Loyal Goff
@loyalgoff.bsky.social
I study neural development and cell fate commitment in cephalopods (and sometimes mammals) @ Johns Hopkins. Nice even split between computational and molecular biology.
Reposted by Loyal Goff
A preprint‼️that's bound to ruffle some 🪶 "Widespread DNA off-targeting confounds studies of RNA chromatin occupancy" led by our Micah Goldrich and Louis Delhaye from
Pieter Mestdagh in Ghent. TL;DR we show that many of lncRNA chromatin occupancy obtained are flawed www.biorxiv.org/content/10.1...
Pieter Mestdagh in Ghent. TL;DR we show that many of lncRNA chromatin occupancy obtained are flawed www.biorxiv.org/content/10.1...
November 13, 2025 at 9:26 AM
A preprint‼️that's bound to ruffle some 🪶 "Widespread DNA off-targeting confounds studies of RNA chromatin occupancy" led by our Micah Goldrich and Louis Delhaye from
Pieter Mestdagh in Ghent. TL;DR we show that many of lncRNA chromatin occupancy obtained are flawed www.biorxiv.org/content/10.1...
Pieter Mestdagh in Ghent. TL;DR we show that many of lncRNA chromatin occupancy obtained are flawed www.biorxiv.org/content/10.1...
Reposted by Loyal Goff
Yeah, I no longer have ESI status due to collaborative work, but because I didn't get an ESI MIRA first, I'm probably locked out of a MIRA forever, because only unicorns get NIGMS R01s these days. The "must have an NIGMS R01" requirement might have worked at the start, but definitely doesn't now.
September 30, 2025 at 2:58 PM
Yeah, I no longer have ESI status due to collaborative work, but because I didn't get an ESI MIRA first, I'm probably locked out of a MIRA forever, because only unicorns get NIGMS R01s these days. The "must have an NIGMS R01" requirement might have worked at the start, but definitely doesn't now.
Reposted by Loyal Goff
On the nature of a NSF (or NIH) graduate training fellowship drugmonkey.wordpress.com/2025/09/29/o...
On the nature of a NSF (or NIH) graduate training fellowship
I had a thought about graduate school fellowships and added it to the prior past about the NSF GRFP limitations to first year graduate students. [ETA 09/29/2025: I forgot to mention something impor…
drugmonkey.wordpress.com
September 30, 2025 at 1:28 AM
On the nature of a NSF (or NIH) graduate training fellowship drugmonkey.wordpress.com/2025/09/29/o...
Reposted by Loyal Goff
Alfonso-Gonzalez, C., Shi, M., Gorey, S., Holec, S., Carrasco, J., Rauer, M., Tsagkris, S., Mateos, F., Hilgers, V. (2025). ELAV mediates circular RNA biogenesis in neurons. Genes Dev, genesdev.cshlp.org/content/earl...
ELAV mediates circular RNA biogenesis in neurons
A biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields
genesdev.cshlp.org
August 27, 2025 at 11:10 PM
Alfonso-Gonzalez, C., Shi, M., Gorey, S., Holec, S., Carrasco, J., Rauer, M., Tsagkris, S., Mateos, F., Hilgers, V. (2025). ELAV mediates circular RNA biogenesis in neurons. Genes Dev, genesdev.cshlp.org/content/earl...
A few weeks ago, this beautiful and inspired mural was painted on the building behind my lab space at Johns Hopkins. I walk past it each day and honestly feel humbled by how it makes me feel. Feeling compelled to share it with others today.
August 26, 2025 at 2:27 PM
A few weeks ago, this beautiful and inspired mural was painted on the building behind my lab space at Johns Hopkins. I walk past it each day and honestly feel humbled by how it makes me feel. Feeling compelled to share it with others today.
Reposted by Loyal Goff
If you are someone actively involved in cephalopod research whether you are a Masters student, PhD student, Postdoc, Professor, Emeritus, reach out to me via the Bsky chat so we can get you invited to the Cephalopod Research Network Discord server! It's essentially a year-round CIAC!
August 16, 2025 at 7:43 AM
If you are someone actively involved in cephalopod research whether you are a Masters student, PhD student, Postdoc, Professor, Emeritus, reach out to me via the Bsky chat so we can get you invited to the Cephalopod Research Network Discord server! It's essentially a year-round CIAC!
Reposted by Loyal Goff
Reposted by Loyal Goff
With more mollusk genomes available, a new genome-based phylogeny is featured on the cover of Science. Paper from @sigwartae.bsky.social at www.science.org/doi/10.1126/... Cover photo by Magnus Lundgren.
February 28, 2025 at 12:47 PM
With more mollusk genomes available, a new genome-based phylogeny is featured on the cover of Science. Paper from @sigwartae.bsky.social at www.science.org/doi/10.1126/... Cover photo by Magnus Lundgren.
Reposted by Loyal Goff
A mosquito cell atlas www.biorxiv.org/content/10.1... ht @leslievosshall.bsky.social
Mosquito Cell Atlas: A single-nucleus transcriptomic atlas of the adult Aedes aegypti mosquito
The female mosquito's remarkable ability to hunt humans and transmit pathogens relies on her unique biology. Here, we present the Mosquito Cell Atlas (MCA), a comprehensive single-nucleus RNA sequenci...
www.biorxiv.org
February 26, 2025 at 4:07 PM
A mosquito cell atlas www.biorxiv.org/content/10.1... ht @leslievosshall.bsky.social
Reposted by Loyal Goff
Reposted by Loyal Goff
'Trans-differentiation of neutrophils from plasmablast' is an artefact caused by over-reliance on machine algorithms in single cell RNA sequencing analysis: Lesson learnt and steps ahead https://www.biorxiv.org/content/10.1101/2025.02.07.636761v1
February 8, 2025 at 1:53 AM
'Trans-differentiation of neutrophils from plasmablast' is an artefact caused by over-reliance on machine algorithms in single cell RNA sequencing analysis: Lesson learnt and steps ahead https://www.biorxiv.org/content/10.1101/2025.02.07.636761v1
Reposted by Loyal Goff
Just saw this. NIH is capping indirects at 15%. This will crush so many institutions. grants.nih.gov/grants/guide...
NOT-OD-25-068: Supplemental Guidance to the 2024 NIH Grants Policy Statement: Indirect Cost Rates
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: Supplemental Guidance to the 2024 NIH Grants Policy Statement: Indirect Cost Rates NOT-OD-25-068. OD
grants.nih.gov
February 8, 2025 at 12:19 AM
Just saw this. NIH is capping indirects at 15%. This will crush so many institutions. grants.nih.gov/grants/guide...
Reposted by Loyal Goff
70% of scientists say they experience the imposter syndrome. I still experience it too sometimes, and what helps is talking with a science buddy with whom it’s ok to say stupid things, which may actually turn out to be good ideas.
November 24, 2024 at 3:07 AM
70% of scientists say they experience the imposter syndrome. I still experience it too sometimes, and what helps is talking with a science buddy with whom it’s ok to say stupid things, which may actually turn out to be good ideas.
Reposted by Loyal Goff
New method for identifying spatially variable genes in spatial transcriptomics.
We use spectral graph theory to extract 1D curves related to tissue morphology, creating a more relevant coordinate system. Improves sensitivity.
with @PhillipNicol et al.
www.biorxiv.org/content/10.1...
We use spectral graph theory to extract 1D curves related to tissue morphology, creating a more relevant coordinate system. Improves sensitivity.
with @PhillipNicol et al.
www.biorxiv.org/content/10.1...
November 22, 2024 at 8:04 PM
New method for identifying spatially variable genes in spatial transcriptomics.
We use spectral graph theory to extract 1D curves related to tissue morphology, creating a more relevant coordinate system. Improves sensitivity.
with @PhillipNicol et al.
www.biorxiv.org/content/10.1...
We use spectral graph theory to extract 1D curves related to tissue morphology, creating a more relevant coordinate system. Improves sensitivity.
with @PhillipNicol et al.
www.biorxiv.org/content/10.1...
Reposted by Loyal Goff
Nice work from Nicole Lake and Monkol Lek (with a small contribution from our group):
medicalxpress.com/news/2024-11...
medicalxpress.com/news/2024-11...
Decoding disease: Novel tool helps identify mutations in mitochondrial DNA
Geneticists looking inside the nuclear genome for mutations that contribute to disease have long relied on a principal known as constraint modeling, which allows researchers to assess the degree of se...
medicalxpress.com
November 15, 2024 at 1:39 PM
Nice work from Nicole Lake and Monkol Lek (with a small contribution from our group):
medicalxpress.com/news/2024-11...
medicalxpress.com/news/2024-11...