Ivan Radin
@radinbio.bsky.social
🌱🪴🌿🔬🏳️🌈
Lover of plants and microscopy.
Assistant Professor, Department of Plant and Microbial Biology, Univerisity of Minnesota.
Lab: radinlab.org/
Instagram: radinbio
Lover of plants and microscopy.
Assistant Professor, Department of Plant and Microbial Biology, Univerisity of Minnesota.
Lab: radinlab.org/
Instagram: radinbio
Pinned
Ivan Radin
@radinbio.bsky.social
· Jul 23
Best practices in plant fluorescence imaging and reporting: A primer
Abstract. Microscopy is a fundamental approach for plant cell and developmental biology as well as an essential tool for mechanistic studies in plant resea
academic.oup.com
Are you planning any plant fluorescent microscopy experiments? 🔬🌱🪴
Then this is a must-read. Here we provide tips and advice on how to improve your plant microscopy experiments and how to appropriately report your imaging results. ☺️
academic.oup.com/plcell/artic...
Then this is a must-read. Here we provide tips and advice on how to improve your plant microscopy experiments and how to appropriately report your imaging results. ☺️
academic.oup.com/plcell/artic...
During my postdoc, I looked at hundreds of images like this 🤩
These are two apical caulonemal cells from moss Physcomitrium patens stained with MDY64 (shown in shades of orange). The natural autofluorescence of chlorophyll is in cyan.
#microscopymonday #moss #plantcells #plantmicroscopy
These are two apical caulonemal cells from moss Physcomitrium patens stained with MDY64 (shown in shades of orange). The natural autofluorescence of chlorophyll is in cyan.
#microscopymonday #moss #plantcells #plantmicroscopy
November 10, 2025 at 3:33 PM
During my postdoc, I looked at hundreds of images like this 🤩
These are two apical caulonemal cells from moss Physcomitrium patens stained with MDY64 (shown in shades of orange). The natural autofluorescence of chlorophyll is in cyan.
#microscopymonday #moss #plantcells #plantmicroscopy
These are two apical caulonemal cells from moss Physcomitrium patens stained with MDY64 (shown in shades of orange). The natural autofluorescence of chlorophyll is in cyan.
#microscopymonday #moss #plantcells #plantmicroscopy
Check out this new paper on the Arabidopsis MLS8 channel and its importance for oscillatory growth
and cell wall dynamics in pollen tubes!
link.springer.com/article/10.1...
and cell wall dynamics in pollen tubes!
link.springer.com/article/10.1...
Mechanosensitive ion channel MSL8 is required for oscillatory growth and cell wall dynamics in Arabidopsis pollen tubes - Plant Reproduction
The male gametophyte in flowering plants, pollen, both performs the critical role of fertilization and represents a unique and accessible system for interrogating plant cell mechanics. A key component...
link.springer.com
November 7, 2025 at 4:20 PM
Check out this new paper on the Arabidopsis MLS8 channel and its importance for oscillatory growth
and cell wall dynamics in pollen tubes!
link.springer.com/article/10.1...
and cell wall dynamics in pollen tubes!
link.springer.com/article/10.1...
In preparation for Halloween, these chloroplasts had a seance! 👻🎃
These five Arabidopsis chloroplasts were extending their stromules (protrusions of the chloroplast envelope and stroma) towards each other.
#microscopymonday #Arabidopsis #plantcells #plantmicroscopy
These five Arabidopsis chloroplasts were extending their stromules (protrusions of the chloroplast envelope and stroma) towards each other.
#microscopymonday #Arabidopsis #plantcells #plantmicroscopy
October 27, 2025 at 3:44 PM
In preparation for Halloween, these chloroplasts had a seance! 👻🎃
These five Arabidopsis chloroplasts were extending their stromules (protrusions of the chloroplast envelope and stroma) towards each other.
#microscopymonday #Arabidopsis #plantcells #plantmicroscopy
These five Arabidopsis chloroplasts were extending their stromules (protrusions of the chloroplast envelope and stroma) towards each other.
#microscopymonday #Arabidopsis #plantcells #plantmicroscopy
Reposted by Ivan Radin
Genetically engineered color-changing Arabidopsis 🧬📷- attempt #3
I think I finally nailed it with this one.
I think I finally nailed it with this one.
October 27, 2025 at 11:53 AM
Genetically engineered color-changing Arabidopsis 🧬📷- attempt #3
I think I finally nailed it with this one.
I think I finally nailed it with this one.
This image of moss Physcomitrium patens beautifully demonstrates what we mean when we say that this cell type is characterized by oblique cell walls.
You can see that the cell walls between cells are at an angle and not perpendicular to the cell axis.
#microscopymonday #moss #plantcells
You can see that the cell walls between cells are at an angle and not perpendicular to the cell axis.
#microscopymonday #moss #plantcells
October 20, 2025 at 1:51 PM
This image of moss Physcomitrium patens beautifully demonstrates what we mean when we say that this cell type is characterized by oblique cell walls.
You can see that the cell walls between cells are at an angle and not perpendicular to the cell axis.
#microscopymonday #moss #plantcells
You can see that the cell walls between cells are at an angle and not perpendicular to the cell axis.
#microscopymonday #moss #plantcells
Here are some “leaf” or phyllid cells from moss Physcomitrium patens gametophore. Cells are expressing a green fluorescence marker that labels the vacuolar membrane. Chloroplasts' autofluorescence is in magenta.
Can you spot dividing chloroplasts?
#microscopymonday #moss #plantcells
Can you spot dividing chloroplasts?
#microscopymonday #moss #plantcells
October 13, 2025 at 2:40 PM
Here are some “leaf” or phyllid cells from moss Physcomitrium patens gametophore. Cells are expressing a green fluorescence marker that labels the vacuolar membrane. Chloroplasts' autofluorescence is in magenta.
Can you spot dividing chloroplasts?
#microscopymonday #moss #plantcells
Can you spot dividing chloroplasts?
#microscopymonday #moss #plantcells
Reposted by Ivan Radin
I started to work on MscS-Like proteins 20 years ago, dreaming that they were involved in plant mechanotransduction. And it is becoming more & more clear that they are!
#plantscience 🧪
"DmMSL10 is crucial for mechanosensing, facilitating AP firing by generating a receptor potential (RP) amplitude."
#plantscience 🧪
"DmMSL10 is crucial for mechanosensing, facilitating AP firing by generating a receptor potential (RP) amplitude."
🍀🔬
MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap @natcomms.nature.com from Toyota lab.
www.nature.com/articles/s41...
MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap @natcomms.nature.com from Toyota lab.
www.nature.com/articles/s41...
October 7, 2025 at 4:48 PM
I started to work on MscS-Like proteins 20 years ago, dreaming that they were involved in plant mechanotransduction. And it is becoming more & more clear that they are!
#plantscience 🧪
"DmMSL10 is crucial for mechanosensing, facilitating AP firing by generating a receptor potential (RP) amplitude."
#plantscience 🧪
"DmMSL10 is crucial for mechanosensing, facilitating AP firing by generating a receptor potential (RP) amplitude."
Reposted by Ivan Radin
Inside a thylakoid membrane
The molecular architecture of the thylakoid membrane in a vascular plant has been determined with single-molecule precision.
buff.ly/U9TzrOh
The molecular architecture of the thylakoid membrane in a vascular plant has been determined with single-molecule precision.
buff.ly/U9TzrOh
October 8, 2025 at 10:02 AM
Inside a thylakoid membrane
The molecular architecture of the thylakoid membrane in a vascular plant has been determined with single-molecule precision.
buff.ly/U9TzrOh
The molecular architecture of the thylakoid membrane in a vascular plant has been determined with single-molecule precision.
buff.ly/U9TzrOh
This is a section of the cotyledon (first leaf) surface from the flowering plant Arabidopsis thaliana, which is expressing the green fluorescent protein vacuolar membrane marker (vac-GB). Chloroplasts are in magenta, visible due to the autofluorescence of the chlorophyll.
#microscopymonday
#microscopymonday
October 6, 2025 at 3:55 PM
This is a section of the cotyledon (first leaf) surface from the flowering plant Arabidopsis thaliana, which is expressing the green fluorescent protein vacuolar membrane marker (vac-GB). Chloroplasts are in magenta, visible due to the autofluorescence of the chlorophyll.
#microscopymonday
#microscopymonday
In case you missed it, our workshop on Fiji Basics for Visualization and Quantification of Plant Images is now available on YouTube:
www.youtube.com/watch?v=4TMJ...
We give a lot of practical tips on how to get started with Fiji.
Keep an eye out for announcements on follow-up workshops.
www.youtube.com/watch?v=4TMJ...
We give a lot of practical tips on how to get started with Fiji.
Keep an eye out for announcements on follow-up workshops.
Imaging Workshop Webinar Series: FIJI Basics for Visualizing and Quantifying Plant Images
YouTube video by Plant Cell Atlas
www.youtube.com
October 1, 2025 at 3:17 PM
In case you missed it, our workshop on Fiji Basics for Visualization and Quantification of Plant Images is now available on YouTube:
www.youtube.com/watch?v=4TMJ...
We give a lot of practical tips on how to get started with Fiji.
Keep an eye out for announcements on follow-up workshops.
www.youtube.com/watch?v=4TMJ...
We give a lot of practical tips on how to get started with Fiji.
Keep an eye out for announcements on follow-up workshops.
Reposted by Ivan Radin
In Two Days!
Attendees: we encourage you to download and install FIJI in advance of the workshop if you wish to follow along on your own computer. Keep an eye on your email & visit:
hpc.nih.gov/apps/Fiji and fiji.sc
Register Now! bit.ly/45zJE7V
Attendees: we encourage you to download and install FIJI in advance of the workshop if you wish to follow along on your own computer. Keep an eye on your email & visit:
hpc.nih.gov/apps/Fiji and fiji.sc
Register Now! bit.ly/45zJE7V
September 22, 2025 at 3:28 PM
In Two Days!
Attendees: we encourage you to download and install FIJI in advance of the workshop if you wish to follow along on your own computer. Keep an eye on your email & visit:
hpc.nih.gov/apps/Fiji and fiji.sc
Register Now! bit.ly/45zJE7V
Attendees: we encourage you to download and install FIJI in advance of the workshop if you wish to follow along on your own computer. Keep an eye on your email & visit:
hpc.nih.gov/apps/Fiji and fiji.sc
Register Now! bit.ly/45zJE7V
This is what happens when you remove the wall from plant cells. They become perfectly spherical and are called protoplasts.
These protoplasts were isolated from Arabidopsis and transformed with a vector to express cytosolic GFP (in orange). The chloroplast autofluorescence is in grey.
#microscopy
These protoplasts were isolated from Arabidopsis and transformed with a vector to express cytosolic GFP (in orange). The chloroplast autofluorescence is in grey.
#microscopy
September 22, 2025 at 7:36 PM
This is what happens when you remove the wall from plant cells. They become perfectly spherical and are called protoplasts.
These protoplasts were isolated from Arabidopsis and transformed with a vector to express cytosolic GFP (in orange). The chloroplast autofluorescence is in grey.
#microscopy
These protoplasts were isolated from Arabidopsis and transformed with a vector to express cytosolic GFP (in orange). The chloroplast autofluorescence is in grey.
#microscopy
Our Plant and Microbial Biology Graduate Program (cbs.umn.edu/pmb/graduate...) is organizing two Zoom Admissions open houses where you can learn about preparing a strong application and get advice about graduate school from current PMB students.
Drop in at any point on either or both days, see below
Drop in at any point on either or both days, see below
Plant and Microbial Biology Graduate Program | College of Biological Sciences
cbs.umn.edu
September 20, 2025 at 9:30 PM
Our Plant and Microbial Biology Graduate Program (cbs.umn.edu/pmb/graduate...) is organizing two Zoom Admissions open houses where you can learn about preparing a strong application and get advice about graduate school from current PMB students.
Drop in at any point on either or both days, see below
Drop in at any point on either or both days, see below
Do you know what happens when you touch a carnivorous sundew plant?
If the touch is strong and large enough, a cytosolic calcium wave will spread from the site of touch throughout the whole plant, but if you only touch one tentacle (see post below), the calcium wave will be local and less intense.
If the touch is strong and large enough, a cytosolic calcium wave will spread from the site of touch throughout the whole plant, but if you only touch one tentacle (see post below), the calcium wave will be local and less intense.
September 15, 2025 at 2:26 PM
Do you know what happens when you touch a carnivorous sundew plant?
If the touch is strong and large enough, a cytosolic calcium wave will spread from the site of touch throughout the whole plant, but if you only touch one tentacle (see post below), the calcium wave will be local and less intense.
If the touch is strong and large enough, a cytosolic calcium wave will spread from the site of touch throughout the whole plant, but if you only touch one tentacle (see post below), the calcium wave will be local and less intense.
This might be one of the simplest images I captured, but very fascinating nonetheless, as it shows how tight the cytoplasm in a plant cell is. The cytosol and all other organelles are squished between the plasma membrane (magenta) and the central vacuole (its membrane is green)
#MicroscopyMonday
#MicroscopyMonday
September 8, 2025 at 4:22 PM
This might be one of the simplest images I captured, but very fascinating nonetheless, as it shows how tight the cytoplasm in a plant cell is. The cytosol and all other organelles are squished between the plasma membrane (magenta) and the central vacuole (its membrane is green)
#MicroscopyMonday
#MicroscopyMonday
We love working with our moss, Physcomitrium (formerly Physcomitrella) patens.
In this dissecting microscope image, you can see the juvenile protonema filaments spread away from the main plant as they explore the new areas.
#MicroscopyMonday #Moss #Plants
In this dissecting microscope image, you can see the juvenile protonema filaments spread away from the main plant as they explore the new areas.
#MicroscopyMonday #Moss #Plants
September 1, 2025 at 5:16 PM
We love working with our moss, Physcomitrium (formerly Physcomitrella) patens.
In this dissecting microscope image, you can see the juvenile protonema filaments spread away from the main plant as they explore the new areas.
#MicroscopyMonday #Moss #Plants
In this dissecting microscope image, you can see the juvenile protonema filaments spread away from the main plant as they explore the new areas.
#MicroscopyMonday #Moss #Plants
Did you know that plants can have different types of plastids/chloroplasts? The small green plastids are from the surface (epidermal) cells of the Arabidopsis leaf, while the much bigger magenta plastids are from the deeper spongy tissue. See video below.
#MicroscopyMonday #cellbiology #plastids
#MicroscopyMonday #cellbiology #plastids
August 25, 2025 at 2:20 PM
Did you know that plants can have different types of plastids/chloroplasts? The small green plastids are from the surface (epidermal) cells of the Arabidopsis leaf, while the much bigger magenta plastids are from the deeper spongy tissue. See video below.
#MicroscopyMonday #cellbiology #plastids
#MicroscopyMonday #cellbiology #plastids
Reposted by Ivan Radin
About 250 scientists from all over the world will meet at the University of Münster from 24 to 29 August for the "Chlamy 2025" conference to discuss the latest research findings on microalgae.
A focus on the microalgae ‘Chlamy’
About 250 scientists from all over the world will meet at the University of Münster from 24 to 29 August for the "Chlamy 2025" conference to discuss the latest research findings on microalgae.
www.uni-muenster.de
August 22, 2025 at 1:36 PM
About 250 scientists from all over the world will meet at the University of Münster from 24 to 29 August for the "Chlamy 2025" conference to discuss the latest research findings on microalgae.
Reposted by Ivan Radin
Members of PCA Publish A Guide to Elevate Plant Fluorescence Microscopy.
Read More Here:
www.danforthcenter.org/news/global-...
Read More Here:
www.danforthcenter.org/news/global-...
Global Team of Experts Publish Guide to Elevate Plant Fluorescence Microscopy - Danforth Plant Science Center
A team of expert scientists led by Kirk Czymmek, PhD, director of the Advanced Bioimaging Laboratory at the Donald Danforth Plant Science Center and Heather E. McFarlane, assistant professor at the Un...
www.danforthcenter.org
August 21, 2025 at 4:15 PM
Members of PCA Publish A Guide to Elevate Plant Fluorescence Microscopy.
Read More Here:
www.danforthcenter.org/news/global-...
Read More Here:
www.danforthcenter.org/news/global-...
Reposted by Ivan Radin
☘️🔬
Amyloplasts are necessary for full gravitropism in thallus of Marchantia polymorpha @jxbotany.bsky.social
doi.org/10.1093/jxb/...
Amyloplasts are necessary for full gravitropism in thallus of Marchantia polymorpha @jxbotany.bsky.social
doi.org/10.1093/jxb/...
August 21, 2025 at 3:27 PM
☘️🔬
Amyloplasts are necessary for full gravitropism in thallus of Marchantia polymorpha @jxbotany.bsky.social
doi.org/10.1093/jxb/...
Amyloplasts are necessary for full gravitropism in thallus of Marchantia polymorpha @jxbotany.bsky.social
doi.org/10.1093/jxb/...
A fun experiment! I injected the bacteria Agrobacterium tumefaciens into the tobacco leaf, Nicotiana benthamiana. The bacteria transferred some DNA into plant cells, forcing them to make GFP (green fluorescent protein). I used blue light to excite GFP.
Guess which leaf was infiltrated! #fluorescence
Guess which leaf was infiltrated! #fluorescence
August 18, 2025 at 1:50 PM
A fun experiment! I injected the bacteria Agrobacterium tumefaciens into the tobacco leaf, Nicotiana benthamiana. The bacteria transferred some DNA into plant cells, forcing them to make GFP (green fluorescent protein). I used blue light to excite GFP.
Guess which leaf was infiltrated! #fluorescence
Guess which leaf was infiltrated! #fluorescence
Reposted by Ivan Radin
We are excited to announce the PCA Imaging Workshop Webinar Series: FIJI Basics for Visualizing and Quantifying Plant Images!
This workshop will be held on Wednesday, September 24, 2025 at 7:30am PT/9:30am CT/10:30am ET.
Register Now! bit.ly/45zJE7V
@radinbio.bsky.social @rheelab.bsky.social
This workshop will be held on Wednesday, September 24, 2025 at 7:30am PT/9:30am CT/10:30am ET.
Register Now! bit.ly/45zJE7V
@radinbio.bsky.social @rheelab.bsky.social
August 15, 2025 at 5:05 PM
We are excited to announce the PCA Imaging Workshop Webinar Series: FIJI Basics for Visualizing and Quantifying Plant Images!
This workshop will be held on Wednesday, September 24, 2025 at 7:30am PT/9:30am CT/10:30am ET.
Register Now! bit.ly/45zJE7V
@radinbio.bsky.social @rheelab.bsky.social
This workshop will be held on Wednesday, September 24, 2025 at 7:30am PT/9:30am CT/10:30am ET.
Register Now! bit.ly/45zJE7V
@radinbio.bsky.social @rheelab.bsky.social
Reposted by Ivan Radin
This weekend, the PCA CNP held our annual meeting in Portland, Maine to discuss the state of the PCA RCN: how far we've come and what the future looks like. While there, we networked, shared ideas, and discussed our goals for the next phase of the PCA RCN.
August 10, 2025 at 2:32 PM
This weekend, the PCA CNP held our annual meeting in Portland, Maine to discuss the state of the PCA RCN: how far we've come and what the future looks like. While there, we networked, shared ideas, and discussed our goals for the next phase of the PCA RCN.
Not all microscopy images turn out great. Nevertheless, they can still be cool. This one definitely has an abstract art vibe. 🖼️
#MicroscopyMonday #Fluoresence #CellBiology
#MicroscopyMonday #Fluoresence #CellBiology
August 11, 2025 at 2:03 PM
Not all microscopy images turn out great. Nevertheless, they can still be cool. This one definitely has an abstract art vibe. 🖼️
#MicroscopyMonday #Fluoresence #CellBiology
#MicroscopyMonday #Fluoresence #CellBiology
This is what you see when you zoom in on fungus, growing uninvitedly on our "sterile" moss plates. What a beautiful hyphal network. #Fungi #Beatifulcontamination #Imaging #Microscopymonday
August 4, 2025 at 1:38 PM
This is what you see when you zoom in on fungus, growing uninvitedly on our "sterile" moss plates. What a beautiful hyphal network. #Fungi #Beatifulcontamination #Imaging #Microscopymonday