:29 PM
BTW, Jan-Hannes Schäfer @jhschaef.bsky.social and Gabriel C. Lander @landerlab.bsky.social provided an excellent PREreview of the paper. I really appreciate it.
prereview.org/reviews/1581...
prereview.org/reviews/1581...
Although I’m not on the author list, the MBP/PLK1 cryo-EM structure paper has been published on bioRxiv.
FYI, I was not involved in any writing or reviewing of the paper
www.biorxiv.org/content/10.1...
FYI, I was not involved in any writing or reviewing of the paper
www.biorxiv.org/content/10.1...
High-resolution cryo-EM structures of small protein–ligand complexes near the theoretical size limit
Cryo-electron microscopy (cryo-EM) is widely used to determine macromolecular structures at atomic resolution. The theoretical size lower limit of particles for cryo-EM analysis is 38 kDa, limited by ...
www.biorxiv.org
August 19, 2025 at 12:22 PM
Although I’m not on the author list, the MBP/PLK1 cryo-EM structure paper has been published on bioRxiv.
FYI, I was not involved in any writing or reviewing of the paper
www.biorxiv.org/content/10.1...
FYI, I was not involved in any writing or reviewing of the paper
www.biorxiv.org/content/10.1...
I’m experiencing repeated errors during tomography data acquisition.
Any suggestions on how to resolve this issue? Thanks in advance.
Any suggestions on how to resolve this issue? Thanks in advance.
May 4, 2025 at 11:55 PM
I’m experiencing repeated errors during tomography data acquisition.
Any suggestions on how to resolve this issue? Thanks in advance.
Any suggestions on how to resolve this issue? Thanks in advance.
Thrilled to see my former lab's paper on bioRxiv. Their graphene-based affinity grid successfully resolved low-abundance protein complexes. After such a long journey, it's wonderful to see this project finally producing good results!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
The graphene-based affinity cryo-EM grid for the endogenous protein structure determination
Following recent advancements in cryo-electron microscopy (cryo-EM) instrumentation and software algorithms, the next bottleneck in achieving high-resolution cryo-EM structures arises from sample prep...
www.biorxiv.org
February 28, 2025 at 12:34 AM
Thrilled to see my former lab's paper on bioRxiv. Their graphene-based affinity grid successfully resolved low-abundance protein complexes. After such a long journey, it's wonderful to see this project finally producing good results!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Han Kim
As we head into 2025, let's take one final look back at 2024 and how the year unfolded in the PDB archive. There were 15319 entries released last year but what more can we find out about those structures ⚖️?
A thread 1/11 🧵
A thread 1/11 🧵
January 13, 2025 at 9:43 AM
As we head into 2025, let's take one final look back at 2024 and how the year unfolded in the PDB archive. There were 15319 entries released last year but what more can we find out about those structures ⚖️?
A thread 1/11 🧵
A thread 1/11 🧵
Reposted by Han Kim
Come work with us! Only a few days remaining to apply for the #cryoET / #CLEM facility scientist position at the @icrlondon.bsky.social! #cryoEM
jobs.icr.ac.uk/vacancies/96...
jobs.icr.ac.uk/vacancies/96...
Higher Scientific Officer / Senior Scientific Officer – CLEM & cryoET scientist in Chelsea | The Institute of Cancer Research
View details and apply for this Higher Scientific Officer / Senior Scientific Officer – CLEM & cryoET scientist vacancy in Chelsea .
Salary : Commencement on the salary range is subject to comp...
jobs.icr.ac.uk
November 13, 2024 at 8:21 AM
Come work with us! Only a few days remaining to apply for the #cryoET / #CLEM facility scientist position at the @icrlondon.bsky.social! #cryoEM
jobs.icr.ac.uk/vacancies/96...
jobs.icr.ac.uk/vacancies/96...
New office desk & Krios G4 at SNU NCIRF
October 17, 2024 at 6:35 AM
New office desk & Krios G4 at SNU NCIRF
Starting from September, I will begin working at the Core Facility for Cell and Macromolecule Imaging and the National Center for Inter-university Research Facilities at Seoul National University to manage the cryo-EM equipment and provide research support.
September 3, 2024 at 11:49 PM
Starting from September, I will begin working at the Core Facility for Cell and Macromolecule Imaging and the National Center for Inter-university Research Facilities at Seoul National University to manage the cryo-EM equipment and provide research support.
Update on our kinase domain side project (37 kDa with a ligand): Previous sample had severe preferred orientation issue, pausing the project. Now back at it with new 2D classification results. Still preferred orientation persists, but we're optimistic about getting a map. keep the fingers crossed.
April 5, 2024 at 5:42 AM
Update on our kinase domain side project (37 kDa with a ligand): Previous sample had severe preferred orientation issue, pausing the project. Now back at it with new 2D classification results. Still preferred orientation persists, but we're optimistic about getting a map. keep the fingers crossed.
Our MBP cryo-EM structure is released.
Next uploading the micrographs to EMPIAR.
www.rcsb.org/structure/8YBE
Next uploading the micrographs to EMPIAR.
www.rcsb.org/structure/8YBE
March 6, 2024 at 11:42 AM
Our MBP cryo-EM structure is released.
Next uploading the micrographs to EMPIAR.
www.rcsb.org/structure/8YBE
Next uploading the micrographs to EMPIAR.
www.rcsb.org/structure/8YBE
Join us for the 2024 Cryo-EM Workshop for the pharma and biotech industries! Dive into the latest trends, techniques, and applications of Cryo-EM with experts from Baobab AiBIO and Thermo Fisher Scientific. (conducted in Korean / industry only) Mar 14, 10:00-17:00. docs.google.com/forms/d/e/1F...
February 17, 2024 at 3:01 AM
Join us for the 2024 Cryo-EM Workshop for the pharma and biotech industries! Dive into the latest trends, techniques, and applications of Cryo-EM with experts from Baobab AiBIO and Thermo Fisher Scientific. (conducted in Korean / industry only) Mar 14, 10:00-17:00. docs.google.com/forms/d/e/1F...
Oli B. Clarke (I’m mot sure he is using bluesky or not) asked about the ice thickness of our MBP dataset. So I checked the relative ice thickness plot in cryoSPARC and compared our membrane protein dataset. Here is the result.
November 21, 2023 at 1:55 AM
Oli B. Clarke (I’m mot sure he is using bluesky or not) asked about the ice thickness of our MBP dataset. So I checked the relative ice thickness plot in cryoSPARC and compared our membrane protein dataset. Here is the result.
the first time using a multi-hole grid!!!
October 26, 2023 at 5:03 AM
the first time using a multi-hole grid!!!
demoing Chameleon at Institute of Membrane Proteins/POSTECH
October 26, 2023 at 1:07 AM
demoing Chameleon at Institute of Membrane Proteins/POSTECH
Sharing our IMC20 poster here.
With all the feedbacks from twitter and IMC20, we will test more MBP samples internally and externally (with help from ThermoFisher). Stay tuned.
With all the feedbacks from twitter and IMC20, we will test more MBP samples internally and externally (with help from ThermoFisher). Stay tuned.
October 4, 2023 at 9:11 AM
Sharing our IMC20 poster here.
With all the feedbacks from twitter and IMC20, we will test more MBP samples internally and externally (with help from ThermoFisher). Stay tuned.
With all the feedbacks from twitter and IMC20, we will test more MBP samples internally and externally (with help from ThermoFisher). Stay tuned.
In Korea, currently observing the Chuseok (Korean Thanksgiving day) holiday.
October 2, 2023 at 9:16 AM
In Korea, currently observing the Chuseok (Korean Thanksgiving day) holiday.