NanoTag Biotechnologies
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nanotag.bsky.social
NanoTag Biotechnologies
@nanotag.bsky.social
𝐂𝐮𝐬𝐭𝐨𝐦 𝐒𝐢𝐧𝐠𝐥𝐞-𝐃𝐨𝐦𝐚𝐢𝐧 𝐀𝐧𝐭𝐢𝐛𝐨𝐝𝐲 𝐃𝐢𝐬𝐜𝐨𝐯𝐞𝐫𝐲 & 𝐍𝐚𝐧𝐨𝐛𝐨𝐝𝐲-𝐁𝐚𝐬𝐞𝐝 𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐓𝐨𝐨𝐥𝐬
Did you know #multiplex IF can be done in just one incubation step?

❌ No sequential staining
❌ No host-species limits
❌ No secondary-induced clustering

🔬Pre-mix your favourite primary antibodies (even same-species) with Smart Secondaries® #nanobody & apply directly to your sample for OneStep IF.🤩
February 19, 2026 at 10:24 AM
Neuroscientists - looking for a high-affinity gift?💝

Inside the postsynapse, proteins crowd the show
PSD95’s the star - but hard to know.

IgG antibodies knock, but can’t get through
Too bulky to reach the target they pursue.

But FluoTag®-X2 anti-PSD95 slips right in
Binds PSD95. Precise within.
February 13, 2026 at 11:32 AM
Heading to #BPS2026 in San Francisco?

Visit NanoTag team at booth 720 to explore our single-domain antibody #sdAb tools: ALFA® System, FluoTags®, Smart Secondaries® & Selector Resins.
See you! 👋

@biophysicalsoc.bsky.social
#Nanobody #Multiplexing
#SuperResolution #Microscopy
#AffinityPurification
February 12, 2026 at 9:39 AM
📄This new Journal of Microscopy 🔬 paper uses rigorous single-molecule assays to benchmark secondary nanobodies and shows NanoTag #SmartSecondaries® deliver high-affinity binding, efficient saturation, and exceptionally low off-target labeling for robust #multiplexed immunoassays 💪
🔬 In this #OpenAccess paper, the authors propose a set of experiments spanning total internal reflection fluorescence and confocal microscopies that can be used to validate secondary nanobody binding, specificity, and their propensity for mis-targeted binding in multiplex assays.
February 6, 2026 at 5:30 PM
💬 Thank you, Bhanu Prakash Potlapalli, for the great feedback on the ALFA® system! 😊 Applied in a CRISPR–dCas9 DNA imaging approach for efficient labeling of repetitive DNA sequences and live-cell imaging of plant telomeres doi.org/10.1093/jxb/...

Explore #ALFAsystem 👉 nano-tag.com/technology/t...
February 3, 2026 at 10:28 AM
Did you know? #NanoTag offers custom single-domain antibody (sdAb) discovery services, including alpaca immunization🦙and synthetic library–based approaches🧬backed by over a decade of experience in the field 💪.

Discuss your project with our experts: nano-tag.com/contact/

#CRO #Nanobody #VHH #sdAb
January 28, 2026 at 1:08 PM
🔬 Join Imaging Bites seminar 28 Jan at 12:30 at @kingscollegelondon.bsky.social.
Prof. Dr. Felipe Opazo @opazo.bsky.social presents #OneStep-IF, a simplified approach to highly #multiplexed IF using same-species primaries and Smart Secondaries® premixes.

Register here: www.kcl.ac.uk/events/imagi...
January 21, 2026 at 11:25 AM
Reposted by NanoTag Biotechnologies
✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
December 22, 2025 at 10:45 AM
🎄 A glimpse into #NanoTag Christmas dinner - Glühwein outdoors, a shared dinner, and Schrottwichteln 🎁♻️ where funny, used items are exchanged and find a new home. The past year has been special for us as we celebrate 10 years. Grateful for our team, customers, and partners.
Here’s to what’s next 🥂
December 22, 2025 at 10:36 AM
#CellBio2025 in Philadelphia has come to a close!
Thanks to everyone who stopped by the #NanoTag booth 🦙 – great conversations and insights all around.
We were excited to hear how our sdAb tools are driving your new discoveries 📄👏

#SingleDomainAntibody
#ALFAsystem
#SmartSecondaries
December 11, 2025 at 2:07 PM
What most people don’t see is the behind-the-scenes effort- not magic, just #teamwork. Karina & Steffen have #NanoTag booth ready for #CellBio2025 in Philadelphia. Starting Sunday, discover how our single-domain antibody solutions can support your research🔬🦙.
See you ascbiology.bsky.social 👋
December 6, 2025 at 12:51 PM
Attending #CellBio2025 in Philadelphia Dec 6–10?
Visit #NanoTag team at booth #1117 to explore our #sdAb technologies & ready-to-use reagents: ALFA® System, FluoTags®, Smart Secondaries®, Selector Resins.
See you there! 👋 @ascbiology.bsky.social

#Nanobody #CellBiology
#Immunofluorescence #PullDown
November 25, 2025 at 9:38 AM
Join our CTO, Felipe Opazo @opazo.bsky.social, at #MBSJ2025 @mbsj-official.bsky.social as he presents new super-resolution #multiplexing advances powered by #SmartSecondaries®.
🔬Discover flexible, scalable and precise multi-target imaging.

#Nanobody
#NanoPlex #SUMPAINT
#SuperResolution #Microscopy
November 21, 2025 at 11:07 AM
🌱🔬 #FluorescenceFriday Beautiful plant microscopy revealed with the ALFA-tag & NbALFA. A recent study by @plantubiquitin.bsky.social et al in Plant Commun shows how well #ALFA®system works in planta. From imaging #SuperResolution to protein interactions, degradation and more.
doi.org/10.1016/j.xp...
November 14, 2025 at 9:42 AM
Have you heard of #ANGEL?
Not this 😇, but ALFA Nanobody-Guided Endogenous Labeling #ANGEL - the new approach published by Wang et al. in @natchembio.nature.com Build on our ALFA® System, it enables direct fluorescent readout of #CRISPR knock-ins.

📄Full paper here: bit.ly/47XgK48
ALFA nanobody-guided endogenous labeling - Nature Chemical Biology
Small peptide tags (like ALFA) cause minimal disruption of proteins, but they typically lack fluorescence, which would enable knockin screening. Now, antigen-stabilizing fluorescent protein-fused nano...
bit.ly
November 6, 2025 at 1:28 PM
This #Halloween 🦙🧛‍♂️ Alpacula brewed up #SMLM treat!
No more horror stories of no blinking & black holes ⚫️🔬Our single-domain anti-BOOdies 👻 #FluoTags and #SmartSecondaries now glow with self-blinking JF635b✨, delivering frightfully precise and simple #dSTORM imaging. 👉 bit.ly/4ffIkdB
October 30, 2025 at 1:06 PM
#NanoTag is exhibiting at #BIOEurope 2025 🇦🇹 Nov 3–5! Meet our team in Vienna at the German Pavilion, booth C120-18, to explore how our 🦙 sdAb discovery platforms and protein engineering services advance life science and biomedical research.

#SingleDomainAntibodies
#ProteinProduction
#CROservices
October 28, 2025 at 9:18 AM
#NanoTag joins the Life Science Ecosystem at Nordic Life Science Days #NLSDays2025 in Gothenburg!🇸🇪 Meet our CEO Hansjörg Götzke at the Booth B03:46 (German Pavilion), and discover our sdAb platforms🦙, protein production services, and research tools🔬advancing #LifeScience & #Biomedicine.
October 7, 2025 at 8:17 AM
Reposted by NanoTag Biotechnologies
And #GEF25 "best goodies for people who have kids" award goes to... @nanotag.bsky.social!
And yes, I used my badge holder to store the gummy alpacas 😅
September 30, 2025 at 6:19 PM
🔬✨ #GEF25 Expansion Microscopy Forum in Göttingen just wrapped up! We were happy to support as sponsors and share our sdAb innovations for super-resolution #microscopy. Thanks to the organizers & all participants for an inspiring event! 👏

#ExM #SmartSecondaries #Multiplexing
September 30, 2025 at 1:12 PM
🦙 NanoTag at #SingleDomainAntibodies Conference 2025!

Our CEO Hansjörg Götzke will be in Utrecht sharing how our immune & synthetic sdAb platforms drive advances in research, diagnostics & therapeutics.

Our founder Felipe Opazo @opazo.bsky.social will also be there to connect & share insights.
September 25, 2025 at 9:22 AM
🆕 Looking for a reliable reagent for efficient human B-cell stimulation?

Meet NanoTag’s Human B-cell Activator (hBCA): tetramerized sdAb for fast, consistent, Fc-free B-cell activation.

👉 Learn more here: bit.ly/3KldYMF

#Immunology #BCRclustering #Nanobody
September 23, 2025 at 12:47 PM
#ExM turned 10 this year - just like #NanoTag! 🎉
Join us & the super-resolution community in Göttingen for the ExM Forum! 🔬Hear from our founder Felipe Opazo @opazo.bsky.social and CSO Steffen Frey on how NanoTag’s sdAb solutions empower nanoscopy & #multiplex imaging.

See you in Göttingen!
#GEF25
September 17, 2025 at 8:33 AM
10 years of #NanoTag Biotechnologies! 🥳

We celebrated the milestone with a sunny raft trip on the Oker river. ☀️
Big thanks to our amazing team, partners & customers for being part of the journey.💙

Here’s to the next chapter! 🥂

#10Years #Teamwork #Biotech
September 15, 2025 at 8:09 AM
Reposted by NanoTag Biotechnologies
The 𝗔𝗟𝗙𝗔-𝘁𝗮𝗴 𝗶𝘀 𝗮 𝘀𝗺𝗮𝗹𝗹 𝗿𝗮𝘁𝗶𝗼𝗻𝗮𝗹𝗹𝘆 𝗱𝗲𝘀𝗶𝗴𝗻𝗲𝗱 𝗽𝗿𝗼𝘁𝗲𝗶𝗻 𝘁𝗮𝗴 that facilitates highly specific labeling when combined with the anti-ALFA single-domain antibody (rdcu.be/eFy86 ).
The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
Nature Communications - Epitope tags are widely used in various applications, but often lack versatility. Here, the authors introduce a small, alpha helical tag, which is recognized by a high...
rdcu.be
September 11, 2025 at 8:52 AM