Kristin Knouse
@kristinknouse.bsky.social
Whitehead Career Development Professor at MIT Department of Biology and Koch Institute for Cancer Research. Building tools to understand and modulate regenerative capacity across tissues.
Reposted by Kristin Knouse
ACOG reaffirms that acetaminophen is safe for managing pain and fever during pregnancy. No reputable studies support suggestions like those in HHS’s recent announcement linking acetaminophen use in pregnancy to autism; in fact, high-quality studies show no such risk. https://bit.ly/47Wxc59
September 22, 2025 at 9:24 PM
ACOG reaffirms that acetaminophen is safe for managing pain and fever during pregnancy. No reputable studies support suggestions like those in HHS’s recent announcement linking acetaminophen use in pregnancy to autism; in fact, high-quality studies show no such risk. https://bit.ly/47Wxc59
Reposted by Kristin Knouse
The Garnett Lab is excited to launch DepMap Miner, a new tool to explore cancer dependency data!
Built on the Sanger DepMap, the DepMap Miner brings together multi-omic and functional datasets for over 1300 cancer cell models.
Start mining data now: dataminer.depmap.sanger.ac.uk
Built on the Sanger DepMap, the DepMap Miner brings together multi-omic and functional datasets for over 1300 cancer cell models.
Start mining data now: dataminer.depmap.sanger.ac.uk
September 20, 2025 at 7:43 AM
The Garnett Lab is excited to launch DepMap Miner, a new tool to explore cancer dependency data!
Built on the Sanger DepMap, the DepMap Miner brings together multi-omic and functional datasets for over 1300 cancer cell models.
Start mining data now: dataminer.depmap.sanger.ac.uk
Built on the Sanger DepMap, the DepMap Miner brings together multi-omic and functional datasets for over 1300 cancer cell models.
Start mining data now: dataminer.depmap.sanger.ac.uk
Reposted by Kristin Knouse
New preprint! Graduate student Océane Marescal leverages quiescence - proliferative hibernation - to reveal unexpected dynamics for “constitutively”-localized centromere proteins. To understand the logic of cell division, you need to consider non-dividing cells.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
The dynamics of centromere assembly and disassembly during quiescence
Quiescence is a state in which cells undergo a prolonged proliferative arrest while maintaining their capacity to reenter the cell cycle. Here, we analyze entry and exit from quiescence, focusing on h...
www.biorxiv.org
September 9, 2025 at 11:14 AM
New preprint! Graduate student Océane Marescal leverages quiescence - proliferative hibernation - to reveal unexpected dynamics for “constitutively”-localized centromere proteins. To understand the logic of cell division, you need to consider non-dividing cells.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Kristin Knouse
Doomscrolling pause to chat CRISPR libraries! Preprint describes our new, data-driven approach to combine on-target and off-target predictions much more intelligently for *selecting* guides, which we use to develop our newest Cas9 knockout library, Jacquere. Thread: www.biorxiv.org/content/10.1...
Balancing off-target and on-target considerations for optimized Cas9 CRISPR knockout library design
The continued development of high-dimensional CRISPR screen readouts, such as single-cell RNA sequencing and high-content imaging, necessitates compact libraries to enable functional interrogation at ...
www.biorxiv.org
September 7, 2025 at 5:02 PM
Doomscrolling pause to chat CRISPR libraries! Preprint describes our new, data-driven approach to combine on-target and off-target predictions much more intelligently for *selecting* guides, which we use to develop our newest Cas9 knockout library, Jacquere. Thread: www.biorxiv.org/content/10.1...
Lab retreat 2025 was a reel good time!
September 2, 2025 at 12:01 PM
Lab retreat 2025 was a reel good time!
Reposted by Kristin Knouse
Congrats to Sourav Ghosh (@souravsgw.bsky.social) of IIT Bombay
& Kotaro Tomuro of RIKEN and The University of Tokyo - our 2025 Amon Young Scientist Award winners! They present Nov 6, 10 a.m. at the Koch Institute. MIT community & Amon Lab alumni welcome. buff.ly/nzhbwPp
& Kotaro Tomuro of RIKEN and The University of Tokyo - our 2025 Amon Young Scientist Award winners! They present Nov 6, 10 a.m. at the Koch Institute. MIT community & Amon Lab alumni welcome. buff.ly/nzhbwPp
August 14, 2025 at 4:11 PM
Congrats to Sourav Ghosh (@souravsgw.bsky.social) of IIT Bombay
& Kotaro Tomuro of RIKEN and The University of Tokyo - our 2025 Amon Young Scientist Award winners! They present Nov 6, 10 a.m. at the Koch Institute. MIT community & Amon Lab alumni welcome. buff.ly/nzhbwPp
& Kotaro Tomuro of RIKEN and The University of Tokyo - our 2025 Amon Young Scientist Award winners! They present Nov 6, 10 a.m. at the Koch Institute. MIT community & Amon Lab alumni welcome. buff.ly/nzhbwPp
Our liver can completely regenerate itself in a matter of days! Excited to share this perspective on how the liver breaks the rules of cell biology to accomplish this exceptional feat and how this could unlock regenerative capacity in other organs.
www.molbiolcell.org/doi/10.1091/...
www.molbiolcell.org/doi/10.1091/...
Breaking the rules of cell biology: Lessons from the liver's exceptional regenerative capacity | Molecular Biology of the Cell
The inability of most human organs to regenerate themselves after injury underlies the lifelong morbidity of numerous diseases. As we continue to seek solutions for these intractable conditions, the l...
www.molbiolcell.org
May 29, 2025 at 12:11 PM
Our liver can completely regenerate itself in a matter of days! Excited to share this perspective on how the liver breaks the rules of cell biology to accomplish this exceptional feat and how this could unlock regenerative capacity in other organs.
www.molbiolcell.org/doi/10.1091/...
www.molbiolcell.org/doi/10.1091/...
Reposted by Kristin Knouse
A pie graph worth keeping in mind as the NIH budget plummets jamanetwork.com/journals/jam... for 356 new FDA drugs approved
March 23, 2025 at 4:17 PM
A pie graph worth keeping in mind as the NIH budget plummets jamanetwork.com/journals/jam... for 356 new FDA drugs approved
Reposted by Kristin Knouse
Excited to share the work of Jonathan Perr where he uncovered a surprisingly common feature of cell surfaces - the presentation and clustering of RNA binding proteins with #glycoRNA.
Critically support by #NIH @cp-cell.bsky.social www.cell.com/cell/abstrac...
Critically support by #NIH @cp-cell.bsky.social www.cell.com/cell/abstrac...
RNA-binding proteins and glycoRNAs form domains on the cell surface for cell-penetrating peptide entry
Mammalian cells present RNA-binding proteins on the cell surface that form clustered
domains containing glycoRNAs.
www.cell.com
February 27, 2025 at 3:48 PM
Excited to share the work of Jonathan Perr where he uncovered a surprisingly common feature of cell surfaces - the presentation and clustering of RNA binding proteins with #glycoRNA.
Critically support by #NIH @cp-cell.bsky.social www.cell.com/cell/abstrac...
Critically support by #NIH @cp-cell.bsky.social www.cell.com/cell/abstrac...
Reposted by Kristin Knouse
Excited to share the peer-reviewed version of our paper on predicting the chromatin response to TF dosage using transfer learning
www.cell.com/cell-genomic...
www.cell.com/cell-genomic...
Transfer learning reveals sequence determinants of the quantitative response to transcription factor dosage
Naqvi et al. reveal how DNA sequence determines the chromatin response to transcription
factor (TF) dosage changes. By combining deep learning and chemical genetics, they
uncover specific sequence fea...
www.cell.com
February 27, 2025 at 8:31 PM
Excited to share the peer-reviewed version of our paper on predicting the chromatin response to TF dosage using transfer learning
www.cell.com/cell-genomic...
www.cell.com/cell-genomic...
Can we perform genome-wide screens in any cell type in the body?! Excited to share our roadmap for leveraging advances in sgRNA delivery, library design, and phenotypic selection to enable unprecedented genetic dissection of organismal physiology and disease.
www.cell.com/cell-genomic...
www.cell.com/cell-genomic...
A roadmap toward genome-wide CRISPR screening throughout the organism
Genome-wide CRISPR screening in the organism has tremendous potential to answer long-standing
questions of physiology and disease; however, technical limitations have prevented
its broad application. ...
www.cell.com
February 24, 2025 at 4:08 PM
Can we perform genome-wide screens in any cell type in the body?! Excited to share our roadmap for leveraging advances in sgRNA delivery, library design, and phenotypic selection to enable unprecedented genetic dissection of organismal physiology and disease.
www.cell.com/cell-genomic...
www.cell.com/cell-genomic...
Reposted by Kristin Knouse
Here is a quick thread to promote my research! After years of dedicated research, I'm thrilled to share our article just published at Nature Communications :)
www.nature.com/articles/s41...
#metabolism #CRISPRscreens #mitochondria
www.nature.com/articles/s41...
#metabolism #CRISPRscreens #mitochondria
SLC25A38 is required for mitochondrial pyridoxal 5’-phosphate (PLP) accumulation
Nature Communications - Pyridoxal 5’-phosphate (PLP, vitamin B6) is crucial for various metabolic processes. Here, the authors identified SLC25A38 as a key regulator of mitochondrial PLP...
www.nature.com
January 25, 2025 at 12:29 PM
Here is a quick thread to promote my research! After years of dedicated research, I'm thrilled to share our article just published at Nature Communications :)
www.nature.com/articles/s41...
#metabolism #CRISPRscreens #mitochondria
www.nature.com/articles/s41...
#metabolism #CRISPRscreens #mitochondria
Reposted by Kristin Knouse
Viral Vectors can be difficult to use, but Addgene's Viral Vectors 101 eBook can help! Our second edition has helpful content, useful images, and increased accessibility for all. Find it here: www.addgene.org/educ...
January 15, 2025 at 1:15 PM
Viral Vectors can be difficult to use, but Addgene's Viral Vectors 101 eBook can help! Our second edition has helpful content, useful images, and increased accessibility for all. Find it here: www.addgene.org/educ...
Reposted by Kristin Knouse
Preprint alert!! How zonated hepatocytes adapt to changes in lipid influx due to fasting or a Western diet (WD). We developed a microscopy-based phenotyping method called single-cell phenomics (scPhenomics) to measure the features of mitochondria and lipid droplets (LDs).
December 12, 2024 at 5:11 PM
Preprint alert!! How zonated hepatocytes adapt to changes in lipid influx due to fasting or a Western diet (WD). We developed a microscopy-based phenotyping method called single-cell phenomics (scPhenomics) to measure the features of mitochondria and lipid droplets (LDs).
Reposted by Kristin Knouse
How did we get here, with a prominent anti-vaxxer nominated to lead Health and Human Services, with its $1.7T budget?
Much of it can be traced to this retracted 1998 paper, falsely linking the measles, mumps and rubella (MMR) vaccine to autism. 1/3
Much of it can be traced to this retracted 1998 paper, falsely linking the measles, mumps and rubella (MMR) vaccine to autism. 1/3
November 30, 2024 at 2:05 AM
How did we get here, with a prominent anti-vaxxer nominated to lead Health and Human Services, with its $1.7T budget?
Much of it can be traced to this retracted 1998 paper, falsely linking the measles, mumps and rubella (MMR) vaccine to autism. 1/3
Much of it can be traced to this retracted 1998 paper, falsely linking the measles, mumps and rubella (MMR) vaccine to autism. 1/3
Reposted by Kristin Knouse
Join us for the 6th CRISPR and Beyond Conference on 2-4 April 2025!🧬 A must-attend event for researchers in high-throughput screening and #GeneEditing, exploring emerging technologies and models. #CRISPR25
👉Programme and registration: bit.ly/3Amz34N
👉Programme and registration: bit.ly/3Amz34N
November 19, 2024 at 2:40 PM
Join us for the 6th CRISPR and Beyond Conference on 2-4 April 2025!🧬 A must-attend event for researchers in high-throughput screening and #GeneEditing, exploring emerging technologies and models. #CRISPR25
👉Programme and registration: bit.ly/3Amz34N
👉Programme and registration: bit.ly/3Amz34N
Reposted by Kristin Knouse
If you don't fit in, that could actually be your biggest advantage for having new ideas. ADHD, dyslexia, narcolepsy, etc.. anything that makes you different is of tremendous value when it comes to being creative.
open.spotify.com/episode/1b1k...
open.spotify.com/episode/1b1k...
57 | George Church and shooting for the stars
Night Science · Episode
open.spotify.com
November 14, 2024 at 1:48 PM
If you don't fit in, that could actually be your biggest advantage for having new ideas. ADHD, dyslexia, narcolepsy, etc.. anything that makes you different is of tremendous value when it comes to being creative.
open.spotify.com/episode/1b1k...
open.spotify.com/episode/1b1k...
We are privileged to continue honoring Angelika’s inspiring legacy of mentorship and discovery with the third annual Angelika Amon Young Scientist Award! Please encourage PhD students outside the US who embody her infectious passion for biology to apply!
ki.mit.edu/events/prize...
ki.mit.edu/events/prize...
February 12, 2024 at 11:32 AM
We are privileged to continue honoring Angelika’s inspiring legacy of mentorship and discovery with the third annual Angelika Amon Young Scientist Award! Please encourage PhD students outside the US who embody her infectious passion for biology to apply!
ki.mit.edu/events/prize...
ki.mit.edu/events/prize...
“Underlying all the conversations…the most unanswerable question of all: Would a man have been treated the same way?”
Thank you @kzernike for this important and timely piece highlighting the biases faced by women and other underrepresented leaders.
www.nytimes.com/2024/01/28/u...
Thank you @kzernike for this important and timely piece highlighting the biases faced by women and other underrepresented leaders.
www.nytimes.com/2024/01/28/u...
The Campus Wars Aren’t About Gender … Are They?
Recent Ivy League dramas have made women leaders in academia wonder how far they’ve really come.
www.nytimes.com
January 28, 2024 at 4:06 PM
“Underlying all the conversations…the most unanswerable question of all: Would a man have been treated the same way?”
Thank you @kzernike for this important and timely piece highlighting the biases faced by women and other underrepresented leaders.
www.nytimes.com/2024/01/28/u...
Thank you @kzernike for this important and timely piece highlighting the biases faced by women and other underrepresented leaders.
www.nytimes.com/2024/01/28/u...
Happy Halloween from the Knouse Lab, where we’re always down for a good mystery!
October 31, 2023 at 12:04 PM
Happy Halloween from the Knouse Lab, where we’re always down for a good mystery!