Mathieu Lupien
@matlupien.bsky.social
Senior scientist @Princess Margaret Cancer Centre, Prof @UofT. BHAG: Conquer cancer by treating it as a disease of the chromatin. https://lupienlab.uhnresearch.ca/
Pinned
Mathieu Lupien
@matlupien.bsky.social
· Dec 23
Prostate cancer cells converge to an inflammatory-like state upon metastatic dissemination - Nature Communications
Understanding tumor heterogeneity and its impact on prostate cancer progression remains elusive. Here, single nucleus snATAC and snRNA sequencing of a multi-loci sampled cohort of advanced prostate ca...
www.nature.com
Hot from the press: Using single-cell chromatin accessibility and gene expression across ~300,000 cells, we show that prostate cancer dissemination involves phenotypic plasticity over clonal selection, with malignant cells converging toward an inflammatory-like state during lymph node spread.
New study from Dr. Hansen Housheng He’s team, thrilled that we contributed, on functional mapping of m6A in cancer at scale with implications for novel target discovery and future therapeutic development. www.nature.com/articles/s43...
METTL3-based epitranscriptomic editing screening identifies functional m6A sites in cancers - Nature Cancer
He and colleagues develop a METTL3-based RNA base-editing screening strategy to identify functional N6-methyladenosine (m6A) sites that are important for prostate cancer cells and validate the role of...
www.nature.com
February 6, 2026 at 4:32 PM
New study from Dr. Hansen Housheng He’s team, thrilled that we contributed, on functional mapping of m6A in cancer at scale with implications for novel target discovery and future therapeutic development. www.nature.com/articles/s43...
Reposted by Mathieu Lupien
Our new study found that, when prostate cancer cells spread, they adopt an “inflammatory-like” state and mimic immune cells, suggesting new ways to control cancer progression.
Co-led by @matlupien.bsky.social @uhn.ca @uhnresearch.ca with @dkfz.bsky.social:
doi.org/10.1038/s414...
Co-led by @matlupien.bsky.social @uhn.ca @uhnresearch.ca with @dkfz.bsky.social:
doi.org/10.1038/s414...
January 2, 2026 at 3:43 PM
Our new study found that, when prostate cancer cells spread, they adopt an “inflammatory-like” state and mimic immune cells, suggesting new ways to control cancer progression.
Co-led by @matlupien.bsky.social @uhn.ca @uhnresearch.ca with @dkfz.bsky.social:
doi.org/10.1038/s414...
Co-led by @matlupien.bsky.social @uhn.ca @uhnresearch.ca with @dkfz.bsky.social:
doi.org/10.1038/s414...
Reposted by Mathieu Lupien
Back-to-back: Integrating single-nuclei RNA-seq and whole-genome sequencing, @joachimwei.bsky.social and his team show how prostate cancer metastasis is shaped by plasticity-driven convergence alongside ongoing clonal evolution, with distinct clones adopting shared transcriptional trajectories.
Clonal evolution and transcriptional plasticity shape metastatic dissemination routes in prostate cancer - Nature Communications
The impact of tumour heterogeneity on metastatic potential in prostate cancer remains poorly understood. Here, the analysis of single nuclei RNA sequencing and whole-genome sequencing from samples fro...
www.nature.com
December 23, 2025 at 9:15 PM
Back-to-back: Integrating single-nuclei RNA-seq and whole-genome sequencing, @joachimwei.bsky.social and his team show how prostate cancer metastasis is shaped by plasticity-driven convergence alongside ongoing clonal evolution, with distinct clones adopting shared transcriptional trajectories.
Reposted by Mathieu Lupien
Hot from the press: Using single-cell chromatin accessibility and gene expression across ~300,000 cells, we show that prostate cancer dissemination involves phenotypic plasticity over clonal selection, with malignant cells converging toward an inflammatory-like state during lymph node spread.
Prostate cancer cells converge to an inflammatory-like state upon metastatic dissemination - Nature Communications
Understanding tumor heterogeneity and its impact on prostate cancer progression remains elusive. Here, single nucleus snATAC and snRNA sequencing of a multi-loci sampled cohort of advanced prostate ca...
www.nature.com
December 23, 2025 at 9:15 PM
Hot from the press: Using single-cell chromatin accessibility and gene expression across ~300,000 cells, we show that prostate cancer dissemination involves phenotypic plasticity over clonal selection, with malignant cells converging toward an inflammatory-like state during lymph node spread.
Hot from the press: Using single-cell chromatin accessibility and gene expression across ~300,000 cells, we show that prostate cancer dissemination involves phenotypic plasticity over clonal selection, with malignant cells converging toward an inflammatory-like state during lymph node spread.
Prostate cancer cells converge to an inflammatory-like state upon metastatic dissemination - Nature Communications
Understanding tumor heterogeneity and its impact on prostate cancer progression remains elusive. Here, single nucleus snATAC and snRNA sequencing of a multi-loci sampled cohort of advanced prostate ca...
www.nature.com
December 23, 2025 at 9:15 PM
Hot from the press: Using single-cell chromatin accessibility and gene expression across ~300,000 cells, we show that prostate cancer dissemination involves phenotypic plasticity over clonal selection, with malignant cells converging toward an inflammatory-like state during lymph node spread.
Reposted by Mathieu Lupien
A major output of the 4D Nucleome project appeared today. This is the joint effort of many scientists working together and (publicly) sharing data and results for several years. We hope this is of interest to many genome biologists!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
An integrated view of the structure and function of the human 4D nucleome - Nature
The 4D Nucleome Project demonstrates the use of genomic assays and computational methods to measure genome folding and then predict genomic structure from DNA sequence, facilitatin...
www.nature.com
December 17, 2025 at 8:59 PM
A major output of the 4D Nucleome project appeared today. This is the joint effort of many scientists working together and (publicly) sharing data and results for several years. We hope this is of interest to many genome biologists!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Mathieu Lupien
Exciting new conference!
🔔 Don’t forget: Abstract submissions for #CBHC2026 are open!
🗓️ Deadline: Feb 12, 2026
📥 Submit here: https://f.mtr.cool/oftnlpqjlg
🗓️ Deadline: Feb 12, 2026
📥 Submit here: https://f.mtr.cool/oftnlpqjlg
December 12, 2025 at 8:59 PM
Exciting new conference!
Reposted by Mathieu Lupien
Our lab website just got a major refresh to better present:
Our research philosophy: why chromatin is central to our work.
A snapshot of our culture: diverse, collaborative, impact-driven.
Our priorities: how we translate discoveries into clinical reality.
Check it out
Our research philosophy: why chromatin is central to our work.
A snapshot of our culture: diverse, collaborative, impact-driven.
Our priorities: how we translate discoveries into clinical reality.
Check it out
Lupien Lab
lupienlab.uhnresearch.ca
November 27, 2025 at 10:42 PM
Our lab website just got a major refresh to better present:
Our research philosophy: why chromatin is central to our work.
A snapshot of our culture: diverse, collaborative, impact-driven.
Our priorities: how we translate discoveries into clinical reality.
Check it out
Our research philosophy: why chromatin is central to our work.
A snapshot of our culture: diverse, collaborative, impact-driven.
Our priorities: how we translate discoveries into clinical reality.
Check it out
Reposted by Mathieu Lupien
Confused by all the histones that are cropping up in organisms that are decidedly NOT eukaryotes? check out our review - fantastic work by team NucEvo in the #Lugerlab
The Expanding Histone Universe: Histone-Based DNA Organization in Noneukaryotic Organisms - www.annualreviews.org/content/jour...
The Expanding Histone Universe: Histone-Based DNA Organization in Noneukaryotic Organisms - www.annualreviews.org/content/jour...
December 9, 2025 at 3:14 PM
Confused by all the histones that are cropping up in organisms that are decidedly NOT eukaryotes? check out our review - fantastic work by team NucEvo in the #Lugerlab
The Expanding Histone Universe: Histone-Based DNA Organization in Noneukaryotic Organisms - www.annualreviews.org/content/jour...
The Expanding Histone Universe: Histone-Based DNA Organization in Noneukaryotic Organisms - www.annualreviews.org/content/jour...
Our lab website just got a major refresh to better present:
Our research philosophy: why chromatin is central to our work.
A snapshot of our culture: diverse, collaborative, impact-driven.
Our priorities: how we translate discoveries into clinical reality.
Check it out
Our research philosophy: why chromatin is central to our work.
A snapshot of our culture: diverse, collaborative, impact-driven.
Our priorities: how we translate discoveries into clinical reality.
Check it out
Lupien Lab
lupienlab.uhnresearch.ca
November 27, 2025 at 10:42 PM
Our lab website just got a major refresh to better present:
Our research philosophy: why chromatin is central to our work.
A snapshot of our culture: diverse, collaborative, impact-driven.
Our priorities: how we translate discoveries into clinical reality.
Check it out
Our research philosophy: why chromatin is central to our work.
A snapshot of our culture: diverse, collaborative, impact-driven.
Our priorities: how we translate discoveries into clinical reality.
Check it out
Reposted by Mathieu Lupien
One of our top-rated posts on @altmetric.com this past was published in @nataging.nature.com. You can read 'Multilingualism protects against accelerated aging in cross-sectional and longitudinal analyses of 27 European countries' here: spklr.io/63325BMpTZ
Multilingualism protects against accelerated aging in cross-sectional and longitudinal analyses of 27 European countries - Nature Aging
In cross-sectional and longitudinal analyses of 86,149 participants across 27 European countries, Amoruso, Hernandez and colleagues identify multilingualism as a protective factor against accelerated aging, using biobehavioral age gaps.
spklr.io
November 14, 2025 at 8:07 PM
One of our top-rated posts on @altmetric.com this past was published in @nataging.nature.com. You can read 'Multilingualism protects against accelerated aging in cross-sectional and longitudinal analyses of 27 European countries' here: spklr.io/63325BMpTZ
Lest We Forget
N'oublions Jamais
N'oublions Jamais
November 11, 2025 at 3:59 PM
Lest We Forget
N'oublions Jamais
N'oublions Jamais
DNA replication is only half the story. Cells actually replicate their chromatin, ensuring that both the DNA sequences and the chromatin states are faithfully maintained to preserve cell identity and function after division. Here's a glimpse of how this process unfolds, courtesy of theCrux:
Epigenetic Replication during DNA Replication - Replicating histones and DNA methylation
YouTube video by theCrux
www.youtube.com
November 11, 2025 at 3:08 PM
DNA replication is only half the story. Cells actually replicate their chromatin, ensuring that both the DNA sequences and the chromatin states are faithfully maintained to preserve cell identity and function after division. Here's a glimpse of how this process unfolds, courtesy of theCrux:
Reposted by Mathieu Lupien
Fantastic opportunity at The Hospital for Sick Children. Please repost. www.nature.com/naturecareer...
SCIENTIST – Developmental, Stem Cell & Cancer Biology Program - Toronto (City), Ontario (CA) job with The Hospital for Sick Children - Developmental & Stem Cell Biology Program | 12848200
Discovery-based and/or translational research using model organism genetics and/or stem cell and organoid platforms to study paediatric cancer.
www.nature.com
October 28, 2025 at 10:06 PM
Fantastic opportunity at The Hospital for Sick Children. Please repost. www.nature.com/naturecareer...
Reposted by Mathieu Lupien
We have two fantastically well-funded #PhD studentships available through the @evomg-dn.bsky.social #EU doctoral network (led by brilliant @mirimiam.bsky.social, @crg.eu). These enable students to move to the UK and come and work with us @icr.ac.uk to exploit #cancer #evolution for patient benefit.
October 14, 2025 at 8:21 PM
We have two fantastically well-funded #PhD studentships available through the @evomg-dn.bsky.social #EU doctoral network (led by brilliant @mirimiam.bsky.social, @crg.eu). These enable students to move to the UK and come and work with us @icr.ac.uk to exploit #cancer #evolution for patient benefit.
Reposted by Mathieu Lupien
Wonderful opportunity to work with Martin Howard and my group trying to understand how chromatin influences gene transcription and expression. Integration of theory, modelling, and experimentation. Come and join the team!
Please repost!
jobs.jic.ac.uk/Details.asp?...
Please repost!
jobs.jic.ac.uk/Details.asp?...
John Innes Centre
jobs.jic.ac.uk
October 8, 2025 at 9:04 AM
Wonderful opportunity to work with Martin Howard and my group trying to understand how chromatin influences gene transcription and expression. Integration of theory, modelling, and experimentation. Come and join the team!
Please repost!
jobs.jic.ac.uk/Details.asp?...
Please repost!
jobs.jic.ac.uk/Details.asp?...
Reposted by Mathieu Lupien
❓Can a father’s environmental exposures before conception influence their offspring?
We systematically tackled this - identifying effects of paternal age, environment and genetics on early embryos - as well as confounding influences
www.embopress.org/doi/full/10....
We systematically tackled this - identifying effects of paternal age, environment and genetics on early embryos - as well as confounding influences
www.embopress.org/doi/full/10....
Embryonic signatures of intergenerational epigenetic inheritance across paternal environments and genetic backgrounds | The EMBO Journal
imageimagePaternal environmental exposures have been linked with modulation of phenotype and
disease risk in offspring via largely unclear mechanisms. This study employs in vitro
fertilization and sin...
www.embopress.org
September 27, 2025 at 7:28 AM
❓Can a father’s environmental exposures before conception influence their offspring?
We systematically tackled this - identifying effects of paternal age, environment and genetics on early embryos - as well as confounding influences
www.embopress.org/doi/full/10....
We systematically tackled this - identifying effects of paternal age, environment and genetics on early embryos - as well as confounding influences
www.embopress.org/doi/full/10....
Reposted by Mathieu Lupien
Exciting FULL PROFESSORSHIP at the Vienna BioCenter - Molecular Mechanisms of Disease Mechanisms: www.maxperutzlabs.ac.at/news/open-po... Come be our colleague!
Full Professorship in Molecular Mechanisms of Disease
The Max Perutz Labs - one of Europe’s leading institutes for basic research in the life sciences - invite applications for a Full Professorship in Molecular Mechanisms of Disease. We are seeking outst...
www.maxperutzlabs.ac.at
September 4, 2025 at 10:25 AM
Exciting FULL PROFESSORSHIP at the Vienna BioCenter - Molecular Mechanisms of Disease Mechanisms: www.maxperutzlabs.ac.at/news/open-po... Come be our colleague!
Every so often, a paper lands at exactly the right moment. This one’s a gem. Can’t wait to try out this new strategy for delivering CRISPRoff chromatin-editing tools into our favorite cellular model system. Congrats to James K Nuñez and his team: www.nature.com/articles/s41...
Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins - Nature Communications
Epigenome editing programs gene silencing without inducing DNA breaks but challenges in delivery into human cells limit its broader use. Here, the authors present the RENDER platform, which uses virus...
www.nature.com
August 29, 2025 at 7:27 PM
Every so often, a paper lands at exactly the right moment. This one’s a gem. Can’t wait to try out this new strategy for delivering CRISPRoff chromatin-editing tools into our favorite cellular model system. Congrats to James K Nuñez and his team: www.nature.com/articles/s41...
Reposted by Mathieu Lupien
Excited to see this published with additional data following our preprint a while back. Cool combination (in our biased view) of controlled TF expression and machine learning to decode chromatin sensitivity. www.sciencedirect.com/science/arti....
August 7, 2025 at 4:20 PM
Excited to see this published with additional data following our preprint a while back. Cool combination (in our biased view) of controlled TF expression and machine learning to decode chromatin sensitivity. www.sciencedirect.com/science/arti....
Reposted by Mathieu Lupien
This acquisition will bring together two pioneering companies with a shared vision: to make single cell analysis more powerful, affordable and accessible to researchers worldwide.
Learn more: bit.ly/4mAWAS8
Learn more: bit.ly/4mAWAS8
August 7, 2025 at 8:14 PM
This acquisition will bring together two pioneering companies with a shared vision: to make single cell analysis more powerful, affordable and accessible to researchers worldwide.
Learn more: bit.ly/4mAWAS8
Learn more: bit.ly/4mAWAS8
One often hears that DNA is the blueprint of life. Today, I read a really nice paper that begins with "proteins are the cornerstone of life". By that logic, I might start referring to RNA as the scaffold, and chromatin as the framework that shapes it all.
Regardless, the paper is a must-read:
Regardless, the paper is a must-read:
Comprehensive human proteome profiles across a 50-year lifespan reveal aging trajectories and signatures
Proteins are the cornerstone of life. However, the proteomic blueprint of aging across human tissues remains uncharted. Here, we present a comprehensi…
www.sciencedirect.com
July 31, 2025 at 7:19 PM
One often hears that DNA is the blueprint of life. Today, I read a really nice paper that begins with "proteins are the cornerstone of life". By that logic, I might start referring to RNA as the scaffold, and chromatin as the framework that shapes it all.
Regardless, the paper is a must-read:
Regardless, the paper is a must-read:
Reposted by Mathieu Lupien
Congratulations to this new generation of leading cancer scientists, and to the message they’re sending, with 50% of the awardees placing epigenetics at the heart of their research. The future is speaking. The path forward is both bright and bold.
July 30, 2025 at 4:18 AM
Congratulations to this new generation of leading cancer scientists, and to the message they’re sending, with 50% of the awardees placing epigenetics at the heart of their research. The future is speaking. The path forward is both bright and bold.
Nice study showing how DNA mutagenesis can be driven by transcription factor (TF) competition with mismatch repair machinery: www.cell.com/cell/fulltex...
Worth considering alongside the uneven mutagenesis of TF cistromes tied to their cancer-type-specific essentiality: www.cell.com/cancer-cell/...
Worth considering alongside the uneven mutagenesis of TF cistromes tied to their cancer-type-specific essentiality: www.cell.com/cancer-cell/...
DNA mutagenesis driven by transcription factor competition with mismatch repair
Competition between transcription factors and mismatch repair machinery drives localized
hypermutation at regulatory elements, with implications for cancer and genome evolution.
www.cell.com
July 30, 2025 at 2:15 PM
Nice study showing how DNA mutagenesis can be driven by transcription factor (TF) competition with mismatch repair machinery: www.cell.com/cell/fulltex...
Worth considering alongside the uneven mutagenesis of TF cistromes tied to their cancer-type-specific essentiality: www.cell.com/cancer-cell/...
Worth considering alongside the uneven mutagenesis of TF cistromes tied to their cancer-type-specific essentiality: www.cell.com/cancer-cell/...