Dipak Kathyat
@kathayatdipak.bsky.social
Assistant Professor of Microbiology, Richard A. Gillespie College of Veterinary Medicine, Bacterial physiology and pathogenesis, Drug discovery, Antibiotic resistance, #BuckeyeForLife
Harrogate, TN
https://pubmed.ncbi.nlm.nih.gov/?term=kathayat+D&sort=date
Harrogate, TN
https://pubmed.ncbi.nlm.nih.gov/?term=kathayat+D&sort=date
Reposted by Dipak Kathyat
The diderm cell envelope is not a stack of layers but a unified scaffold of Inner Membrane–Peptidoglycan–Outer Membrane.
We discuss how tethering the OM to the PG in E. coli preserves integrity — and extend the concept across diderm bacteria.
Curr Opin Microbiol: doi.org/10.1016/j.mi...
#microsky 🔬
We discuss how tethering the OM to the PG in E. coli preserves integrity — and extend the concept across diderm bacteria.
Curr Opin Microbiol: doi.org/10.1016/j.mi...
#microsky 🔬
Redirecting
doi.org
November 13, 2025 at 12:17 PM
The diderm cell envelope is not a stack of layers but a unified scaffold of Inner Membrane–Peptidoglycan–Outer Membrane.
We discuss how tethering the OM to the PG in E. coli preserves integrity — and extend the concept across diderm bacteria.
Curr Opin Microbiol: doi.org/10.1016/j.mi...
#microsky 🔬
We discuss how tethering the OM to the PG in E. coli preserves integrity — and extend the concept across diderm bacteria.
Curr Opin Microbiol: doi.org/10.1016/j.mi...
#microsky 🔬
Reposted by Dipak Kathyat
Delighted to share our latest paper - A host-directed adjuvant sensitizes intracellular bacterial persisters to antibiotics. Excellently led by Dr. Kuan-Yi Lu. We think it's a great proof-of-concept that altering immune cell behavior can make antibiotics work better www.nature.com/articles/s41...
A host-directed adjuvant sensitizes intracellular bacterial persisters to antibiotics - Nature Microbiology
The authors developed a screen to find compounds that modulate intracellular Staphylococcus aureus metabolism and discovered KL1, which sensitizes persisters to antibiotics by reversing host-induced tolerance.
www.nature.com
October 10, 2025 at 1:24 PM
Delighted to share our latest paper - A host-directed adjuvant sensitizes intracellular bacterial persisters to antibiotics. Excellently led by Dr. Kuan-Yi Lu. We think it's a great proof-of-concept that altering immune cell behavior can make antibiotics work better www.nature.com/articles/s41...
I am very happy to share that I have started this week as an Assistant Professor of Microbiology at the Richard A. Gillespie College of Veterinary Medicine in Harrogate, Tennessee. It has been a beautiful journey from IAAS to Ohio State to Cornell, and now to LMU-CVM.
October 3, 2025 at 7:43 AM
I am very happy to share that I have started this week as an Assistant Professor of Microbiology at the Richard A. Gillespie College of Veterinary Medicine in Harrogate, Tennessee. It has been a beautiful journey from IAAS to Ohio State to Cornell, and now to LMU-CVM.
Reposted by Dipak Kathyat
We discovered a new family of c-di-NMP receptors, widespread in bacteria & regulating🦠motility @pnas.org
A great collab. w/ @mygalperin.bsky.social @vikramalva.bsky.social @thethormannden.bsky.social
www.pnas.org/doi/10.1073/...
@hhu.de @cmfi.bsky.social
@sfb1381.bsky.social
@mibinet.bsky.social
A great collab. w/ @mygalperin.bsky.social @vikramalva.bsky.social @thethormannden.bsky.social
www.pnas.org/doi/10.1073/...
@hhu.de @cmfi.bsky.social
@sfb1381.bsky.social
@mibinet.bsky.social
September 19, 2025 at 2:32 PM
We discovered a new family of c-di-NMP receptors, widespread in bacteria & regulating🦠motility @pnas.org
A great collab. w/ @mygalperin.bsky.social @vikramalva.bsky.social @thethormannden.bsky.social
www.pnas.org/doi/10.1073/...
@hhu.de @cmfi.bsky.social
@sfb1381.bsky.social
@mibinet.bsky.social
A great collab. w/ @mygalperin.bsky.social @vikramalva.bsky.social @thethormannden.bsky.social
www.pnas.org/doi/10.1073/...
@hhu.de @cmfi.bsky.social
@sfb1381.bsky.social
@mibinet.bsky.social
Reposted by Dipak Kathyat
The outer membrane of Gram-negative bacteria blocks many antibiotics. Our latest work reveals that L-type pyocins bypass this barrier by inactivating the BAM complex, killing Pseudomonas aeruginosa without entering the cell, providing a new blueprint for beating antibiotic resistance.
A Protein Antibiotic Inhibits the BAM Complex to Kill Without Cell Entry
Many antibiotics are ineffective against Gram-negative pathogens such as Pseudomonas aeruginosa because they cannot penetrate the bacterial outer membrane. Here, we show that protein antibiotics calle...
www.biorxiv.org
September 20, 2025 at 9:29 AM
The outer membrane of Gram-negative bacteria blocks many antibiotics. Our latest work reveals that L-type pyocins bypass this barrier by inactivating the BAM complex, killing Pseudomonas aeruginosa without entering the cell, providing a new blueprint for beating antibiotic resistance.
Reposted by Dipak Kathyat
Latest from the lab! Analysis of everyone’s favorite regulatory mechanism in bacteria — the RF2 programmed frameshift! Likely present in the ancestor of bacteria, use of this mechanism is influenced by stop codon usage! Big congrats to @cassidyprints.bsky.social
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
Conservation and evolution of the programmed ribosomal frameshift in prfB across the bacterial domain | mBio
Translation termination is catalyzed by one of two release factors in bacteria, RF1 or RF2. It has been known for decades that RF2 levels in Escherichia coli are regulated by a programmed ribosomal frameshift within the prfB gene that encodes RF2. We ...
journals.asm.org
August 19, 2025 at 7:44 PM
Latest from the lab! Analysis of everyone’s favorite regulatory mechanism in bacteria — the RF2 programmed frameshift! Likely present in the ancestor of bacteria, use of this mechanism is influenced by stop codon usage! Big congrats to @cassidyprints.bsky.social
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
Reposted by Dipak Kathyat
#microsky
We challenge the long-standing view that peptidoglycan alone protects cells from bursting.
Our study shows that the periplasm — enclosed by OM–PG connections — acts as a pressure buffer essential for osmoprotection in Gram-negative bacteria.
📄 www.nature.com/articles/s41...
We challenge the long-standing view that peptidoglycan alone protects cells from bursting.
Our study shows that the periplasm — enclosed by OM–PG connections — acts as a pressure buffer essential for osmoprotection in Gram-negative bacteria.
📄 www.nature.com/articles/s41...
Peptidoglycan–outer membrane attachment generates periplasmic pressure to prevent lysis in Gram-negative bacteria - Nature Microbiology
Outer membrane attachment to peptidoglycan enables periplasmic pressure to build up and counter cytoplasmic turgor pressure, preventing lysis during osmotic challenges in Escherichia coli.
www.nature.com
July 29, 2025 at 1:23 PM
#microsky
We challenge the long-standing view that peptidoglycan alone protects cells from bursting.
Our study shows that the periplasm — enclosed by OM–PG connections — acts as a pressure buffer essential for osmoprotection in Gram-negative bacteria.
📄 www.nature.com/articles/s41...
We challenge the long-standing view that peptidoglycan alone protects cells from bursting.
Our study shows that the periplasm — enclosed by OM–PG connections — acts as a pressure buffer essential for osmoprotection in Gram-negative bacteria.
📄 www.nature.com/articles/s41...
It is a dream-come-true moment for me! The unique long-chain fatty acid transporter of Legionella pneumophila that we reported in our earlier study made it to the JB cover!
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
March 20, 2025 at 10:47 PM
It is a dream-come-true moment for me! The unique long-chain fatty acid transporter of Legionella pneumophila that we reported in our earlier study made it to the JB cover!
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
Reposted by Dipak Kathyat
A dual-target antibiotic strategy, combining membrane disruption with another cellular pathway, shows promise in reducing bacterial resistance, offering a new direction in combating antimicrobial resistance. doi.org/g855sh
Study uncovers the core principles of low-resistance antibiotics
A study published in Nature Communications has revealed novel insights into bacterial resistance and offers a promising strategy for developing antibiotics that minimize the evolution of resistance.
phys.org
March 3, 2025 at 10:02 PM
A dual-target antibiotic strategy, combining membrane disruption with another cellular pathway, shows promise in reducing bacterial resistance, offering a new direction in combating antimicrobial resistance. doi.org/g855sh
Reposted by Dipak Kathyat
Nature research paper: Macrophages recycle phagocytosed bacteria to fuel immunometabolic responses
https://go.nature.com/3XmyOPD
https://go.nature.com/3XmyOPD
Macrophages recycle phagocytosed bacteria to fuel immunometabolic responses - Nature
Phagocytosed bacteria can serve as an alternative nutrient source for macrophages, influencing their metabolic and immune responses through the recycling of microbial components, with the process regulated by nutrient-sensing mechanisms and bacterial viability.
go.nature.com
February 27, 2025 at 4:04 PM
Nature research paper: Macrophages recycle phagocytosed bacteria to fuel immunometabolic responses
https://go.nature.com/3XmyOPD
https://go.nature.com/3XmyOPD
Reposted by Dipak Kathyat
My first-author PhD paper is out in @naturecomms.bsky.social: A “double trouble” approach—combining membrane disruption with a key cellular pathway—dramatically limits bacterial #resistance. Discover “Exploring the principles behind antibiotics with limited resistance” #AMR #DrugDevelopment #MEvoSky
🚨 Our latest research in @naturecomms.bsky.social shows a promising strategy for less resistance-prone #antibiotics. For details, see the thread below and read the paper “Exploring the principles behind antibiotics with limited resistance” here: #MEvoSky www.nature.com/articles/s41...
Exploring the principles behind antibiotics with limited resistance - Nature Communications
This study shows that only those dual-targeting antibiotics limit resistance in Gram-negative pathogens that also target the membrane of the bacteria. This mechanism provides a basis for designing fut...
www.nature.com
February 25, 2025 at 11:02 AM
My first-author PhD paper is out in @naturecomms.bsky.social: A “double trouble” approach—combining membrane disruption with a key cellular pathway—dramatically limits bacterial #resistance. Discover “Exploring the principles behind antibiotics with limited resistance” #AMR #DrugDevelopment #MEvoSky
Reposted by Dipak Kathyat
The new JB is out. We thank ad hoc reviewers for 2024, minireviews on spores, mycobacteria, phages in the gut, formate metabolism and biofilm microenvironments. Check out articles on LD-transpeptidases, Myxo, B12 (my favorite vitamin) & more.
journals.asm.org/toc/jb/207/2
@asm.org #JBacteriology
journals.asm.org/toc/jb/207/2
@asm.org #JBacteriology
JB Table of Contents Volume 207, Issue 2
Journal of Bacteriology: Table of Contents Volume 207, Issue 2
journals.asm.org
February 21, 2025 at 2:46 PM
The new JB is out. We thank ad hoc reviewers for 2024, minireviews on spores, mycobacteria, phages in the gut, formate metabolism and biofilm microenvironments. Check out articles on LD-transpeptidases, Myxo, B12 (my favorite vitamin) & more.
journals.asm.org/toc/jb/207/2
@asm.org #JBacteriology
journals.asm.org/toc/jb/207/2
@asm.org #JBacteriology
Reposted by Dipak Kathyat
New review out in @NatureRevMicro on the complex rltnshp btween metabolism & AMR! A true labor of love from previous postbac Mehrose, grad students Varun & Zirui, and collaborator @drrobsmith.bsky.social (plus figures I'll likely use for many talks to come 😊) rdcu.be/eaES2
The role of bacterial metabolism in antimicrobial resistance
Nature Reviews Microbiology - In this Review, Ahmad et al. examine how antibiotics influence bacterial metabolism and how metabolism, in turn, affects drug efficacy and the emergence and evolution...
rdcu.be
February 20, 2025 at 10:37 PM
New review out in @NatureRevMicro on the complex rltnshp btween metabolism & AMR! A true labor of love from previous postbac Mehrose, grad students Varun & Zirui, and collaborator @drrobsmith.bsky.social (plus figures I'll likely use for many talks to come 😊) rdcu.be/eaES2
Excited to share my recent work published in #JBacteriology @asm.org ! We found that LD-transpeptidases drive phenotypic differentiation of Legionella and Coxiella into intrinsically resistant forms. Grateful to all who made this possible !
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
LD-transpeptidase-mediated cell envelope remodeling enables developmental transitions and survival in Coxiella burnetii and Legionella pneumophila | Journal of Bacteriology
Coxiella burnetii and L. pneumophila cause Q Fever and Legionnaire’s disease in humans, respectively. There is a lack
of effective treatments for fatal chronic infections caused by these pathogens, pa...
journals.asm.org
January 24, 2025 at 1:01 PM
Excited to share my recent work published in #JBacteriology @asm.org ! We found that LD-transpeptidases drive phenotypic differentiation of Legionella and Coxiella into intrinsically resistant forms. Grateful to all who made this possible !
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...