Megan Harwig 🔬
@mcharwig.bsky.social
Mom, mitochondriac and confocal microscopist. Microscopy Core Manager at the Medical College of Wisconsin.
Opinions = mine
https://orcid.org/0000-0003-2140-5739
https://mcw.ilab.agilent.com/service_center/show_external/5443/electron_microscopy_facility
Opinions = mine
https://orcid.org/0000-0003-2140-5739
https://mcw.ilab.agilent.com/service_center/show_external/5443/electron_microscopy_facility
Reposted by Megan Harwig 🔬
We are hiring an Imaging Scientis. Apply here: go.mbl.edu/AS1887
October 28, 2025 at 6:43 PM
We are hiring an Imaging Scientis. Apply here: go.mbl.edu/AS1887
We are looking for perfusion/microfluidics systems that are compatible with OKO chambers on our Dragonfly spinning disk confocal microscopes in our core. Does anyone have any recommendations of products you love that do not leak? 🔬🧪🧬🥼
October 29, 2025 at 3:50 AM
We are looking for perfusion/microfluidics systems that are compatible with OKO chambers on our Dragonfly spinning disk confocal microscopes in our core. Does anyone have any recommendations of products you love that do not leak? 🔬🧪🧬🥼
Soon our core will be getting a MicroPoint4 tool for our Dragonfly 620SR. Anyone else have a MicroPoint tool? What has been your favorite application?
andor.oxinst.com/learning/vie...
andor.oxinst.com/learning/vie...
Glia Responses after Injury with Laser Ablation in Zebrafish- Oxford Instruments
Discover how the Kucenas Lab uses our confocal microscopes and precise laser ablation to study glial development, injury response, and dynamics in Zebrafish.
andor.oxinst.com
October 17, 2025 at 2:36 AM
Soon our core will be getting a MicroPoint4 tool for our Dragonfly 620SR. Anyone else have a MicroPoint tool? What has been your favorite application?
andor.oxinst.com/learning/vie...
andor.oxinst.com/learning/vie...
Come work with us at MCW! This position is in the Translational Metabolomics-Preclinical Imaging Shared Resource. This position involves imaging mice, rats and other small animal models with multimodal imaging.
careers.peopleclick.com/careerscp/cl...
careers.peopleclick.com/careerscp/cl...
Research Scientist I
Medical College of Wisconsin - Research Scientist I - Milwaukee WI 53201
careers.peopleclick.com
October 10, 2025 at 1:00 AM
Come work with us at MCW! This position is in the Translational Metabolomics-Preclinical Imaging Shared Resource. This position involves imaging mice, rats and other small animal models with multimodal imaging.
careers.peopleclick.com/careerscp/cl...
careers.peopleclick.com/careerscp/cl...
Reposted by Megan Harwig 🔬
It has been a dream come true to lead a rotation in the @mblscience.bsky.social Physiology course - thanks to the amazing Directors @gladfelterlab.bsky.social and @brangwynnelab.bsky.social, course staff, TAs, and students!
July 12, 2025 at 11:43 PM
It has been a dream come true to lead a rotation in the @mblscience.bsky.social Physiology course - thanks to the amazing Directors @gladfelterlab.bsky.social and @brangwynnelab.bsky.social, course staff, TAs, and students!
Reposted by Megan Harwig 🔬
If you work in #VolumeEM, please fill in our survey to help us build a Collaborative Computational Project for volumeEM!
Survey link: forms.office.com/e/eMAJGCKUnc
we're aiming to identify the needs of the community in order to build a roadmap for all-things data-related in #vEM
Survey link: forms.office.com/e/eMAJGCKUnc
we're aiming to identify the needs of the community in order to build a roadmap for all-things data-related in #vEM
May 29, 2025 at 7:14 PM
If you work in #VolumeEM, please fill in our survey to help us build a Collaborative Computational Project for volumeEM!
Survey link: forms.office.com/e/eMAJGCKUnc
we're aiming to identify the needs of the community in order to build a roadmap for all-things data-related in #vEM
Survey link: forms.office.com/e/eMAJGCKUnc
we're aiming to identify the needs of the community in order to build a roadmap for all-things data-related in #vEM
Reposted by Megan Harwig 🔬
The recording of our recent webinar, "Next-generation expansion microscopy: 20× magnification in one step" by @shiweiw.bsky.social, is now available! You can access it at: bio-protocol.org/webinar/deta...
#Webinar #LifeSciences
#Webinar #LifeSciences
May 31, 2025 at 10:04 AM
The recording of our recent webinar, "Next-generation expansion microscopy: 20× magnification in one step" by @shiweiw.bsky.social, is now available! You can access it at: bio-protocol.org/webinar/deta...
#Webinar #LifeSciences
#Webinar #LifeSciences
Reposted by Megan Harwig 🔬
Our groundbreaking Dark Sectioning technique is now published in Nature Methods! 🎉 It supercharges existing imaging systems:
🔬 Widefield → Confocal
🔬 Single-photon → Multiphoton
🔬 2D-SIM → 3D-SIM
Now integrated in Airy PolarSIM and #FlexSIM!
doi.org/10.1038/s415...
#Microscopy #Fluorescence
🔬 Widefield → Confocal
🔬 Single-photon → Multiphoton
🔬 2D-SIM → 3D-SIM
Now integrated in Airy PolarSIM and #FlexSIM!
doi.org/10.1038/s415...
#Microscopy #Fluorescence
May 16, 2025 at 3:03 AM
Our groundbreaking Dark Sectioning technique is now published in Nature Methods! 🎉 It supercharges existing imaging systems:
🔬 Widefield → Confocal
🔬 Single-photon → Multiphoton
🔬 2D-SIM → 3D-SIM
Now integrated in Airy PolarSIM and #FlexSIM!
doi.org/10.1038/s415...
#Microscopy #Fluorescence
🔬 Widefield → Confocal
🔬 Single-photon → Multiphoton
🔬 2D-SIM → 3D-SIM
Now integrated in Airy PolarSIM and #FlexSIM!
doi.org/10.1038/s415...
#Microscopy #Fluorescence
Reposted by Megan Harwig 🔬
Revolutionizing High-Speed Dynamic Fluorescence Imaging with Deep Learning-Enhanced Denoising Techniques
Researchers have unveiled a groundbreaking advancement in the field of fluorescence microscopy, designed to resolve one of the most significant challenges facing scientists: image degradation…
Researchers have unveiled a groundbreaking advancement in the field of fluorescence microscopy, designed to resolve one of the most significant challenges facing scientists: image degradation…
Revolutionizing High-Speed Dynamic Fluorescence Imaging with Deep Learning-Enhanced Denoising Techniques
Researchers have unveiled a groundbreaking advancement in the field of fluorescence microscopy, designed to resolve one of the most significant challenges facing scientists: image degradation due to noise in dynamic in vivo imaging. This innovative method, recently published in the journal PhotoniX, presents a self-supervised deep learning approach known as Temporal-gradient empowered Denoising (TeD). The newly developed technique stands to revolutionize the way researchers capture and analyze high-speed biological processes, which are often severely obscured by noise.
scienmag.com
May 29, 2025 at 4:53 PM
Revolutionizing High-Speed Dynamic Fluorescence Imaging with Deep Learning-Enhanced Denoising Techniques
Researchers have unveiled a groundbreaking advancement in the field of fluorescence microscopy, designed to resolve one of the most significant challenges facing scientists: image degradation…
Researchers have unveiled a groundbreaking advancement in the field of fluorescence microscopy, designed to resolve one of the most significant challenges facing scientists: image degradation…
Reposted by Megan Harwig 🔬
⭐ The 2025 BioTechniques Image Competition is now open! 🔬
You have until 17 June to submit up to ten scientific images or illustrations that you’ve taken or created during your research.
Prizes, rules and how to enter >>> www.biotechniques.com/life-science... 🧪
#imaging #microscopy
You have until 17 June to submit up to ten scientific images or illustrations that you’ve taken or created during your research.
Prizes, rules and how to enter >>> www.biotechniques.com/life-science... 🧪
#imaging #microscopy
BioTechniques Image Competition 2025
Submit your scientific images to the BioTechniques Scientific Image Competition 2025! Enter now for the chance to win a US$200 gift card.
www.biotechniques.com
May 27, 2025 at 2:45 PM
⭐ The 2025 BioTechniques Image Competition is now open! 🔬
You have until 17 June to submit up to ten scientific images or illustrations that you’ve taken or created during your research.
Prizes, rules and how to enter >>> www.biotechniques.com/life-science... 🧪
#imaging #microscopy
You have until 17 June to submit up to ten scientific images or illustrations that you’ve taken or created during your research.
Prizes, rules and how to enter >>> www.biotechniques.com/life-science... 🧪
#imaging #microscopy
Reposted by Megan Harwig 🔬
🤖 How resilient are deep learning models to real-world imperfections in microscopy?
🔗 Practical guidelines for cell segmentation models under optical aberrations in microscopy, DOI: doi.org/10.1016/j.cs...
📚 CSBJ Quantum Biology and Biophotonics: www.csbj.org/qbio
#CellSegmentation #Microscopy
🔗 Practical guidelines for cell segmentation models under optical aberrations in microscopy, DOI: doi.org/10.1016/j.cs...
📚 CSBJ Quantum Biology and Biophotonics: www.csbj.org/qbio
#CellSegmentation #Microscopy
May 27, 2025 at 10:21 PM
🤖 How resilient are deep learning models to real-world imperfections in microscopy?
🔗 Practical guidelines for cell segmentation models under optical aberrations in microscopy, DOI: doi.org/10.1016/j.cs...
📚 CSBJ Quantum Biology and Biophotonics: www.csbj.org/qbio
#CellSegmentation #Microscopy
🔗 Practical guidelines for cell segmentation models under optical aberrations in microscopy, DOI: doi.org/10.1016/j.cs...
📚 CSBJ Quantum Biology and Biophotonics: www.csbj.org/qbio
#CellSegmentation #Microscopy
Reposted by Megan Harwig 🔬
Today is the deadline to apply! Come hang out with our team in Boston and learn to think about bioimage analysis and how to use many fun open source tools!
BIG NEWS - for the first time ever, we're running a Bioimage Analysis Beginnings Bootcamp. Learn #bioimageanalysis from awesome folks, and gain familiarity with some new tools as well as how to pick the right tool in the first place. Aug 4th-8th - apply here (soon!) forms.gle/d2fCXsrWHVP8...
May 21, 2025 at 2:35 PM
Today is the deadline to apply! Come hang out with our team in Boston and learn to think about bioimage analysis and how to use many fun open source tools!
Reposted by Megan Harwig 🔬
Did you know that Bioimage Archive has a specific database for people to share AI-ready imaging datasets? I did not until @yanlanmao.bsky.social told me! Please deposit! If people regularly submitted, it would have a huge collective impact on bioimage analysis. www.ebi.ac.uk/bioimage-arc...
BioImageArchive < The European Bioinformatics Institute < EMBL-EBI
The BioImageArchive – a free, publicly available online resource which stores and distributes biological images
www.ebi.ac.uk
May 21, 2025 at 2:53 PM
Did you know that Bioimage Archive has a specific database for people to share AI-ready imaging datasets? I did not until @yanlanmao.bsky.social told me! Please deposit! If people regularly submitted, it would have a huge collective impact on bioimage analysis. www.ebi.ac.uk/bioimage-arc...
Reposted by Megan Harwig 🔬
Efficient, standards-based #bioimaging data management is essential for research and reuse. Join the presentation about “A Journey to #FAIR Bioimage Data,” a contribution to
@ebi.embl.org's STANDFLOW project webinar series.
🗓️ July 2, 2025 | 02:30–03:30 pm
👉 Register: www.ebi.ac.uk/training/eve...
@ebi.embl.org's STANDFLOW project webinar series.
🗓️ July 2, 2025 | 02:30–03:30 pm
👉 Register: www.ebi.ac.uk/training/eve...
May 20, 2025 at 1:29 PM
Efficient, standards-based #bioimaging data management is essential for research and reuse. Join the presentation about “A Journey to #FAIR Bioimage Data,” a contribution to
@ebi.embl.org's STANDFLOW project webinar series.
🗓️ July 2, 2025 | 02:30–03:30 pm
👉 Register: www.ebi.ac.uk/training/eve...
@ebi.embl.org's STANDFLOW project webinar series.
🗓️ July 2, 2025 | 02:30–03:30 pm
👉 Register: www.ebi.ac.uk/training/eve...
Reposted by Megan Harwig 🔬
📄 A recent review article covers the latest in next-generation #Fluorophores and site-specific labeling strategies for different types of biomolecules, including info on their application in areas such as #SuperResolution #Microscopy: bit.ly/3Fhbeh9
#Fluorescence
#Fluorescence
May 19, 2025 at 12:35 PM
📄 A recent review article covers the latest in next-generation #Fluorophores and site-specific labeling strategies for different types of biomolecules, including info on their application in areas such as #SuperResolution #Microscopy: bit.ly/3Fhbeh9
#Fluorescence
#Fluorescence
Reposted by Megan Harwig 🔬
A suite of bridged rhodamine dyes for fluorescence microscopy, named BriDyes, offers excellent brightness, photostability, solubility, cell permeability, and resistance to photoblueing.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
May 19, 2025 at 7:36 PM
A suite of bridged rhodamine dyes for fluorescence microscopy, named BriDyes, offers excellent brightness, photostability, solubility, cell permeability, and resistance to photoblueing.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Megan Harwig 🔬
Carlos Oscar S. Sorzano et al.: Image-processing methods for electron microscopy of biological specimens ... ##IUCrhttps://journals.iucr.org/paper?S2059798325004449
Image-processing methods for electron microscopy of biological specimens
The focused issue on Image-processing methods for electron microscopy of biological specimens is introduced. The virtual issue is available at https://journals.iucr.org/special_issues/2025/imageprocessing.
journals.iucr.org
May 20, 2025 at 12:00 AM
Carlos Oscar S. Sorzano et al.: Image-processing methods for electron microscopy of biological specimens ... ##IUCrhttps://journals.iucr.org/paper?S2059798325004449
Reposted by Megan Harwig 🔬
By democratizing bioimage analysis, BiaPy is paving the way for faster, more inclusive scientific breakthroughs, bringing us closer to understanding the building blocks of life.
https://mappingignorance.org/2025/05/15/biapy-analising-bioimages-made-easy/
https://mappingignorance.org/2025/05/15/biapy-analising-bioimages-made-easy/
May 15, 2025 at 7:05 PM
By democratizing bioimage analysis, BiaPy is paving the way for faster, more inclusive scientific breakthroughs, bringing us closer to understanding the building blocks of life.
https://mappingignorance.org/2025/05/15/biapy-analising-bioimages-made-easy/
https://mappingignorance.org/2025/05/15/biapy-analising-bioimages-made-easy/
Reposted by Megan Harwig 🔬
Ever wonder which microscopes power today’s life science breakthroughs? 🧬🔬 I put together a quick guide—from brightfield to holography.
Check it out 👉 www.linkedin.com/pulse/seeing...
#Microscopy #LifeSciences #Biotech #SpatialBiology #ImagingScience #SciComm
Check it out 👉 www.linkedin.com/pulse/seeing...
#Microscopy #LifeSciences #Biotech #SpatialBiology #ImagingScience #SciComm
Seeing Is Believing: The Microscopes Powering Modern Life Sciences
As someone who works closely with life science researchers and imaging technologies, I often get a surprisingly common question: "You sell microscopes? Like for high school labs? Or on Amazon? Or for ...
www.linkedin.com
May 15, 2025 at 9:52 PM
Ever wonder which microscopes power today’s life science breakthroughs? 🧬🔬 I put together a quick guide—from brightfield to holography.
Check it out 👉 www.linkedin.com/pulse/seeing...
#Microscopy #LifeSciences #Biotech #SpatialBiology #ImagingScience #SciComm
Check it out 👉 www.linkedin.com/pulse/seeing...
#Microscopy #LifeSciences #Biotech #SpatialBiology #ImagingScience #SciComm
Reposted by Megan Harwig 🔬
Good morning! Today we publish three new #MicroscoPills about #confocal #microscopy.
E03 - What is it and why is it useful in #biology
E04 - Confocal microscopy history and evolution
E05 - The role of the confocal #pinhole
Follow us on Spotify
open.spotify.com/show/20hJTM4...
#science
E03 - What is it and why is it useful in #biology
E04 - Confocal microscopy history and evolution
E05 - The role of the confocal #pinhole
Follow us on Spotify
open.spotify.com/show/20hJTM4...
#science
MicroscoPills: your bite-sized dose of microscopy concepts
Podcast · Dr Alessandro Esposito · Unlock the tiny world with short episodes on microscopy and its application to biology and biomedical research. Discover how these tools visualise life at the molecu...
open.spotify.com
May 11, 2025 at 7:04 AM
Good morning! Today we publish three new #MicroscoPills about #confocal #microscopy.
E03 - What is it and why is it useful in #biology
E04 - Confocal microscopy history and evolution
E05 - The role of the confocal #pinhole
Follow us on Spotify
open.spotify.com/show/20hJTM4...
#science
E03 - What is it and why is it useful in #biology
E04 - Confocal microscopy history and evolution
E05 - The role of the confocal #pinhole
Follow us on Spotify
open.spotify.com/show/20hJTM4...
#science
Reposted by Megan Harwig 🔬
Thanks to expansion microscopy, clever labeling, and modern segmentation approaches, doing connectomics with #light #microscopy has become feasible - huge congratulations Mojtaba & the Danzl lab at @istaresearch.bsky.social !
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Light-microscopy-based connectomic reconstruction of mammalian brain tissue - Nature
A technique called LICONN (light-microscopy-based connectomics) allows mapping of brain tissue at synapse level and simultaneous measurement of molecular information, thus enabling quantification of c...
www.nature.com
May 8, 2025 at 2:52 AM
Thanks to expansion microscopy, clever labeling, and modern segmentation approaches, doing connectomics with #light #microscopy has become feasible - huge congratulations Mojtaba & the Danzl lab at @istaresearch.bsky.social !
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Megan Harwig 🔬
FREE Help with Bioimage Analysis!
Second round of hashtag#GloBIAS Free Help (previously Call4Help) is happening in May. You can register for the sessions on our webpage: www.globias.org/activities/g...
Registration deadline is 7. May 2025.
Registration deadline is 7. May 2025.
May 5, 2025 at 7:42 PM
FREE Help with Bioimage Analysis!
Reposted by Megan Harwig 🔬
Deadline extended to 19 May! 🧪 Learn about light sheet #microscopy, sample preparation, data processing and #ImageAnalysis. Apply now for EMBO Practical Course on Light sheet microscopy in Dresden, Germany, 11–22 August
meetings.embo.org/event/25-lsm
#EMBOlsm #imaging #EMBOevents
meetings.embo.org/event/25-lsm
#EMBOlsm #imaging #EMBOevents
Light sheet microscopy
Light sheet microscopy is a technology that enables near-isotropic imaging of large biological specimens over time. It opens new avenues to study biological processes with unprecedented imaging speed…
meetings.embo.org
May 6, 2025 at 5:52 AM
Deadline extended to 19 May! 🧪 Learn about light sheet #microscopy, sample preparation, data processing and #ImageAnalysis. Apply now for EMBO Practical Course on Light sheet microscopy in Dresden, Germany, 11–22 August
meetings.embo.org/event/25-lsm
#EMBOlsm #imaging #EMBOevents
meetings.embo.org/event/25-lsm
#EMBOlsm #imaging #EMBOevents
Reposted by Megan Harwig 🔬
May 5, 2025 at 5:54 PM
Reposted by Megan Harwig 🔬
BIG NEWS - for the first time ever, we're running a Bioimage Analysis Beginnings Bootcamp. Learn #bioimageanalysis from awesome folks, and gain familiarity with some new tools as well as how to pick the right tool in the first place. Aug 4th-8th - apply here (soon!) forms.gle/d2fCXsrWHVP8...
May 1, 2025 at 9:22 PM
BIG NEWS - for the first time ever, we're running a Bioimage Analysis Beginnings Bootcamp. Learn #bioimageanalysis from awesome folks, and gain familiarity with some new tools as well as how to pick the right tool in the first place. Aug 4th-8th - apply here (soon!) forms.gle/d2fCXsrWHVP8...