Lucy Dornan
@lucygdornan.bsky.social
Cell biology PhD candidate & Golgi enthusiast with a fondness for live cell microscopy 🧫🔬
Nature lover, hobby baker, optimist ☀️
ucd.ie/hcs
Nature lover, hobby baker, optimist ☀️
ucd.ie/hcs
Managed to get two mammoth and problematic experiments working on the same day… and it’s a Sunday… suspicious… (everybody pray for colonies on my plates tomorrow)
spongebob squarepants from spongebob squarepants is holding a box on his shoulder .
ALT: spongebob squarepants from spongebob squarepants is holding a box on his shoulder .
media.tenor.com
November 23, 2025 at 1:26 PM
Managed to get two mammoth and problematic experiments working on the same day… and it’s a Sunday… suspicious… (everybody pray for colonies on my plates tomorrow)
Send help, I think I have met the cloning final boss of my PhD
November 12, 2025 at 9:00 AM
Send help, I think I have met the cloning final boss of my PhD
Happy halloween 🎃
This year’s pumpkin design is based on what occupies my thoughts and haunts me every minute of every day at the moment
This year’s pumpkin design is based on what occupies my thoughts and haunts me every minute of every day at the moment
October 31, 2025 at 1:27 PM
Happy halloween 🎃
This year’s pumpkin design is based on what occupies my thoughts and haunts me every minute of every day at the moment
This year’s pumpkin design is based on what occupies my thoughts and haunts me every minute of every day at the moment
statistically significant p values in a dataset that has taken two and a half years to produce
a man in a white shirt and black tie is standing in front of a scoreboard that says finals 2020 portugal
ALT: a man in a white shirt and black tie is standing in front of a scoreboard that says finals 2020 portugal
media.tenor.com
October 31, 2025 at 12:13 AM
statistically significant p values in a dataset that has taken two and a half years to produce
I jinxed myself SOOOOOO BADDDD with this
This week’s practical on our trusty confocal was rudely interrupted by a detector failure that took 1hr of the 2hr session to identify & resolve
Then as soon as it’s up & working again the gas detector in the room goes off :) three :) different :) times :)
This week’s practical on our trusty confocal was rudely interrupted by a detector failure that took 1hr of the 2hr session to identify & resolve
Then as soon as it’s up & working again the gas detector in the room goes off :) three :) different :) times :)
Feeling really reflective after 9 hours of microscopy practicals this week.. it is such a wonderful privilege to be able to show students fluorescence microscopy for the first time, there is nothing that can beat the feeling of joy at hearing all the “wow!”s when you first switch on the lasers 🥹
October 30, 2025 at 4:13 PM
I jinxed myself SOOOOOO BADDDD with this
This week’s practical on our trusty confocal was rudely interrupted by a detector failure that took 1hr of the 2hr session to identify & resolve
Then as soon as it’s up & working again the gas detector in the room goes off :) three :) different :) times :)
This week’s practical on our trusty confocal was rudely interrupted by a detector failure that took 1hr of the 2hr session to identify & resolve
Then as soon as it’s up & working again the gas detector in the room goes off :) three :) different :) times :)
One of these days I swear I am just going to come into the lab in my pyjamas
October 27, 2025 at 3:40 PM
One of these days I swear I am just going to come into the lab in my pyjamas
Feeling really reflective after 9 hours of microscopy practicals this week.. it is such a wonderful privilege to be able to show students fluorescence microscopy for the first time, there is nothing that can beat the feeling of joy at hearing all the “wow!”s when you first switch on the lasers 🥹
October 23, 2025 at 5:10 PM
Feeling really reflective after 9 hours of microscopy practicals this week.. it is such a wonderful privilege to be able to show students fluorescence microscopy for the first time, there is nothing that can beat the feeling of joy at hearing all the “wow!”s when you first switch on the lasers 🥹
Reposted by Lucy Dornan
🧠 The Lipid #Brain Atlas is out now! If you think #lipids are boring and membranes are all the same, prepare to be surprised. Led by @lucafusarbassini.bsky.social with Giovanni D'Angelo's lab, we mapped membrane lipids in the mouse brain at high resolution.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
October 16, 2025 at 6:23 AM
🧠 The Lipid #Brain Atlas is out now! If you think #lipids are boring and membranes are all the same, prepare to be surprised. Led by @lucafusarbassini.bsky.social with Giovanni D'Angelo's lab, we mapped membrane lipids in the mouse brain at high resolution.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
Reposted by Lucy Dornan
Attention membrane traffickers! I'm recruiting a post-doc to my lab in Dundee. We're dissecting the functions of intrinsically disordered domains of COPII coat proteins. We think they control timing of coat assembly and morphology of carriers.
www.dundee.ac.uk/work-for-us/...
www.dundee.ac.uk/work-for-us/...
Postdoctoral Research Assistant - MCDB - UOD2016
Closing date: Thursday 6 November 2025, 23:59
www.dundee.ac.uk
October 15, 2025 at 12:53 PM
Attention membrane traffickers! I'm recruiting a post-doc to my lab in Dundee. We're dissecting the functions of intrinsically disordered domains of COPII coat proteins. We think they control timing of coat assembly and morphology of carriers.
www.dundee.ac.uk/work-for-us/...
www.dundee.ac.uk/work-for-us/...
After many years of hm-ing and haw-ing about why some of the fusion proteins I’ve cloned showed cytoplasmic background I’ve finally found the culprit
October 10, 2025 at 7:51 AM
After many years of hm-ing and haw-ing about why some of the fusion proteins I’ve cloned showed cytoplasmic background I’ve finally found the culprit
Spending my weekend churning out data from live cell time series for a poster I have left very, very last minute; but at least the backdrop is pretty 🥹💞
September 27, 2025 at 7:19 PM
Spending my weekend churning out data from live cell time series for a poster I have left very, very last minute; but at least the backdrop is pretty 🥹💞
Late night live cell sesh, captured by @madeleenbrink.bsky.social 🫶
(featuring hair tied back by the chopped off end of a latex glove, life hack for all long hair havers who find themselves short of a hair tie in the lab)
(featuring hair tied back by the chopped off end of a latex glove, life hack for all long hair havers who find themselves short of a hair tie in the lab)
September 24, 2025 at 5:17 PM
Late night live cell sesh, captured by @madeleenbrink.bsky.social 🫶
(featuring hair tied back by the chopped off end of a latex glove, life hack for all long hair havers who find themselves short of a hair tie in the lab)
(featuring hair tied back by the chopped off end of a latex glove, life hack for all long hair havers who find themselves short of a hair tie in the lab)
How does a cell just fit that much stuff inside of it . Like there is SO MUCH jammed into something SO small .
September 24, 2025 at 3:24 PM
How does a cell just fit that much stuff inside of it . Like there is SO MUCH jammed into something SO small .
Just finished imaging my last rep of an experiment that has taken a grand total of 6100 minutes (101.66 hrs) of live-cell confocal imaging, spread across 305x 35mm dishes
September 11, 2025 at 3:30 PM
Just finished imaging my last rep of an experiment that has taken a grand total of 6100 minutes (101.66 hrs) of live-cell confocal imaging, spread across 305x 35mm dishes
Went outside to go look at the stars and all I can see are Starlink satellites . :(
September 4, 2025 at 9:08 PM
Went outside to go look at the stars and all I can see are Starlink satellites . :(
Reposted by Lucy Dornan
If you have an exciting microscopy-based membrane biology discovery to showcase this may be for you! Submit your abstracts and/or get in touch! Please repost
Last call for applications for ECRs to speak at our #FocalPlaneFeatures symposium co-hosted with the #MembraneTrafficking webinar series organised by @franbottanelli.bsky.social @felixmendu.bsky.social & @ishier.bsky.social.
Apply by 24 August
focalplane.biologists.com/2025/07/14/f...
Apply by 24 August
focalplane.biologists.com/2025/07/14/f...
FocalPlane features... membrane trafficking - apply to give a talk - FocalPlane
FocalPlane features... membrane trafficking - apply to give a talk - News
focalplane.biologists.com
August 20, 2025 at 12:07 PM
If you have an exciting microscopy-based membrane biology discovery to showcase this may be for you! Submit your abstracts and/or get in touch! Please repost
A brief moment of solitude and reflection beneath the trees today :)
After 7 years in UCD between my undergrad and the PhD, today I finished my last ever accredited module. Feeling so grateful for the opportunity to have learnt so much about myself and the world, and excited for the future :)
After 7 years in UCD between my undergrad and the PhD, today I finished my last ever accredited module. Feeling so grateful for the opportunity to have learnt so much about myself and the world, and excited for the future :)
August 15, 2025 at 2:37 PM
A brief moment of solitude and reflection beneath the trees today :)
After 7 years in UCD between my undergrad and the PhD, today I finished my last ever accredited module. Feeling so grateful for the opportunity to have learnt so much about myself and the world, and excited for the future :)
After 7 years in UCD between my undergrad and the PhD, today I finished my last ever accredited module. Feeling so grateful for the opportunity to have learnt so much about myself and the world, and excited for the future :)
Reposted by Lucy Dornan
Taking a shot here, a colleague is seeking a #postdoc position in/near #Germany. She’s eager to expand into #organoids , & has experience in #Spheroids, #BiologicalImaging, #CellBiology #HighContentMicroscopy
Any leads, connections, or suggestions would be hugely appreciated!
#Microscopy
Any leads, connections, or suggestions would be hugely appreciated!
#Microscopy
August 14, 2025 at 1:11 PM
Taking a shot here, a colleague is seeking a #postdoc position in/near #Germany. She’s eager to expand into #organoids , & has experience in #Spheroids, #BiologicalImaging, #CellBiology #HighContentMicroscopy
Any leads, connections, or suggestions would be hugely appreciated!
#Microscopy
Any leads, connections, or suggestions would be hugely appreciated!
#Microscopy
Reposted by Lucy Dornan
Excited to share our new @pubs.acs.org paper! We engineered cells with ~10% photolipids in the ER membrane. This enabled optical control of membrane viscosity to study its impact on ER→Golgi protein transport. @dirktrauner.bsky.social @noemijimenezrojo.bsky.social
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Optical Control of Membrane Viscosity Modulates ER-to-Golgi Trafficking
The lipid composition of cellular membranes is highly dynamic and undergoes continuous remodeling, affecting the biophysical properties critical to biological function. Here, we introduce an optical a...
pubs.acs.org
August 14, 2025 at 10:43 AM
Excited to share our new @pubs.acs.org paper! We engineered cells with ~10% photolipids in the ER membrane. This enabled optical control of membrane viscosity to study its impact on ER→Golgi protein transport. @dirktrauner.bsky.social @noemijimenezrojo.bsky.social
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Just out of curiosity… who here also maintains an active LinkedIn profile?
August 14, 2025 at 11:33 AM
Just out of curiosity… who here also maintains an active LinkedIn profile?
Reposted by Lucy Dornan
Our protocol for gene editing is online and all plasmids are on @addgene.bsky.social! CRISPR away everybody and get in touch if you need anything 🤩
Protocol for fast antibiotic resistance-based gene editing of mammalian cells with CRISPR-Cas9 #protocol #starprotocols #cellpress
Protocol for fast antibiotic resistance-based gene editing of mammalian cells with CRISPR-Cas9
Protein tagging with CRISPR-Cas9 enables the investigation of protein function in its native environment but is limited by low homology-directed repair (HDR) efficiency. Here, we present a protocol for fast antibiotic resistance-based gene editing with CRISPR-Cas9 (FAB-CRISPR), which streamlines N/C-terminal tagging using an antibiotic resistance cassette for rapid selection and enrichment of gene-edited cells. We describe in detail guide RNA and HDR donor plasmid cloning, transfection of editin...
dlvr.it
August 13, 2025 at 10:22 AM
Our protocol for gene editing is online and all plasmids are on @addgene.bsky.social! CRISPR away everybody and get in touch if you need anything 🤩
Going through a lot of flip flopping between psyching and hyping myself up to really knuckle tf down for final year, and then just staring at my to-do list and calendar like this
a woman with curly hair is sitting at a desk with her hands on her head and a hulu logo in the corner
ALT: a woman with curly hair is sitting at a desk with her hands on her head and a hulu logo in the corner
media.tenor.com
August 12, 2025 at 4:33 PM
Going through a lot of flip flopping between psyching and hyping myself up to really knuckle tf down for final year, and then just staring at my to-do list and calendar like this
Personally I think that scientists should invent a pair of goggles that lets you see every single molecular interaction in a single cell at once
August 8, 2025 at 5:03 PM
Personally I think that scientists should invent a pair of goggles that lets you see every single molecular interaction in a single cell at once
Reposted by Lucy Dornan
Microscopy most especially. To be the first human to ever lay eyes on something. What a feeling.
Really just had something like this feeling this afternoon. It’s the high that keeps you coming back for more
August 8, 2025 at 1:32 AM
Microscopy most especially. To be the first human to ever lay eyes on something. What a feeling.