Attending the Scipion workshop at KAIST. Looking forward to learning a lot over the next 4 days

After receiving this response, I'm so bewildered that I can't think straight. Looking at the paper's author contributions, 'H.K.' is included. Of course, there's no name corresponding to H.K. in the author list. 2/3

Thanks for the kind words, Oli.
The last time I processed the PLK1 map, it looked like the images I attached.
We also added 30° tilted micrographs for processing. It improved the orientation diagnostics results. However, the map is still anisotropic.

I’m experiencing repeated errors during tomography data acquisition.
Any suggestions on how to resolve this issue? Thanks in advance.

Snow in Seoul

New office desk & Krios G4 at SNU NCIRF

Update on our kinase domain side project (37 kDa with a ligand): Previous sample had severe preferred orientation issue, pausing the project. Now back at it with new 2D classification results. Still preferred orientation persists, but we're optimistic about getting a map. keep the fingers crossed.

Join us for the 2024 Cryo-EM Workshop for the pharma and biotech industries! Dive into the latest trends, techniques, and applications of Cryo-EM with experts from Baobab AiBIO and Thermo Fisher Scientific. (conducted in Korean / industry only) Mar 14, 10:00-17:00. docs.google.com/forms/d/e/1F...

Oli B. Clarke (I’m mot sure he is using bluesky or not) asked about the ice thickness of our MBP dataset. So I checked the relative ice thickness plot in cryoSPARC and compared our membrane protein dataset. Here is the result.

the first time using a multi-hole grid!!!

demoing Chameleon at Institute of Membrane Proteins/POSTECH

Sharing our IMC20 poster here.
With all the feedbacks from twitter and IMC20, we will test more MBP samples internally and externally (with help from ThermoFisher). Stay tuned.

In Korea, currently observing the Chuseok (Korean Thanksgiving day) holiday.